Smart Colorimetric Corn Starch Films Combined with Anthocyanin-Loaded Glutenin-Carboxymethyl Chitosan Nanocomplexes for Freshness Monitoring of Chilled Pork

In this study,intelligent,pH-responsive colorimetric films were prepared by encapsulating anthocyanins in nanocomplexes prepared from glutenin and carboxymethyl chitosan.These nanocomplexes were added to a corn starch matrix and used in the freshness monitoring of chilled pork.The effects of anthocyanin-loaded nanocomplexes on the physical,structural,and functional characteristics of the films were investigated.The addition of anthocyanin-loaded nanocomplexes increased the tensile strength,elongation at break,hydrophobicity,and light transmittance of the films while decreasing their water vapor permeability.This is because new hydrogen bonds are formed between the film components,resulting in a more homogeneous and dense structure.The colorimetric film has a significant color response to pH changes.These films were used in experiments on the freshness of chilled pork,in which the pH changes with changing freshness states.The results show that the colorimetric film can monitor changes in the freshness of chilled pork in real time,where orange,pink,and green represent the fresh,secondary fresh,and putrefied states of pork,respectively.Therefore,the intelligent colorimetric film developed in this study has good application potential in the food industry.

Temperature Trends and Accumulation of Chill Hours, Chill Units, and Chill Portions in South Carolina

There is considerable concern about the potential impact of climate change on agriculture, such as the accumulation of chilling hours needed to break the dormancy of many perennial plants, like fruit trees. Therefore, this study aimed to determine if there had been a significant change in air temperatures and chill hours, chill units, and chill portion accumulation in South Carolina over the last two decades. Two decades of daily maximum (Tmax) and minimum (Tmin) air temperature records were obtained from weather stations in thirty-one counties in South Carolina. Hourly temperature data, reconstructed from the daily data, were used to calculate the daily and annual chill hours, chill units, and chill portions accumulation using four different chill models for each location and year. The chill models included the T(t) °C model, the 0°C °C model, the Utah model, and the Dynamic model. For each county, regression analyses were conducted to evaluate the historical trends. Despite year-to-year variability, the tendency was a statistically significant (α = 0.05) increase in air temperature, averaging 0.089°C per year for 20 out of 31 counties in South Carolina. The other 11 counties had no significant change in temperature. The average temperature increase in the 31 counties was 0.072°C per year. The temperature increase resulted in a decrease in annual chill accumulation during the fall to spring, averaging 17.7 chill hours, 8.6 chill hours, 17.0 chill units, and 0.40 chill portions per year calculated with the T(t) °C, 0°C °C, Utah, and Dynamic models, respectively. However, whether this decrease in chill values was statistically significant or not depended on the chill model used. This study did not investigate the cause of the observed historical trends in temperature and chill accumulation. Still, if the trends continue, they could significantly impact the future of the temperate fruit tree industry in the state.

Changes in Chilled Chicken Quality and Correlation Analysis during Different Shelf Life

[Objective] The paper was to study the change rule and correlation analysis of quality indexes of chilled chicken within 7 d shelf life.[Method] Six pieces of fresh breast muscle were packaged in polyethylene（PE） food storage bags,labeled,and refrigerated at 4 ℃.The p H,water loss rate,tenderness,color value and inosinic acid content of chicken were measured at different time points of preservation（cold storage for 0,1,2,3,6,7 d）.[Result] With the prolongation of shelf life,the p H of chilled chicken first increased then tended to be stable;the water loss rate first increased then stabilized;the shear force first decreased then increased.There was little difference in brightness value（L＊） of chilled chicken dur-ing different shelf life（P〉0.05）.The red degree value（a＊） decreased with the prolongation of shelf life;the yellow degree value（b＊） increased with the extension of shelf life.The inosinic acid content decreased with the prolongation of shelf life.The p H of chilled chicken in different shelf life had significant positive correlation with water loss rate（P〈0.05） and extremely significant negative correlation with shear force（P〈0.01）.[Conclusion]The shelf life of 7 d has impact on meat color and inosinic acid of chilled chicken.Cold fresh preservation of chilled chicken should be less than 6 d;p H has certain correlation with water loss rate and shear force.

