葡萄休眠相关基因DAMs的克隆分析及对单氰胺的响应

DAM基因是真核生物转录因子之一,在木本果树的芽休眠中起着重要的调控作用,且在不同物种间数量不同。以‘水晶’葡萄为试材,于冬芽休眠期间采集枝条后均匀涂抹2.5%单氰胺,在处理后的不同时间采集冬芽,用于克隆VvDAMs基因序列,对其进行生物信息学和荧光定量分析,并测定冬芽激素含量。结果表明,克隆获得的VvDAM1、VvDAM2和VvDAM3基因的开放阅读框的长度分别为690、696、702 bp,分别编码229、231、233个氨基酸和1个终止密码子,相对分子量分别为25.96、26.76、25.99 kD,理论等电点分别为7.71、10.49、9.10;三者的二级结构形式均呈现α-螺旋>无规则卷曲>延伸链的规律;VvDAM1、VvDAM2、VvDAM3氨基酸序列上有一定的同一性,VvDAM1与欧洲李PsDAM1、VvDAM2与樱桃李PcDAM2、VvDAM3与李PsDAM3之间的同源性均达到98%以上;此外,冬芽休眠解除期间,VvDAM 1、VvDAM 2、VvDAM 3基因的表达量逐渐下降,IAA、GA3+4、ZR的含量和GA3+4/ABA逐渐上升,ABA含量和IAA/ZR逐渐下降,单氰胺处理后使其变化提前且幅度增大,从而使芽提前萌发,为葡萄冬芽破眠、早熟栽培等提供理论依据和实践意义。

Numerical investigation of hydro-morphodynamic characteristics of a cascading failure of landslide dams

A cascading failure of landslide dams caused by strong earthquakes or torrential rains in mountainous river valleys can pose great threats to people’s lives,properties,and infrastructures.In this study,based on the three-dimensional Reynoldsaveraged Navier-Stokes equations(RANS),the renormalization group(RNG)k-εturbulence model,suspended and bed load transport equations,and the instability discriminant formula of dam breach side slope,and the explicit finite volume method(FVM),a detailed numerical simulation model for calculating the hydro-morphodynamic characteristics of cascading dam breach process has been developed.The developed numerical model can simulate the breach hydrograph and the dam breach morphology evolution during the cascading failure process of landslide dams.A model test of the breaches of two cascading landslide dams has been used as the validation case.The comparison of the calculated and measured results indicates that the breach hydrograph and the breach morphology evolution process of the upstream and downstream dams are generally consistent with each other,and the relative errors of the key breaching parameters,i.e.,the peak breach flow and the time to peak of each dam,are less than±5%.Further,the comparison of the breach hydrographs of the upstream and downstream dams shows that there is an amplification effect of the breach flood on the cascading landslide dam failures.Three key parameters,i.e.,the distance between the upstream and the downstream dams,the river channel slope,and the downstream dam height,have been used to study the flood amplification effect.The parameter sensitivity analyses show that the peak breach flow at the downstream dam decreases with increasing distance between the upstream and the downstream dams,and the downstream dam height.Further,the peak breach flow at the downstream dam first increases and then decreases with steepening of the river channel slope.When the flood caused by the upstream dam failure flows to the downstream dam,it can produce a surge wave that overtops and erodes the dam crest,resulting in a lowering of the dam crest elevation.This has an impact on the failure occurrence time and the peak breach flow of the downstream dam.The influence of the surge wave on the downstream dam failure process is related to the volume of water that overtops the dam crest and the erosion characteristics of dam material.Moreover,the cascading failure case of the Xiaogangjian and Lower Xiaogangjian landslide dams has also been used as the representative case for validating the model.In comparisons of the calculated and measured breach hydrographs and final breach morphologies,the relative errors of the key dam breaching parameters are all within±10%,which verify the rationality of the model is applicable to real-world cases.Overall,the numerical model developed in this study can provide important technical support for the risk assessment and emergency treatment of failures of cascading landslide dams.

