Recent progress in microbial cell surface display systems and their application on biosensors

Microbial cell surface display technology is a recombinant technology to express target proteins on the cell membrane,which can be used to redesign the cell surface with functional proteins and peptides.Bacterial and yeast surface display systems are the most common cell surface display systems of prokaryotic and eukaryotic proteins,that are widely applied as the core elements in the field of biosensors due to their advantages,including enhanced stability,high yield,good safety,expression of larger and more complex proteins.To further promote the performance of biosensors,the biomineralized microbial surface display technology was proposed.This review summarized the different microbial surface display systems and the biomineralized surface display systems,where the mechanisms of surface display and biomineralization were introduced.Then we described the recent progress of their applications on biosensors for different types of detection targets.Finally,the outlooks and tendencies were discussed and forecasted with the expectation to provide some general functions and enlightenments to this aspect of research.

Progress in Research of Surface-plasmon-enhanced Light-emitting Diode

InGaN-based light-emitting diode(LED)as for the replacement of conventional fluorescent lighting source still needs a great effort to improve the light-extracting efficiency as well as internal quantum efficiency of LEDs.Surface plasmon technology has recently attracted considerable interest,because the spontaneous emission rate and the light extraction efficiency of a light-emitting device can be simultaneously enhanced through the coupling between an InGaN quantum well and surface plasmons.The surface plasmon-emitter coupling technique would lead to high brightness multichip white LEDs that offer realistic alternatives to conventional fluorescent light sources.In this article,the possible enhancement mechanism of surface plasmon is discussed,and then recent developments of surface-plasmon-enhanced light-emitting diode are introduced.

New developments for vacuum coating of steel strips

Vapor deposited coatings onto strip steel are a promising alternative as functional layers for corrosion protection or high abrasion resistance.Conventional coating systems have some limits regarding environmental compatibility,the range of coating materials and application properties. Physical vapor deposition(PVD) is an environment-friendly technology and enables nearly unlimited material and process variety.Electron beam high-rate evaporation with deposition rates up to some micrometers per second is the most productive PVD technology for low cost coating.The combination of evaporation with powerful plasma is an efficient way to improve the layer properties.The developed plasma sources can be used for special plasma enhanced chemical vapor deposition(PECVD) processes too. The paper gives an overview about the latest developments in these technologies.Furthermore,the paper explains some examples of new layer stacks onto steel strips.While enhanced corrosion protection can be obtained by magnesium,aluminium or copper containing coatings other functional surface properties come more and more in the focus of interest.For instance,decorative gold colored layers,transparent scratch resistant layers,hard coatings and photo catalytic layers were deposited on running steel strips.Functional layers and layer systems for energy saving and sun energy absorption by solar thermal effects and photo voltaics are under development.The coatings are prepared under the conditions of very high deposition rates using our in-line vacuum coater for metallic strips and sheets with the name MAXI.The influence of the process and plasma parameters on the layer properties were investigated and will be demonstrated for some applications.

Preparation and characterization of HMX/NH_(2)-GO composite with enhanced thermal safety and desensitization

To improve the safety of HMX,HMX/NH_(2)-GO composite was prepared with aqueous ammonia functionalized graphene oxide(NH_(2)-GO).The composite was characterized by SEM,Zeta potential,XPS,Raman spectrum,XRD,HPLC,DSC and BAM sensitivity test.The results indicated that the functionalization with aqueous ammonia can enhance the interaction between GO and HMX,and more efficiently desensitize the explosive.The optimal impact sensitivity of the HMX/NH_(2)-GO composite can be not less than 40 J,which is also the most insensitivity compared to the previous reports prepared by coating desensitization with non-energetic desensitized material.Moreover,the potential reason for the different impact and friction sensitivity was also discussed,which may bring a novel perspective to achieve the desensitization of energetic material.

Isolation of Goose-origin scFv Antibodies Against Goose Parvovirus from Bacterial Display Antibody Libraries

Goose parvovirus(GPV)can cause a highly contagious and fatal gosling plague(GP)disease in goslings and muscoy ducklings.Here,three goose-origin neutralizing single chain variable fragment(scFv)antibodies against GPV SYG-61 were isolated.The genes of scFv antibodies were derived from goslings immunized with GPV SYG-61,and scFvs were subcloned into a pBSD vector for the construction of pBSD-scFv libraries.The pBSD-scFv libraries were screened following three rounds using VP2(protective antigen of GPV)as the bait by flow cytometry(FCM).After screening,the 15 clones with high mean fluorescence intensity(MFI)were isolated and sequenced.These 15 scFvs were expressed by pET-28a(+)in E.coli.The specificity and affinity of the 15 purified scFvs were successfully confirmed by ELISA.In the preliminary neutralization experiment on primary goose embryo fibroblast(GEF)in vitro,three of the 15 purified scFvs(named scFv-10,scFv-11 and scFv-50)showed significant neutralizing capacities.The study generated the first goose-origin neutralizing scFv against GPV and laid the foundation for the appearance of full-length goose-origin neutralizing monoclonal antibody against GPV.

Load Optimization Scheduling of Chip Mounter Based on Hybrid Adaptive Optimization

A chip mounter is the core equipment in the production line of the surface-mount technology,which is responsible for finishing the mount operation.It is the most complex and time-consuming stage in the production process.Therefore,it is of great significance to optimize the load balance and mounting efficiency of the chip mounter and improve the mounting efficiency of the production line.In this study,according to the specific type of chip mounter in the actual production line of a company,a maximum and minimum model is established to minimize the maximum cycle time of the chip mounter in the production line.The production efficiency of the production line can be improved by optimizing the workload scheduling of each chip mounter.On this basis,a hybrid adaptive optimization algorithm is proposed to solve the load scheduling problem of the mounter.The hybrid algorithm is a hybrid of an adaptive genetic algorithm and the improved ant colony algorithm.It combines the advantages of the two algorithms and improves their global search ability and convergence speed.The experimental results show that the proposed hybrid optimization algorithm has a good optimization effect and convergence in the load scheduling problem of chip mounters.