Genetic analysis for brix weight per stool and its component traits in sugarcane (Saccharum officinarum)

Brix weight per stool (BW) of sugarcane is a complex trait, which is the final product of a combination of many components. Diallel cross experiments were conducted during a period of two years for BW and its five component traits, in- cluding stalk diameter (SD), stalk length (SL), stalk number (SN), stalk weight (SW), and brix scale (BS) of sugarcane. Phenotypic data of all the six traits were analyzed by mixed linear model and their phenotype variances were portioned into additive (A), dominance (D), additive×environment interaction (AE) and dominance×environment interaction (DE) effects, and the correlations of A, D, AE and DE effects between BW and its components were estimated. Conditional analysis was employed to investigate the contribution of the components traits to the variances of A, D, AE and DE effects of BW. It was observed that the heritabilities of BW were significantly attributed to A, D and DE by 23.9%, 30.9% and 28.5%, respectively. The variance of A effect for BW was significantly affected by SL, SN and BS by 25.3%, 93.7% and 17.4%, respectively. The variances of D and DE effects for BW were also significantly influenced by all the five components by 5.1%～85.5%. These determinants might be helpful in sugarcane breeding and provide valuable information for multiple-trait improvement of BW.

Radiation-induced in vitro mutagenesis system for salt tolerance and other agronomic characters in sugarcane(Saccharum officinarum L.)

Gamma ray-induced in vitro mutagenesis and selection for salt(NaC l) tolerance were investigated in sugarcane(Saccharum officinarum L.). Embryogenic callus cultures were irradiated(10 to 80 Gy) and subjected to in vitro selection by exposure of irradiated callus to NaC l(0, 50, 100,150, 200, and 250 mmol L-1). Increasing NaC l concentrations resulted in growth reduction and increased membrane damage. Salt-selected callus lines were characterized by the accumulation of proline, glycine betaine, and Na+and K+concentration. Higher accumulation of proline and glycine betaine was observed in NaC l stressed callus irradiated at 20 Gy. Na+concentration increased and K+concentration decreased with increasing salt level. Irradiated callus showed50–60% regeneration under NaC l stress, and in vitro-regenerated plants were acclimatized in the greenhouse, with 80–85% survival. A total of 138 irradiated and salt-selected selections were grown to maturity and their agronomic performance was evaluated under normal and saline conditions. Of these, 18 mutant clones were characterized for different agro-morphological characters and some of the mutant clones exhibited improved sugar yield with increased Brix%,number of millable canes, and yield. The result suggest that radiation-induced mutagenesis offers an effective way to enhance genetic variation in sugarcane.

Databasing Molecular Identities of Sugarcane (Saccharum spp.) Clones Constructed with Microsatellite (SSR) DNA Markers

This paper reports the development of the first SSR marker-based sugarcane (Saccharum spp.) molecular identity database in the world. Since 2005, 1,025 sugarcane clones were genotyped, including 811 Louisiana, 45 Florida, 39 Texas, 130 foreign, and eight consultant/seed company clones. Genotyping was done on a fluorescence-capillary electrophoresis detection platform involving 21 highly polymorphic SSR markers that could potentially amplify 144 distinctive DNA fragments. Genotyping data were processed with the GeneMapper? software to reveal electrophoregrams that were manually checked against the 144 fragments. The presence (A) or absence (C) of these 144 fragments in any sugarcane clone was recorded in an affixed sequence order as a DNAMAN? file to represent its molecular identity being achieved into a local molecular identity database. The molecular identity database has been updated annually by continued genotyping of newly assigned sugarcane clones. The database provides molecular descriptions for new cultivar registration articles, enables sugarcane breeders to identify mis-labeled sugarcane clones in crossing programs and determine the paternity of cross progeny, and ensures the desired cultivars are grown in farmers’ fields.

