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From Control to Empowerment:A Paradigm Shift in the Discourse of Educational Supervision
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作者 Jinhua Zhou Kai Zhang 《Journal of Contemporary Educational Research》 2024年第9期176-180,共5页
As educational reforms intensify and societal emphasis shifts towards empowerment,the traditional discourse paradigm of management and control in educational supervision faces growing challenges.This paper explores th... As educational reforms intensify and societal emphasis shifts towards empowerment,the traditional discourse paradigm of management and control in educational supervision faces growing challenges.This paper explores the transformation of this discourse paradigm through the lens of empowerment,analyzing its distinct characteristics,potential pathways,and effective strategies.This paper begins by reviewing the concept of empowerment and examining the current research landscape surrounding the discourse paradigm in educational supervision.Subsequently,we conduct a comparative analysis of the“control”and“empowerment”paradigms,highlighting their essential differences.This analysis illuminates the key characteristics of an empowerment-oriented approach to educational supervision,particularly its emphasis on dialogue,collaboration,participation,and,crucially,empowerment itself.Ultimately,this research advocates for a shift in educational supervision towards an empowerment-oriented discourse system.This entails a multi-pronged approach:transforming ingrained beliefs,embracing renewed pedagogical concepts,fostering methodological innovation,and optimizing existing mechanisms and strategies within educational supervision.These changes are proposed to facilitate the more effective alignment of educational supervision with the pursuit of high-quality education. 展开更多
关键词 Educational supervision Paradigm shift CONTROL EMPOWERMENT
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Study on Independent Supervision Function of Design Assurance System
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作者 SUI Lijun MA Congyao WANG Jianyuan 《International Journal of Plant Engineering and Management》 2024年第3期185-192,共8页
The effective operation of a design assurance system cannot be achieved without the effective performance of the independent supervision function.As one of the core functions of the design assurance system,the purpose... The effective operation of a design assurance system cannot be achieved without the effective performance of the independent supervision function.As one of the core functions of the design assurance system,the purpose of the independent supervision function is to ensure that the system operates within the scope of procedures and manuals.At present,the function of independent supervision is a difficult and confusing issue for various original equipment manufacturers as well as suppliers,and there is an urgent requirement to put forward relevant requirements and form relevant methods.Based on the above mentioned objective,the basic requirements of the independent supervision function of design assurance system were studied,the problems and deficiencies in the organization,staffing,and methods existing in the current independent supervision function were analyzed,the improvement suggestions and measures for the performance of the independent supervision function from the aspects of the organization,staffing,procedures,and suppliers were put forward.The present work and conclusions provide guidance and direction for the effective operation of the design assurance system. 展开更多
关键词 design of assurance systems independent supervision functions system operation internal audits SUPPLIERS
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Supervision of Public Opinion under the Background of Social Co-governance-Take the Changsheng Vaccine Incident as an Example
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作者 Ma Xiaonan Tian Lijuan 《Asian Journal of Social Pharmacy》 2023年第3期198-205,共8页