The Relationships between Rheological Properties and Structural Changes of Chilled Abalone Meat

The quantitative correlation between theological properties and structural characteristic values of chilled abalone meat was studied. Structural changes were observed, and these values were enumerated using image processing and analysis technique. Structural changes in the myofibrils and collagen fibrils were the greatest in chilling for 24 h. After chilling for 48 h, similar structures of vertical and cross sections were observed. For chilling from 0h to 72h, the instantaneous modulus E0 of the both section meat decreases gradually with time, but no significant differences were observed after chilling for 48 h.The relaxation time and viscosity of both sections attained the same values for the same chilling time, but increased gradually with increasing chilling time. Meanwhile, a negative correlation between the structural characteristic values (Dm, Am,Rvm), and rheological properties (E1,τi,η1) clearly exists. Some logarithmic expressions have been obtained for these negative correlations. These results suggest that the difference in rheological properties between the cross and vertical sections was mainly due to the structural changes of myofibrils and collagen fibrils, and rheological properties are influenced quantitatively by the structural characteristic values for chilling from 0 h to 72 h.

Fracture analysis of chilled cast iron camshaft

The fracture of a camshaft made of chilled cast iron,installed in a home-made Fukang car,happened only after running over a distance of 6,200 km.The fractured camshaft was received to conduct a series of failure analyses using visual inspection,SEM observation of fracture section,microstructure analysis,chemical composition analysis and hardness examination and so on,while those of CKD camshaft made by Citroēn Company in France was also simultaneously analyzed to compare the difference between them.The results showed that the fracture of the camshaft mainly results from white section in macrostructure and Ledeburite in microstructure;the crack in the fractured camshaft should be recognized to initiate at the boundary of coarser needle-like carbide and matrix,and then propagate through the transverse section.At the same time,the casting defects such as dendritic shrinkage,accumulated inclusion and initiated crack and abnormal external force might stimulate the fracture of camshaft as well.Based on failure analysis,some measures have been employed,and as a result,the fracture of home-made camshafts has been effectively prevented.

Exogenous melatonin improves the chilling tolerance and preharvest fruit shelf life in eggplant by affecting ROS-and senescence-related processes

Low temperature is the most common abiotic stress factors during the eggplant cultivation in solar greenhouses.Melatonin plays important roles in plant resistance to low temperature.However,the role of melatonin in regulating chilling tolerance and extending the preharvest shelf life of eggplant fruits is still unknown.In this study,we investigated the effects of exogenous melatonin on eggplant plants and fruits in response to low temperature.Under simulated low-temperature conditions,exogenous melatonin significantly relieved the chilling symptoms of seedlings by reducing reactive oxygen species (ROS) and malondialdehyde (MDA) levels and relative leakage rates.These reductions were caused by higher superoxide dismutase (SOD) and catalase (CAT) activities and increased endogenous polyamine and melatonin levels compared with those in untreated seedlings.Notably,the expression levels of SOD,CAT1/2,and polyamine synthesis genes (ADC and ODC) were also increased by 100μmol·L~(-1)melatonin,as well as those of genes involved in melatonin synthesis (TDC,T5H,SNAT,ASMT,and COMT) and cold regulation (COR1,CBFa/b,and ZAT2/6/12).To further investigate the effects of melatonin on eggplant leaves and fruits under natural low temperature conditions,100μmol·L~(-1)melatonin was sprayed on the functional leaves at three days before commodity maturation.Melatonin significantly alleviated chilling injury in the leaves and pericarp and extended the preharvest shelf life of the fruit by increasing the expression of COR1,CBF,ZAT2/6/12,and API5 and decreasing the expression of senescence-related genes (NCED1/2 and SAG12).Therefore,100μmol·L~(-1)melatonin improved chilling tolerance and fruit shelf life by upregulating ZAT2/6/12 to affect ROS-and senescence-related processes,which provides a reference for alleviating cold stress and extending the preharvest fruit shelf life in eggplant.