甘蔗(Saccharum officinarum L.)品种遗传差异的AFLP分子标记分析

采用AFLP技术 ,从 6 4对引物中筛选出 5对带型分布均匀、多态性丰富且分辨能力强的引物 ,对两个在生产上广泛推广应用的甘蔗品种的AFLP指纹图谱进行分析 ,计算了两品种间的遗传相似性和遗传距离。结果表明 ,5对引物在 2个甘蔗品种间均存在显著的差异 ,其中多态性位点占总扩增位点的 10 2 % ,区分率达 10 0 %。这对应用AFLP技术鉴定甘蔗品种的真伪提供了依据。本文还对甘蔗AFLP分析体系的关键技术以及银染的安全性和可靠性进行了讨论。

Evaluation of Soil Quality Indicators in Sugarcane Management in Sandy Loam Soil

An important factor for the sustainability of soils highly susceptible to degradation is the use of monitoring tools that promptly and realistically reflect changes imposed on soil by different cropping systems. To select soil quality indicator variables in sugarcane (Saccharum officinarum L.) production areas that fulfill the criteria of sensitivity to management practices and between-season consistency in the management discrimination, ten composite soil samples (0–10 cm) were collected in July 2005 (rainy season) and again in March 2006 (dry season) from areas under cultivation of organic sugarcane (OS), green sugarcane (GS), burned sugarcane (BS) and from an adjacent native forest (NF) area at Usina Triunfo, Boca da Mata, Alagoas, Brazil. Microbial biomass-C (MBC), total organic C (TOC), soil enzyme activity expressed as the rate of fluorescein diacetate (FDA) hydrolysis, mean weight diameter of water-stable soil aggregates (MWD), and percentage of water-stable macroaggregates (PWSA) were analyzed. Although MBC and TOC were higher in NF than in the cultivated areas, no differences were observed in these C pools between the three sugarcane systems. The response of FDA to the site management was dependent on the sampling time. In the rainy period, the activity followed the order: NF > OS > GS > BS, whereas in the dry season, only NF differed from the other treatments. Irrespective of the sampling time, MWD and PWSA decreased in the order NF > OS = GS > BS. The variables MWD and PWSA are quite sensitive for discriminating between site management histories regardless the sampling season.

Implementation and Evaluation of Certain Properties of a Polymer Matrix Composite Material Reinforced by Fibrous Residues of <i>Saccharum officinarum</i>in View of an Applicability Orientation

This study examines the implementation and characterization of a polymer matrix composite material reinforced by sugarcane residues. The aim of the study is to enhance the abundantly produced sugarcane bagasse in the form of residues in the processing plants of said sugar cane. The composite material developed takes into account the size parameters and mass load rate of the reinforcement, the variations of which are between 2.5 mm and 4 mm respectively for the first parameter and 10% to 25% for the second. The load on the test tube during the test has a random orientation. The composite is polyester matrix. The cold compression moulding technique was used in the production of the various samples. Physical properties such as the rate of water absorption and the density of the composite are assessed. The three-point bending tests are carried out with the aim of inducing Young’s modulus from the elaborate samples. Analysis of physical properties shows that water absorption increases with the rate of residue load. The average water absorption rate of bagasse fibres is estimated at 8% for the 2.5 mm fiber size and 12% for the 4 mm fiber size for all composites. Mechanical characterization by bending tests reveals a fragile behavior of the samples tested. Young’s modulus decreases when the load rate of fibrous residues increases regardless of the size of the fibers.

<i>In Vitro</i>Priming of Sugarcane Varieties (RB966928 and RB867515)

Sugarcane, as a glycophyte, shows sensitivity to saline soils at various stages of its growth. The aim of this study was to evaluate the in vitro priming response in two sugarcane varieties (RB966928 and RB867515). Micropropagated plants, from meristems, received priming treatments by adding the salts (NaCl and KCl) in different concentrations (0.0;12.5;25.0 and 50.0 mM), in the MS medium. Subsequently, the plants were cultivated in rooting medium without addition of salts, acclimatized and submitted to gradient ex vitro saline stress with 20 → 40 and 60 mM, of each salt, for 30 days. The analyzed variables were dry matter of shoot and root, number of tillers and estimation of chlorophyll content. The experiment was carried out in a 2 × 2 × 4 factorial arrangement, in a completely randomized experimental design. Twenty replicates were used throughout the experiment. Data were submitted to analysis of variance and regression and the means were compared by Tukey’s test, at a 5% probability level. The priming treatments presented a significant effect, with triple interaction, in the chlorophyll index. In the treatment with NaCl, the variety RB966928 showed an increase in the chlorophyll index with the increase of treatment levels, up to an optimal limit of 31.47 mM. On the other hand, the variety RB867515 showed decreasing in chlorophyll index. In contrast, in KCl treatment, the variety RB867515 presented the increase at the chlorophyll index with the maximum point of 25 mM. For the variables, shoot dry matter (SDM) and root dry matter (RDM) there was a significant difference (p 0.05 and p 0.01, respectively) only between the varieties. The variety RB966928 presented higher SDM and RDM in relation to the variety RB867515. Studies are recommended with increasing the duration of the priming treatments and more detailed study of the culture throughout its productive cycle.