Impacts of artificial dams on terrestrial water storage changes and the Earth's elastic load response during 1950-2016: A case study of medium and large reservoirs in Chinese mainland

The construction of dams for intercepting and storing water has altered surface water distributions, landsea water exchanges, and the load response of the solid Earth. The lack of accurate estimation of reservoir properties through the land surface and hydrological models can lead to water storage simulation and extraction errors. This impact is particularly evident in many artificial reservoirs in China. The study aims to comprehensively assess the spatiotemporal distribution and trends of water storage in medium and large reservoirs(MLRs) in Chinese mainland during 1950-2016, and to investigate the gravity,displacement, and strain effects induced by the reservoir mass concentration using the load elasticity theory. In addition, the impoundment contributions of MLRs to the relative sea level changes were assessed using a sea-level equation. The results show impoundment increases in the MLRs during1950-2016, particularly in the Yangtze River(Changjiang) and southern basins, causing significant elastic load effects in the surrounding areas of the reservoirs and increasing the relative sea level in China's offshore. However, long-term groundwater estimation trends are overestimated and underestimated in the Yangtze River and southwestern basins, respectively, due to the neglect of the MLRs impacts or the uncertainty of the hydrological model's output(e.g., soil moisture, etc.). The construction of MLRs may reduce the water mass input from land to the ocean, thus slowing global sea level rise. The results of the impact of human activities on the regional water cycle provide important references and data support for improving the integration of hydrological models, evaluating Earth's viscoelastic responses under longterm reservoir storage, enhancing in-situ and satellite geodetic measurements, and identifying the main factors driving sea level changes.

Environmental and Human Impacts of Lancang-Mekong Mainstem and Tributary Dams on China, Laos, Thailand, Myanmar, Cambodia, and Vietnam

The Lancang-Mekong River in China, Laos, Thailand, Myanmar, Cambodia, and Vietnam is the soul and heart of mainland Southeast Asia. Over 60 million people depend on the river and its tributaries for food, transportation, water, and other necessities of life. The river supports one of the world’s most diverse fisheries, second only to Brazil’s Amazon River. Lancang-Mekong and tributaries are already heavily dammed primarily in China, Laos, Thailand, and Cambodia, with many more dams planned or under construction. Dams can worsen the impact of periodic droughts in the Lancang-Mekong basin and block the river’s “pulse effect” that spreads water and nutrients needed for fishing and farming onto the floodplains and delta. The headwaters of the Lancang are in China and its waters are considered a national resource. China regards the Lancang, Yangtze and Yellow rivers as a free resource rather than a shared resource. The primary difference between these rivers is the Lancang flows from China into and through other countries and not directly into a sea or ocean. China and Myanmar have not joined the Mekong River Commission (MRC) as full members but have been Dialogue Partners since 1996. Over the past thirty years, China’s Lancang policies and actions have reflected its national resource interests. China has actively engaged with individual transboundary countries at various levels including environmental, conservation, and economic agreements. The primary objective of this study is to assess the environmental and human impacts of all Lancang-Mekong mainstem and tributary dams and the plans by many countries for more hydropower utilizing the potential of the river as the continent’s energy lifeline. Future dams need to include fish ladders and navigation locks to reduce the environmental impacts on fish populations, natural resources, navigation, and livelihoods. Strengthening of international collaboration via the MRC or by individual or multiple country agreements to address Lancang-Mekong’s sustainable transboundary development goals is recommended. When new Lancang-Mekong and tributary dams are built within any of the transboundary watershed countries, additional communities will need to be resettled. Significant environmental and human impacts are observed. Steps will have to be taken by all the concerned countries to prevent these problems and to ensure that people’s livelihoods are restored after resettlement.

ADAMS软件应用中解决数值发散的技巧

ＡＤＡＭＳ（机械系统自动动力分析）是世界上使用最广泛的机械系统仿真软件。分析了应用ＡＤＡＭＳ软件进行机械系统仿真分析过程中出现数值发散的原因，并提出了相应的解决技巧；从而极大限度地发挥ＡＤＡＭＳ软件的仿真功能。

博洛尼亚大学“DAMS”方向课程建构对我国高校音乐教育的启示

在中共中央办公厅、国务院办公厅印发《关于分类推进人才评价机制改革的指导意见》的通知(中办发[2018]6号)中,明确指出对人才应有"创新多元的评价方式"。笔者认为创新多元的评价方式首先需要有创新多元的教育理念和机制,通过对意大利博洛尼亚"DAMS"方向课程建构的深入剖析,以及我国高校音乐教育之比较,取长补短、洋为中用,能够更加清晰的明确国内高校音乐教育在未来几年的发展思路,在我国高校音乐教育的国际化进程中找到一些启示。