Objective To provide reference for the news media to give play to the role of public opinion supervision in time based on the background of drug safety and social co-governance.Methods The method of case analysis was ... Objective To provide reference for the news media to give play to the role of public opinion supervision in time based on the background of drug safety and social co-governance.Methods The method of case analysis was used to make a retrospective study on the Changsheng vaccine incident in 2018.Then the role of mainstream media,pharmaceutical media,and self-media in the supervision of public opinion was investigated.Results and Conclusion Both mainstream and pharmaceutical media played an excellent role in supervising the Changchun Changsheng vaccine incident.However,the content published by some pharmaceutical media was hard to understand by ordinary people.Besides,the role of self-media in public opinion supervision was polarized.Some self-media closely kept pace with mainstream media in public opinion supervision.Other self-media unilaterally pursued the click rate,publishing false information to guide wrong public opinion.The news media should optimize the supervision efficiency of drug safety.On the one hand,pharmaceutical media should pay attention to the fact that readers may not understand the difficult terms because they are not professional.On the other hand,self-media practitioners should improve their professional quality so that they will not publish some fake news to mislead public opinion. 展开更多
关键词 social co-governance Changchun Changsheng vaccine incident news media public opinion supervision
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人参皂苷Rc通过调控AMPK通路介导的细胞焦亡减轻小鼠脑缺血再灌注损伤
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作者 李良勇 马莉 +1 位作者 李亚军 梅灯 《中国病理生理杂志》 CAS CSCD 北大核心 2024年第5期844-851,共8页
目的:探讨人参皂苷Rc对脑缺血再灌注损伤(CIRI)小鼠的保护作用,并从细胞焦亡角度阐释其作用机制。方法:将C57BL/6小鼠随机分为假手术(sham)组、大脑中动脉闭塞/再灌注(MCAO/R)组、人参皂苷Rc+MCAO/R组(Rc组)、AMP活化蛋白激酶(AMPK)激... 目的:探讨人参皂苷Rc对脑缺血再灌注损伤(CIRI)小鼠的保护作用,并从细胞焦亡角度阐释其作用机制。方法:将C57BL/6小鼠随机分为假手术(sham)组、大脑中动脉闭塞/再灌注(MCAO/R)组、人参皂苷Rc+MCAO/R组(Rc组)、AMP活化蛋白激酶(AMPK)激动剂阿卡地新(AICAR)+MCAO/R组(agonist组)和人参皂苷Rc+MCAO/R+AMPK抑制剂Compound C组(Rc+inhibitor组)。对agonist组小鼠给予AICAR(500 mg/kg)腹腔注射,Rc+inhibitor组小鼠给予Compound C(20 mg/kg)腹腔注射;Rc组和Rc+inhibitor组小鼠造模后给予人参皂苷Rc(40 mg/kg)灌胃,每天一次,连续7 d;sham组和MCAO/R组则给予等体积的纯化水。Zea Longa评分观察小鼠神经功能缺损程度,TTC染色观察小鼠脑梗死体积,干湿重法观察小鼠脑水肿程度,HE染色观察小鼠脑组织病理形态的改变,RT-qPCR和Western blot检测小鼠脑组织AMPK、核苷酸结合寡聚化结构域样受体蛋白3(NLRP3)、胱天蛋白酶1(caspase-1)和焦亡效应蛋白消皮素D(GSDMD)的表达,ELISA检测炎症因子白细胞介素1β(IL-1β)和IL-18的含量。结果:Rc组和agonist组小鼠神经功能缺损症状减轻,脑梗死体积减小,脑水肿和神经元病理损伤受到抑制,脑组织pAMPK/AMPK比值和AMPK mRNA表达水平升高,细胞焦亡相关蛋白NLRP3、caspase-1和GSDMD的mRNA和蛋白表达水平降低,炎症因子IL-1β和IL-18表达水平降低。Rc+inhibitor组小鼠脑组织p-AMPK/AMPK比值和AMPK mRNA表达水平较Rc组显著下降(P<0.05),NLRP3、caspase-1和GSDMD的mRNA和蛋白表达水平,以及IL-1β和IL-18表达水平显著升高(P<0.05),小鼠神经功能缺损程度评分升高,脑梗死体积增大,脑水肿和神经元病理损伤显著加重(P<0.05)。结论:人参皂苷Rc可能通过活化AMPK通路抑制NLRP3/caspase-1/GSDMD介导的细胞焦亡,从而减轻小鼠CIRI。 展开更多
关键词 人参皂苷Rc 脑缺血再灌注损伤 amp活化蛋白激酶 细胞焦亡
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黄芩苷调节cAMP/PKA/CREB信号通路对湿疹大鼠皮肤屏障功能的影响 被引量:3
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作者 王首帆 徐宜厚 +1 位作者 徐爱琴 朱立宏 《天津医药》 CAS 2024年第2期148-153,共6页
目的探讨黄芩苷(BA)调节环磷酸腺苷(cAMP)/蛋白激酶A(PKA)/cAMP反应元件结合蛋白(CREB)通路对湿疹大鼠皮肤屏障功能的影响。方法将SD大鼠随机分为对照组(NC组)、Model组、低剂量BA组(BA-L组,25 mg/kg)、中剂量BA组(BA-M组,50 mg/kg)、... 目的探讨黄芩苷(BA)调节环磷酸腺苷(cAMP)/蛋白激酶A(PKA)/cAMP反应元件结合蛋白(CREB)通路对湿疹大鼠皮肤屏障功能的影响。方法将SD大鼠随机分为对照组(NC组)、Model组、低剂量BA组(BA-L组,25 mg/kg)、中剂量BA组(BA-M组,50 mg/kg)、高剂量BA组(BA-H组,100 mg/kg)、泼尼松组(PNS组,25 mg/kg)、BAH+cAMP抑制剂(SQ22536)组(100 mg/kg+2.13 mg/kg)、BA-H+PKA抑制剂(H-89)组(100 mg/kg+5 mg/kg),每组12只。除NC组外,其余组大鼠均构建湿疹大鼠模型。建模成功2 d后,分组进行给药处理。检测湿疹面积及严重度指数(EASI)评分、经皮肤水分流失量(TEWL)、角质层含水量(WCSC)变化;酶联免疫吸附试验(ELISA)检测大鼠血清中免疫球蛋白E(IgE)、干扰素-γ(IFN-γ)、白细胞介素-4(IL-4)水平及大鼠背部受试区皮损组织中c AMP蛋白表达;HE染色检测大鼠背部受试区皮损组织病理变化;Western blot检测大鼠背部受试区皮损组织中水通道蛋白3(AQP3)、cathelicidin相关抗菌肽(CRAMP)、p-PKA、p-CREB蛋白表达。结果与NC组比较,Model组大鼠背部受试区皮损组织病理损伤严重,EASI评分、TEWL、IgE、IL-4水平升高,WCSC、IFN-γ水平、AQP3、CRAMP、cAMP、p-PKA、p-CREB蛋白水平降低(P<0.05)。与Model组比较,BA-L组、BA-M组、BA-H组、PNS组大鼠背部受试区皮损组织病理损伤减轻,EASI评分、TEWL、IgE、IL-4水平降低,WCSC、IFN-γ水平、AQP3、CRAMP、cAMP、p-PKA、p-CREB蛋白水平升高(P<0.05);且BA-L组、BA-M组、BA-H组上述指标变化呈剂量依赖性。SQ22536或H-89减弱了高剂量BA对湿疹大鼠皮肤屏障功能的改善作用。结论BA可能通过激活cAMP/PKA/CREB信号通路改善湿疹大鼠皮肤屏障功能。 展开更多
关键词 湿疹 黄芩苷 环磷酸腺苷/蛋白激酶A/camp反应元件结合蛋白通路 皮肤屏障功能
