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From Control to Empowerment:A Paradigm Shift in the Discourse of Educational Supervision
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作者 Jinhua Zhou Kai Zhang 《Journal of Contemporary Educational Research》 2024年第9期176-180,共5页
As educational reforms intensify and societal emphasis shifts towards empowerment,the traditional discourse paradigm of management and control in educational supervision faces growing challenges.This paper explores th... As educational reforms intensify and societal emphasis shifts towards empowerment,the traditional discourse paradigm of management and control in educational supervision faces growing challenges.This paper explores the transformation of this discourse paradigm through the lens of empowerment,analyzing its distinct characteristics,potential pathways,and effective strategies.This paper begins by reviewing the concept of empowerment and examining the current research landscape surrounding the discourse paradigm in educational supervision.Subsequently,we conduct a comparative analysis of the“control”and“empowerment”paradigms,highlighting their essential differences.This analysis illuminates the key characteristics of an empowerment-oriented approach to educational supervision,particularly its emphasis on dialogue,collaboration,participation,and,crucially,empowerment itself.Ultimately,this research advocates for a shift in educational supervision towards an empowerment-oriented discourse system.This entails a multi-pronged approach:transforming ingrained beliefs,embracing renewed pedagogical concepts,fostering methodological innovation,and optimizing existing mechanisms and strategies within educational supervision.These changes are proposed to facilitate the more effective alignment of educational supervision with the pursuit of high-quality education. 展开更多
关键词 Educational supervision Paradigm shift CONTROL EMPOWERMENT
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Study on Independent Supervision Function of Design Assurance System
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作者 SUI Lijun MA Congyao WANG Jianyuan 《International Journal of Plant Engineering and Management》 2024年第3期185-192,共8页
The effective operation of a design assurance system cannot be achieved without the effective performance of the independent supervision function.As one of the core functions of the design assurance system,the purpose... The effective operation of a design assurance system cannot be achieved without the effective performance of the independent supervision function.As one of the core functions of the design assurance system,the purpose of the independent supervision function is to ensure that the system operates within the scope of procedures and manuals.At present,the function of independent supervision is a difficult and confusing issue for various original equipment manufacturers as well as suppliers,and there is an urgent requirement to put forward relevant requirements and form relevant methods.Based on the above mentioned objective,the basic requirements of the independent supervision function of design assurance system were studied,the problems and deficiencies in the organization,staffing,and methods existing in the current independent supervision function were analyzed,the improvement suggestions and measures for the performance of the independent supervision function from the aspects of the organization,staffing,procedures,and suppliers were put forward.The present work and conclusions provide guidance and direction for the effective operation of the design assurance system. 展开更多