Characterization and Potential Evaluation of Residues from the Sugarcane Industry of Rio Grande do Sul in Biorefinery Processes

Brazil is the world largest producer of sugarcane (Saccharum officinarum) leading to a great generation of residues such as bagasse and straw, which represent two thirds of sugarcane energetic potential. Regarding these residues energetic potential, it is interesting to study their application in biorefinery processes. Thus, this work aimed at performing a chemical characterization of sugarcane straw and bagasse from RB867515 cultivar grown in Rio Grande do Sul-Brazil aiming at their use as feedstock in biorefinery processes. The obtained results were compared to data from other states and it was possible to conclude that edaphoclimatic conditions of Rio Grande do Sul have little influence in sugarcane residues chemical composition. Sugarcane bagasse presents larger potential for energetic use because of its volatiles content (74.82%) slightly higher than straw (68.90%), besides its high lignin content (21.85%) and higher calorific value (18.70 MJ/kg). Both sugarcane residues produced in Rio Grande do Sul have potential use as substrate in the obtainment of high value-added products from their cellulosic fractions (41.30% and 37.25%, respectively). The evaluation of energetic and chemical potential of sugarcane bagasse and straw produced in Rio Grande do Sul leads to the conclusion that these materials have high potential for use as feedstock in biorefineries.

Structural Changes of Lignified Tissues from Sugarcane Leaves Induced by Smut （Sporisorium scitamineum） Virulence Factors

Sugarcane leaf shows the classical arrangement of cells, which defines a C4 species. Vascular bundles consist of xylem, phloem and fibres, surrounded by an outer layer ofsclereids and an inner ring of stone cells associated with the phloem. Some sclereids located below and above the vascular bundles act as docking cells and connect the vascular bundle to the internal surfaces of upper and lower layers of the epidermis. A compact mass ofsclereids occupies the total internal volume of the leaf edge. Neither docking cells nor the internal mass of sclereids in the edge were markedly coloured by phloroglucinol, indicating the absence of lignin in their cell walls. However, such staining indicated that fibres of the vascular bundle and the external layer of sclereids were strongly lignified. Incubation of leaf discs with an virulence factors produced by the pathogen Sporisorium scitamineum increased the thickness of the lignified cell walls of sclereids as well as the mid and small xylem vessels, as a possible mechanical defence response to the potential entry of the pathogen. This mechanism was mainly revealed for the resistant cv. Mayari 55-14, whereas lignification decreased for the susceptible cv. B 42231.

Infestation of Diatraea saccharalis(Fabr.)during Five Consecutive Cycles of Sugarcane Crop Succeeding Leguminous Crops

The area cultivated with sugarcane in Brazil is expanding, and increasingly using legumes cover crop in new deployed areas and in those which were reformed. Long-term trials were carried out in Piracicaba, Sao Paulo, Brazil, to evaluate the effect of leguminous green manure on the natural infestation of Diatraea saccharalis (Fabr.) during five cropping cycles of sugarcane “IAC 87-3396”. This study was carried out from October 1999 to October 2005 in Piracicaba, Sao Paulo, Brazil, at coordinates 22°42''S and 47°38''W, 560 m above sea level, in soil classified as Typic Paleudult. Green manure crops were assigned to five treatments: peanuts (Arachis hypogaea L.) (cultivar “IAC-Tatu” and “IAC-Caiapó”), sunn hemp (Crotalaria juncea L.) (cultivar “IAC 1”) and velvet-bean [Mucuna aterrima (Piper & Tracy) Holland] (cultivar “common”). A treatment without any green manure or weed was used as the control. The previous sugarcane crop was destroyed and the soil was prepared in a conventional way (using plow and harrow) before the leguminous crops were sowed. Thirty days after the sowing, weeds were removed and, 120 days later, the crops were cut and its biomass was deposited on the soil surface without incorporation. Experimental plots comprised five rows of sugarcane, each one measuring 10 m long and spaced 1.40 m apart. The sugarcane was harvested 18 months after biomass deposit, on October 25th, 2001 and its stalks were collected at intervals of approximately 12 months in: 07/09/2002, 08/01/2003, 11/07/2004 and 10/06/2005. At harvest, the masses were determined from a sample of sugarcane, cut from three rows (2 m long) in the center of the plot. The losses caused by the sugarcane borer were estimated by the intensity of the infestation by randomly observing and collecting sugarcane stems from each plot. A great reduction in the number of stalks and in the yield was noted proportionally to the intensity of the infestation of the borer in the sugarcane crop grown after the velvet-bean. The sugarcane-velvet-bean rotation should be regarded considering the intensity of sugarcane borer infestation. No influence of the other green manure crops was observed on the intensity of the infestations. However, the harvest seasons of the sugarcane seemed to influence the borer infestation.