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基于NOD2介导的AMPK/mTOR信号通路探讨宫颈癌细胞恶性行为的机制
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作者 杜瑞亭 伍东月 +1 位作者 郭清民 靳冬梅 《安徽医科大学学报》 CAS 北大核心 2024年第2期316-324,共9页
目的 基于核苷酸结合寡聚化结构域受体2(NOD2)介导的AMP活化蛋白激酶(AMPK)/雷帕霉素靶蛋白(mTOR)信号通路探讨宫颈癌(CC)细胞恶性行为的机制。方法 生物信息学分析确定NOD2在CC组织中的表达。将靶向NOD2(shNOD2)、shRNAs阴性对照(shNC... 目的 基于核苷酸结合寡聚化结构域受体2(NOD2)介导的AMP活化蛋白激酶(AMPK)/雷帕霉素靶蛋白(mTOR)信号通路探讨宫颈癌(CC)细胞恶性行为的机制。方法 生物信息学分析确定NOD2在CC组织中的表达。将靶向NOD2(shNOD2)、shRNAs阴性对照(shNC)以及NOD2过表达(NOD2)质粒和载体(Vec)转染CC细胞。通过CCK-8测定、集落形成和Transwell细胞侵袭测定来确定NOD2对CC细胞生长的影响。通过高通量RNA测序(RNA-Seq)进行转录组分析。Western blot试验检测细胞系中NOD2、AMPK/mTOR信号通路和自噬蛋白的表达。24只雌性BALB/c裸鼠随机分为4组,每组6只:载体组(Vec组)、NOD2过表达组(NOD2组)、shNC组和shNOD2组。构建小鼠远处转移模型,监测肺转移的荧光强度,计数肺转移结节的数量。结果 在线数据库分析显示,NOD2在CC组织中表达明显高于正常组织,并且不同分期的CC中NOD2的mRNA表达差异有统计学意义(P<0.05)。此外,NOD2的高表达与较差的总生存期和无病生存期相关(P<0.05)。NOD2过表达对CC细胞增殖、集落形成、迁移和侵袭具有促进作用,而NOD2敲低则相反。与体外结果一致,在转移的小鼠尾静脉注射模型中,NOD2组CC细胞的肺定殖、肺转移灶较Vec组增加(P<0.05),而shNOD2组CC细胞的肺定殖、肺转移灶较shNC组减少(P<0.05)。RNA-Seq结果显示NOD2表达与AMPK信号激活、mTOR信号抑制、自噬调节途径激活和自噬体形成显著相关。与shNC组相比,shNOD2组磷酸化AMPK、LC3蛋白表达水平减少(P<0.05),磷酸化mTOR、p62蛋白表达水平增加(P<0.05);与Vec组相比,NOD2组LC3、AMPK蛋白表达水平增加(P<0.05),磷酸化mTOR、p62蛋白表达水平减少(P<0.05)。与shNC组相比,shNOD2组GFP-mRFP-LC3的点积累减少(P<0.05);与Vec组相比,GFP-mRFP-LC3的点积累增加(P<0.05)。结论 NOD2可能通过AMPK/mTOR信号促进CC增殖、迁移和侵袭,其作用机制部分涉及自噬激活。 展开更多
关键词 核苷酸结合寡聚化结构域受体2 amp活化蛋白激酶 雷帕霉素靶蛋白 宫颈癌细胞 自噬
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川陈皮素调节AMPK/NLRP3信号通路对脂多糖诱导的肾小球系膜细胞炎性损伤的影响
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作者 罗丹 王燕 +1 位作者 丁旭 胡耀 《中药新药与临床药理》 CAS CSCD 北大核心 2024年第2期224-229,共6页
目的探讨川陈皮素(Nobiletin)调节AMP激活的蛋白激酶(AMPK)/NOD样受体蛋白3(NLRP3)信号通路对脂多糖(LPS)诱导的肾小球系膜细胞HBZY-1炎性损伤的影响。方法将HBZY-1细胞分为5组:正常组、LPS组(100 ng·m L^(-1)LPS)、川陈皮素组(100... 目的探讨川陈皮素(Nobiletin)调节AMP激活的蛋白激酶(AMPK)/NOD样受体蛋白3(NLRP3)信号通路对脂多糖(LPS)诱导的肾小球系膜细胞HBZY-1炎性损伤的影响。方法将HBZY-1细胞分为5组:正常组、LPS组(100 ng·m L^(-1)LPS)、川陈皮素组(100 ng·m L^(-1)LPS+40μmol·L^(-1)川陈皮素)、AMPK/NLRP3信号通路抑制剂雷帕霉素组(100 ng·m L^(-1)LPS+0.5μmol·L^(-1)雷帕霉素)、川陈皮素+雷帕霉素组(100 ng·m L^(-1)LPS+40μmol·L^(-1)川陈皮素+0.5μmol·L^(-1)雷帕霉素)。MTT法检测HBZY-1细胞毒性和增殖;ELISA法检测HBZY-1细胞白细胞介素(IL)-1β、IL-6、肿瘤坏死因子α(TNF-α)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、谷胱甘肽(GSH)含量;流式细胞术检测细胞凋亡;Western Blot法检测AMPK/NLRP3信号通路蛋白水平。结果与正常组比较,LPS组CAT、SOD、GSH水平、细胞OD值以及AMPK蛋白水平明显降低(P<0.05),细胞凋亡率、IL-1β、IL-6、TNF-α含量以及NLRP3蛋白水平明显升高(P<0.05)。与LPS组比较,川陈皮素组CAT、SOD、GSH水平、OD值以及AMPK蛋白水平明显升高(P<0.05),细胞凋亡率、IL-1β、IL-6、TNF-α含量以及NLRP3蛋白水平明显下降(P<0.05);而雷帕霉素组以上指标均呈现与川陈皮素组相反的趋势(P<0.05)。与川陈皮素组比较,川陈皮素+雷帕霉素组以上指标均呈现与川陈皮素组相反的趋势(P<0.05)。结论川陈皮素可能通过上调AMPK/NLRP3信号通路减轻LPS诱导的肾小球系膜细胞细胞炎性损伤。下调AMPK/NLRP3信号通路可消除川陈皮素对LPS诱导的肾小球系膜细胞细胞炎性损伤的改善作用。 展开更多
关键词 川陈皮素 amp激活的蛋白激酶/NOD样受体蛋白3(ampK/NLRP3)信号通路 脂多糖 肾小球系膜细胞 HBZY-1 炎性损伤
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基于AMPK/sirt1/RANKL/OPG通路研究补阳还五汤促进骨质疏松性骨折大鼠愈合的机制 被引量:1
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作者 林玉玲 李杰玉 +3 位作者 李增一 刘琳 籍胤玺 金毅 《中国老年学杂志》 CAS 北大核心 2024年第1期133-137,共5页
目的基于AMP活化蛋白激酶(AMPK)/沉默信息调节因子(sirt)1/核转录因子(NF)-κB受体活化蛋白配体(RANKL)/骨保护素(OPG)信号通路,探究补阳还五汤促进骨质疏松性骨折大鼠骨折愈合的机制。方法将大鼠随机分成假手术组、模型组、补阳还五汤... 目的基于AMP活化蛋白激酶(AMPK)/沉默信息调节因子(sirt)1/核转录因子(NF)-κB受体活化蛋白配体(RANKL)/骨保护素(OPG)信号通路,探究补阳还五汤促进骨质疏松性骨折大鼠骨折愈合的机制。方法将大鼠随机分成假手术组、模型组、补阳还五汤低剂量组(6.25 g/kg)、补阳还五汤中剂量组(12.50 g/kg)和补阳还五汤高剂量组(25.00 g/kg)。除假手术组外,其余组均构建骨质疏松性骨折模型,并给予相应剂量药物干预。检测大鼠骨密度(BMD)值、骨折愈合评分及骨痂体积;自动生化分析仪测定血清碱性磷酸酶(ALP)、骨钙素、钙和磷水平;酶联免疫吸附试验(ELISA)检测股骨组织中的丙二醛(MDA)、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)水平;苏木素-伊红(HE)染色观察各组大鼠骨痂组织的病理形态;Western印迹检测股骨组织中AMPK/sirt1/RANKL/OPG通路蛋白表达。结果与假手术组相比,模型组骨痂骨小梁分布稀疏,成骨细胞大量减少且有纤维组织生成,骨痂体积、血清中ALP、骨钙素、钙和磷水平及股骨组织中MDA水平、RANKL蛋白水平显著升高(P<0.05),BMD值、骨折愈合评分、骨组织中SOD和GSH-Px水平和AMPK、sirt1、OPG蛋白水平显著降低(P<0.05)。与模型组相比,补阳还五汤低、中、高剂量组骨痂骨小梁分布较为密集,成骨细胞增加,骨痂体积、血清中ALP、骨钙素、钙和磷水平及股骨组织中MDA水平、RANKL蛋白水平显著降低(P<0.05),BMD值、骨折愈合评分、骨组织中SOD和GSH-Px水平和AMPK、sirt1、OPG蛋白水平显著升高(P<0.05)。结论补阳还五汤可能通过抑制氧化应激反应,调节AMPK/sirt1/RANKL/OPG通路,发挥对骨质疏松骨折大鼠的治疗作用。 展开更多
关键词 补阳还五汤 骨质疏松性骨折 氧化应激 amp活化蛋白激酶(ampK)/沉默信息调节因子(sirt)1/核转录因子(NF)-κB受体活化蛋白配体(RANKL)/骨保护素(OPG)通路
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基于AMPK通路探讨茶多酚改善糖尿病大鼠糖脂代谢机制
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作者 黄苏萍 林欢 +2 位作者 曾淑华 杨柳媛 许陆达 《山东中医药大学学报》 2024年第2期192-200,共9页
