This study was conducted to evaluate the degradation of phenolic compounds by one strain isolated from coal gasification wastewater( CGW). 16S rRNA gene sequences homology and phylogenetic analysis showed that the iso...This study was conducted to evaluate the degradation of phenolic compounds by one strain isolated from coal gasification wastewater( CGW). 16S rRNA gene sequences homology and phylogenetic analysis showed that the isolate is belonged to the genus Klebsiella sp. The effect of different phenolic compounds on the isolate was investigated by determining OD600and phenoloxidase activity,of which the results showed that the isolate can utilize phenol,4-methyl phenol,3,5-dimethyl phenol and resorcinol as carbon resources. The biofilm reactor( formed by the isolate) can resist the influent concentration of phenolic compounds as high as750 mg /L when fed with synthetic CGW and incubated at optimum conditions. The capacity of improving the biodegradability of CGW through degrading phenolic compounds was testified with fed the biofilm reactor with real CGW. Thus,it might be an effective strain for bioaugmentation of CGW treatment.展开更多
The improvement effect of bioaugmentation with phenol degrading bacteria( PDB) on pollutants removal and chemicals consumption was investigated in a full-scale Lurgi coal gasification wastewater( LCGW)treatment plant....The improvement effect of bioaugmentation with phenol degrading bacteria( PDB) on pollutants removal and chemicals consumption was investigated in a full-scale Lurgi coal gasification wastewater( LCGW)treatment plant. Bioaugmentation with PDB applied in biological contact oxidation tank( BCOT) was carried out in summer to prevent the limitation of low temperature on the bacteria activity. After augmentation,the removal of COD and total phenol(TPh) was significantly enhanced,with efficiencies from 78.5% and 80% to 82.3% and 86.6% in BCOT,respectively. The improvement could also be detected in further processes,including anoxic-oxic,coagulation sedimentation and biological aerated filter,with COD and TPh removal efficiencies increment from 70.1%,24. 7% and 53. 4% to 73. 9%,29. 1% and 55. 9%,from 67. 1%,20% and 25% to 72.5%,25% and 32%, respectively. In addition, chemicals used for denitrification and coagulation sedimentation showed considerable reduction after bioaugmentation,with methanol,coagulant,coagulant aid and bleaching dosage from 100. 0,100. 0,80. 0 and 60. 0 mg/L to 85. 0,70. 6,57. 8 and 45.7 mg/L,respectively. Therefore,bioaugmentation with PDB can be a viable alternative for LCGW treatment plant in pollutants removal and chemicals saving.展开更多
Twenty isolates of bacteria were isolated from contaminated wastewater collected from wastewater treatment station, used to clean the raw sewage at Jeddah on minimal medium containing phenol (0.5 g/l) as carbon source...Twenty isolates of bacteria were isolated from contaminated wastewater collected from wastewater treatment station, used to clean the raw sewage at Jeddah on minimal medium containing phenol (0.5 g/l) as carbon source while at 0.6 g/l phenol, five isolates belonging to Gram positive and Gram negative bacteria were obtained after 10 days of growth at 37°C. All the tested bacteria were tested for phenol degradation in liquid medium and isolate ABO11 was the most active isolate in phenol degradation. It was selected and tested for the ability to use phenol as carbon and energy source. It was identified as Bacillus sterothermophilus ABO11 using morphological and biochemical tests. Genetic studies confirmed the identification. Bacterial growth and phenol degradation by the selected bacterium were determined using different initial phenol concentrations (0 - 1.0 g/l). Very weak growth was recorded at 0.8 g/l and no growth or degradation was observed at higher phenol concentration. The isolate was adapted to grow in presence of 0.8 g/l phenol and phenol degradation was checked up to 12 days of growth. Percentage of degradation was 100% after 10 days. Maximum growth was observed at 40°C, pH 8 and using NH<sub>4</sub>Cl as nitrogen source. In conclusion, the selected isolate, Bacillus sterothermophilus ABO11 can be used in protecting the environment from phenol pollution.展开更多
基金Sponsored by the Sino-Dutch Research Program(SDRP)(Grant No.2012-2016)the State Key Laboratory of Urban Water Resource and Environment,Harbin Institute of Technology(Grant No.2013DX10)
文摘This study was conducted to evaluate the degradation of phenolic compounds by one strain isolated from coal gasification wastewater( CGW). 16S rRNA gene sequences homology and phylogenetic analysis showed that the isolate is belonged to the genus Klebsiella sp. The effect of different phenolic compounds on the isolate was investigated by determining OD600and phenoloxidase activity,of which the results showed that the isolate can utilize phenol,4-methyl phenol,3,5-dimethyl phenol and resorcinol as carbon resources. The biofilm reactor( formed by the isolate) can resist the influent concentration of phenolic compounds as high as750 mg /L when fed with synthetic CGW and incubated at optimum conditions. The capacity of improving the biodegradability of CGW through degrading phenolic compounds was testified with fed the biofilm reactor with real CGW. Thus,it might be an effective strain for bioaugmentation of CGW treatment.
基金Sponsored by China Postdoctoral Science Foundation(Grant No.2016M600254)
文摘The improvement effect of bioaugmentation with phenol degrading bacteria( PDB) on pollutants removal and chemicals consumption was investigated in a full-scale Lurgi coal gasification wastewater( LCGW)treatment plant. Bioaugmentation with PDB applied in biological contact oxidation tank( BCOT) was carried out in summer to prevent the limitation of low temperature on the bacteria activity. After augmentation,the removal of COD and total phenol(TPh) was significantly enhanced,with efficiencies from 78.5% and 80% to 82.3% and 86.6% in BCOT,respectively. The improvement could also be detected in further processes,including anoxic-oxic,coagulation sedimentation and biological aerated filter,with COD and TPh removal efficiencies increment from 70.1%,24. 7% and 53. 4% to 73. 9%,29. 1% and 55. 9%,from 67. 1%,20% and 25% to 72.5%,25% and 32%, respectively. In addition, chemicals used for denitrification and coagulation sedimentation showed considerable reduction after bioaugmentation,with methanol,coagulant,coagulant aid and bleaching dosage from 100. 0,100. 0,80. 0 and 60. 0 mg/L to 85. 0,70. 6,57. 8 and 45.7 mg/L,respectively. Therefore,bioaugmentation with PDB can be a viable alternative for LCGW treatment plant in pollutants removal and chemicals saving.
文摘Twenty isolates of bacteria were isolated from contaminated wastewater collected from wastewater treatment station, used to clean the raw sewage at Jeddah on minimal medium containing phenol (0.5 g/l) as carbon source while at 0.6 g/l phenol, five isolates belonging to Gram positive and Gram negative bacteria were obtained after 10 days of growth at 37°C. All the tested bacteria were tested for phenol degradation in liquid medium and isolate ABO11 was the most active isolate in phenol degradation. It was selected and tested for the ability to use phenol as carbon and energy source. It was identified as Bacillus sterothermophilus ABO11 using morphological and biochemical tests. Genetic studies confirmed the identification. Bacterial growth and phenol degradation by the selected bacterium were determined using different initial phenol concentrations (0 - 1.0 g/l). Very weak growth was recorded at 0.8 g/l and no growth or degradation was observed at higher phenol concentration. The isolate was adapted to grow in presence of 0.8 g/l phenol and phenol degradation was checked up to 12 days of growth. Percentage of degradation was 100% after 10 days. Maximum growth was observed at 40°C, pH 8 and using NH<sub>4</sub>Cl as nitrogen source. In conclusion, the selected isolate, Bacillus sterothermophilus ABO11 can be used in protecting the environment from phenol pollution.
