The central buckle, which is often used in a suspension bridge, can improve bridges' performance in the actual operation condition. The influence of the central buckle on natural vibration characteristics and brid...The central buckle, which is often used in a suspension bridge, can improve bridges' performance in the actual operation condition. The influence of the central buckle on natural vibration characteristics and bridge-deck driving comfort of a long-span suspension bridge is studied by using a case study of Siduhe Suspension Bridge in China. Based on the finite element software ANSYS and independently complied program, the influence of the central buckle on the structure force-applied characteristics of a long-span suspension bridge has been explored. The results show that the huge increases of natural frequencies can result in the presence of central buckles because of the increases of bending and torsional rigidities. The central buckle basically makes the stiffening girders and cables within the triangular area covered as a relatively approximate rigid area. Hence, the central buckle can reduce the torsional displacement of the main girder. However, the increases of bending and torsional rigidities have little influence on the impact factor, which is obtained by using vehicle-bridge coupled vibration analysis. This means that the central buckle has little effect on the comfort indices. In addition, it is found that the central buckle can enhance the bridge deck's driving stability due to the decrease of the torsional displacements of the main girder.展开更多
In order to clarify the charging characteristics of suspension droplets in ion flow field under different temperatures and humidity,the effective charging factor used to characterize the charging characteristics of su...In order to clarify the charging characteristics of suspension droplets in ion flow field under different temperatures and humidity,the effective charging factor used to characterize the charging characteristics of suspension droplets is introduced in this paper,and a calculation method of charging factor is proposed based on the upstream finite element method(FEM).Then,the charging factor under different temperatures and humidity is calculated,and the analytic expression of the charging factor considering the influence of temperature and humidity is obtained by fitting the calculation results.The influence of suspension droplets on the ion flow field is analyzed.The results show that the charging factor is small and increases little with the relative humidity when the relative humidity is less than 60%,and the charging factor is large and increases rapidly with the relative humidity when the relative humidity is more than 60%.At the same relative humidity,the charging factor increases linearly with the temperature.The influence of charged suspension droplets on the ion flow field can be ignored when the relative humidity is less than 60%and must be considered under high temperature and humidity.The calculation method and analytic expression of the charging factor proposed in this paper can be used to model of ion flow field considering the influence of temperature and humidity and provide technical support for the construction of HVDC transmission lines across high temperature and humidity.展开更多
To improve the suspension performance and steering stability of light vehicles, we built a kinematic simulation model of a whole independent double-wishbone suspension system by using ADAMS software, created random ex...To improve the suspension performance and steering stability of light vehicles, we built a kinematic simulation model of a whole independent double-wishbone suspension system by using ADAMS software, created random excitations of the test platforms of respectively the left and the right wheels according to actual running conditions of a vehicle, and explored the changing patterns of the kinematic characteristic parameters in the process of suspension motion. The irrationality of the suspension guiding mechanism design was pointed out through simulation and analysis, and the existent problems of the guiding mechanism were optimized and calculated. The results show that all the front-wheel alignment parameters, including the camber, the toe, the caster and the inclination, only slightly change within corresponding allowable ranges in design before and after optimization. The optimization reduces the variation of the wheel-center distance from 47.01 mm to a change of 8.28 mm within the allowable range of ?10 mm to 10 mm, promising an improvement of the vehicle steering stability. The optimization also confines the front-wheel sideways slippage to a much smaller change of 2.23 mm; this helps to greatly reduce the wear of tires and assure the straight running stability of the vehicle.展开更多
Based on the theory and the practical experiences of linearity design of feasible design area and inverse solution of non linear outer characteristic of suspension shock absorber, in accordance with non linearity ou...Based on the theory and the practical experiences of linearity design of feasible design area and inverse solution of non linear outer characteristic of suspension shock absorber, in accordance with non linearity outer characteristic formed by open up damping coefficient, full open damping coefficient and smoothness to safety ratio of suspension shock absorber, a method and a research conclusion of the feasible design and inverse solution for the basic problems of designing and inverse solution of non linear outer characteristic of suspension damping components are provided.展开更多
