基于Super-GBS简化基因组测序技术的柞蚕SNP位点挖掘

基于Super-GBS基因分型(super-genotyping-by-sequencing)技术开展柞蚕单核苷酸多态性标记(single nucleotide polymorphism,SNP)位点挖掘研究,旨在开发性状关联SNP分子标记,为柞蚕种质资源评价鉴定、遗传图谱构建、QTL定位提供数据支撑。以白体色小白蚕为母本、黄体色H04为父本,获得BC1M群体(110个)、F_(1)(3个)、P_(1)(1个)、P_(2)(1个)总计115个柞蚕材料,利用PstⅠ-HF/MspⅠ双酶切基因组构建Super-GBS文库并测序,使用BWA软件将过滤后的序列数据比对到柞蚕参考基因组,采用GATK软件开发SNP标记,使用SnpEff软件注释SNP位点及R语言分析遗传结构。测序共产生序列数据量为106.39 Gb,过滤后的平均读长为0.89 Gb,Q30测序质量值平均为90.11%,比对率平均为98.48%。共得到有效SNP位点141100个,主要位于基因间隔区、内含子区、基因下游、上游,其中C/T、A/G变异类型最多,转换与颠换的比例为1.296187∶1。SNP位点变异主要为同义突变和错义突变,产生修饰(MODIFIER)影响。主成分与聚类分析将115个样品分为4组(P_(1)、P_(2)、F_(1)、BC_(1)M),反映了样品的遗传背景及亲缘关系,群体遗传分化指数F_(ST)在0.0003777~0.8272间,群体遗传距离DR分布在0.0004~1.7556,F_(ST)与DR均表现出明显的遗传背景上的聚类。结果表明,Super-GBS技术能够获得大量柞蚕SNP位点信息,可用于SNP标记开发,且开发的SNP标记能够对115个样品的亲缘关系、体色演变进行解释,为今后柞蚕种质资源评价与鉴定、分子标记辅助育种工作奠定基础。

基于简化基因组测序(Super-GBS)的子午岭黑山羊保种群遗传结构评估

旨在分析子午岭黑山羊的群体遗传多样性和亲缘关系以及家系结构,为子午岭黑山羊的保护和利用提供依据。本研究通过简化基因组测序(super-genotyping by sequencing,Super-GBS)技术对99只(10公/89母)成年子午岭黑山羊进行全基因组SNP检测。利用Plink软件计算观测杂合度(Ho)、期望杂合度(He)、多态信息含量(PIC)、核苷酸多样性(Pi)、有效等位基因数(Ne)及次要等位基因频率(MAF)等6项遗传多样性指标;GCTA软件进行主成分分析及基因组亲缘关系G矩阵构建;Plink软件构建IBS遗传距离矩阵,R语言绘制热图;PHYLP构建系统发育树;detect RUNS工具检测ROH。结果表明,99只子午岭黑山羊个体共检测到996042个SNPs。子午岭黑山羊群体的PIC、Pi、Ne及MAF值分别是0.161、0.193、1.295、0.130,且Ho(0.167)低于He(0.192),说明子午岭黑山羊群体遗传多样性较低。G矩阵和IBS遗传距离结果均表明子午岭黑山羊群体间大部分个体间亲缘关系较远。主成分分析结果揭示子午岭黑山羊群体内部不存在明显分化,系统发育树结果说明子午岭黑山羊群体公羊可大致分为6个家系,所有家系公羊数量较少。子午岭黑山羊群体的近交系数FROH为0.0496,说明子午岭黑山羊群体内部近交程度相对较低。综上所述,子午岭黑山羊群体遗传多样性较低,大部分个体间亲缘关系较远,群体内近交程度较低,后期应注意后代的选育,避免血统流失。

