One major challenge of using DNA nanostructures for cellular and in vivo applications is their insufficiently structural integrity that stems from the non-covalent base pairing and stacking in complex cellular and phy...One major challenge of using DNA nanostructures for cellular and in vivo applications is their insufficiently structural integrity that stems from the non-covalent base pairing and stacking in complex cellular and physiological environment. The establishment of covalent bonds in DNA nanostructures can link individual strands more stably and therefore should improve the performance of DNA nanostructures in different scenarios where structural integrity is required. Here, we developed a convenient and effective method for constructing covalently stabilized DNA nanostructures by chemically inserting photo-crosslinker(^(CNV)K) in DNA sequences. These covalently linked DNA nanostructures were found to be more resistant to external interference, such as low cation concentrations and unspecific displacement on cell membranes. We also demonstrated that our strategy could improve the efficiency of cell surface receptor-mediated labeling and function regulations in living cells, which sheds light on broadening the biomedical applications of DNA nanostructures.展开更多
Late prenatal growth,early postnatal growth,and layering of the neocortical neurons(NC-Ns)play determining roles in the development of the cerebral cortex(CC).Here,we systematically explore the interactive role of neu...Late prenatal growth,early postnatal growth,and layering of the neocortical neurons(NC-Ns)play determining roles in the development of the cerebral cortex(CC).Here,we systematically explore the interactive role of neuronal surface receptors(NSRs)on cytoskeleton activation(CA)and the piconewton(pN)force generation(P-FG)and their influence on the proper development,growth,and functioning of neurons using a designed DNA nanomechanical device(DNA-NMD).展开更多
基金supported by the National Key Research and Development Program of China (2021YFA0909400)the National Natural Science Foundation of China (21974087, 81974315)+6 种基金Shanghai Municipal Education Commission-Gaofeng Clinical Medicine Grant Support (20181709)Shanghai Rising-Star Program (20QA1405800)the General Projects of China Postdoctoral Fund (2021M692104)Innovative Research Team of High-Level Local Universities in Shanghaifaculty start-up funding support from the Institute of Molecular Medicine of Shanghai Jiao Tong UniversityRecruitment Program of Global Youth Experts of China。
文摘One major challenge of using DNA nanostructures for cellular and in vivo applications is their insufficiently structural integrity that stems from the non-covalent base pairing and stacking in complex cellular and physiological environment. The establishment of covalent bonds in DNA nanostructures can link individual strands more stably and therefore should improve the performance of DNA nanostructures in different scenarios where structural integrity is required. Here, we developed a convenient and effective method for constructing covalently stabilized DNA nanostructures by chemically inserting photo-crosslinker(^(CNV)K) in DNA sequences. These covalently linked DNA nanostructures were found to be more resistant to external interference, such as low cation concentrations and unspecific displacement on cell membranes. We also demonstrated that our strategy could improve the efficiency of cell surface receptor-mediated labeling and function regulations in living cells, which sheds light on broadening the biomedical applications of DNA nanostructures.
基金supported by grants from the National Key R&D Program of China(no.2017YFA0700500)the National Natural Science Foundation of China(no.21635004)the Excellent Research Program of Nanjing University(no.ZYJH004).
文摘Late prenatal growth,early postnatal growth,and layering of the neocortical neurons(NC-Ns)play determining roles in the development of the cerebral cortex(CC).Here,we systematically explore the interactive role of neuronal surface receptors(NSRs)on cytoskeleton activation(CA)and the piconewton(pN)force generation(P-FG)and their influence on the proper development,growth,and functioning of neurons using a designed DNA nanomechanical device(DNA-NMD).