目的:探讨茶多酚从AMP活化蛋白激酶(AMPK)通路途径调控糖尿病大鼠糖脂代谢的机制。方法:经糖耐量试验筛选6周龄SD大鼠45只,按随机数字表法分为正常组10只和造模组35只,造模组糖尿病造模成功后剩余30只,根据体质量分层法和血糖水平分为... 目的:探讨茶多酚从AMP活化蛋白激酶(AMPK)通路途径调控糖尿病大鼠糖脂代谢的机制。方法:经糖耐量试验筛选6周龄SD大鼠45只,按随机数字表法分为正常组10只和造模组35只,造模组糖尿病造模成功后剩余30只,根据体质量分层法和血糖水平分为模型组、二甲双胍组和茶多酚组,每组各10只。各组均于每日下午灌胃,正常组与模型组给予无菌生理盐水,二甲双胍组给予二甲双胍水溶液,茶多酚组给予茶多酚。干预120 d后,检测各组大鼠的空腹血糖(FBG)、总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)和低密度脂蛋白胆固醇(LDL-C);酶联免疫吸附试验法检测肝脏乙酰辅酶A羧化酶(ACC)和激素敏感性脂肪酶(HSL);实时荧光定量法检测肝脏ACCmRNA、转录因子胆固醇调节元件结合蛋白1(SREBP-1)mRNA、3-羟基-3-甲基戊二酸单酰辅酶A还原酶(HMGCR)mRNA;免疫印迹法检测肝脏AMPKα1、磷酸化AMP活化蛋白激酶α1(P-AMPKα1)、SREBP1、HMGCR、肉毒碱棕榈酰转移酶1(CPT1)、葡萄糖转运蛋白4(GLUT4)和磷酸烯醇丙酮酸羧化激酶(PEPCK)。结果:与正常组比较,模型组大鼠的一般情况较差,FBG、TG、TC、LDL-C、ACC升高(P<0.05),SREBP1、HMGCR蛋白及mRNA表达升高(P<0.05),CPTI、PEPCK蛋白表达升高(P<0.05),HDL-C、HSL降低(P<0.05),AMPKα1、P-AMPKα1、GLUT4蛋白表达降低(P<0.05)。与模型组比较,茶多酚组大鼠的一般情况改善,TG、LDL-C、ACC降低(P<0.05),SREBP1、HMGCR蛋白及mRNA表达降低(P<0.05),PEPCK蛋白表达降低(P<0.05),HDL-C、HSL升高(P<0.05),AMPKα1、P-AMPKα1、GLUT4、CPT1蛋白表达升高(P<0.05)。与二甲双胍组比较,茶多酚组TC升高(P<0.05),FBG、TG、LDL-C、HDL-C、HSL、ACC、ACC mRNA、HMGCR mRNA、SREBP1 mRNA、AMPKα1、P-AMPKα1、SREBP1、HMGCR、CPT1、GLUT4、PEPCK相对表达量差异无统计学意义(P>0.05)。结论:茶多酚可能通过调节AMPK通路,影响HSL、HMGCR、SREBP1、CPTI、GLUT4和PEPCK,从而改善糖尿病大鼠糖脂代谢。 展开更多
关键词 糖尿病 茶多酚 二甲双胍 amp活化蛋白激酶通路 糖脂代谢 大鼠
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阿江榄仁酸由AMPK/mTOR/HO-1信号通路调控自噬对糖尿病视网膜病变影响
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作者 蒋晨 万新娟 +2 位作者 王绍飞 王晓虹 丁琳 《河北医药》 CAS 2024年第2期171-175,共5页
目的探讨阿江榄仁酸(arjunolic acid,AA)对糖尿病视网膜病变(diabetic retinopathy,DR)大鼠视网膜细胞自噬及AMPK/mTOR/HO-1信号通路的影响。方法以健康SD大鼠为研究对象,构建链脲佐菌素(STZ)诱导的糖尿病大鼠模型,随机分为对照组(Con)... 目的探讨阿江榄仁酸(arjunolic acid,AA)对糖尿病视网膜病变(diabetic retinopathy,DR)大鼠视网膜细胞自噬及AMPK/mTOR/HO-1信号通路的影响。方法以健康SD大鼠为研究对象,构建链脲佐菌素(STZ)诱导的糖尿病大鼠模型,随机分为对照组(Con)组、模型(STZ)组、AA低剂量(AAL,10 mg/kg)组和AA高剂量(AAH,10 mg/kg)组。连续给药10周后,HE染色检测视网膜组织病理结构;qRT-PCR检测视网膜组织白介素(IL)-1β、IL-6和线粒体丙酮酸转运载体(MPC)-1的mRNA表达;二氢乙锭(DHE)染色评估视网膜组织ROS产生;Western blot检测自噬和AMPK/mTOR/HO-1信号通路相关蛋白表达。结果与Con组比较,STZ组大鼠视网膜出现肿胀和空泡样变化等病理变化,中央视网膜ONL层厚度和细胞核计数明显降低(P<0.01);IL-1β、IL-6和MCP-1的mRNA水平显著增高(P<0.05);视网膜外核层(ONL)、内核层(INL)和神经节细胞层(GCL)中ROS产生增加(P<0.01);LC3II/I比率、HO-1和p-AMPK/AMPK蛋白表达显著降低,p62和p-mTOR/mTOR表达升高(P<0.01)。与STZ组比较,AAL和AAH组大鼠视网膜ONL厚度和细胞核计数逐渐升高,结构相对规整(P<0.05);AAH组IL-1β、IL-6和MCP-1的mRNA表达明显降低(P<0.05);视网膜ONL、INL和GCL中ROS产生逐渐降低(P<0.01);LC3II/I比率、p-AMPK/AMPK和HO-1表达逐渐升高,p62和p-mTOR/mTOR表达逐渐降低(P<0.01)。结论阿江榄仁酸可能是治疗DR的候选药物,可能机制为通过AMPK/mTOR/HO-1调节的自噬途径保护视网膜细胞免受STZ诱导的氧化应激和炎症损伤。 展开更多
关键词 阿江榄仁酸 糖尿病视网膜病变 ampK/mTOR/HO-1通路 自噬
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Foodborne doping and supervision in sports 被引量:1
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作者 Wei Chen Xiaoyu Cheng +1 位作者 Yingnan Ma Ning Chen 《Food Science and Human Wellness》 SCIE CSCD 2023年第6期1925-1936,共12页
Cases of foodborne doping are frequently reported in sports events and can cause severe consequences for athletes.The foodborne doping can be divided into natural endogenous and artifi cially added foods according to ... Cases of foodborne doping are frequently reported in sports events and can cause severe consequences for athletes.The foodborne doping can be divided into natural endogenous and artifi cially added foods according to the sources,including anabolic agents,stimulants,diuretics,β-blockers,β2 agonists and others.In order to control foodborne doping,chromatographic technique,immunoassay,nuclear magnetic resonance,biosensor technology,pyrolytic spectroscopy,comprehensive analysis and electrochemical analysis have usually used as analytical and inspection strategies.Meanwhile,the legislation of anti-doping,the improvement of testing standard and technology,and the prevention and control of food safety,as well as the improvement of risk perception of athletes are highly necessary for achieving the effective risk control and supervision of foodborne doping,which will be benefi cial for athletes,doctors and administrators to avoid the risks of foodborne doping test and reduce foodborne doping risks for the health of athletes. 展开更多
关键词 Foodborne doping Doping control ATHLETES ANTI-DOPING supervision
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Research on the intelligent supervision and operation platform of railway real estate 被引量:2