用AFLP分子标记技术鉴别甘蔗(Saccharum of ficinarum L.)品种

本研究采用AFLP技术 ,从 6 4对引物中筛选出 5对带型分布均匀、多态性丰富且分辨能力强的引物 ,对两个在生产上广泛推广应用的甘蔗品种的AFLP指纹图谱进行分析 ,计算了两品种间的遗传相似性和遗传距离。结果表明 ,5对引物在 2个甘蔗品种间均存在显著的差异 ,其中多态性位点占总扩增位点的 10 2 % ,区分率达 10 0 %。研究结果对生产上应用AFLP技术鉴定甘蔗品种的真伪性提供了依据。本文对甘蔗AFLP分析体系的关键技术以及银染技术的安全性和可靠性进行了讨论。

30份割手密种质资源苗期抗旱性综合评价

【目的】依据生理生化指标综合评价不同基因型割手密种质资源的苗期抗旱性,为甘蔗抗旱性育种的亲本选育提供参考。【方法】以30份割手密野生种质为试验材料进行盆栽试验,测定割手密苗期干旱胁迫处理前后的6项生理生化指标,采用主成分分析、模糊隶属函数分析和聚类分析对割手密的抗旱性进行综合评价。【结果】与正常供水相比,干旱胁迫下各割手密种质材料的过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、过氧化物酶(POD)活性增强,丙二醛(MDA)、可溶性糖(SS)、脯氨酸(Pro)含量增加,但各指标的变化程度因材料不同而存在一定差异。相关性分析结果表明,在干旱胁迫下,不同割手密种质材料叶片的过氧化氢酶活性和脯氨酸含量之间呈显著正相关(P<0.05),超氧化物歧化酶活性和脯氨酸之间呈极显著正相关(P<0.01);过氧化物酶活性和超氧化物歧化酶活性之间呈显著负相关;过氧化氢酶活性与丙二醛含量之间也呈显著负相关,其他指标之间无显著相关性(P>0.05)。主成分分析表明,SOD活性、POD活性、CAT活性和Pro含量、SS含量、MDA含量可作为割手密抗旱性评价的重要指标。通过模糊隶属函数分析和聚类分析可将30份割手密种质材料划分为4类,其中高度抗旱材料1份(90⁃53),中高度抗旱材料2份,中度抗旱材料14份,低度抗旱材料13份。【结论】CAT活性、SOD活性、POD活性、MDA含量、SS含量、Pro含量可作为割手密苗期的抗旱性评价指标。30份参试割手密种质中,90⁃53的抗旱性最强,2015⁃22、88⁃301次之,2015⁃104的抗旱性最弱。

桂西南蔗区甘蔗新品种筛选与评价

对8个甘蔗新品种(系)的农艺性状进行综合评价,筛选出优良品种(系),为桂西南蔗区特别是龙州蔗区新品种的推广提供参考。以ROC22为对照,对7个甘蔗新品种(系)进行一年新植两年宿根田间比较试验,采用DTOPSIS法对其进行筛选与评价。结果表明,各品种按照DTOPSIS分析结果Ci排序依次为:河糖11-157>桂糖10-1042、桂糖08-56>桂糖11-2320>桂糖09-439>ROC22>桂糖11-2119>桂糖10-417。甘蔗新品种河糖11-157和桂糖10-1042可在桂西南蔗区特别是龙州蔗区进行推广应用,桂糖08-56和桂糖09-439可作为优良新品种继续观察,桂糖11-2320、桂糖11-2119和桂糖10-417不建议在桂西南蔗区推广应用。