DAMS静力水准自动化监测系统在广州地铁中的实践

DAMS静力水准自动化监测系统是一种高精密液位测量系统,该系统适用于测量多点的相对沉降,具有精度高、自动化性能好等特点。主要用于大型水利枢纽工程等各测点不均匀沉降的测量。本文通过案例,介绍了该系统在广州地铁变形监测中的应用,可为类似的监测项目提供参考。

ON THE HYDRAULIC FAILURE PROBABILITY OF FLOOD CONTROL DAMS

In flood control dams it is not only the failure to prevent flood larger than their design carrying capacity, but also the uncertainties of hydraulic factors that cause disasters. In general, the hydraulic risk is not considered in most of the hydrological analysis in floodproofing plan and design. In this paper, a method of evaluating the hydraulic risk is developed by employing risk theory, and the concept can easily be extended to other types of risk analysis. As a result, it is possible not to consider the hydraulic resks when the design hydrologic risk of flood control dam is lger. Otherwise, the hydraulic risks must be noticed. The research is very helpful for the development of the flood control theory used at present.

基于DAMS的资产管理策略规划

根据电视传媒业发展的新情况 ,提出建立媒体资产管理系统的必要性 。

水体DAMS暴露对异育银鲫肝胰脏和鳃的氧化胁迫及组织学影响

探究了阴离子表面活性剂十二烷基苯磺酸异丙胺盐(DAMS)对异育银鲫(Carassiusauratusgibelio)肝胰脏和鳃组织的氧化胁迫和组织学的影响.异育银鲫在水体不同浓度的DAMS中暴露28d后,测定肝胰脏和鳃组织中丙二醛(MDA)含量、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-PX)活性以及观察肝胰脏和鳃组织的组织学切片.结果表明,DAMS对异育银鲫的96h半致死浓度LC50为19.83mg·L-1;在亚急性暴露实验中,随着毒物浓度升高,异育银鲫肝胰脏和鳃组织的GSH-PX和SOD活性均表现出显著的抑制(P<0.05),肝胰脏中MDA含量随暴露浓度的升高先上升后下降;高浓度暴露组鳃组织中MDA含量与对照组无显著差异,高浓度暴露组实验鱼的肝细胞出现明显的空泡化,鳃组织出现鳃上皮脱落等不同程度的损伤.结果暗示水体中的DAMS暴露对异育银鲫具有一定的氧化胁迫毒性.

Optimization design of foundation excavation for Xiluodu super-high arch dam in China

With better understanding of the quality and physico-mechanical properties of rocks of dam foundation,and the physico-mechanical properties and structure design of arch dam in association with the foundation excavation of Xiluodu arch dam,the excavation optimization design was proposed for the foundation surface on the basis of feasibility study.Common analysis and numerical analysis results demonstrated the feasibility of using the weakly weathered rocks III1and III2as the foundation surface of super-high arch dam.In view of changes in the geological conditions at the dam foundation along the riverbed direction,the design of extending foundation surface excavation area and using consolidating grouting and optimizing structure of dam bottom was introduced,allowing for harmonization of the arch dam and foundation.Three-dimensional(3D)geomechanics model test and fi nite element analysis results indicated that the dam body and foundation have good overload stability and high bearing capacity.The monitoring data showed that the behaviors of dam and foundation correspond with the designed patterns in the construction period and the initial operation period.

一种S波段雷达通信电子战多功能宽带DAM的设计

本文创新性地提出了一种S波段多功能宽带DAM,首先介绍了该型DAM的顶层架构设计,接着介绍了各个功能组成模块的设计过程,最后给出了该型DAM的指标实现情况。该型DAM可以分时实现雷达、通信及电子战功能,最大瞬时带宽可达200MHz,支持任意波形产生,在多功能综合射频系统中具有良好的应用前景。