Effect of Nb on Structure and Mechanical Properties of Chilled Cast Iron at Room and Elevated Temperatures

Effect of Nb on microstructure and mechanical properties of chilled cast iron at room and elevated temperatures isstudied in this research. The results demonstrate that the cast structure and mechanical properties of chilled castiron at room and elevated temperatures are improved with the addition of trace amount of Nb. However, if Nb wasadded too much, the cast structure and mechanical properties of chilled cast iron would deteriorate. The suitablecontent of Nb in chilled cast iron is about 0.05% (mass fraction). Except the dissolution in the matrix of cast ironthe excessive Nb will form Nb-rich phases in three morphologies. Those are lumpy NbC, complicated strip-like phaseand compound with pearlite structure.

Effect of Lanthanun on micructure and properties of a chilled iron camshaft

Inoculation can reduce the chill depth of a chilled iron,and therefore influence the microstructure and properties of the iron.In this paper,the effect of rare earth Lanthanun (La) on the microstructure and properties of a chilled iron camshaft was studied.The results show that the La addition efficiently enhances the mechanical properties,yet with the unfavorable effect of decreasing the chilled depth and hardness.Moreover,La promotes graphite concentration and results in large graphite size,as well as A-type graphite.It is also found that excessive La destroys the interconnection and directivity of ledeburite.According to the experimental results,the optimum adding content of La should be no more than 0.02wt.%.

STUDY ON MORPHOLOGY OF CHROMIUM IN CHILLED Cu-0.14%-2.0%Cr ALLOYS

The morphology of chromium in chilled Cu-Cr alloys with 0.14%-2.0% Cr has been studied. The results showed that eutectic Cr phase takes a fibrous shape, and pre-eutectic Cr is dendritic in the studied chilled Cu-Cr alloy. During solute treatment of the eutectic and super-eutectic Cu-Cr alloys, only part of chromium particles dissolved in copper phase, some fiber and dendritic chromium still remained. Forging before solute treatment can reduce the size of primary Cr particles, which benefits the aging structure.

Extending Shelf Life of Chilled Pork by Combination of Chitosan Coating With Spice Extracts

The effects of spices（cinnamon,rosemary,clove）extracts and chitosan on microbiological growth,drip loss,color,and lipid oxidation of fresh chilled meat stored for 28 days at 4℃were investigated.There were four treatments：control,coated with spices（cinnamon 1.5 g·L^-1＋rosemary 1.5 g·L^-1＋clove 1.0 g·L^-1）,coated with 0.5%chitosan,coated with spices and chitosan.Chitosan coating resulted in significant inhibition of microbial growth（P〈0.05）,while the lowest microbial counts were obtained in the samples containing both chitosan and spices,indicating a possible synergistic effect.Chitosan and its combinations with spices also showed the most intense antioxidative effect when compared to the controls（P〈0.05）.Meanwhile,chitosan coating could decrease water loss and keep better color of chilled meat.The combined spice extracts and chitosan coating could effectively extend the shelf life of chilled meat.

Inhibition Effect of Herbal Preservatives on Listeria monocytogenes on Chilled Pork

This study investigated the growth situation of Listeria monocytogenes on chilled pork and the effect of herbal preservatives on this pathogen. The inhibitions of herbal preservatives were identified. The minimum inhibitory concentrations （MIC） of cinnamon and clove were all 0.79 mg.mL^-1, while the rosemary was 1.58 mg.mL^-1. And the composite herbal preservatives were got through orthogonal experiment. The optimum proportion was as following on agar medium： 1.16 mg.mL^-1 cinnamon＋2.38 mg. mL^-1 rosemary＋3.17 mg. mLl clove （herb combination number 5）, while on chilled pork, the strong inhibition of L. monocytogenes was showed, which demonstrated that the surface application of herb combination resulted in an effective delay of L. monocytogenes growth.

Genome-wide profiling of histone H3 lysine 27 trimethylation and its modification in response to chilling stress in grapevine leaves

