The photosensitive multilayer films from sulfonated metal-free, sulfonated copper-, and sulfonated nickel-phthalocyanines were fabricated with diazoresin layer by layer on a substrate via electrostatic interaction by ...The photosensitive multilayer films from sulfonated metal-free, sulfonated copper-, and sulfonated nickel-phthalocyanines were fabricated with diazoresin layer by layer on a substrate via electrostatic interaction by the self-assembly technique. Under UV irradiation, the linkage nature between the layers of the film is converted from the electrostatic bonding to covalent bonding. The covalently attached multilayer films are very stable towards polar solvents and salt aqueous solutions. The photovoltaic properties of the covalently attached film can be determined by means of a traditional three-electrode photoelectrochemical cell in aqueous solutions with KCl as the supporting electrolyte. The photocurrent determination has shown that the sulfonated copper-containing phthalocyanine films possess a higher photocurrent value than sulfonated metal-free and sulfonated nickel-containing phthalocyanine films.展开更多
Mouse hepatoma ascites was inoculated sc into the footpad of right hindleg. On d 7 after inoculation the tunior site was treated by heat (44 ℃, 25 min), photodynamic therapy (PDT) of aluminum sulfonated phthalocyanin...Mouse hepatoma ascites was inoculated sc into the footpad of right hindleg. On d 7 after inoculation the tunior site was treated by heat (44 ℃, 25 min), photodynamic therapy (PDT) of aluminum sulfonated phthalocyanine (AlSPc), and PDT plus heat. The hepatoma growth was almost completely inhibited and a high percentage of cure obtained in the combination group.The succinic dehydrogenase (SD), superoxide dismutase (SOD) and glutathione (GSH) of hepatonia cells were not affected by heat alone, but when heat was used following PDT, the SD, SOD and GSH in hepatonia calls were significantly inhibited.展开更多
The photosensitizing effects of sulfonated aluminum phthalocyanine on human liver cancer cells were studied by determining the kinetics of its cellular uptake, its state of aggregation therein and its photocytotoxicit...The photosensitizing effects of sulfonated aluminum phthalocyanine on human liver cancer cells were studied by determining the kinetics of its cellular uptake, its state of aggregation therein and its photocytotoxicitic effect on these cells. Fluorescence methods were used to measure the cellular uptake in cell extracts and intact cells in monolayer. Results exhibited that the cellular uptake increases with the incubation time of sulfonated aluminum phthalocyanine (Alspc) and saturates at 24 hours. This relation was in coincidence with that between Alspc's photocytotoxic effect and the Alspc incubation time. Although the average Alspc concentration in cells is higher than the incubation concentration of Alspc, laser fluorescence experiments showed that the fluorescence peak of Alspc in cells incubated in higher concentration coincides with that of its aqueous solution of low concentration, suggesting that Alspc in cancer cells exists in monomer state. The results that lipid peroxidation in cells is enhanced by Alspc photosensitization reflected that it may be one of the mechanisms of cell damage. The photodamage on cells was also studied with 3T3 mouse cells (conversion), showing agreeable results to that with liver cancer cells, which suggests that Alspc's photocytotoxic effect is nonselective to cell types.展开更多
基金Supported by the National Natural Science Foudation of China(No. 2 0 2 74 0 0 2 and 5 0 1730 0 2 )
文摘The photosensitive multilayer films from sulfonated metal-free, sulfonated copper-, and sulfonated nickel-phthalocyanines were fabricated with diazoresin layer by layer on a substrate via electrostatic interaction by the self-assembly technique. Under UV irradiation, the linkage nature between the layers of the film is converted from the electrostatic bonding to covalent bonding. The covalently attached multilayer films are very stable towards polar solvents and salt aqueous solutions. The photovoltaic properties of the covalently attached film can be determined by means of a traditional three-electrode photoelectrochemical cell in aqueous solutions with KCl as the supporting electrolyte. The photocurrent determination has shown that the sulfonated copper-containing phthalocyanine films possess a higher photocurrent value than sulfonated metal-free and sulfonated nickel-containing phthalocyanine films.
文摘Mouse hepatoma ascites was inoculated sc into the footpad of right hindleg. On d 7 after inoculation the tunior site was treated by heat (44 ℃, 25 min), photodynamic therapy (PDT) of aluminum sulfonated phthalocyanine (AlSPc), and PDT plus heat. The hepatoma growth was almost completely inhibited and a high percentage of cure obtained in the combination group.The succinic dehydrogenase (SD), superoxide dismutase (SOD) and glutathione (GSH) of hepatonia cells were not affected by heat alone, but when heat was used following PDT, the SD, SOD and GSH in hepatonia calls were significantly inhibited.
文摘The photosensitizing effects of sulfonated aluminum phthalocyanine on human liver cancer cells were studied by determining the kinetics of its cellular uptake, its state of aggregation therein and its photocytotoxicitic effect on these cells. Fluorescence methods were used to measure the cellular uptake in cell extracts and intact cells in monolayer. Results exhibited that the cellular uptake increases with the incubation time of sulfonated aluminum phthalocyanine (Alspc) and saturates at 24 hours. This relation was in coincidence with that between Alspc's photocytotoxic effect and the Alspc incubation time. Although the average Alspc concentration in cells is higher than the incubation concentration of Alspc, laser fluorescence experiments showed that the fluorescence peak of Alspc in cells incubated in higher concentration coincides with that of its aqueous solution of low concentration, suggesting that Alspc in cancer cells exists in monomer state. The results that lipid peroxidation in cells is enhanced by Alspc photosensitization reflected that it may be one of the mechanisms of cell damage. The photodamage on cells was also studied with 3T3 mouse cells (conversion), showing agreeable results to that with liver cancer cells, which suggests that Alspc's photocytotoxic effect is nonselective to cell types.