甘蔗属割手密(Saccharum Spontaneum),近缘属斑茅(Sclerostachya)及河八王(Narenga)的染色体数目研究

本文对来自云南、四川、福建、广东、贵州、江西的１０６份甘蔗野生种割手密无性系，以及甘蔗近缘属斑茅无性系２８份与河八王无性系３份的染色体进行观察计数。发现割手密染色体数目类型十分丰富有２ｎ＝６０、６４、７０、７２、７８、８０、９０、９２、９６、１０４、１０８，１１种类型，其中以２ｎ＝６４、８０、９６，３种类型出现频率最高，说明我国的割手密资源十分丰富。斑茅与河八王的染色体数目较为固定，分别为２ｎ＝６０，３０各１种类型。

两个割手密(Saccharum spontaneum L.)无性系对UV-B辐射响应的形态和生理差异

采用田间试验方法,研究了连续两年大田条件下,增强UV-B辐射(UV-B,280￣310nm,5.0kJ·m-2)对两个割手密(Saccharum spontaneum L.)无性系(Ⅰ91-48和Ⅱ91-81)形态和生理的影响,以及两个割手密无性系对UV-B辐射响应的种内差异。结果表明,两年中,在增强UV-B辐射下,Ⅰ91-485个生育时期的株高(除2004年成熟后期外)和分蘖数,以及分蘖期和伸长初期的茎径显著增加,而Ⅱ91-81株高在伸长初期和伸长后期,分蘖数在伸长初期、伸长后期、成熟初期和成熟后期(除2004年伸长后期外),以及茎径在分蘖期、伸长初期和伸长后期显著降低。两年的成熟后期,UV-B辐射导致Ⅰ91-48生物量、叶面积指数和节间长显著增加,Ⅱ91-81生物量、叶面积指数和节间长显著降低,而两个无性系节数无显著变化。两年中,UV-B辐射显著降低两个割手密无性系伸长后期和成熟初期叶展开角度。在UV-B辐射下,两年的5个生育时期中,Ⅰ91-48的类黄酮含量显著增加,丙二醛含量无显著变化,而Ⅱ91-81的类黄酮含量显著降低(除2003成熟后期外),丙二醛含量显著增加。总体上,UV-B辐射导致Ⅰ91-48叶绿素a、叶绿素b和叶绿素(a+b)含量在两年的分蘖期、伸长初期和伸长后期显著增加,而Ⅱ91-81叶绿素a、叶绿素b和叶绿素(a+b)含量在两年的分蘖期、伸长初期、伸长后期和成熟初期显著降低。割手密无性系Ⅰ91-48和Ⅱ91-81对UV-B辐射的响应具有种内差异。

甘蔗割手密种转录因子NAP亚家族的鉴定及SsNAP2a参与叶片衰老的功能分析

NAP(NAC-like,activated by apetala3/pistillata)是转录因子NAC基因家族中一类参与调控植物生长发育、叶片衰老及响应激素和非生物胁迫应答的亚家族。本研究利用甘蔗割手密种基因组数据和生物信息学方法,首先,基于比较基因组学对NAP亚家族成员进行鉴定、系统进化分析、保守结构域及顺式调控元件预测;其次,克隆获得割手密种SsNAP2的等位基因SsNAP2a,分析该基因在不同生长发育阶段的表达及其在激素和非生物胁迫下的表达特征;最后,利用瞬时表达和亚细胞定位分析SsNAP2a基因的功能。结果表明,在割手密种基因组中共鉴定5个NAP亚家族成员,亚细胞定位预测所有成员编码蛋白均定位于细胞核上,这些基因的Ka/Ks比值均小于1,表明纯化选择在演化中起到关键作用。系统聚类分析表明,5个代表性的被子植物(拟南芥、菠萝、水稻、玉米和高粱)与已报道的12个物种及甘蔗的NAP亚家族成员,共计46个,分为4个Clade,其演化的顺序Clade I>Clade II>Clade III>Clade IV。此外,在SsNAP亚家族成员的启动子区域预测到较多响应脱落酸和茉莉酸、低温等逆境胁迫的顺式作用元件,推测其参与多种激素和非生物胁迫相关应答通路。进一步,从割手密种SES208中克隆到SsNAP2a基因,该cDNA全长序列1173 bp(GenBank登录号为OQ335094),编码390个氨基酸残基,其与等位基因SsNAP2编码蛋白的序列相似性为97.70%,有10个氨基酸残基的差异,表明同源8倍体的割手密种等位基因序列差异较大。qRT-PCR分析表明,SsNAP2a基因在割手密种不同生长发育阶段中组成型表达,尤其在衰老的蔗皮和根中的表达量最高;在乙烯利(ethylene,ET)、脱落酸(abscisic acid,ABA)、4℃低温和40℃高温处理下,其表达量呈现显著诱导表达;而在8%聚乙二醇(polyethyleneglycol,PEG)胁迫下显著下调表达。亚细胞定位表明,SsNAP2a融合蛋白定位在细胞核上。瞬时过表达SsNAP2a基因7 d后,本氏烟(Nicotianabenthamiana)叶片有明显的卷曲、皱缩等衰老现象,ET合成相关基因(NtEFE26,NtAccdeaminase)显著上调表达,而水杨酸、茉莉酸和ABA合成相关基因(NtPR-1a/c、NtPR3和NtAREB1)显著下调表达,表明SsNAP2a基因参与多种激素信号传导途径诱发叶片衰老。以上研究结果为挖掘甘蔗NAP亚家族基因成员参与叶片衰老的生物学功能奠定了研究基础,也为培育甘蔗抗衰老分子育种提供候选的基因资源。