Super-Boost变换器的工作模式及输出纹波电压分析

Super-Boost变换器代替传统的充、放电模块可大大减小电源的质量和体积,提高其功率密度,因此在空间电力系统中具有广阔的应用前景。但由于Super-Boost变换器有多个功率元件,且存在电感电流反向流动特性,导致其供能模式及输出纹波电压较传统Boost变换器复杂。为了给Super-Boost变换器的分析和设计提供正确的理论指导,对Super-Boost变换器供能模式及输出纹波电压进行了深入研究。研究发现,电感L_(1)和L_(2)均存在连续导电模式、伪连续导电模式和伪断续导电模式等工作模式,建立各工作模式的临界电感和输出纹波电压解析数学模型,探讨峰值电流与电感间的关系,得到了满足设计要求的最小电容和最小电感,据此给出了变换器参数设计方法,实验结果验证了理论分析的正确性。

基于Super-Twisting滑模S面的无人机路径跟踪控制

针对小型固定翼无人机在执行任务时跟踪精度低以及容易受外界风影响的问题,设计基于Super-Twisting滑模S面(STSM S-Plane)的路径跟踪控制器,同时采用内外双环控制模式。外环即速度环采用Super-Twisting滑模控制,内环即姿态环采用S面控制。考虑到S面控制求导易导致积分爆炸的问题引入了二阶微分器,并对外界风组成进行建模研究。最后通过空间特殊曲线来验证所设计算法的控制性能。仿真结果表明,所设计的算法可以实现固定翼无人机对期望路径的精确跟踪,并具有良好的鲁棒性和抗干扰性能。

基于改进型Super-twisting的双馈感应发电机直接功率控制

为提高双馈感应发电机(DFIG)直接功率控制的鲁棒性和抗干扰能力,提出一种改进型超螺旋滑模(STSMC)的直接功率控制(DPC)。首先,建立参数摄动下的DFIG最大功率捕获模型和定子磁场定向的双馈感应发电机模型;然后,将非奇异快速终端滑模面(NFTSM)与一种改进型超螺旋(Super-twisting)优化算法结合,设计DFIG直接功率控制器(IST-NFTSMC)。其中所设计的非奇异滑模面能有效避免传统滑模控制(SMC)的奇异现象,且改进超螺旋控制律能有效削弱NFTSMC抖振,提高功率控制精度;最后,通过仿真和实验,与PI控制和传统SMC控制相比,该控制能削弱DFIG功率与电流抖振问题,加快其收敛速度,提高DFIG直接功率控制稳态精度。

组态视角下我国物流业绿色全要素生产率提升路径研究--基于Super-SBM-Malmquist与fsQCA方法

通过整合TOE框架,提取互联网发展水平、新能源比例、创新能力、物流业集聚程度、市场化水平和对外开放程度6个指标为前因变量,以物流业绿色全要素生产率为结果变量,将我国30个省(自治区、直辖市)数据作为研究样本,运用fsQCA方法揭示导致我国物流业产生高与非高绿色全要素生产率的组态路径。研究发现:“十三五”期间我国物流业绿色全要素生产率持续进步;大部分省市区的物流业绿色全要素生产率有提升;产生高物流业绿色全要素生产率的路径可归纳为“互联网-对外开放-创新”驱动型、“互联网-对外开放-新能源-物流业集聚”驱动型和“新能源-市场化-物流业集聚”驱动型三种。

TP347H与Super304H耐热钢热疲劳过程的变形与开裂行为研究

基于金属板材热疲劳试验方法(HB 6660-2011),对比分析了TP347H和Super304H两种耐热钢的供货态、相同热处理条件下的固溶态和时效态试样在热疲劳过程中底部尺寸的变形行为,以及缺口部位裂纹形貌。结果表明:这3种状态Super304H钢在热疲劳试验后的变形均小于相应TP347H钢的,TP347H缺口呈现相对较大的钝化,时效态试样钝化最大,裂纹最短。缺口附近的观察结果,TP347H在缺口附近龟裂损伤程度弱于Super304H钢的,开裂方式主要为沿晶开裂,也有部分穿晶开裂。

Selective internal radiation therapy segmentectomy:A new minimally invasive curative option for primary liver malignancies?

