Sucrose non-fermenting-1-related protein kinase-1(SnRK1)regulates carbon and nitrogen metabolism in plants.However,its roles and their underlying mechanisms in tolerance to abiotic stresses are little known.The presen...Sucrose non-fermenting-1-related protein kinase-1(SnRK1)regulates carbon and nitrogen metabolism in plants.However,its roles and their underlying mechanisms in tolerance to abiotic stresses are little known.The present study indicated that the IbSnRK1 gene was strongly induced by Na Cl,polyethylene glycol(PEG)6000,hydrogen peroxide(H2O2),cold(4°C),and abscisic acid(ABA).Its overexpression significantly increased salt,drought,and cold tolerance in transgenic sweet potato plants.ABA,proline,and K+contents were significantly increased,whereas malondialdehyde(MDA),Na+and H2O2 contents and O2-production rate were significantly decreased in the transgenic plants under salt,drought,and cold stresses.Overexpression of the gene up-regulated genes involved in ABA biosynthesis,stress response,and stomatal closure;increased enzyme activities in the reactive oxygen species(ROS)scavenging system;and controlled stomatal closure under salt,drought,and cold stresses.These results show that the IbSnRK1 gene confers salt,drought,and cold tolerance in sweet potato by activating the ROS scavenging system and controlling stomatal closure via the ABA signaling pathway.展开更多
The aim of this study was to investigate the effect of different concentrations of sporamin on the differentiation and proliferation of 3T3-LI preadipocytes, providing the theoretical basis for the development of food...The aim of this study was to investigate the effect of different concentrations of sporamin on the differentiation and proliferation of 3T3-LI preadipocytes, providing the theoretical basis for the development of food to treat obesity and diabetes, The isolation and purification of sporamin from sweet potato species 55-2 were performed by ammonium sulphate precipitation in combination with ion-exchange and gel filtration chromatography. With berberine as a positive control, different concentrations ofsporamin （0.000, 0.125, 0.025, 0.250, 0.500, and 1.000 mg·mL^-1 were used to treat 3T3-L1 preadipocytes. Intracellular fat accumulation and the degree of adipogenesis were quantified using Oil Red O staining and colorimetry, Preadipocytes differentiation was measured by 3（4,5-dimethylthiazolyl-2-yl）-2,5-diphenyltetrazolium bromide （MTT） spectrophotometric assay. Two sporamin proteins, which were separated into sporamin A （31 kD） and sporamin B （22 kD）, could be purified by ion-exchange and gel filtration chromatography. After being treated by different concentrations of sporamin, the differentiation of 3T3-L1 preadipocytes was significantly inhibited, compared with the positive control. When the sporamin solution concentration was 0.500 mg mL-1, the accumulation of lipid droplets within the cells was significantly decreased and the optical density （OD） value of the solution from destained Oil Red O reached to 0.35, which was the lowest value （P〈 0.05）. The proliferation of 3T3-L1 preadipocytes was significantly inhibited by treating at higher sporamin concentrations. In addition, the inhibitory effect was more obvious with the prolonged treatment time （P〈 0.05）. The differentiation and proliferation of 3T3-L1 preadipocytes could be inhibited significantly by the addition of higher concentration sporamin. It was, therefore, suggested that the sporamin was potentially effective for weight loss.展开更多
基金supported by the National Key Research and Development Program of China(2019YFD1001303 and 2019YFD1001300)China Agriculture Research System(CARS-10,Sweetpotato)。
文摘Sucrose non-fermenting-1-related protein kinase-1(SnRK1)regulates carbon and nitrogen metabolism in plants.However,its roles and their underlying mechanisms in tolerance to abiotic stresses are little known.The present study indicated that the IbSnRK1 gene was strongly induced by Na Cl,polyethylene glycol(PEG)6000,hydrogen peroxide(H2O2),cold(4°C),and abscisic acid(ABA).Its overexpression significantly increased salt,drought,and cold tolerance in transgenic sweet potato plants.ABA,proline,and K+contents were significantly increased,whereas malondialdehyde(MDA),Na+and H2O2 contents and O2-production rate were significantly decreased in the transgenic plants under salt,drought,and cold stresses.Overexpression of the gene up-regulated genes involved in ABA biosynthesis,stress response,and stomatal closure;increased enzyme activities in the reactive oxygen species(ROS)scavenging system;and controlled stomatal closure under salt,drought,and cold stresses.These results show that the IbSnRK1 gene confers salt,drought,and cold tolerance in sweet potato by activating the ROS scavenging system and controlling stomatal closure via the ABA signaling pathway.
基金supported by the National 863 Program of China (2006AA10Z332)the Ear Marked Fund for Modern Agro-Industry Technology Research System, China
文摘The aim of this study was to investigate the effect of different concentrations of sporamin on the differentiation and proliferation of 3T3-LI preadipocytes, providing the theoretical basis for the development of food to treat obesity and diabetes, The isolation and purification of sporamin from sweet potato species 55-2 were performed by ammonium sulphate precipitation in combination with ion-exchange and gel filtration chromatography. With berberine as a positive control, different concentrations ofsporamin （0.000, 0.125, 0.025, 0.250, 0.500, and 1.000 mg·mL^-1 were used to treat 3T3-L1 preadipocytes. Intracellular fat accumulation and the degree of adipogenesis were quantified using Oil Red O staining and colorimetry, Preadipocytes differentiation was measured by 3（4,5-dimethylthiazolyl-2-yl）-2,5-diphenyltetrazolium bromide （MTT） spectrophotometric assay. Two sporamin proteins, which were separated into sporamin A （31 kD） and sporamin B （22 kD）, could be purified by ion-exchange and gel filtration chromatography. After being treated by different concentrations of sporamin, the differentiation of 3T3-L1 preadipocytes was significantly inhibited, compared with the positive control. When the sporamin solution concentration was 0.500 mg mL-1, the accumulation of lipid droplets within the cells was significantly decreased and the optical density （OD） value of the solution from destained Oil Red O reached to 0.35, which was the lowest value （P〈 0.05）. The proliferation of 3T3-L1 preadipocytes was significantly inhibited by treating at higher sporamin concentrations. In addition, the inhibitory effect was more obvious with the prolonged treatment time （P〈 0.05）. The differentiation and proliferation of 3T3-L1 preadipocytes could be inhibited significantly by the addition of higher concentration sporamin. It was, therefore, suggested that the sporamin was potentially effective for weight loss.