关键词 design of assurance systems independent supervision functions system operation internal audits SUPPLIERS
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Analysis on Results of Test and Supervision for Oxidative Hair Dyes Samples in Yangzhou from 2013 to 2018
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作者 Ling Zhen Ding Susu +3 位作者 Liu Hui Yao Jingjing Zhu Qingli Wang Wei 《China Detergent & Cosmetics》 2019年第2期58-61,共4页
According to Hygienic Standard for Cosmetics (2007 Edition) and Safety and Technical Standards for Cosmetics (2015 Edition), Oxidative Hair Dyes were supervised and inspected in Yangzhou from 2013 to 2018. P-Phenylene... According to Hygienic Standard for Cosmetics (2007 Edition) and Safety and Technical Standards for Cosmetics (2015 Edition), Oxidative Hair Dyes were supervised and inspected in Yangzhou from 2013 to 2018. P-Phenylenediamine and other 6 kinds of components from 85 batches of samples were determined and analyzed by HPLC. Only one batch of samples had not passed test, o-Phenylenediamine was detected that should be prohibited in hair dyes. The results of supervision and inspection for oxidative hair dyes were good in Yangzhou from 2013 to 2018. 展开更多
关键词 OXIDATIVE HAIR DYES COSMETICS P-PHENYLENEDIAMINE supervision and inspection
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Supervision of Public Opinion under the Background of Social Co-governance-Take the Changsheng Vaccine Incident as an Example
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作者 Ma Xiaonan Tian Lijuan 《Asian Journal of Social Pharmacy》 2023年第3期198-205,共8页
Objective To provide reference for the news media to give play to the role of public opinion supervision in time based on the background of drug safety and social co-governance.Methods The method of case analysis was ... Objective To provide reference for the news media to give play to the role of public opinion supervision in time based on the background of drug safety and social co-governance.Methods The method of case analysis was used to make a retrospective study on the Changsheng vaccine incident in 2018.Then the role of mainstream media,pharmaceutical media,and self-media in the supervision of public opinion was investigated.Results and Conclusion Both mainstream and pharmaceutical media played an excellent role in supervising the Changchun Changsheng vaccine incident.However,the content published by some pharmaceutical media was hard to understand by ordinary people.Besides,the role of self-media in public opinion supervision was polarized.Some self-media closely kept pace with mainstream media in public opinion supervision.Other self-media unilaterally pursued the click rate,publishing false information to guide wrong public opinion.The news media should optimize the supervision efficiency of drug safety.On the one hand,pharmaceutical media should pay attention to the fact that readers may not understand the difficult terms because they are not professional.On the other hand,self-media practitioners should improve their professional quality so that they will not publish some fake news to mislead public opinion. 展开更多
关键词 social co-governance Changchun Changsheng vaccine incident news media public opinion supervision
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Distant Supervision方法中对齐数据的聚类去噪 被引量:1
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作者 朱兆龙 欧阳丹彤 叶育鑫 《吉林大学学报(理学版)》 CAS CSCD 北大核心 2014年第2期280-284,共5页
