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Intracellular detection of cytokine expression with four-color flow cytometry in pregnant NOD/SCID mice
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作者 YI LIN ZHEN HONG LIANG ZHENG FENG YAO YING ZENG 《Journal of Microbiology and Immunology》 2005年第3期199-205,共7页
The aim of this study was to evaluate the relationship between the embryo resorption rate (RR) and the status of local immunity at the feto-maternal interface in pregnant NOD/SCID mice. RR was calculated in NOD/SCID... The aim of this study was to evaluate the relationship between the embryo resorption rate (RR) and the status of local immunity at the feto-maternal interface in pregnant NOD/SCID mice. RR was calculated in NOD/SCID x NOD/SCID mice and compared with non-immunodeficiency BALB/c × BALB/c mice on day 13.5 of gestation. Intraeellular detection of cytokine expression was performed with four-color flow cytometry to identify the functional subsets of lymphocytes in NOD/SCID mice before being bred or during pregnancy. No statistically supported difference in RRs was observed between NOD/SCID × NOD/SCID and control BALB/c × BALB/c mice. Accordingly, although multiple immunodeficits were confirmed in NOD/SCID mice, the percentages of several functional cell subsets were spontaneously altered during pregnancy, and this may be correlated with the roughly normal pregnancy outcomes observed in these mice. The spontaneous alteration of cell percentages at the feto-maternal interfaee in NOD/SCID mice may be of benefit to the pregnancy outcomes. 展开更多
关键词 Animal model Cell surface marker Immunodeficiency Pregnancy tolerance
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Influence of substrate process tolerances on transmission characteristics of frequency-selective surface 被引量:7
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作者 张鹤 卢俊 +1 位作者 孙贯成 肖洪亮 《Chinese Optics Letters》 SCIE EI CAS CSCD 2008年第1期54-56,共3页
Frequency-selective surface (FSS) is a two-dimensional periodic structure consisting of a dielectric substrate and the metal units (or apertures) arranged periodically on it. When manufacturing the substrate, its ... Frequency-selective surface (FSS) is a two-dimensional periodic structure consisting of a dielectric substrate and the metal units (or apertures) arranged periodically on it. When manufacturing the substrate, its thickness and dielectric constant suffer process tolerances. This may induce the center frequency of the FSS to shift, and consequently influence its characteristics. In this paper, a bandpass FSS structure is designed. The units are the Jerusalem crosses arranged squarely. The mode-matching technique is used for simulation. The influence of the tolerances of the substrate's thickness and dielectric constant on the center frequency is analyzed. Results show that the tolerances of thickness and dielectric constant have different influences on the center frequency of the FSS. It is necessary to ensure the process tolerance of the dielectric constant in the design and manufacturing of the substrate in order to stabilize the center frequency. 展开更多
关键词 FSS Influence of substrate process tolerances on transmission characteristics of frequency-selective surface
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