Analysis of Occurrence and Mixed Infection of Sugarcane Bacilliform Virus Disease in Hainan Sugarcane-growing Area

[Objectives]The paper was to explore the occurrence and mixed infection of sugarcane bacilliform virus disease in Hainan sugarcane-growing area.[Methods]A total of 348 sugarcane leaf samples were collected from 7 sugarcane-growing areas in Hainan Province.Molecular detection of sugarcane bacilliform virus(SCBV)was carried out by PCR using specific primers.[Results]SCBV was detected in 244 out of 348 sugarcane samples,with an average detection rate of 70.11%.The highest detection rate was 76.66%in the Danzhou sugarcane-growing area,while the lowest was 57.14%in the Baisha sugarcane-growing area.The SCBV-positive samples were subjected to testing for SCYLV,SCSMV,SrMV,and SCMV,respectively.The results indicated that 106 out of 244 positive samples exhibited a single infection with SCBV,while 138 samples exhibited mixed infections with SCBV and other sugarcane viruses.The proportion of mixed infections among the SCBV-positive samples was as high as 56.56%.Among the various types of mixed infections,two-virus and three-virus mixed infections were the most prevalent.[Conclusions]SCBV has emerged as a significant threat to the secure production of sugarcane in the Hainan sugarcane-growing region.It presents an explosive infection in the Hainan sugarcane-growing region and frequently combines with other sugarcane viruses to infect sugarcane.The findings of this study will provide a theoretical foundation for the prevention and control of sugarcane bacilliform virus disease.

Virus Infection Situation of 37 Sugarcane Varieties in Guilin City

To understand virus infection situation of integrated demonstration and regional test sugarcane varieties in national sugarcane system in Guilin City,Guangxi Province,leaf samples with or without symptoms were collected from 37 sugarcane varieties,and five kinds of viruses were detected by RT-PCR with specific primers,including Sugarcane yellow leaf virus( SCYLV),Sugarcane streak mosaic virus( SCSMV),Sorghum mosaic virus( SrM V),Sugarcane mosaic virus( SCMV) and Sugarcane bacilliform virus( SCBV). The results showed that SCYLV was detected from four sugarcane varieties,and the detection rate was10. 81%; SCSMV was detected from five sugarcane varieties,and the detection rate was 13. 51%; SrM V was detected from four sugarcane varieties,and the detection rate was 10. 81%; SCMV had not been found; SCBV was detected from 27 sugarcane varieties,and the detection rate was 72. 9%. Additionally,mixed infection of different viruses was widespread,and the total mixed infection rate was 21. 62%. Thus,integrated demonstration and regional test sugarcane varieties in national sugarcane system in Guilin City had been seriously infected by viruses,and mixed infection of different viruses was common. The paper will provide a reference for breeding and popularization of antiviral sugarcane varieties.

桂北果蔗甘蔗杆状病毒的分子检测与分析

从桂北主栽果蔗区采集表现褪绿斑点或花叶及无症状的未脱毒拔地拉、青皮果蔗、紫皮果蔗、罗汉果蔗、广东黄皮、同安果蔗及脱毒拔地拉等7个品种蔗叶样品88份,用SCBV检测引物对采集的样品进行PCR检测分析。检测结果显示:有2份为SCBV阳性,检出率2.3%,均为未脱毒果蔗拔地拉,其中1份叶片无明显的症状;脱毒拔地拉、青皮果蔗、紫皮果蔗、罗汉果蔗、广东黄皮、同安果蔗检测均为阴性。表明桂北果蔗区存在SCBV侵染。