基于聚类和寻找表达语义关系的句子模式,提出一种基于聚类去噪的distant supervision方法,解决了传统distant supervision方法在知识库和文本集对齐阶段会引入噪声数据的问题,得到了一种改进的distant supervision方法.实验结果表明,该... 基于聚类和寻找表达语义关系的句子模式,提出一种基于聚类去噪的distant supervision方法,解决了传统distant supervision方法在知识库和文本集对齐阶段会引入噪声数据的问题,得到了一种改进的distant supervision方法.实验结果表明,该方法可有效提高关系抽取任务的准确率. 展开更多
关键词 关系抽取 聚类 DISTANT supervision方法 去噪
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Key Points in the Supervision of Road Landscaping Project——A Case Study of the South 3^(rd) Ring Road in Yanta District of Xi'an City,China 被引量:1
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作者 弓弼 魏代谋 《Journal of Landscape Research》 2011年第6期71-74,共4页
Characteristics of road landscaping were analyzed,as a kind of "linear" landscaping,it could be classified into urban street landscaping,urban ring road and expressway landscaping.Differences between road la... Characteristics of road landscaping were analyzed,as a kind of "linear" landscaping,it could be classified into urban street landscaping,urban ring road and expressway landscaping.Differences between road landscaping projects and other garden landscaping projects or construction projects were analyzed.Landscaping project for the South 3rd Ring Road of Xi'an City(in Yanta District)was taken for an example to summarize construction experience,discuss present conditions and features of garden landscaping supervision in China.The artistic and physiological properties of garden landscaping,as well as the supervision during maintenance period were stressed,but it was also pointed out that garden landscaping supervision in China was actually not given sufficient attention.On this basis,key points in the supervision of road landscaping project were proposed:understanding design ideas,selection of landscape plants,quality control of concealed works,prevention and control of diseases and insects,construction safety management and overall image;only by effectively implementing these key points,a high-quality road landscaping project could be realized to improve the image of a city and beautify its environment. 展开更多
关键词 ROAD LANDSCAPING project Construction supervision The South 3rd RING ROAD of Xi’an
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人参皂苷Rc通过调控AMPK通路介导的细胞焦亡减轻小鼠脑缺血再灌注损伤
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作者 李良勇 马莉 +1 位作者 李亚军 梅灯 《中国病理生理杂志》 CAS CSCD 北大核心 2024年第5期844-851,共8页
目的:探讨人参皂苷Rc对脑缺血再灌注损伤(CIRI)小鼠的保护作用,并从细胞焦亡角度阐释其作用机制。方法:将C57BL/6小鼠随机分为假手术(sham)组、大脑中动脉闭塞/再灌注(MCAO/R)组、人参皂苷Rc+MCAO/R组(Rc组)、AMP活化蛋白激酶(AMPK)激... 目的:探讨人参皂苷Rc对脑缺血再灌注损伤(CIRI)小鼠的保护作用,并从细胞焦亡角度阐释其作用机制。方法:将C57BL/6小鼠随机分为假手术(sham)组、大脑中动脉闭塞/再灌注(MCAO/R)组、人参皂苷Rc+MCAO/R组(Rc组)、AMP活化蛋白激酶(AMPK)激动剂阿卡地新(AICAR)+MCAO/R组(agonist组)和人参皂苷Rc+MCAO/R+AMPK抑制剂Compound C组(Rc+inhibitor组)。对agonist组小鼠给予AICAR(500 mg/kg)腹腔注射,Rc+inhibitor组小鼠给予Compound C(20 mg/kg)腹腔注射;Rc组和Rc+inhibitor组小鼠造模后给予人参皂苷Rc(40 mg/kg)灌胃,每天一次,连续7 d;sham组和MCAO/R组则给予等体积的纯化水。Zea Longa评分观察小鼠神经功能缺损程度,TTC染色观察小鼠脑梗死体积,干湿重法观察小鼠脑水肿程度,HE染色观察小鼠脑组织病理形态的改变,RT-qPCR和Western blot检测小鼠脑组织AMPK、核苷酸结合寡聚化结构域样受体蛋白3(NLRP3)、胱天蛋白酶1(caspase-1)和焦亡效应蛋白消皮素D(GSDMD)的表达,ELISA检测炎症因子白细胞介素1β(IL-1β)和IL-18的含量。结果:Rc组和agonist组小鼠神经功能缺损症状减轻,脑梗死体积减小,脑水肿和神经元病理损伤受到抑制,脑组织pAMPK/AMPK比值和AMPK mRNA表达水平升高,细胞焦亡相关蛋白NLRP3、caspase-1和GSDMD的mRNA和蛋白表达水平降低,炎症因子IL-1β和IL-18表达水平降低。Rc+inhibitor组小鼠脑组织p-AMPK/AMPK比值和AMPK mRNA表达水平较Rc组显著下降(P<0.05),NLRP3、caspase-1和GSDMD的mRNA和蛋白表达水平,以及IL-1β和IL-18表达水平显著升高(P<0.05),小鼠神经功能缺损程度评分升高,脑梗死体积增大,脑水肿和神经元病理损伤显著加重(P<0.05)。结论:人参皂苷Rc可能通过活化AMPK通路抑制NLRP3/caspase-1/GSDMD介导的细胞焦亡,从而减轻小鼠CIRI。 展开更多
关键词 人参皂苷Rc 脑缺血再灌注损伤 amp活化蛋白激酶 细胞焦亡
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黄芩苷调节cAMP/PKA/CREB信号通路对湿疹大鼠皮肤屏障功能的影响 被引量:3
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作者 王首帆 徐宜厚 +1 位作者 徐爱琴 朱立宏 《天津医药》 CAS 2024年第2期148-153,共6页