Transarterial radioembolization or selective internal radiation therapy(SIRT)has emerged as a minimally invasive approach for the treatment of tumors.This percutaneous technique involves the local,intra-arterial delivery of radioactive microspheres directly into the tumor.Historically employed as a palliative measure for liver malignancies,SIRT has gained traction over the past decade as a potential curative option,mirroring the increasing role of radiation segmentectomy.The latest update of the BCLC hepatocellular carcinoma guidelines recognizes SIRT as an effective treatment modality comparable to other local ablative methods,particularly well-suited for patients where surgical resection or ablation is not feasible.Radiation segmentectomy is a more selective approach,aiming to deliver high-dose radiation to one to three specific hepatic segments,while minimizing damage to surrounding healthy tissue.Future research efforts in radiation segmentectomy should prioritize optimizing radiation dosimetry and refining the technique for super-selective administration of radiospheres within the designated hepatic segments.

Efficacy of surgical resection and ultra-reduced tension suture combined with superficial radiation in keloid treatment

BACKGROUND There are many available treatment options for keloid;however,single treatments are usually less effective.Therefore,more scientifically rational and effective com-bined treatment methods should be sought to solve the pain associated with keloids.AIM To explore the efficacy and safety of surgical resection and ultra-reduced tension suture combined with superficial radiation as keloid treatment.METHODS Fifteen keloid patients admitted to Qingdao Eighth People's Hospital from June 2020 to January 2022 were enrolled in this retrospective analysis.All patients underwent a comprehensive treatment approach comprising surgical resection,ultra-reduced tension suture incision,and superficial radiation therapy within 24 h postoperatively.The modified Vancouver Scar Scale(mVSS)and Patient and Observer Scar Assessment Scale(POSAS)were used to evaluate the treatment effect,whereas the efficacy,adverse effects,and recurrence rate were observed according to the 12-mo follow-up after treatment.RESULTS The mVSS and POSAS scores at 1 and 6 mo after combination treatment decreased compared to before treatment(P<0.001),and the overall response rate was 93.3%.Only one case recurred,yielding a 6.7%recurrence rate.The incidence of local chromour sedimentation rate in 1–3 mo after radiotherapy was 33.3%(5 patients),all subsiding after 6–9 mo,without complications,such as delayed wound healing or dermatitis.CONCLUSION Surgical resection,super subtraction sutures,and superficial radiotherapy are treatment methods with short courses,low recurrence rates,and good safety profiles.

Image super‐resolution via dynamic network

Convolutional neural networks depend on deep network architectures to extract accurate information for image super‐resolution.However,obtained information of these con-volutional neural networks cannot completely express predicted high‐quality images for complex scenes.A dynamic network for image super‐resolution(DSRNet)is presented,which contains a residual enhancement block,wide enhancement block,feature refine-ment block and construction block.The residual enhancement block is composed of a residual enhanced architecture to facilitate hierarchical features for image super‐resolution.To enhance robustness of obtained super‐resolution model for complex scenes,a wide enhancement block achieves a dynamic architecture to learn more robust information to enhance applicability of an obtained super‐resolution model for varying scenes.To prevent interference of components in a wide enhancement block,a refine-ment block utilises a stacked architecture to accurately learn obtained features.Also,a residual learning operation is embedded in the refinement block to prevent long‐term dependency problem.Finally,a construction block is responsible for reconstructing high‐quality images.Designed heterogeneous architecture can not only facilitate richer structural information,but also be lightweight,which is suitable for mobile digital devices.Experimental results show that our method is more competitive in terms of performance,recovering time of image super‐resolution and complexity.The code of DSRNet can be obtained at https://github.com/hellloxiaotian/DSRNet.

New direction for surgery:Super minimally invasive surgery

The top goal of modern medicine is treating disease without destroying organ structures and making patients as healthy as they were before their sickness.Minimally invasive surgery(MIS)has dominated the surgical realm because of its lesser invasiveness.However,changes in anatomical structures of the body and reconstruction of internal organs or different organs are common after traditional surgery or MIS,decreasing the quality of life of patients post-operation.Thus,I propose a new treatment mode,super MIS(SMIS),which is defined as“curing a disease or lesion which used to be treated by MIS while preserving the integrity of the organs”.In this study,I describe the origin,definition,operative channels,advantages,and future perspectives of SMIS.