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作者 Xiangru Lv Hui Li Beisheng Liu 《High-Speed Railway》 2023年第2期147-152,共6页
Railway real estate is the fundamental element of railway transportation production and operation.Effective management and rational utilization of railway real estate is essential for railway asset operation.Based on ... Railway real estate is the fundamental element of railway transportation production and operation.Effective management and rational utilization of railway real estate is essential for railway asset operation.Based on the investigation of the requirements of railway real estate management and operation,combined with Beidou positioning,GIS(Geographic Information System),multi-source data fusion and other cutting-edge technologies,this paper puts forward the multi-dimensional dynamic statistical method of real estate information,the identification method of railway land occupation and the comprehensive evaluation method of real estate development and utilization potential,and build the railway real estate supervision and operation platform,design the function of the platform,so as to provide intelligent solutions for the railway real estate operation. 展开更多
关键词 RAILWAY Real estate supervision GIS Beidou positioning Multi-sourcedata fusion
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超高矿化度AM/AMPS共聚物压裂液与交联过程流变学
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作者 田珍瑞 方波 +2 位作者 辛慧 卢拥军 许可 《应用化工》 CAS CSCD 北大核心 2024年第5期1086-1092,共7页
对不同矿化度、不同盐溶液中AM/AMPS压裂液流变性能及其在超高矿化度时的交联凝胶状态与流变学性质以及交联过程进行了研究。结果表明,AM/AMPS压裂液KCl与CaCl_(2)溶液中黏度与弹性模量下降,且CaCl_(2)的影响更显著;而超高矿化度镁盐溶... 对不同矿化度、不同盐溶液中AM/AMPS压裂液流变性能及其在超高矿化度时的交联凝胶状态与流变学性质以及交联过程进行了研究。结果表明,AM/AMPS压裂液KCl与CaCl_(2)溶液中黏度与弹性模量下降,且CaCl_(2)的影响更显著;而超高矿化度镁盐溶液对压裂液黏度与黏弹性有增强作用。在超高矿化度的不同盐溶液中,AM/AMPS均可以与有机锆交联剂形成能完全挑挂凝胶,应变扫描结果证明,交联后压裂液表现出典型的凝胶特征。AM/AMPS压裂液在高矿化度盐溶液以及水溶液中的30℃恒温小振幅振荡交联过程可用四参数交联流变动力学方程描述,金属盐离子可以提高凝胶强度,且二价盐的提高更为显著。AM/AMPS压裂液升温小振幅振荡交联过程存在缓速交联与快速交联两个阶段,适当升温有利于交联反应。AM/AMPS压裂液在自来水与一价盐溶液中升温大振幅振荡交联过程与升温小振幅振荡交联过程相似;在二价盐溶液中交联过程受剪切影响较大,适当提高剪切,有利于AM/AMPS压裂液在盐溶液中交联。研究获得了超高矿化度AM/AMPS压裂液新体系,有望为AM/AMPS压裂液工程应用提供指导。 展开更多
关键词 AM/ampS共聚物压裂液 超高矿化度 流变性 交联流变动力学 大振幅振荡剪切
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高温条件下AMPS类缓凝剂缓凝作用效果分析
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作者 谢帅 张景富 +3 位作者 林荣壮 王建成 朱胡佳 谢雨辰 《当代化工》 CAS 2024年第1期1-6,共6页
缓凝剂的选配是高温水泥浆体系设计最关键的因素之一。为了合理地优选和适用缓凝剂,选用AMPS-IA共聚缓凝剂与大连G级油井水泥作用,通过高温高压稠化仪、X射线衍射仪、扫描电子显微镜和能谱仪等对水泥稠化性能、水化硬化产物的物质组成... 缓凝剂的选配是高温水泥浆体系设计最关键的因素之一。为了合理地优选和适用缓凝剂,选用AMPS-IA共聚缓凝剂与大连G级油井水泥作用,通过高温高压稠化仪、X射线衍射仪、扫描电子显微镜和能谱仪等对水泥稠化性能、水化硬化产物的物质组成和微观结构特征进行检测,对其适用性进行分析。结果表明:AMPS类缓凝剂作为高温缓凝剂具有良好的缓凝效果,稠化线形好且稠化时间长。但是重复实验评价缓凝剂稳定性过程中有一定概率出现缓凝剂失效,稠化曲线出现波动,造成水泥浆异常胶凝。产生胶凝的原因是缓凝剂聚合物分子对阳离子产生螯合-吸附作用,在物理搅拌作用下使得大量聚合物分子与水泥熟料成胶联状聚集在搅拌轴外部。 展开更多
关键词 ampS 缓凝剂 油井水泥 稠化时间 异常胶凝
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AMPK及mTOR与多囊卵巢综合征的关系及中药干预研究进展 被引量:1
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作者 马桦 齐大河 +2 位作者 陈雯玥 司雨 任青玲 《中华中医药学刊》 CAS 北大核心 2024年第2期164-170,共7页
多囊卵巢综合征(Polycystic ovary syndrome,PCOS)是一组生殖内分泌代谢紊乱的综合征,临床以稀发排卵、高雄激素体征、胰岛素抵抗为主要特征,其中育龄期发病率高,对女性生育力造成严重不良影响。PCOS的发生发展涉及多种信号通路,腺苷酸... 多囊卵巢综合征(Polycystic ovary syndrome,PCOS)是一组生殖内分泌代谢紊乱的综合征,临床以稀发排卵、高雄激素体征、胰岛素抵抗为主要特征,其中育龄期发病率高,对女性生育力造成严重不良影响。PCOS的发生发展涉及多种信号通路,腺苷酸活化蛋白激酶(AMP-activated protein kinase,AMPK)及哺乳动物雷帕霉素靶蛋白(Mammalian target of rapamycin,mTOR)作为细胞能量感受器是其中两个关键靶点。二者在PCOS各个发病部位包括下丘脑-垂体-卵巢轴、子宫内膜、脂肪与骨骼肌中发挥重要的调节作用,通过影响细胞自噬、氧化应激、炎症、线粒体功能、葡萄糖摄取等,促进卵泡的发育和成熟,改善胰岛素抵抗。近年来,中医药因其成分多样、靶点众多等优势广泛应用于临床,研究人员已对PCOS的发病以及中药治疗及改善PCOS的机制进行了大量研究,结果提示AMPK与mTOR相关通路在其中发挥关键作用。通过总结中药干预AMPK与mTOR及其相关通路治疗PCOS的研究结果,为临床治疗及基础研究提供参考。 展开更多
关键词 多囊卵巢综合征 腺苷酸活化蛋白激酶(ampK) 哺乳动物雷帕霉素靶蛋白(mTOR) 中药 作用机制
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Routine Supportive Supervision and Management of Medicines and Other Health Products and Technologies in Vihiga County, Kenya
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作者 Mukanya Collins Mudogo Oluhano Jerusa +6 位作者 Sikuku Eric Mwanje Inonda Justus Anyiendah Mary Amadi Mercy Riungu James Sumbi Victor Ottichilo Wilber 《Pharmacology & Pharmacy》 CAS 2023年第2期43-57,共15页