目的探讨黄芩苷(BA)调节环磷酸腺苷(cAMP)/蛋白激酶A(PKA)/cAMP反应元件结合蛋白(CREB)通路对湿疹大鼠皮肤屏障功能的影响。方法将SD大鼠随机分为对照组(NC组)、Model组、低剂量BA组(BA-L组,25 mg/kg)、中剂量BA组(BA-M组,50 mg/kg)、... 目的探讨黄芩苷(BA)调节环磷酸腺苷(cAMP)/蛋白激酶A(PKA)/cAMP反应元件结合蛋白(CREB)通路对湿疹大鼠皮肤屏障功能的影响。方法将SD大鼠随机分为对照组(NC组)、Model组、低剂量BA组(BA-L组,25 mg/kg)、中剂量BA组(BA-M组,50 mg/kg)、高剂量BA组(BA-H组,100 mg/kg)、泼尼松组(PNS组,25 mg/kg)、BAH+cAMP抑制剂(SQ22536)组(100 mg/kg+2.13 mg/kg)、BA-H+PKA抑制剂(H-89)组(100 mg/kg+5 mg/kg),每组12只。除NC组外,其余组大鼠均构建湿疹大鼠模型。建模成功2 d后,分组进行给药处理。检测湿疹面积及严重度指数(EASI)评分、经皮肤水分流失量(TEWL)、角质层含水量(WCSC)变化;酶联免疫吸附试验(ELISA)检测大鼠血清中免疫球蛋白E(IgE)、干扰素-γ(IFN-γ)、白细胞介素-4(IL-4)水平及大鼠背部受试区皮损组织中c AMP蛋白表达;HE染色检测大鼠背部受试区皮损组织病理变化;Western blot检测大鼠背部受试区皮损组织中水通道蛋白3(AQP3)、cathelicidin相关抗菌肽(CRAMP)、p-PKA、p-CREB蛋白表达。结果与NC组比较,Model组大鼠背部受试区皮损组织病理损伤严重,EASI评分、TEWL、IgE、IL-4水平升高,WCSC、IFN-γ水平、AQP3、CRAMP、cAMP、p-PKA、p-CREB蛋白水平降低(P<0.05)。与Model组比较,BA-L组、BA-M组、BA-H组、PNS组大鼠背部受试区皮损组织病理损伤减轻,EASI评分、TEWL、IgE、IL-4水平降低,WCSC、IFN-γ水平、AQP3、CRAMP、cAMP、p-PKA、p-CREB蛋白水平升高(P<0.05);且BA-L组、BA-M组、BA-H组上述指标变化呈剂量依赖性。SQ22536或H-89减弱了高剂量BA对湿疹大鼠皮肤屏障功能的改善作用。结论BA可能通过激活cAMP/PKA/CREB信号通路改善湿疹大鼠皮肤屏障功能。 展开更多
关键词 湿疹 黄芩苷 环磷酸腺苷/蛋白激酶A/camp反应元件结合蛋白通路 皮肤屏障功能
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基于NOD2介导的AMPK/mTOR信号通路探讨宫颈癌细胞恶性行为的机制
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作者 杜瑞亭 伍东月 +1 位作者 郭清民 靳冬梅 《安徽医科大学学报》 CAS 北大核心 2024年第2期316-324,共9页
目的 基于核苷酸结合寡聚化结构域受体2(NOD2)介导的AMP活化蛋白激酶(AMPK)/雷帕霉素靶蛋白(mTOR)信号通路探讨宫颈癌(CC)细胞恶性行为的机制。方法 生物信息学分析确定NOD2在CC组织中的表达。将靶向NOD2(shNOD2)、shRNAs阴性对照(shNC... 目的 基于核苷酸结合寡聚化结构域受体2(NOD2)介导的AMP活化蛋白激酶(AMPK)/雷帕霉素靶蛋白(mTOR)信号通路探讨宫颈癌(CC)细胞恶性行为的机制。方法 生物信息学分析确定NOD2在CC组织中的表达。将靶向NOD2(shNOD2)、shRNAs阴性对照(shNC)以及NOD2过表达(NOD2)质粒和载体(Vec)转染CC细胞。通过CCK-8测定、集落形成和Transwell细胞侵袭测定来确定NOD2对CC细胞生长的影响。通过高通量RNA测序(RNA-Seq)进行转录组分析。Western blot试验检测细胞系中NOD2、AMPK/mTOR信号通路和自噬蛋白的表达。24只雌性BALB/c裸鼠随机分为4组,每组6只:载体组(Vec组)、NOD2过表达组(NOD2组)、shNC组和shNOD2组。构建小鼠远处转移模型,监测肺转移的荧光强度,计数肺转移结节的数量。结果 在线数据库分析显示,NOD2在CC组织中表达明显高于正常组织,并且不同分期的CC中NOD2的mRNA表达差异有统计学意义(P<0.05)。此外,NOD2的高表达与较差的总生存期和无病生存期相关(P<0.05)。NOD2过表达对CC细胞增殖、集落形成、迁移和侵袭具有促进作用,而NOD2敲低则相反。与体外结果一致,在转移的小鼠尾静脉注射模型中,NOD2组CC细胞的肺定殖、肺转移灶较Vec组增加(P<0.05),而shNOD2组CC细胞的肺定殖、肺转移灶较shNC组减少(P<0.05)。RNA-Seq结果显示NOD2表达与AMPK信号激活、mTOR信号抑制、自噬调节途径激活和自噬体形成显著相关。与shNC组相比,shNOD2组磷酸化AMPK、LC3蛋白表达水平减少(P<0.05),磷酸化mTOR、p62蛋白表达水平增加(P<0.05);与Vec组相比,NOD2组LC3、AMPK蛋白表达水平增加(P<0.05),磷酸化mTOR、p62蛋白表达水平减少(P<0.05)。与shNC组相比,shNOD2组GFP-mRFP-LC3的点积累减少(P<0.05);与Vec组相比,GFP-mRFP-LC3的点积累增加(P<0.05)。结论 NOD2可能通过AMPK/mTOR信号促进CC增殖、迁移和侵袭,其作用机制部分涉及自噬激活。 展开更多
关键词 核苷酸结合寡聚化结构域受体2 amp活化蛋白激酶 雷帕霉素靶蛋白 宫颈癌细胞 自噬
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川陈皮素调节AMPK/NLRP3信号通路对脂多糖诱导的肾小球系膜细胞炎性损伤的影响
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作者 罗丹 王燕 +1 位作者 丁旭 胡耀 《中药新药与临床药理》 CAS CSCD 北大核心 2024年第2期224-229,共6页
目的探讨川陈皮素(Nobiletin)调节AMP激活的蛋白激酶(AMPK)/NOD样受体蛋白3(NLRP3)信号通路对脂多糖(LPS)诱导的肾小球系膜细胞HBZY-1炎性损伤的影响。方法将HBZY-1细胞分为5组:正常组、LPS组(100 ng·m L^(-1)LPS)、川陈皮素组(100... 目的探讨川陈皮素(Nobiletin)调节AMP激活的蛋白激酶(AMPK)/NOD样受体蛋白3(NLRP3)信号通路对脂多糖(LPS)诱导的肾小球系膜细胞HBZY-1炎性损伤的影响。方法将HBZY-1细胞分为5组:正常组、LPS组(100 ng·m L^(-1)LPS)、川陈皮素组(100 ng·m L^(-1)LPS+40μmol·L^(-1)川陈皮素)、AMPK/NLRP3信号通路抑制剂雷帕霉素组(100 ng·m L^(-1)LPS+0.5μmol·L^(-1)雷帕霉素)、川陈皮素+雷帕霉素组(100 ng·m L^(-1)LPS+40μmol·L^(-1)川陈皮素+0.5μmol·L^(-1)雷帕霉素)。MTT法检测HBZY-1细胞毒性和增殖;ELISA法检测HBZY-1细胞白细胞介素(IL)-1β、IL-6、肿瘤坏死因子α(TNF-α)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、谷胱甘肽(GSH)含量;流式细胞术检测细胞凋亡;Western Blot法检测AMPK/NLRP3信号通路蛋白水平。结果与正常组比较,LPS组CAT、SOD、GSH水平、细胞OD值以及AMPK蛋白水平明显降低(P<0.05),细胞凋亡率、IL-1β、IL-6、TNF-α含量以及NLRP3蛋白水平明显升高(P<0.05)。与LPS组比较,川陈皮素组CAT、SOD、GSH水平、OD值以及AMPK蛋白水平明显升高(P<0.05),细胞凋亡率、IL-1β、IL-6、TNF-α含量以及NLRP3蛋白水平明显下降(P<0.05);而雷帕霉素组以上指标均呈现与川陈皮素组相反的趋势(P<0.05)。与川陈皮素组比较,川陈皮素+雷帕霉素组以上指标均呈现与川陈皮素组相反的趋势(P<0.05)。结论川陈皮素可能通过上调AMPK/NLRP3信号通路减轻LPS诱导的肾小球系膜细胞细胞炎性损伤。下调AMPK/NLRP3信号通路可消除川陈皮素对LPS诱导的肾小球系膜细胞细胞炎性损伤的改善作用。 展开更多