Histone H3 lysine 27 trimethylation(H3K27me3) is a histone modification associated with transcriptional repression. However, insights into the genome-wide pattern of H3K27me3 in grapevines are limited. Here, anti-H3K27 chromatin immunoprecipitation(ChIP), high-throughput sequencing, and transcriptome analysis were performed using leaves of Vitis amurensis. The leaves were treated at 4°C for 2 h and 24 h and used to investigate changes in H3K27me3 under chilling treatment. The results show that H3K27me3 is well-distributed both in gene regions(-50%) and in the intergenic region(-50%) in the grapevine genome(Vitis vinifera ‘Pinot Noir PN40024'). H3K27me3 was found to be localized in8 368 annotated gene regions in all detected samples(leaves at normal temperature and under chilling treatments) and mainly enriched in gene bodies with the adjacent promoter and downstream areas. The short-term chilling treatments(4°C for 2 h) induced 2 793 gains and 305losses in H3K27me3 modification. Subsequently, 97.3% of the alterations were restored to original levels after 24 h treatment. The ChIP-qPCR for five differential peaks showed similar results to the data for ChIP-seq, indicating that the chilling-induced H3K27me3 modification is reliable.Integrative analysis of transcriptome and ChIP-seq results showed that the expression of H3K27me3 target genes was significantly lower than those of non-target genes, indicating transcriptional repression of H3K27me3 in grapevine leaves. Furthermore, histone methylation alterations were detected in 82 genes and were related to either repression or activation of their expression during chilling stress. The findings provide the genome-wide H3K27me3 patterns in grapevines and shed light on uncovering its regulation in chilling stress responses.

Microstructure, Mechanical Properties (Strength and Hardness) and Wear Behavior of Chilled Aluminum Alloy (LM-13) Reinforced with Titanium Carbide (TiC) Metal Matrix Composites

This paper deals with the production of aluminum alloy (LM-13) reinforced with titanium carbide (TiC) chilled composites subject to different chilling rates to study the effect of chilling on microstructure, mechanical and tribological behavior. Metallic and non-metallic chills (25 mm thick) were used in this investigation. The specimens taken from casting blocks were tested for their microstructure, strength, hardness and wear behavior. It was found in the present investigation that chilling rate and addition of TiC reinforcement (3 to 12 wt% in steps of 3 wt%) have improved both mechanical properties (strength and hardness) and wear resistance of the composite developed. Out of the chills used, copper chill was found to be good in improving mechanical properties because of its high Volumetric Heat Capacity (VHC). It is concluded from the above investigation that type of chill and hence chilling rate, reinforcement content have an effect on mechanical properties wear behavior.

Fabrication and Corrosion Behaviour of Aluminium Alloy (LM-13) Reinforced with Nano-ZrO₂Particulate Chilled Nano Metal Matrix Composites (CNMMCs) for Aerospace Applications

This research presents the results obtained and the discussions made from a series of corrosion experiments involving aluminum alloy (LM 13) reinforced with Nano-ZrO2, size of the particles dispersed varies from 100 - 200 nm and amount of addition varies from 3 wt% to 15 wt% in steps of 3 wt%. The resulting CNMMCs are solidified under the influence of copper chill of 25 mm thickness to study the effect of corrosion behavior. The corrosion test employed was the electrochemical polarization method according to ASTM G59-97 (2009) standards. Corrosion resistance was found to increase significantly with increase inZrO2 content in chilled CNMMCs. Nevertheless, even with high ZrO2 content corrosion attack i.e., pitting was found to be most severe during the initial stages of each test but it invariably decreased to a very low value in the later stages, due to the formation of an adherent protective layer on the CNMMCs developed. SEM studies of the corroded surface were also examined to study the mechanism of corrosion. Present research is undertaken to develop a corrosion resistant composite for aerospace application where aluminum is highly suspected to corrosion.

Melatonin treatment alleviates chilling injury in mango fruit 'Keitt' by modulating proline metabolism under chilling stress

Mangoes often suffer from low temperature-induced chilling injury (CI) during postharvest cold storage.Therefore,advanced techniques are crucial and in high demand to solve the chilling stress of mango fruit for a higher value.This study addresses chilling stress modulation by investigating the effects of melatonin treatment on CI,proline metabolism,and related gene expressions of ‘Keitt’ mango during cold storage after dipped in 0 (control),0.1 (MT1),and 0.2 mmol L^(-1)(MT2) melatonin solution for 30 min.The results revealed that melatonin treatment in MT1 significantly reduced CI development and increased proline content in mango fruit during cold storage compared to the control.These changes were along with increases in the activity of critical enzymes as well as the expression of encoding genes involved in proline biosynthesis,such as pyrroline-5-carboxylate synthetase (P5CS),pyrroline-5-carboxylate reductase (P5CR),ornithine D-aminotransferase (OAT),P5CS2,P5CR2,and OAT3.Additionally,proline dehydrogenase (PDH) activity and the expression of the PDH3 gene associated with proline dehydrogenation were lower in MT1-treated mangoes than the controlled group.Thus,melatonin treatment has regulated proline metabolism resulting in the accumulation of proline,subsequently contributing to enhancing the chilling tolerance of‘Keitt’mango fruit.