Multi-pylon multi-span suspension bridge is a new type super flexible structure system, and the rigidity design of middle pylon is one of the main difficult technical issues. Due to the requirements of longitudinal ri...Multi-pylon multi-span suspension bridge is a new type super flexible structure system, and the rigidity design of middle pylon is one of the main difficult technical issues. Due to the requirements of longitudinal rigidity, the structural form and the corresponding foundation type of middle pylon are different from those of the ordinary steel pylon, and the complicated dynamic characteristics make the calculation quite difficult. In this article, exploration has been made in selection of similarity ratio and model materials, section simulation, restriction conditions simulation, fixing of mass blocks, fabrication scheme and testing method by taking into account different construction and working conditions such as restriction conditions and working environment of a three-pylon suspension bridge, to conduct the test experimental design of the dynamic behavior of the middle pylon, with the purpose to reveal its dynamic characteristics and make comparison and analysis with theoretical assumptions, to provide basis for anti-wind and anti-seismic design and reference for the design and research of three-pylon two-span suspension bridges in the future.展开更多
本研究通过筛选驯化的MDCK细胞获得无血清悬浮生长MDCK细胞(命名为MDCK-sus),测定该细胞的比生长速率、细胞活力及细胞最大生长密度等生长特性,采用间接免疫荧光法和流式细胞仪检测法比较母本MDCK细胞与MDCK-sus细胞流感病毒受体[唾液酸...本研究通过筛选驯化的MDCK细胞获得无血清悬浮生长MDCK细胞(命名为MDCK-sus),测定该细胞的比生长速率、细胞活力及细胞最大生长密度等生长特性,采用间接免疫荧光法和流式细胞仪检测法比较母本MDCK细胞与MDCK-sus细胞流感病毒受体[唾液酸-α-2,3-半乳糖糖链受体(SAα2,3Gal)和唾液酸-α-2,6-半乳糖糖链受体(SAα2,6Gal)]的丰度,用荧光定量PCR检测比较细胞中α-2,3唾液酸转移酶(ST3Gals)基因与α-2,6唾液酸转移酶(ST6Gals)基因表达水平的差异,通过测定流感病毒血凝HA效价比较母本MDCK细胞与MDCK-sus细胞对流感病毒的增殖能力。结果表明,经驯化获得的适合无血清悬浮培养的MDCK-sus细胞,细胞密度最高可达1ml 3.6×106个细胞,最大比生长速率可达1 d 0.56。MDCK-sus细胞表面流感病毒受体SAα2,3Gal的丰度明显高于母体MDCK细胞,ST3Gal转移酶基因表达水平也明显高于母本MDCK细胞。MDCK细胞驯化后增殖禽流感病毒的能力增强,其中H9亚型禽流感病毒AH1102株在MDCK-sus细胞中HA效价达到9lg2(25μl)。MDCK-sus细胞各种生长特性表明,其适于H9亚型禽流感的繁殖,可以为采用悬浮细胞培养禽流感疫苗的大规模工业化生产提供技术支持。展开更多
基金Project(2015CB057701)supported by the National Basic Research Program of ChinaProjects(51308071,51378081)supported by the National Natural Science Foundation of China+2 种基金Project(3JJ4057)supported by the Natural Science Foundation of Hunan Province,ChinaProject(12K076)supported by the Open Fund of Innovation Platform in Hunan Provincial Universities,ChinaProject(2015319825120)supported by the Traffic Department of Appliced Basic Research,China
文摘The central buckle, which is often used in a suspension bridge, can improve bridges' performance in the actual operation condition. The influence of the central buckle on natural vibration characteristics and bridge-deck driving comfort of a long-span suspension bridge is studied by using a case study of Siduhe Suspension Bridge in China. Based on the finite element software ANSYS and independently complied program, the influence of the central buckle on the structure force-applied characteristics of a long-span suspension bridge has been explored. The results show that the huge increases of natural frequencies can result in the presence of central buckles because of the increases of bending and torsional rigidities. The central buckle basically makes the stiffening girders and cables within the triangular area covered as a relatively approximate rigid area. Hence, the central buckle can reduce the torsional displacement of the main girder. However, the increases of bending and torsional rigidities have little influence on the impact factor, which is obtained by using vehicle-bridge coupled vibration analysis. This means that the central buckle has little effect on the comfort indices. In addition, it is found that the central buckle can enhance the bridge deck's driving stability due to the decrease of the torsional displacements of the main girder.
基金supported by National Natural Science Foundation of China(No.52077074)。
文摘In order to clarify the charging characteristics of suspension droplets in ion flow field under different temperatures and humidity,the effective charging factor used to characterize the charging characteristics of suspension droplets is introduced in this paper,and a calculation method of charging factor is proposed based on the upstream finite element method(FEM).Then,the charging factor under different temperatures and humidity is calculated,and the analytic expression of the charging factor considering the influence of temperature and humidity is obtained by fitting the calculation results.The influence of suspension droplets on the ion flow field is analyzed.The results show that the charging factor is small and increases little with the relative humidity when the relative humidity is less than 60%,and the charging factor is large and increases rapidly with the relative humidity when the relative humidity is more than 60%.At the same relative humidity,the charging factor increases linearly with the temperature.The influence of charged suspension droplets on the ion flow field can be ignored when the relative humidity is less than 60%and must be considered under high temperature and humidity.The calculation method and analytic expression of the charging factor proposed in this paper can be used to model of ion flow field considering the influence of temperature and humidity and provide technical support for the construction of HVDC transmission lines across high temperature and humidity.