关键词 川陈皮素 amp激活的蛋白激酶/NOD样受体蛋白3(ampK/NLRP3)信号通路 脂多糖 肾小球系膜细胞 HBZY-1 炎性损伤
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基于AMPK/sirt1/RANKL/OPG通路研究补阳还五汤促进骨质疏松性骨折大鼠愈合的机制 被引量:1
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作者 林玉玲 李杰玉 +3 位作者 李增一 刘琳 籍胤玺 金毅 《中国老年学杂志》 CAS 北大核心 2024年第1期133-137,共5页
目的基于AMP活化蛋白激酶(AMPK)/沉默信息调节因子(sirt)1/核转录因子(NF)-κB受体活化蛋白配体(RANKL)/骨保护素(OPG)信号通路,探究补阳还五汤促进骨质疏松性骨折大鼠骨折愈合的机制。方法将大鼠随机分成假手术组、模型组、补阳还五汤... 目的基于AMP活化蛋白激酶(AMPK)/沉默信息调节因子(sirt)1/核转录因子(NF)-κB受体活化蛋白配体(RANKL)/骨保护素(OPG)信号通路,探究补阳还五汤促进骨质疏松性骨折大鼠骨折愈合的机制。方法将大鼠随机分成假手术组、模型组、补阳还五汤低剂量组(6.25 g/kg)、补阳还五汤中剂量组(12.50 g/kg)和补阳还五汤高剂量组(25.00 g/kg)。除假手术组外,其余组均构建骨质疏松性骨折模型,并给予相应剂量药物干预。检测大鼠骨密度(BMD)值、骨折愈合评分及骨痂体积;自动生化分析仪测定血清碱性磷酸酶(ALP)、骨钙素、钙和磷水平;酶联免疫吸附试验(ELISA)检测股骨组织中的丙二醛(MDA)、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)水平;苏木素-伊红(HE)染色观察各组大鼠骨痂组织的病理形态;Western印迹检测股骨组织中AMPK/sirt1/RANKL/OPG通路蛋白表达。结果与假手术组相比,模型组骨痂骨小梁分布稀疏,成骨细胞大量减少且有纤维组织生成,骨痂体积、血清中ALP、骨钙素、钙和磷水平及股骨组织中MDA水平、RANKL蛋白水平显著升高(P<0.05),BMD值、骨折愈合评分、骨组织中SOD和GSH-Px水平和AMPK、sirt1、OPG蛋白水平显著降低(P<0.05)。与模型组相比,补阳还五汤低、中、高剂量组骨痂骨小梁分布较为密集,成骨细胞增加,骨痂体积、血清中ALP、骨钙素、钙和磷水平及股骨组织中MDA水平、RANKL蛋白水平显著降低(P<0.05),BMD值、骨折愈合评分、骨组织中SOD和GSH-Px水平和AMPK、sirt1、OPG蛋白水平显著升高(P<0.05)。结论补阳还五汤可能通过抑制氧化应激反应,调节AMPK/sirt1/RANKL/OPG通路,发挥对骨质疏松骨折大鼠的治疗作用。 展开更多
关键词 补阳还五汤 骨质疏松性骨折 氧化应激 amp活化蛋白激酶(ampK)/沉默信息调节因子(sirt)1/核转录因子(NF)-κB受体活化蛋白配体(RANKL)/骨保护素(OPG)通路
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基于AMPK通路探讨茶多酚改善糖尿病大鼠糖脂代谢机制
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作者 黄苏萍 林欢 +2 位作者 曾淑华 杨柳媛 许陆达 《山东中医药大学学报》 2024年第2期192-200,共9页
目的:探讨茶多酚从AMP活化蛋白激酶(AMPK)通路途径调控糖尿病大鼠糖脂代谢的机制。方法:经糖耐量试验筛选6周龄SD大鼠45只,按随机数字表法分为正常组10只和造模组35只,造模组糖尿病造模成功后剩余30只,根据体质量分层法和血糖水平分为... 目的:探讨茶多酚从AMP活化蛋白激酶(AMPK)通路途径调控糖尿病大鼠糖脂代谢的机制。方法:经糖耐量试验筛选6周龄SD大鼠45只,按随机数字表法分为正常组10只和造模组35只,造模组糖尿病造模成功后剩余30只,根据体质量分层法和血糖水平分为模型组、二甲双胍组和茶多酚组,每组各10只。各组均于每日下午灌胃,正常组与模型组给予无菌生理盐水,二甲双胍组给予二甲双胍水溶液,茶多酚组给予茶多酚。干预120 d后,检测各组大鼠的空腹血糖(FBG)、总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)和低密度脂蛋白胆固醇(LDL-C);酶联免疫吸附试验法检测肝脏乙酰辅酶A羧化酶(ACC)和激素敏感性脂肪酶(HSL);实时荧光定量法检测肝脏ACCmRNA、转录因子胆固醇调节元件结合蛋白1(SREBP-1)mRNA、3-羟基-3-甲基戊二酸单酰辅酶A还原酶(HMGCR)mRNA;免疫印迹法检测肝脏AMPKα1、磷酸化AMP活化蛋白激酶α1(P-AMPKα1)、SREBP1、HMGCR、肉毒碱棕榈酰转移酶1(CPT1)、葡萄糖转运蛋白4(GLUT4)和磷酸烯醇丙酮酸羧化激酶(PEPCK)。结果:与正常组比较,模型组大鼠的一般情况较差,FBG、TG、TC、LDL-C、ACC升高(P<0.05),SREBP1、HMGCR蛋白及mRNA表达升高(P<0.05),CPTI、PEPCK蛋白表达升高(P<0.05),HDL-C、HSL降低(P<0.05),AMPKα1、P-AMPKα1、GLUT4蛋白表达降低(P<0.05)。与模型组比较,茶多酚组大鼠的一般情况改善,TG、LDL-C、ACC降低(P<0.05),SREBP1、HMGCR蛋白及mRNA表达降低(P<0.05),PEPCK蛋白表达降低(P<0.05),HDL-C、HSL升高(P<0.05),AMPKα1、P-AMPKα1、GLUT4、CPT1蛋白表达升高(P<0.05)。与二甲双胍组比较,茶多酚组TC升高(P<0.05),FBG、TG、LDL-C、HDL-C、HSL、ACC、ACC mRNA、HMGCR mRNA、SREBP1 mRNA、AMPKα1、P-AMPKα1、SREBP1、HMGCR、CPT1、GLUT4、PEPCK相对表达量差异无统计学意义(P>0.05)。结论:茶多酚可能通过调节AMPK通路,影响HSL、HMGCR、SREBP1、CPTI、GLUT4和PEPCK,从而改善糖尿病大鼠糖脂代谢。 展开更多
关键词 糖尿病 茶多酚 二甲双胍 amp活化蛋白激酶通路 糖脂代谢 大鼠
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阿江榄仁酸由AMPK/mTOR/HO-1信号通路调控自噬对糖尿病视网膜病变影响
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作者 蒋晨 万新娟 +2 位作者 王绍飞 王晓虹 丁琳 《河北医药》 CAS 2024年第2期171-175,共5页
目的探讨阿江榄仁酸(arjunolic acid,AA)对糖尿病视网膜病变(diabetic retinopathy,DR)大鼠视网膜细胞自噬及AMPK/mTOR/HO-1信号通路的影响。方法以健康SD大鼠为研究对象,构建链脲佐菌素(STZ)诱导的糖尿病大鼠模型,随机分为对照组(Con)... 目的探讨阿江榄仁酸(arjunolic acid,AA)对糖尿病视网膜病变(diabetic retinopathy,DR)大鼠视网膜细胞自噬及AMPK/mTOR/HO-1信号通路的影响。方法以健康SD大鼠为研究对象,构建链脲佐菌素(STZ)诱导的糖尿病大鼠模型,随机分为对照组(Con)组、模型(STZ)组、AA低剂量(AAL,10 mg/kg)组和AA高剂量(AAH,10 mg/kg)组。连续给药10周后,HE染色检测视网膜组织病理结构;qRT-PCR检测视网膜组织白介素(IL)-1β、IL-6和线粒体丙酮酸转运载体(MPC)-1的mRNA表达;二氢乙锭(DHE)染色评估视网膜组织ROS产生;Western blot检测自噬和AMPK/mTOR/HO-1信号通路相关蛋白表达。结果与Con组比较,STZ组大鼠视网膜出现肿胀和空泡样变化等病理变化,中央视网膜ONL层厚度和细胞核计数明显降低(P<0.01);IL-1β、IL-6和MCP-1的mRNA水平显著增高(P<0.05);视网膜外核层(ONL)、内核层(INL)和神经节细胞层(GCL)中ROS产生增加(P<0.01);LC3II/I比率、HO-1和p-AMPK/AMPK蛋白表达显著降低,p62和p-mTOR/mTOR表达升高(P<0.01)。与STZ组比较,AAL和AAH组大鼠视网膜ONL厚度和细胞核计数逐渐升高,结构相对规整(P<0.05);AAH组IL-1β、IL-6和MCP-1的mRNA表达明显降低(P<0.05);视网膜ONL、INL和GCL中ROS产生逐渐降低(P<0.01);LC3II/I比率、p-AMPK/AMPK和HO-1表达逐渐升高,p62和p-mTOR/mTOR表达逐渐降低(P<0.01)。结论阿江榄仁酸可能是治疗DR的候选药物,可能机制为通过AMPK/mTOR/HO-1调节的自噬途径保护视网膜细胞免受STZ诱导的氧化应激和炎症损伤。 展开更多