Earthquake safety assessment of concrete arch and gravity dams

Based on research studies currently being carried out at Dalian University of Technology, some important aspects for the earthquake safety assessmcnt of concrete dams are reviewed and discussed. First, the rate-dependent behavior of concrcte subjected to earthquake loading is examined, emphasizing the properties of concrete under cyclic and biaxial loading conditions. Second, a modified four-parameter Hsieh-Ting-Chen viscoplastic consistency model is developed to simulate the rate-dependent behavior of concrete. The earthquake response of a 278m high arch dam is analyzed, and the results show that the strain-rate effects become noticeable in the inelastic range, Third, a more accurate non-smooth Newton algorithm for the solution of three-dimensional frictional contact problems is developed to study the joint opening effects of arch dams during strong earthquakes. Such effects on two nearly 300m high arch dams have been studied. It was found that the canyon shape has great influence on the magnitude and distribution of the joint opening along the dam axis. Fourth, the scaled boundary finite element method presented by Song and Wolf is employed to study the dam-reservoir-foundation interaction effects of concrete dams. Particular emphases were placed on the variation of foundation stiffness and the anisotropic behavior of the foundation material on the dynamic response of concrete dams. Finally, nonlinear modeling of concrete to study the damage evolution of concrete dams during strong earthquakes is discussed. An elastic-damage mechanics approach for damage prediction of concrete gravity dams is described as an example. These findings are helpful in understanding the dynamic behavior of concrete dams and promoting the improvement of seismic safety assessment methods.

Characteristics and factors that influenced damage to dams in the M_s 8.0 Wenchuan earthquake

Based on raw data from dams damaged in the Wenchuan earthquake, including many that were severely damaged, characteristics and factors that influenced the damage are discussed in this paper. Findings from this study include： severely damaged dams were densely distributed along the seismologic fault; small dams, especially small earth-rock dams, had the most serious damage that was caused by a variety of factors; the most serious damage was caused by seismic waves; damage was aggregated by aftershocks; and the extent of the damage patterns increased with the seismic intensity. Damage patterns varied in different intensity zones and cracking was the most common type of damage. Most of the dams had a good base with relatively high bearing capacity, and the walls of the earth-rock dams were mostly of clay soil. This type of base and body material mitigated some of the damage to dams. Reservoir maintenance and other factors also have a significant impact on the seismic safety of the dam. Finally, some recommendations to reduce seismic damage to dams are proposed.

Research on shape optimization of CSG dams

The multi-objective optimization method was used for shape optimization of cement sand and gravel （CSG） dams in this study. The economic efficiency, the sensitivities of maximum horizontal displacement and maximum settlement of the dam to water level changes, the overall stability, and the overall strength security were taken into account during the optimization process. Three weight coefficient selection schemes were adopted to conduct shape optimization of a dam, and the case studies lead to the conclusion that both the upstream-and downstream dam slope ratios for the optimal cross-section equal 1：0.7, which is consistent with the empirically observed range of 1：0.6 to 1;0.8 for the upstream and downstream dam slope ratios of CSG dams. Therefore, the present study is of certain reference value for designing CSG dams.

Key Technologies in the Design and Construction of 300 m Ultra-High Arch Dams

Starting with the Ertan arch dam （240 m high, 3300 MW） in 2000, China successfully built a total of seven ultra-high arch dams over 200 m tall by the end of 2014. Among these, the ]inping 1 （305 m）, Xiaowan （294.5m）, and Xiluodu （285.5 m） arch dams have reached the 300 m height level （i.e., near or over 300 m）, making them the tallest arch dams in the world. The design and construction of these 300 m ultra-high arch dams posed significant challenges, due to high water pressures, high seismic de- sign criteria, and complex geological conditions. The engineering team successfully tackled these chal- lenges and made critical breakthroughs, especially in the area of safety control. In this paper, the author summarizes various key technological aspects involved in the design and construction of 300 m ultra- high arch dams, including the strength and stability of foundation rock, excavation of the dam base and surface treatment, dam shape optimization, safety design guidelines, seismic analysis and design, treatment of a complex foundation, concrete temperature control, and crack prevention. The experience gained from these projects should be valuable for future practitioners.