结合改进FPFH的Super-4PCS点云配准方法

针对超四点快速鲁棒匹配算法(Super-4PCS)粗匹配过程计算复杂度较高,配准时间长等问题,提出一种结合改进快速点特征直方图(FPFH)的Super-4PCS粗配准算法。通过主成分分析法(PCA)从快速点特征直方图中筛选出能代表点云特征信息的特征点,并将筛选出的特征点云作为输入数据进行Super-4PCS粗配准,由Super-4PCS粗配准得到初始变换矩阵,再进一步进行最近点迭代算法(ICP)精配准。为了验证在不同密度点云下的匹配效率,分别使用Bunny、Dragon两种不同密度的点云数据集进行配准实验,在满足精配准精度的基础上,对比FPFH-SAC和Super-4PCS粗配准方法,粗配准速率分别提升了72%和58%,总体配准速率分别提升了43%和32%。

Priming mesenchymal stem cells to develop “super stem cells”

The stem cell pre-treatment approaches at cellular and sub-cellular levels encompass physical manipulation of stem cells to growth factor treatment,genetic manipulation,and chemical and pharmacological treatment,each strategy having advantages and limitations.Most of these pre-treatment protocols are non-combinative.This editorial is a continuum of Li et al’s published article and Wan et al’s editorial focusing on the significance of pre-treatment strategies to enhance their stemness,immunoregulatory,and immunosuppressive properties.They have elaborated on the intricacies of the combinative pre-treatment protocol using pro-inflammatory cytokines and hypoxia.Applying a well-defined multi-pronged combinatorial strategy of mesenchymal stem cells(MSCs),pre-treatment based on the mechanistic understanding is expected to develop“Super MSCs”,which will create a transformative shift in MSC-based therapies in clinical settings,potentially revolutionizing the field.Once optimized,the standardized protocols may be used with slight modifications to pre-treat different stem cells to develop“super stem cells”with augmented stemness,functionality,and reparability for diverse clinical applications with better outcomes.

Simulation of the Ecosystem Productivity Responses to Aerosol Diffuse Radiation Fertilization Effects over the Pan-Arctic during 2001–19

The pan-Arctic is confronted with air pollution transported from lower latitudes.Observations have shown that aerosols help increase plant photosynthesis through the diffuse radiation fertilization effects(DRFEs).While such DRFEs have been explored at low to middle latitudes,the aerosol impacts on pan-Arctic ecosystems and the contributions by anthropogenic and natural emission sources remain less quantified.Here,we perform regional simulations at 0.2o×0.2ousing a well-validated vegetation model(Yale Interactive terrestrial Biosphere,YIBs)in combination with multi-source of observations to quantify the impacts of aerosol DRFEs on the net primary productivity(NPP)in the pan-Arctic during 2001-19.Results show that aerosol DRFEs increase pan-Arctic NPP by 2.19 Pg C(12.8%)yr^(-1)under clear-sky conditions,in which natural and anthropogenic sources contribute to 8.9% and 3.9%,respectively.Under all-sky conditions,such DRFEs are largely dampened by cloud to only 0.26 Pg C(1.24%)yr^(-1),with contributions of 0.65% by natural and 0.59% by anthropogenic species.Natural aerosols cause a positive NPP trend of 0.022% yr^(-1)following the increased fire activities in the pan-Arctic.In contrast,anthropogenic aerosols induce a negative trend of-0.01% yr^(-1)due to reduced emissions from the middle latitudes.Such trends in aerosol DRFEs show a turning point in the year of 2007 with more positive NPP trends by natural aerosols but negative NPP trends by anthropogenic aerosols thereafter.Though affected by modeling uncertainties,this study suggests a likely increasing impact of aerosols on terrestrial ecosystems in the pan-Arctic under global warming.