关键词 阿江榄仁酸 糖尿病视网膜病变 ampK/mTOR/HO-1通路 自噬
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Problems of Rural Food Safety and Strategies of Constructing Supervision System 被引量:7
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作者 XIAO Yue-qiang 《Asian Agricultural Research》 2011年第7期54-57,79,共5页
This paper expounds the practical necessity of constructing diversified rural food safety supervision system as follows: it is the necessary requirements of guaranteeing people's health and life safety; it is an i... This paper expounds the practical necessity of constructing diversified rural food safety supervision system as follows: it is the necessary requirements of guaranteeing people's health and life safety; it is an important component of governmental function of social management and the logical extension of administrative responsibilities; it is the basis of maintaining order of rural society and constructing harmonious society. The main problems existing in the supervision of rural food safety are analyzed as follows: first, the legislative work of rural food safety lags behind to some extent; second, the supervision of governmental departments on rural food safety is insufficient; third, the industrial supervision mechanism of rural food security is not perfect; fourth, the role of rural social organizations in supervising food safety is limited; fifth, the farmers' awareness of food safety supervision is not strong. Based on these problems, the targeted strategies of constructing diversified rural food safety supervision system are put forward as follows: accelerate the legislation of rural food safety, and ensure that there are laws to go by; give play to the dominant role of government, and strengthen administrative supervision on rural food safety; perfect industrial convention of rural food safety, and improve industrial supervision mechanism; actively support the fostering of social organizations, and give play to the role of supervision of organizations; cultivate correct concept of rights and obligations of farmers, and form awareness of food safety supervision. 展开更多
关键词 Rural food safety supervision Main problems System construction China
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Research on effectiveness of coal mine safety supervision system reform on three types of collieries in China 被引量:7
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作者 Quanlong Liu Xinchun Li Fuyuan Guan 《International Journal of Coal Science & Technology》 EI CAS 2014年第3期376-382,共7页
Coal mine safety supervision system plays an important role in the coal mine safety management in China.However,the current supervision system is established on the basis of learning the advanced experience from other... Coal mine safety supervision system plays an important role in the coal mine safety management in China.However,the current supervision system is established on the basis of learning the advanced experience from other developed countries.It needs to be further improved according to national conditions.Therefore,the effectiveness of coal mine safety supervision system reform on three types of collieries are assessed by using time series analysis method based on