Zusammenfassung: Die morphologischen Veranderungen des mechanisch bzw. Elektrisch gereizten Skelettmuskels von 20 Leichen mit erhaltener supravitaler Reagibilitat in fruh postmortalen Zeitintervalen zwischen 1 h 25 m...Zusammenfassung: Die morphologischen Veranderungen des mechanisch bzw. Elektrisch gereizten Skelettmuskels von 20 Leichen mit erhaltener supravitaler Reagibilitat in fruh postmortalen Zeitintervalen zwischen 1 h 25 min und 8 h 30 min wurden histologisch beobachtet. Zum Vergleichen wurden Skelettmuskeln von 17 Leichen ohne erhaltene supravitale Reagibilitat in spat postmortalen Zeitintervalen zwischen 10 60 hpm gleichfalls gereizt. Nach supravitaler mechanischer bzw. elektrischer Reizung zeigten sich Storungen der Faserintegritat mit Veranderungen der sarkoplasmatischen Grundsubstanz und bruchnahen Invaginationen des Sarkoplasmaschlauches sowie Kontraktionsbander und bandformige Sarkoplasmaverdichtungen. Derartige Befunde lieen sich weder in den intraindividuellen Negativkontrolen noch nach spatpostmortaler Reizung nachweisen. Der Mechnismus der supravitalen morphologischen Reaktion wurde diskutiert und weitere Forschung ist zu erwarten. Schluelworter展开更多
Aim: To investigate the effect of cryopreservation on the plasma membrane integrity in the head and tail regions ofindividual sperm, and the relationship between intact cryopreserved sperm and its motility and zona-fr...Aim: To investigate the effect of cryopreservation on the plasma membrane integrity in the head and tail regions ofindividual sperm, and the relationship between intact cryopreserved sperm and its motility and zona-free hamster oocytepenetration rate. Methods: The eosin Y exclusion and the hypoosmotic swelling tests were combined to form a sin-gle test (HOS-EY test) to identify the spermatozoa with four types of membrane integrity. Results: After cryop-reservation, there was a marked decline in the percentage of spermatozoa with Type Ⅳ membrane integrity (head mem-brane intact/tail membrane intact), and a significant increase in those with Type Ⅰ (head membrane damaged/tail mem-brane damaged) and Type Ⅲ (head membrane damaged/tail membrane intact) membrane integrity (n = 50, P <0.01). The value of Type Ⅲ integrity had a wide range of variability, whereas Type Ⅱ (head membrane intact/tailmembrane damaged) was uncommon after thawing. A high correlation was observed between the percentage of Type Ⅳintegrity and sperm motility ( n = 50, r = 0.74, P < 0.01 ). However, the values of Type Ⅳ integrity were usuallylower than those of post-thaw motility in most cryopreserved samples. The value of Type Ⅳ integrity did not correlatewith the sperm penetration rate ( n = 25, r = 0.22, P > 0.05). Conclusion: (1) The HOS-EY test has the advan-tage of showing four patterns of membrane integrity in individual spermatozoon; (2) Cryopreservation causes a signifi-cant membrane rupture in the head and tail regions of spermatozoa; Type Ⅲ is the main transitional state of membranecryodamage; (3) Cryodamage to head and tail membrane may occur independently; the presence of an intact tail mem-brane does not necessarily indicate the intacmess of head membrane. (4) Intact membranes are closely related to post-thaw motility, but do not reflect the fertilizing potential.展开更多
文摘Zusammenfassung: Die morphologischen Veranderungen des mechanisch bzw. Elektrisch gereizten Skelettmuskels von 20 Leichen mit erhaltener supravitaler Reagibilitat in fruh postmortalen Zeitintervalen zwischen 1 h 25 min und 8 h 30 min wurden histologisch beobachtet. Zum Vergleichen wurden Skelettmuskeln von 17 Leichen ohne erhaltene supravitale Reagibilitat in spat postmortalen Zeitintervalen zwischen 10 60 hpm gleichfalls gereizt. Nach supravitaler mechanischer bzw. elektrischer Reizung zeigten sich Storungen der Faserintegritat mit Veranderungen der sarkoplasmatischen Grundsubstanz und bruchnahen Invaginationen des Sarkoplasmaschlauches sowie Kontraktionsbander und bandformige Sarkoplasmaverdichtungen. Derartige Befunde lieen sich weder in den intraindividuellen Negativkontrolen noch nach spatpostmortaler Reizung nachweisen. Der Mechnismus der supravitalen morphologischen Reaktion wurde diskutiert und weitere Forschung ist zu erwarten. Schluelworter
文摘Aim: To investigate the effect of cryopreservation on the plasma membrane integrity in the head and tail regions ofindividual sperm, and the relationship between intact cryopreserved sperm and its motility and zona-free hamster oocytepenetration rate. Methods: The eosin Y exclusion and the hypoosmotic swelling tests were combined to form a sin-gle test (HOS-EY test) to identify the spermatozoa with four types of membrane integrity. Results: After cryop-reservation, there was a marked decline in the percentage of spermatozoa with Type Ⅳ membrane integrity (head mem-brane intact/tail membrane intact), and a significant increase in those with Type Ⅰ (head membrane damaged/tail mem-brane damaged) and Type Ⅲ (head membrane damaged/tail membrane intact) membrane integrity (n = 50, P <0.01). The value of Type Ⅲ integrity had a wide range of variability, whereas Type Ⅱ (head membrane intact/tailmembrane damaged) was uncommon after thawing. A high correlation was observed between the percentage of Type Ⅳintegrity and sperm motility ( n = 50, r = 0.74, P < 0.01 ). However, the values of Type Ⅳ integrity were usuallylower than those of post-thaw motility in most cryopreserved samples. The value of Type Ⅳ integrity did not correlatewith the sperm penetration rate ( n = 25, r = 0.22, P > 0.05). Conclusion: (1) The HOS-EY test has the advan-tage of showing four patterns of membrane integrity in individual spermatozoon; (2) Cryopreservation causes a signifi-cant membrane rupture in the head and tail regions of spermatozoa; Type Ⅲ is the main transitional state of membranecryodamage; (3) Cryodamage to head and tail membrane may occur independently; the presence of an intact tail mem-brane does not necessarily indicate the intacmess of head membrane. (4) Intact membranes are closely related to post-thaw motility, but do not reflect the fertilizing potential.