Numerical simulation of seismic damage and cracking of concrete slabs of high concrete face rockfill dams

Based on the damage constitutive model for concrete, the Weibull distribution function was used to characterize the random distribution of the mechanical properties of materials by finely subdividing concrete slab elements, and a concrete random mesoscopic damage model was established. The seismic response of a 100-m high concrete face rockfill dam(CFRD), subjected to ground motion with different intensities, was simulated with the three-dimensional finite element method(FEM), with emphasis on exploration of damage and the cracking process of concrete slabs during earthquakes as well as analysis of dynamic damage and cracking characteristics during strong earthquakes. The calculated results show that the number of damaged and cracking elements on concrete slabs grows with the duration of earthquakes. With increasing earthquake intensity, the damaged zone and cracking zone on concrete slabs grow wider. During a 7.0-magnitude earthquake, the stress level of concrete slabs is low for the CFRD, and there is almost no damage or slight damage to the slabs. While during a 9.0-magnitude strong earthquake, the percentages of damaged elements and macrocracking elements continuously ascend with the duration of the earthquake, peaking at approximately 26% and 5% at the end of the earthquake, respectively. The concrete random mesoscopic damage model can depict the entire process of sprouting, growing, connecting, and expanding of cracks on a concrete slab during earthquakes.

Effects of river flow velocity on the formation of landslide dams

Natural dams are formed when landslides are triggered by heavy rainfall during extreme weather events in the mountainous areas of Taiwan.During landslide debris movement,two processes occur simultaneously:the movement of landslide debris from a slope onto the riverbed and the erosion of the debris under the action of high-velocity river flow.When the rate of landslide deposition in a river channel is higher than the rate of landslide debris erosion by the river flow,the landslide forms a natural dam by blocking the river channel.In this study,the effects of the rates of river flow erosion and landslide deposition(termed the erosive capacity and depositional capacity,respectively)on the formation of natural dams are quantified using a physics-based approach and are tested using a scaled physical model.We define a dimensionless velocity index vde as the ratio between the depositional capacity of landslide debris(vd)and the erosive capacity of water flow(ve).The experimental test results show that a landslide dam forms when landslide debris moves at high velocity into a river channel where the river-flow velocity is low,that is,the dimensionless velocity index vde>54.Landslide debris will not have sufficient depositional capacity to block stream flow when the dimensionless velocity index vde<47.The depositional capacity of a landslide can be determined from the slope angle and the friction of the sliding surface,while the erosive capacity of a dam can be determined using river flow velocity and rainfall conditions.The methodology described in this paper was applied to seven landslide dams that formed in Taiwan on 8 August 2009 during Typhoon Morakot,the Tangjiashan landslide dam case,and the Yingxiu-Wolong highway K24 landslide case.The dimensionless velocity index presented in this paper can be used before a rainstorm event occurs to determine if the formation of a landslide dam is possible.

Seismic responses of high concrete face rockfill dams:A case study

Seismic responses of the Zipingpu concrete face rockfill dam were analyzed using the finite element method. The dynamic behavior of rockfill materials was modeled with a viscoelastic model and an empirical permanent strain model. The relevant parameters were obtained either by back analysis using the field observations or by reference to parameters of similar rockfill materials. The acceleration responses of the dam,the distribution of earthquake-induced settlement, and the gap propagation under the concrete slabs caused by the settlement of the dam were analyzed and compared with site investigations or relevant studies. The mechanism of failure of horizontal construction joints was also analyzed based on numerical results and site observations. Numerical results show that the input accelerations were considerably amplified near the top of the dam, and the strong shaking resulted in considerable settlement of the rockfill materials, with a maximum value exceeding 90 cm at the crest.As a result of the settlement of rockfill materials, the third-stage concrete slabs were separated from the cushion layer. The rotation of the cantilever slabs about the contacting regions, under the combined action of gravity and seismic inertial forces, led to the failure of the construction joints and tensile cracks appeared above the construction joints. The effectiveness and limitations of the so-called equivalent linear method are also discussed.

Reducing risks in the investigation, design and construction of large concrete dams

An overview of the GeoSafe 2016 Symposium topic is provided using the example of large concrete dams for purposes of illustration.It is essential that the risks associated with large dams be evaluated rigorously and managed proactively at all stages of their lives so that the risk of failure remains As Low As Reasonably Practicable(ALARP).Rock engineering features of large concrete dams that require particular attention,assessment and monitoring during the investigation,design,construction,initial filling,inservice operation,and subsequent repair and upgrade stages of the lives of concrete dams are identified and illustrated by examples from recorded experiences.A number of major concrete dam failures,including that of the St.Francis dam,California,U.S.A.,in 1928,have led to significant developments in rock mechanics and rock engineering knowledge and techniques,as well as in dam design and review processes.More recent advances include a range of analytical,numerical modelling,probabilistic,reliability,failure mode and risk assessment approaches.