甘蔗的分类──Ⅱ．Saccharum L．属

Ｓａｃｃｈａｒｕｍ Ｌ．属的分类，经历了较大的变化。现在受到普遍接受的是Ｂｒａｎｄｅｓ（１９５８）的分类方法。他把ＳａｃｃｈａｒｕｍＬ．属分为６个种。本文讨论该属分类的历史衍变过程，各个种的形态特征、遗传特点、及对甘蔗育种的重要性。

田阳区甘蔗丰产栽培及病虫害综合防治技术

甘蔗是广西百色市田阳区特色产业,种植甘蔗是当地农民增收的重要途径之一,但若栽培技术不规范,病虫害防治不及时,将影响甘蔗的产量及质量。因此,做好甘蔗丰产栽培及病虫害综合防治意义重大。从甘蔗品种选择、蔗田选址、整地施肥、蔗种处理、蔗种下种、田间管理以及病虫害防治等方面,探讨了田阳区的甘蔗丰产栽培及病虫害综合防治技术,以为同类地区的甘蔗种植户提供借鉴。

甘蔗的分类──I·Saccharum的近缘植物

对甘蔗育种有重要利用价值的除Ｓａｃｃｈａｒｕｍ属外，还有一些与之关系密切的近缘植物．它们构成了“Ｓａｃｃｈａｒｕｍ复合群”，包括：Ｍｉｓｃａｎｔｈｕｓ属、Ｅｒｉａｎｔｈｕｓ属的Ｒｉｐｉｄｉｕｍ部分、Ｓｃｌｅｒｓｔａｃｈｙａ属、Ｎａｒｅｎｇａ属和Ｓａｃｃｈａｒｕｍ属。本文评述了这些植物的分类、分布、特征特性及对甘蔗育种的意义。

多元统计分析与投影寻踪法对甘蔗品质性状综合评价

本研究以新台糖22号及其7个杂交后代甘蔗新品种(系)为研究对象,利用多样性分析与投影寻踪法对甘蔗蔗糖分、纤维分等11个品质指标进行综合评价分析。结果表明,甘蔗蔗糖分与蔗汁直接转光度、蔗汁转化转光度呈极显著正相关,甘蔗纤维分与出汁率、甘蔗产糖量、蔗渣含水量呈极显著负相关,与蔗渣转光度呈显著正相关。通径分析结果表明,甘蔗糖分相关指标(蔗汁锤度、蔗汁蔗糖分、重力纯度、蔗汁直接转光度及煮液锤度等)对甘蔗蔗糖分具有直接正效应,蔗汁糖分及蔗渣煮液等相关指标(蔗汁锤度、重力纯度、蔗汁转化转光度、煮液转光度)对甘蔗纤维分具有直接正效应。对13个品质指标进行主成分分析,将其归为3个主成分,其特征值累积贡献率达85.71%。基于主成分分析及投影寻踪分类(PPC)分析,对不同甘蔗品质指标进行综合评价发现,桂糖09-439的综合评分值最高。聚类分析可将甘蔗品种(系)划分为三大类群,河糖11-157为第Ⅰ类群,为高糖高纤维分品种;桂糖09-439为第Ⅱ类群,为早熟高糖品种。本研究运用多元统计分析与投影寻踪法,都筛选出同一高糖甘蔗种质资源品种桂糖09-439、河糖11-157为高糖高纤维分品种,同时发现投影寻踪分类法可作为初筛大量甘蔗种质资源的途径之一。