Extension of sound field reconstruction based on element radiation superposition method in a sparsity framework

Nearfield acoustic holography(NAH)is a powerful tool for realizing source identification and sound field reconstruction.The wave superposition(WS)-based NAH is appropriate for the spatially extended sources and does not require the complex numerical integrals.Equivalent source method(ESM),as a classical WS approach,is widely used due to its simplicity and efficiency.In the ESM,a virtual source surface is introduced,on which the virtual point sources are taken as the assumed sources,and an optimal retreat distance needs to be considered.A newly proposed WS-based approach,the element radiation superposition method(ERSM),uses piston surface source as the assumed source with no need to choose a virtual source surface.To satisfy the application conditions of piston pressure formula,the sizes of pistons are assumed to be as small as possible,which results in a large number of pistons and sampling points.In this paper,transfer matrix modes(TMMs),which are composed of the singular vectors of the vibro-acoustic transfer matrix,are used as the sparse basis of piston normal velocities.Then,the compressive ERSM based on TMMs is proposed.Compared with the conventional ERSM,the proposed method maintains a good pressure reconstruction when the number of sampling points and pistons are both reduced.Besides,the proposed method is compared with the compressive ESM in a mathematical sense.Both simulations and experiments for a rectangular plate demonstrate the advantage of the proposed method over the existing methods.

High-speed performance self-powered short wave ultraviolet radiation detectors based onκ(ε)-Ga_(2)O_(3)

High-speed solar-blind short wavelength ultraviolet radiation detectors based onκ(ε)-Ga_(2)O_(3)layers with Pt contacts were demonstrated and their properties were studied in detail.Theκ(ε)-Ga_(2)O_(3)layers were deposited by the halide vapor phase epitaxy on patterned GaN templates with sapphire substrates.The spectral dependencies of the photoelectric properties of struc-tures were analyzed in the wavelength interval 200-370 nm.The maximum photo to dark current ratio,responsivity,detectiv-ity and external quantum efficiency of structures were determined as:180.86 arb.un.,3.57 A/W,1.78×10^(12) Hz^(0.5)∙cm·W^(-1) and 2193.6%,respectively,at a wavelength of 200 nm and an applied voltage of 1 V.The enhancement of the photoresponse was caused by the decrease in the Schottky barrier at the Pt/κ(ε)-Ga_(2)O_(3)interface under ultraviolet exposure.The detectors demon-strated could functionalize in self-powered mode due to built-in electric field at the Pt/κ(ε)-Ga_(2)O_(3)interface.The responsivity and external quantum efficiency of the structures at a wavelength of 254 nm and zero applied voltage were 0.9 mA/W and 0.46%,respectively.The rise and decay times in self-powered mode did not exceed 100 ms.

Connotation,pathway and significance of carbon neutrality“super energy system”:A case study of the Ordos Basin,NW China

Super oil and gas basins provide the energy foundation for social progress and human development.In the context of climate change and carbon peak and carbon neutrality goals,constructing an integrated energy and carbon neutrality system that balances energy production and carbon reduction becomes crucial for the transformation of such basins.Under the framework of a green and intelligent energy system primarily based on“four news”,new energy,new electricity,new energy storage,and new intelligence,integrating a“super energy system”composed of a huge amount of underground resources of coal,oil,gas and heat highly overlapping with abundant wind and solar energy resources above ground,and a regional intelligent energy consumption system with coordinated development and utilization of fossil energy and new energy,with a carbon neutrality system centered around carbon cycling is essential.This paper aims to select the traditional oil and gas basins as“super energy basins”with the conditions to build world-class energy production and demonstration bases for carbon neutrality.The Ordos Basin has unique regional advantages,including abundant fossil fuel and new energy resources,as well as matching CO_(2)sources and sinks,position it as a carbon neutrality“super energy basin”which explores the path of transformation of traditional oil and gas basins.Under the integrated development concept and mode of“coal+oil+gas+new energy+carbon capture,utilization and storage(CCUS)/carbon capture and storage(CCS)”,the carbon neutrality in super energy basin is basically achieved,which enhance energy supply and contribute to the carbon peak and carbon neutrality goals,establish a modern energy industry and promote regional green and sustainable development.The pioneering construction of the world-class carbon neutrality“super energy system”demonstration basin in China represented by the Ordos Basin will reshape the new concept and new mode of exploration and development of super energy basins,which is of great significance to the global energy revolution under carbon neutrality.