comparative analysis of the supervision system before and after the reform in this paper.The regression results show that the structural reform is not conductive to the improvement of coal mine safety situation in the short term,but conductive significantly in the long term.Specifically,the effects in township coal mines are more significant than stateowned key coal mines in the long run,but negative effects also exist in the short term.The negative effects in state-owned key coal mines are non-significant compared with township coal mines.Moreover,the regression results are analyzed from the aspects of the closure policy of illegal small township coal mines at the end of 1998 and shortage of the new supervision system.Finally,the suggestions on improving the new supervision system are put forward based on the above analysis. 展开更多
关键词 Coal mine safety supervision Time series analysis Sinuctural reform Death rate per million tonnes Effectiveness of supervision
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基于主题模型的中文Distant Supervision噪声标注识别方法
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作者 刘绍毓 李弼程 +2 位作者 周杰 席耀一 唐浩浩 《信息工程大学学报》 2016年第3期303-308,共6页
针对Distant Supervision关系抽取方法训练语料存在大量噪声的问题,提出一种基于主题模型的噪声标注识别方法。该方法首先分析了中文Distant Supervision实体关系抽取方法面临的关系句子实例结构复杂的问题,然后利用自定义的模式以及模... 针对Distant Supervision关系抽取方法训练语料存在大量噪声的问题,提出一种基于主题模型的噪声标注识别方法。该方法首先分析了中文Distant Supervision实体关系抽取方法面临的关系句子实例结构复杂的问题,然后利用自定义的模式以及模式聚类实现模式表示与聚合,最后使用主题模型识别噪声标注。实验结果表明,文章方法能有效识别噪声标注,用滤除噪声标注后的数据训练实体关系抽取模型,实验证明经过噪声滤除后实体关系抽取性能得到显著改善。 展开更多
关键词 DISTANT supervision 关系抽取 噪声标注识别 主题模型 关系模式
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Online Supervision of Penicillin Cultivations Based on Rolling MPCA 被引量:9
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作者 汪志锋 袁景淇 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2007年第1期92-96,共5页
To reduce the variations of the production process in penicillin cultivations, a rolling multivariate statis-tical approach based on multiway principle component analysis (MPCA) is developed and used for fault diagnos... To reduce the variations of the production process in penicillin cultivations, a rolling multivariate statis-tical approach based on multiway principle component analysis (MPCA) is developed and used for fault diagnosis of penicillin cultivations. Using the moving data windows technique, the static MPCA is extended for use in dy-namic process performance monitoring. The control chart is set up using the historical data collected from the past successful batches, thereby resulting in simplification of monitoring charts, easy tracking of the progress in each batch run, and monitoring the occurrence of the observable upsets. Data from the commercial-scale penicillin fer-mentation process are used to develop the rolling model. Using this method, faults are detected in real time and the corresponding measurements of these faults are directly made through inspection of a few simple plots (t-chart, SPE-chart, and T2-chart). Thus, the present methodology allows the process operator to actively monitor the data from several cultivations simultaneously. 展开更多
关键词 multiway principle component analysis FERMENTATION online supervision fault diagnosis ROLLING
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Research on the Status Quo and Supervision Mechanism of Food Safety in China 被引量:4
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作者 Shengzhong DONG Fangxu XU +2 位作者 Siyuan TAO Longkun WU Xingang ZHAO 《Asian Agricultural Research》 2018年第2期32-33,38,共3页
