We prepared graphene（GE） with a mean size of 3087 nm.The transition of graphene oxide（GO） to GE was confirmed by UV-visible spectroscopy,Fourier transform infrared spectroscopy（FTIR） and X-ray diffraction（XRD）...We prepared graphene（GE） with a mean size of 3087 nm.The transition of graphene oxide（GO） to GE was confirmed by UV-visible spectroscopy,Fourier transform infrared spectroscopy（FTIR） and X-ray diffraction（XRD）.The experimental results of optical microscopic observation indicated that the GE ranged from 5 to 20 μg/mL did not affect the cell morphologies of the PC12 cells.The results of cell viability and membrane integrity assay supported that of optical microscopic observation and demonstrated that the GE ranged from 5 to 20 μg/mL presented no obvious cytotoxicity.However,reactive oxygen species（ROS） assay suggested that an elevation of ROS level could be detected when the GE ranged from 20 to 100 μg/mL.These results showed that the GE ranged from 5 to 10 μg/mL presented an excellent in vitro biocompatibility and was one kind of potential biomaterials for neural tissue engineering.展开更多
基金Funded by the Natural Science Foundation of Hubei Province(No.2014CFB839)the Doctoral Research Fund of Wuhan University of Technology(No.471-40120093)+4 种基金the Opening Project of Jiangsu Provincial Key Laboratory of Silk Engineering(No.KJS1415)the Hong Kong,Macao and Taiwan Science&Technology Cooperation Program of China(No.2015DFH30180)the Fundamental Research Funds for the Central Universities(No.WUT:2014-Ⅶ-028)the National Natural Science Foundation of China(No.51403168)the Key Project of Science and Technology of Wuhan(No.2014060202010120)
文摘We prepared graphene（GE） with a mean size of 3087 nm.The transition of graphene oxide（GO） to GE was confirmed by UV-visible spectroscopy,Fourier transform infrared spectroscopy（FTIR） and X-ray diffraction（XRD）.The experimental results of optical microscopic observation indicated that the GE ranged from 5 to 20 μg/mL did not affect the cell morphologies of the PC12 cells.The results of cell viability and membrane integrity assay supported that of optical microscopic observation and demonstrated that the GE ranged from 5 to 20 μg/mL presented no obvious cytotoxicity.However,reactive oxygen species（ROS） assay suggested that an elevation of ROS level could be detected when the GE ranged from 20 to 100 μg/mL.These results showed that the GE ranged from 5 to 10 μg/mL presented an excellent in vitro biocompatibility and was one kind of potential biomaterials for neural tissue engineering.