Numerical evaluation of the effectiveness of frost heave mitigation strategies for chilled arctic gas pipelines

To prevent the thawing of ice-rich permafrost,it is suggested that gas should be transported in a chilled state(below the freezing temperature)in pipelines buried in permafrost.However,frost heave occurs when water migrates towards the chilled pipeline and ice lenses grow underneath the pipe.This might endanger the integrity of the pipeline and the environment as well.Therefore,innovative frost heave mitigation measures are required when designing the pipeline,especially those sections in discontinuous permafrost or near the compressor stations.The ground temperature field in response to the operation of a proposed chilled gas pipeline traversing permafrost regions in Alaska was simulated by a pipe-soil thermal interaction geothermal model.Frost heave mitigation measures,including insulation around the pipe,flat slab insulation under the pipe,and heating cables combined with slab insulation,were evaluated for chilled pipeline operation in seasonally varying ambient temperatures.The numerical results show that the minimum temperature of the observation point at 2.5 m below the pipe bottom increases by 17%,29%,and 48%when the thermal conductivity of the outer insulation layer is 0.1,0.05,and 0.02 W/(m K),respectively.For flat slab insulation,the thermal field is less sensitive to varying slab thicknesses than to varying thermal conductivity,implying the thermal conductivity,not the thickness,is the crucial factor.Additionally,the heat flow could be redirected from vertical to horizontal by flat slab insulation.The electrical heating cables could be regarded as a new heat source to balance the heat removal rate of the soil around the chilled pipe.The minimum temperature of the observation point at 1.1 m below the bottom of the pipe increases from-15.2℃to-3.0,1.5,and 7.5℃,corresponding to the heating cable power of 20,30,and 40 W,respectively,with the power of 30 W deemed appropriate for the study case.It is concluded that heating cables in combination with insulation slabs could be adopted to regulate the temperature field around the chilled pipeline efficiently and economically.The advantages of this combination include redirecting the heat flow and eliminating frost in the soil underlying the pipe.These approaches could be considered for applications in gas pipeline projects in arctic and alpine/high-plateau permafrost regions.

Surface Waviness in Grinding of Thin Mould Insert Using Chilled Air as Coolant

On going trend of miniaturization in electronic rel at ed parts, which is an average of two times in every 5～7 years introduce grindin g challenges. In grinding process, the surface waviness control of thin parts is an ardent task due to its warpage, induced by the high specific grinding energy (2～10 J/mm 3). Therefore, coolant is often used to avoid thermal damage, obtai n better surface integrity and to prolong wheel life. However coolant, the incomp ressibility media introduce high forces at the grinding zone creating dimensiona l as well as shape instability. In view of these situations chilled air was ap plied in place of conventional coolant. The chilled air is produced using a two -stage vapor compression refrigeration cycle with characteristics of: temperatu re -35 ℃, pressure 0.2～0.3 MPa and flow rate 0.4 m 3/min. Also traces of eco - oil mist that encompass the chilled air are supplied to the grinding zone. B oth chilled air and eco-oil mist are applied through two independent paths of a specially designed twin compartment nozzle for maximizing the penetration. This paper investigates the grinding characteristics of mold insert which is closer to M2 tool steel (component widely used in connector industries) when using chil led air as coolant media. Grinding experiments were conducted using a vitrified bond CBN wheel (B91N100V) and a surface grinder. Initial study was focussed on establishing the most suita ble clamping method for the thin mold insert. FEM analysis and grinding experime nt studies were performed to quantitatively analyze the clamping induced deflect ion. Waviness value (W t) of (24～62) μm was achieved for resin clampi n g whereas (4～8) μm, (4～6) μm were achieved for magnetic and wax clamping res pe ctively. Wax clamping is predominantly used in all the grinding experiments that characterize the grinding process, which use chilled air as the coolant media. Between 0.15 to 0.9 mm 3/mm.s of specific material removal rate, ground sur face temperature of mold insert was increased from 0.3 ℃ to 59.7 ℃ for chi lled air. For the similar grinding conditions with the coolant fluid an increase from 0.9 ℃ to 14.4 ℃ was recorded. With increase of specific material removal rate from 0.15 to 0.65 mm 3/mm.s, F t/F n ratio was increased from (0.2 to 0.4), (0.6 to 1.67) for wet coolant and chilled air respectively. Despite of high F t/F n ratio and ground surface temperature, chilled air method has shown a surface waviness, W t from (2 to 5.6) μm. Microstructure examination of chilled air produced ground surface was comparable to those of using coolant fluids. Surface finish, R a of (0.45～0.7) μm was achieved for mold insert . This work will enable to have clear understanding about the quantitative influe nce of chilled air as well as the clamping method against the surface waviness o f thin mold insert.