Health Products and Technologies (HPTs) are pivotal for an efficient health system. Availability and accessibility to affordable health products are critical indicators towards achieving universal health coverage. Rou... Health Products and Technologies (HPTs) are pivotal for an efficient health system. Availability and accessibility to affordable health products are critical indicators towards achieving universal health coverage. Routine supportive supervision, performance monitoring, recognition of efforts and client feedback are vital activities toward health supply chain system strengthening. This is a descriptive paper that describes a model of integrated commodity supportive supervision, and mentorship and its impact on various outcomes of health commodity management. Data were abstracted from the standardized scored checklists used during integrated commodity supportive supervision and supply chain audit in public health facilities in Vihiga County. Scores for the period 2020 to 2022 were analyzed on the eight key areas of interest. The analysis was done using Statistical Package for Social Sciences (SPSS version 26). Results are interpreted at 95% Confidence interval. This paper also shares findings from both quantitative and qualitative data from client exit and facility managers’ interviews. Six complete rounds of supervisions, three clients and service providers’ interviews, and three annual award events have been conducted. We observed trends across six data collections points and compared the results at first point or baseline (January-June 2020) to the results at the last point or end line (April-June 2022). Findings show significant improvements on the eight parameters in terms of mean scores as follows: resolution of issues from previous visits by 35.06% (46.75% - 81.81%);storage of HPTs by 17.41% (68.72% - 86.13%);inventory management by 28.16% (42.67% - 70.83%);availability and use of commodity data management information systems (MIS) tools by 22.39% (74.40% - 96.79%);verification of commodity data by 25.61% (65.56% - 91.17%);availability of guidelines and job aids for commodity management by 46.28% (36.65% - 82.93%). There was an improvement on the mean score on accountability by 20.22% (58.58% - 83.51%). The composite (final) score improved by 28.33% (56.19% - 84.52%). There was progressive narrowing of the standard deviations on all the indicators across the study period. This demonstrates that there is standardization of practices and positive competition among all the public health facilities. There were significant improvements on all the eight indicators. Routine integrated commodity supportive supervision has proven to be an effective high impact intervention in improving management of health products and technologies in Vihiga County, Kenya. 展开更多
关键词 MEDICINES Health Products and Technologies Health Commodities Supportive supervision Staff Motivation Customer Feedback
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Model-constrained and data-driven double-supervision acoustic impedance inversion
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作者 Dong-Feng Zhao Na-Xia Yang +2 位作者 Jin-Liang Xiong Guo-Fa Li Shu-Wen Guo 《Petroleum Science》 SCIE EI CSCD 2023年第5期2809-2821,共13页
Seismic impedance inversion is an important technique for structure identification and reservoir prediction.Model-based and data-driven impedance inversion are the commonly used inversion methods.In practice,the geoph... Seismic impedance inversion is an important technique for structure identification and reservoir prediction.Model-based and data-driven impedance inversion are the commonly used inversion methods.In practice,the geophysical inversion problem is essentially an ill-posedness problem,which means that there are many solutions corresponding to the same seismic data.Therefore,regularization schemes,which can provide stable and unique inversion results to some extent,have been introduced into the objective function as constrain terms.Among them,given a low-frequency initial impedance model is the most commonly used regularization method,which can provide a smooth and stable solution.However,this model-based inversion method relies heavily on the initial model and the inversion result is band limited to the effective frequency bandwidth of seismic