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Regulation of superoxide flashes by transient and steady mitochondrial calcium elevations 被引量:5
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作者 JIAN ChongShu HOU TingTing +2 位作者 YIN RongKang CHENG HePing WANG XianHua 《Science China(Life Sciences)》 SCIE CAS 2014年第5期495-501,共7页
The mitochondria play essential roles in both intracellular calcium and reactive oxygen species signaling.As a newly discovered universal and fundamental mitochondrial phenomenon,superoxide flashes reflect transient b... The mitochondria play essential roles in both intracellular calcium and reactive oxygen species signaling.As a newly discovered universal and fundamental mitochondrial phenomenon,superoxide flashes reflect transient bursts of superoxide production in the matrix of single mitochondria.Whether and how the superoxide flash activity is regulated by mitochondrial calcium remain largely unknown.Here we demonstrate that elevating mitochondrial calcium either by the calcium ionophore ionomycin or by increasing the bathing calcium in permeabilized HeLa cells increases superoxide flash incidence,and inhibition of the mitochondrial calcium uniporter activity abolishes the flash response.Quantitatively,the superoxide flash incidence is correlated to the steady-state mitochondrial calcium elevation with 1.7-fold increase per 1.0?F/F0 of Rhod-2 signal.In contrast,large mitochondrial calcium transients(e.g.,peak△F/F0~2.8,duration^2 min)in the absence of steady-state elevations failed to alter the flash activity.These results indicate that physiological levels of sustained,but not transient,mitochondrial calcium elevation acts as a potent regulator of superoxide flashes,but its mechanism of action likely involves a multi-step,slow-onset process. 展开更多
关键词 线粒体 钙调节 稳态 超氧 闪烁 瞬态 细胞内钙离子 HELA细胞
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线粒体稳定高钙信号诱发超氧炫 被引量:1
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作者 简崇书 侯婷婷 +2 位作者 殷荣康 程和平 王显花 《中国科学:生命科学》 CSCD 北大核心 2013年第10期869-876,共8页
线粒体对于细胞钙信号和活性氧信号转导有重要的调控作用.超氧炫是新近发现的单个线粒体超氧阴离子短时程爆发现象,反映了活性氧生成动力学的一种新形式.线粒体钙信号作为重要的细胞功能调控信号,能否及如何调控超氧炫尚待深入研究.本... 线粒体对于细胞钙信号和活性氧信号转导有重要的调控作用.超氧炫是新近发现的单个线粒体超氧阴离子短时程爆发现象,反映了活性氧生成动力学的一种新形式.线粒体钙信号作为重要的细胞功能调控信号,能否及如何调控超氧炫尚待深入研究.本研究对HeLa细胞进行高胞外钙和离子霉素刺激,或用皂苷穿孔细胞质膜后置于高钙细胞内液中,两种方法均显著增加了超氧炫发生的频率.其中,穿孔细胞胞浆高钙诱导的超氧炫依赖于线粒体钙单向转运体,表明超氧炫由线粒体基质内高钙信号所诱发.重要的是,离子霉素诱导的超氧炫发生频率与线粒体稳态钙水平线性相关,而与瞬态线粒体钙无相关性,提示钙离子对超氧炫的调控是一个多步骤、相对缓慢的过程.综上,线粒体基质的稳态高钙是超氧炫的重要调控因子. 展开更多
关键词 超氧炫 钙信号 活性氧 线粒体
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