Development of Nickel Alloy Reinforced with Fused SiO2 Chilled Composites and Evaluation of Thermal Properties (Thermal Conductivity &Coefficient of Thermal Expansion) and Temperature Distribution by Finite Element Analysis (FEA)

This paper presents the results obtained and the discussions made from a series of thermal experiments involving Nickel alloy (ASTM A 494 M) reinforced with fused SiO2, and size of the particles dispersed varies from 80 - 120 μm and amount of addition varies from 3 to 12 wt.% in steps of 3 wt.%. The resulting chilled MMCs are solidified under the influence of copper chill of 25 mm thickness to study the effect of chilling on thermal behaviour. Microstructural studies indicated that the reinforcement distribution is uniform with very good bonding due to chilling effect. Thermal properties were found to decrease significantly with increase in SiO2 content in chilled MMCs. It is concluded from the research that reinforcement content and the temperature has an effect on coefficient of thermal expansion and thermal conductivity of the chilled composite developed. Finite element analysis of the exhaust valve of the IC indicates that chilled Ni alloy composite developed in the present research can be used as alternate material for the existing valve material (Ni-Cr alloy steel). All the tests conducted in this research are in conformance with ASTM standards.

Down-regulation of MeMYB2 leads to anthocyanin accumulation and increases chilling tolerance in cassava(Manihot esculenta Crantz)

Chilling-induced accumulation of reactive oxygen species(ROS) is harmful to plants,which usually produce anthocyanins to scavenge ROS as protection from chilling stress.As a tropical crop,cassava is hypersensitive to chilling,but the biochemical basis of this hypersensitivity remains unclear.We previously generated Me MYB2-RNAi transgenic cassava with increased chilling tolerance.Here we report that Me MYB2-RNAi transgenic cassava accumulated less ROS but more cyanidin-3-O-glucoside than the wild type under early chilling stress.Under this stress,the anthocyanin biosynthesis pathway was more active in Me MYB2-RNAi lines than in the wild type,and several genes involved in the pathway,including Me TT8,were up-regulated by Me MYB2-RNAi in the transgenic cassava.Me MYB2 bound to the Me TT8 promoter and blocked its expression under both normal and chilling conditions,thereby inhibiting anthocyanin accumulation.Me TT8 was shown to bind to the promoter of Dihydroflavonol 4-reductase(Me DFR-2)and increased Me DFR-2 expression.Me MYB2 appears to act as an inhibitor of chilling-induced anthocyanin accumulation during the rapid response of cassava to chilling stress.

菠萝抗寒研究进展

低温是限制菠萝产量和品质的重要因素之一。我国菠萝种植适宜区冬季周期性地受到低温等自然灾害影响,且近年来频繁遭遇严峻气候环境挑战,造成减产或失收,严重影响菠萝产业可持续发展。目前,关于菠萝抗寒性研究主要集中于抗寒种质资源鉴定与评价、抗寒性评价方法、生理生化测定、抗寒基因挖掘和抗寒防治措施等方面,而在菠萝抗寒性分子机制方面的研究进展较缓慢,本文针对这些方面进行概述,以期为菠萝抗寒性状的遗传改良及生产上防寒防冻提供参考。