data,which cannot effectively improve the seismic vertical resolution and is difficult to be applied to complex structural regions.Therefore,we propose a data-driven approach for high-resolution impedance inversion based on the bidirectional long short-term memory recurrent neural network,which regards seismic data as time-series rather than image-like patches.Compared with the model-based inversion method,the data-driven approach provides higher resolution inversion results,which demonstrates the effectiveness of the data-driven method for recovering the high-frequency components.However,judging from the inversion results for characterization the spatial distribution of thin-layer sands,the accuracy of high-frequency components is difficult to guarantee.Therefore,we add the model constraint to the objective function to overcome the shortages of relying only on the data-driven schemes.First,constructing the supervisor1 based on the bidirectional long short-term memory recurrent neural network,which provides the predicted impedance with higher resolution.Then,convolution constraint as supervisor2 is introduced into the objective function to guarantee the reliability and accuracy of the inversion results,which makes the synthetic seismic data obtained from the inversion result consistent with the input data.Finally,we test the proposed scheme based on the synthetic and field seismic data.Compared to model-based and purely data-driven impedance inversion methods,the proposed approach provides more accurate and reliable inversion results while with higher vertical resolution and better spatial continuity.The inversion results accurately characterize the spatial distribution relationship of thin sands.The model tests demonstrate that the model-constrained and data-driven impedance inversion scheme can effectively improve the thin-layer structure characterization based on the seismic data.Moreover,tests on the oil field data indicate the practicality and adaptability of the proposed method. 展开更多
关键词 Acoustic impedance inversion Model constraints Double supervision BiLSTM neural network Reservoir structure characterization
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Human-pose estimation based on weak supervision
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作者 Xiaoyan HU Xizhao BAO +1 位作者 Guoli WEI Zhaoyu LI 《Virtual Reality & Intelligent Hardware》 EI 2023年第4期366-377,共12页
Background In computer vision,simultaneously estimating human pose,shape,and clothing is a practical issue in real life,but remains a challenging task owing to the variety of clothing,complexity of de-formation,shorta... Background In computer vision,simultaneously estimating human pose,shape,and clothing is a practical issue in real life,but remains a challenging task owing to the variety of clothing,complexity of de-formation,shortage of large-scale datasets,and difficulty in estimating clothing style.Methods We propose a multistage weakly supervised method that makes full use of data with less labeled information for learning to estimate human body shape,pose,and clothing deformation.In the first stage,the SMPL human-body model parameters were regressed using the multi-view 2D key points of the human body.Using multi-view information as weakly supervised information can avoid the deep ambiguity problem of a single view,obtain a more accurate human posture,and access supervisory information easily.In the second stage,clothing is represented by a PCA-based model that uses two-dimensional key points of clothing as supervised information to regress the parameters.In the third stage,we predefine an embedding graph for each type of clothing to describe the deformation.Then,the mask information of the clothing is used to further adjust the deformation of the clothing.To facilitate training,we constructed a multi-view synthetic dataset that included BCNet and SURREAL.Results The Experiments show that the accuracy of our method reaches the same level as that of SOTA methods using strong supervision information while only using weakly supervised information.Because this study uses only weakly supervised information,which is much easier to obtain,it has the advantage of utilizing existing data as training data.Experiments on the DeepFashion2 dataset show that our method can make full use of the existing weak supervision information for fine-tuning on a dataset with little supervision information,compared with the strong supervision information that cannot be trained or adjusted owing to the lack of exact annotation information.Conclusions Our weak supervision method can accurately estimate human body size,pose,and several common types of clothing and overcome the issues of the current shortage of clothing data. 展开更多