Food safety supervision mechanism is a strong guarantee to promote the smooth implementation of China's food safety laws and regulations,and it is implemented through legal,administrative,economic,moral and other ... Food safety supervision mechanism is a strong guarantee to promote the smooth implementation of China's food safety laws and regulations,and it is implemented through legal,administrative,economic,moral and other integrated policy instruments,as well as media publicity,quality traceability,network tracking,information disclosure and other non-administrative means. Along with strengthening supervision and control means,the people's food safety in China is safeguarded,and the healthy development of the food industry is promoted. 展开更多
关键词 Food safety supervision mechanism Regulatory system
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Optimization of Safety Production Supervision Mode of Coalmining Enterprises 被引量:3
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作者 ZHOU Min XIAO Zhong-hai 《Journal of China University of Mining and Technology》 EI 2005年第4期348-353,共6页
In view of the fact that safety production supervision of coal mines in China features low efficacy, this paper applies principles of cybernetics to simulate the dynamic process of safety supervision, and proposes tha... In view of the fact that safety production supervision of coal mines in China features low efficacy, this paper applies principles of cybernetics to simulate the dynamic process of safety supervision, and proposes that institutional variables be controlled to support intermediate goals, which in turn contribute to the ultimate safety production objective. Rather than focusing all attention on safety issues of working faces, supervising departments of coalmines are advised to pay much more attention to institutional factors that may impact people’s attitude and behavior, which are responsible for most coalmine accidents. It is believed that such a shift of attention can effectively reduce coalmining production accidents and greatly enhance supervision efficacy. 展开更多
关键词 supervision mode control model supervision efficacy conducting mechanism
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To supervise or to self‑supervise:a machine learning based comparison on credit supervision 被引量:3
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作者 JoséAmérico Pereira Antunes 《Financial Innovation》 2021年第1期562-582,共21页
This study investigates the need for credit supervision as conducted by on-site banking supervisors.It builds on a real bank on-site credit examination to compare the performance of a hypothetical self-supervision app... This study investigates the need for credit supervision as conducted by on-site banking supervisors.It builds on a real bank on-site credit examination to compare the performance of a hypothetical self-supervision approach,in which banks themselves assess their loan portfolios without external intervention,with the on-site banking supervision approach of the Central Bank of Brazil.The experiment develops two machine learning classification models:the first model is based on good and bad ratings informed by banks,and the second model is based on past on-site credit portfolio examinations conducted by banking supervision.The findings show that the overall performance of the on-site supervision approach is consistently higher than the performance of the self-supervision approach,justifying the need for on-site credit portfolio examination as conducted by the Central Bank. 展开更多
关键词 Bank supervision Machine learning Loan loss provisions On-site credit supervision
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