Radiation of a TM mode from an open end of a three-layer dielectric capillary

Modern trends in beam-driven radiation sources include the interaction of Cherenkov wakefields in open-ended circular waveguides with complicated dielectric linings, with a three-layer dielectric capillary recently proposed to reduce radiation divergence being a representative example [Opt. Lett. 45 5416(2020)]. We present a rigorous approach that allows for an analytical description of the electromagnetic processes that occur when the structure is excited by a single waveguide TM mode. In other words, the corresponding canonical waveguide diffraction problem is solved in a rigorous formulation. This is a continuation of our previous papers which considered simpler cases with a homogeneous or two-layer dielectric filling. Here we use the same analytical approach based on the Wiener–Hopf–Fock technique and deal with the more complicated case of a three-layer dielectric lining. Using the obtained rigorous solution, we discuss the possibility of manipulating the far-field radiation pattern using a third layer made of a low permittivity material.

Prediction of multifaceted asymmetric radiation from the edge movement in density-limit disruptive plasmas on Experimental Advanced Superconducting Tokamak using random forest

Multifaceted asymmetric radiation from the edge(MARFE) movement which can cause density limit disruption is often encountered during high density operation on many tokamaks. Therefore, identifying and predicting MARFE movement is meaningful to mitigate or avoid density limit disruption for the steady-state high-density plasma operation. A machine learning method named random forest(RF) has been used to predict the MARFE movement based on the density ramp-up experiment in the 2022’s first campaign of Experimental Advanced Superconducting Tokamak(EAST). The RF model shows that besides Greenwald fraction which is the ratio of plasma density and Greenwald density limit, dβp/dt,H98and d Wmhd/dt are relatively important parameters for MARFE-movement prediction. Applying the RF model on test discharges, the test results show that the successful alarm rate for MARFE movement causing density limit disruption reaches ~ 85% with a minimum alarm time of ~ 40 ms and mean alarm time of ~ 700 ms. At the same time, the false alarm rate for non-disruptive and non-density-limit disruptive discharges can be kept below 5%. These results provide a reference to the prediction of MARFE movement in high density plasmas, which can help the avoidance or mitigation of density limit disruption in future fusion reactors.

Flexibility potential of Cs_(2)BX_(6)(B=Hf,Sn,Pt,Zr,Ti;X=I,Br,Cl)with application in photovoltaic devices and radiation detectors

As interest in double perovskites is growing,especially in applications like photovoltaic devices,understanding their mechanical properties is vital for device durability.Despite extensive exploration of structure and optical properties,research on mechanical aspects is limited.This article builds a vacancyordered double perovskite model,employing first-principles calculations to analyze mechanical,bonding,electronic,and optical properties.Results show Cs_(2)Hfl_(6),Cs_(2)SnBr_(6),Cs_(2)SnI_(6),and Cs_(2)PtBr_(6)have Young's moduli below 13 GPa,indicating flexibility.Geometric parameters explain flexibility variations with the changes of B and X site composition.Bonding characteristic exploration reveals the influence of B and X site electronegativity on mechanical strength.Cs_(2)SnBr_(6)and Cs_(2)PtBr_(6)are suitable for solar cells,while Cs_(2)HfI_(6)and Cs_(2)TiCl_(6)show potential for semi-transparent solar cells.Optical property calculations highlight the high light absorption coefficients of up to 3.5×10^(5) cm^(-1)for Cs_(2)HfI_(6)and Cs_(2)TiCl_(6).Solar cell simulation shows Cs_(2)PtBr_(6)achieves 22.4%of conversion effciency.Cs_(2)ZrCl_(6)holds promise for ionizing radiation detection with its 3.68 eV bandgap and high absorption coefficient.Vacancy-ordered double perovskites offer superior flexibility,providing valuable insights for designing stable and flexible devices.This understanding enhances the development of functional devices based on these perovskites,especially for applications requiring high stability and flexibility.