基金the Postdoctoral Science Foundation of China (No. 2004036396)the Foundation of 985- Automotive Engineering of Jilin University
文摘To improve the suspension performance and steering stability of light vehicles, we built a kinematic simulation model of a whole independent double-wishbone suspension system by using ADAMS software, created random excitations of the test platforms of respectively the left and the right wheels according to actual running conditions of a vehicle, and explored the changing patterns of the kinematic characteristic parameters in the process of suspension motion. The irrationality of the suspension guiding mechanism design was pointed out through simulation and analysis, and the existent problems of the guiding mechanism were optimized and calculated. The results show that all the front-wheel alignment parameters, including the camber, the toe, the caster and the inclination, only slightly change within corresponding allowable ranges in design before and after optimization. The optimization reduces the variation of the wheel-center distance from 47.01 mm to a change of 8.28 mm within the allowable range of ?10 mm to 10 mm, promising an improvement of the vehicle steering stability. The optimization also confines the front-wheel sideways slippage to a much smaller change of 2.23 mm; this helps to greatly reduce the wear of tires and assure the straight running stability of the vehicle.
文摘Based on the theory and the practical experiences of linearity design of feasible design area and inverse solution of non linear outer characteristic of suspension shock absorber, in accordance with non linearity outer characteristic formed by open up damping coefficient, full open damping coefficient and smoothness to safety ratio of suspension shock absorber, a method and a research conclusion of the feasible design and inverse solution for the basic problems of designing and inverse solution of non linear outer characteristic of suspension damping components are provided.
文摘Multi-pylon multi-span suspension bridge is a new type super flexible structure system, and the rigidity design of middle pylon is one of the main difficult technical issues. Due to the requirements of longitudinal rigidity, the structural form and the corresponding foundation type of middle pylon are different from those of the ordinary steel pylon, and the complicated dynamic characteristics make the calculation quite difficult. In this article, exploration has been made in selection of similarity ratio and model materials, section simulation, restriction conditions simulation, fixing of mass blocks, fabrication scheme and testing method by taking into account different construction and working conditions such as restriction conditions and working environment of a three-pylon suspension bridge, to conduct the test experimental design of the dynamic behavior of the middle pylon, with the purpose to reveal its dynamic characteristics and make comparison and analysis with theoretical assumptions, to provide basis for anti-wind and anti-seismic design and reference for the design and research of three-pylon two-span suspension bridges in the future.
文摘本研究通过筛选驯化的MDCK细胞获得无血清悬浮生长MDCK细胞(命名为MDCK-sus),测定该细胞的比生长速率、细胞活力及细胞最大生长密度等生长特性,采用间接免疫荧光法和流式细胞仪检测法比较母本MDCK细胞与MDCK-sus细胞流感病毒受体[唾液酸-α-2,3-半乳糖糖链受体(SAα2,3Gal)和唾液酸-α-2,6-半乳糖糖链受体(SAα2,6Gal)]的丰度,用荧光定量PCR检测比较细胞中α-2,3唾液酸转移酶(ST3Gals)基因与α-2,6唾液酸转移酶(ST6Gals)基因表达水平的差异,通过测定流感病毒血凝HA效价比较母本MDCK细胞与MDCK-sus细胞对流感病毒的增殖能力。结果表明,经驯化获得的适合无血清悬浮培养的MDCK-sus细胞,细胞密度最高可达1ml 3.6×106个细胞,最大比生长速率可达1 d 0.56。MDCK-sus细胞表面流感病毒受体SAα2,3Gal的丰度明显高于母体MDCK细胞,ST3Gal转移酶基因表达水平也明显高于母本MDCK细胞。MDCK细胞驯化后增殖禽流感病毒的能力增强,其中H9亚型禽流感病毒AH1102株在MDCK-sus细胞中HA效价达到9lg2(25μl)。MDCK-sus细胞各种生长特性表明,其适于H9亚型禽流感的繁殖,可以为采用悬浮细胞培养禽流感疫苗的大规模工业化生产提供技术支持。
