We apply the localized surface plasrnon resonance （LSPR） of gold nanoparticles （GNPs） covalently coupled with cytochrorne c （cyt c） to create a nanobiosensor for detecting hydrogen sulfide （H2S） in the range o...We apply the localized surface plasrnon resonance （LSPR） of gold nanoparticles （GNPs） covalently coupled with cytochrorne c （cyt c） to create a nanobiosensor for detecting hydrogen sulfide （H2S） in the range of 15 lOOppb. Monolayer formation of GNPs on glass surface functionalized with 3-aminopropyltrirnethoxysilane （APTMS） is performed for fabricating a chip-based format of the optical transducer. By chemical introduction of short-chain thiol derivatives on cyt c protein shell via its lysine residues, a very fast self-assembled rnonolayer （SAM） of cyt c is formed on the GNPs. Significant shifts in the LSPR peak （△λLSPR） are observed by reacting H2S with cyt c. Results show a linear relationship between △λLSPR and H2S concentration. Furthermore, shifts in the LSPR peak are reversible and the peak positions return to their pre-exposure values once the H2S is removed. The experirnental results strongly indicate that the protein based LSPR chip can be successfully used as a simple, fast, sensitive and quantitative sensor for H2S detection.展开更多
A metallic nanostructured array that scatters radiation toward a thin metallic layer generates surface plasmon resonances for normally incident light. The location of the minimum of the spectral reflectivity serves to...A metallic nanostructured array that scatters radiation toward a thin metallic layer generates surface plasmon resonances for normally incident light. The location of the minimum of the spectral reflectivity serves to detect changes in the index of refraction of the medium under analysis. The normal incidence operation eases its integration with optical fibers. The geometry of the arrangement and the material selection are changed to optimize some performance parameters as sensitivity, figure of merit, field enhancement, and spectral width. This optimization takes into account the feasibility of the fabrication. The evaluated results of sensitivity(1020 nm/RIU)and figure of merit(614 RIU^(-1)) are competitive with those previously reported.展开更多
文摘We apply the localized surface plasrnon resonance （LSPR） of gold nanoparticles （GNPs） covalently coupled with cytochrorne c （cyt c） to create a nanobiosensor for detecting hydrogen sulfide （H2S） in the range of 15 lOOppb. Monolayer formation of GNPs on glass surface functionalized with 3-aminopropyltrirnethoxysilane （APTMS） is performed for fabricating a chip-based format of the optical transducer. By chemical introduction of short-chain thiol derivatives on cyt c protein shell via its lysine residues, a very fast self-assembled rnonolayer （SAM） of cyt c is formed on the GNPs. Significant shifts in the LSPR peak （△λLSPR） are observed by reacting H2S with cyt c. Results show a linear relationship between △λLSPR and H2S concentration. Furthermore, shifts in the LSPR peak are reversible and the peak positions return to their pre-exposure values once the H2S is removed. The experirnental results strongly indicate that the protein based LSPR chip can be successfully used as a simple, fast, sensitive and quantitative sensor for H2S detection.
基金Funding.Ministerio de Economía y Competitividad(MINECO)(TEC2013-40442)Ministry of Higher Education(MOHE)(missions section)
文摘A metallic nanostructured array that scatters radiation toward a thin metallic layer generates surface plasmon resonances for normally incident light. The location of the minimum of the spectral reflectivity serves to detect changes in the index of refraction of the medium under analysis. The normal incidence operation eases its integration with optical fibers. The geometry of the arrangement and the material selection are changed to optimize some performance parameters as sensitivity, figure of merit, field enhancement, and spectral width. This optimization takes into account the feasibility of the fabrication. The evaluated results of sensitivity(1020 nm/RIU)and figure of merit(614 RIU^(-1)) are competitive with those previously reported.