广西甘蔗杆状病毒的分子检测及分布调查

【目的】明确广西蔗区甘蔗杆状病毒病的发生及分布情况,了解不同甘蔗品种(品系)中甘蔗杆状病毒(Sugarcane bacilliform virus,SCBV)的侵染情况,为针对性地开展甘蔗杆状病毒病的防控提供理论依据。【方法】20122014年从广西14个地市的主要甘蔗种植区采集表现褪绿斑点、斑驳及无症状甘蔗叶片样品169份,同时从南宁的甘蔗育种和示范基地采集12个甘蔗品种(品系)叶片样品,采用杆状DNA病毒属(Badnavirus)通用检测引物Badna FP/RP对采集的样品进行PCR检测和测序分析。【结果】采自广西各地的169份样品中有105份为SCBV阳性,检出率达62.1%;样品中糖蔗与果蔗的SCBV阳性率相差不明显,分别为63.5%和58.1%;表现斑点或花叶的甘蔗叶片中SCBV的检出率较高,部分没有明显症状的甘蔗样品也检测到SCBV的存在。12个品种(品系)中包括新台糖系列、桂糖32、桂糖40、粤甘24、粤糖60和福农38等在内的7个甘蔗品种(品系)均检测到SCBV的核酸序列。【结论】目前广西蔗区存在SCBV侵染,且已经广泛分布。

52个甘蔗品种在广西受病毒侵染情况

为明确国家糖料体系甘蔗集成示范及区试品种在广西被病毒侵染情况,2017年从广西北海、南宁、崇左、百色、来宾、柳城、桂林等地集成示范及区试的52个甘蔗品种上采集带有甘蔗花叶病、甘蔗黄叶病和甘蔗杆状病毒病显著病症或不显病症叶片样品,采用特异引物通过RT-PCR和PCR方法进行5种病毒检测(甘蔗黄叶病毒、甘蔗线条花叶病毒、高粱花叶病毒、甘蔗花叶病毒和甘蔗杆状病毒)。结果表明,SCYLV的平均检出率为25.00%;SCSMV的平均检出率为7.97%;SrMV的平均检出率为7.69%;SCMV的平均检出率最低,为7.42%;SCBV的平均检出率最高,为68.41%,远远高于其他病毒。7个地方的甘蔗受病毒混合侵染现象普遍存在,北海的病毒混合侵染率甚至高于单一病毒侵染率。52个甘蔗品种在广西受5种病毒的总侵染率为79.67%,对甘蔗的生产安全存在着严重的潜在威胁,建议推广种植甘蔗脱毒种苗来缓解甘蔗病毒病害的危害。

Prevalence and Spatial Distribution of Badnavirus in the Banana (Musa spp) Major Growing Areas in Burkina Faso

Banana streak virus (BSV) and Sugarcane bacilliform virus (SCBV) are two badnaviruses commonly found in all banana growing areas of the world. It is a threat to the production and improvement of Musa germplasm. In Burkina Faso, the presence of badnaviruses was reported in banana producing regions. The objective of this study was to determine the prevalence of BSV and SCBV in banana production areas of Burkina Faso. A survey followed by a symptomatologic study was conducted in banana plantations in 27 localities of the nine main banana producing regions from July to October 2018 and September to December 2020. In all, 251 leaf samples were collected and analysed for BSV and SCBV infection by Indirect Antigen Coated Plate Assay-ELISA followed by amplification of the RT/RNase H region using Polymerase chain reaction with Badna FP/RP and SCBV F/R primers, respectively. A variety of symptoms were observed on almost all plant organs which were revealed due to BSV by symptomatologic study. The results of serological and molecular diagnosis revealed a high overall prevalence of BSV in 80.48% of the samples tested. BSV was distributed in seven survey regions out of nine with prevalence ranging from 10% to 100% in North, Centre, Centre West, Hauts Bassins, Cascades, Centre East and Boucle of Mouhoun regions. Very low prevalence was recorded for SCBV in Cascades and East Centre region with 4.35 and 12.5%, respectively. Species detection using specific primers to each species revealed three main species: Banana streak Obino l’ewaï virus (BSOLV), Goldfinger virus (BSGFV) and Imové virus (BSIMV) in the samples tested, respectively in the proportions of 23%, 8% and 0.8%. Co-infection between BSV species was also detected.