关键词 Human pose estimation Clothing estimation Weak supervision
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Strategic interaction between institutional investors and supervision department:a theoretical analysis of low‑price collusion in SBIC
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作者 Xin Li Zhuming Chen 《Financial Innovation》 2023年第1期1313-1348,共36页
We introduce evolutionary game method to analyze low-price collusion in inquiry market of Sci-Tech Innovation Board of China(SIBC)from the perspective of strategic interaction between large institutional investors(LII... We introduce evolutionary game method to analyze low-price collusion in inquiry market of Sci-Tech Innovation Board of China(SIBC)from the perspective of strategic interaction between large institutional investors(LIIs),small and medium-sized institutional investors(SMIIs),and supervision department(SD).The results show that supervision behaviors of SD,and quotation behaviors of institutional investors,are subject to supervision conditions.Under the condition that benefits of tough supervision are lower a lot than minimum benefits of light supervision(light supervision condition),SD will choose light supervision and institutional investors will turn to illegal quotation in response.Finally,a steady-state equilibrium with low-price collusion will form in SIBC’s inquiry market even with a large supervision penalty for illegal quotation.On the contrary,under the condition that benefits of tough supervision are higher a lot than maximum benefits of light supervision(tough supervision condition)and with a large penalty for illegal quotation,SD and institutional investors will choose tough supervision and legal quotation.Further numerical simulations under light supervision condition show that:(1)High-price culling rule will become a booster for low-price collusion and accelerate SMIIs’evolutionary process to imitative quotation.(2)Blindly increasing penalties for illegal quotation or reducing the culling rate is not an appropriate approach to solve the problem of low-price collusion since it cannot shift supervision condition from light into tough and make SD supervise toughly.(3)Institutional investors’choices of quotation strategies are more volatile and highly susceptible to supervision behaviors of SD when facing exogenous uncertainty.Therefore,the keys to solving the problem of low-price collusion are shifting supervision condition from light into tough through increasing incremental benefits of tough supervision,and providing institutional investors with a stable and predictable supervision policy.In conclusion,the creation of a fair inquiry market doesn’t only depend on restraint and punishment to institutional investors,but also requires the establishment of supervision mechanism those are compatible with market-based inquiry. 展开更多
关键词 Sci-Tech Innovation Board of China Low-price collusion supervision condition Tripartite evolutionary game
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AMP活化蛋白激酶抑制肾纤维化的作用机制研究进展
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作者 孙宇石 李柏均 程帆 《山东医药》 CAS 2024年第14期87-90,共4页
肾脏主要由肾小管上皮细胞的脂肪酸β氧化提供能量,脂毒性物质的累积、糖酵解代谢产物的堆积以及能量缺陷等都是肾纤维化的诱导因素。在肾纤维化过程中,肾脏的产能方式发生显著改变,脂肪酸氧化减少,脂质积累增加,糖酵解成为肾脏的主要... 肾脏主要由肾小管上皮细胞的脂肪酸β氧化提供能量,脂毒性物质的累积、糖酵解代谢产物的堆积以及能量缺陷等都是肾纤维化的诱导因素。在肾纤维化过程中,肾脏的产能方式发生显著改变,脂肪酸氧化减少,脂质积累增加,糖酵解成为肾脏的主要代谢方式。AMP活化蛋白激酶(AMPK)作为能量代谢的中心调控分子,在细胞损伤过程中起到抑制糖原合成和糖异生、促进葡萄糖摄取、调配糖酵解以改善能量失衡、抑制脂质合成、促进脂肪分解和脂肪酸氧化等作用,从而抑制肾纤维化进展。阐明AMPK介导的能量代谢抑制肾纤维化进展的机制,有助于为肾纤维化的治疗提供新的思路。 展开更多
关键词 肾纤维化 amp活化蛋白激酶 脂质代谢 糖代谢
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