AIM: To investigate the effects of proteins purified from sweet potato storage roots on human colorectal cancer cell lines. METHODS: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, Hoechst 33...AIM: To investigate the effects of proteins purified from sweet potato storage roots on human colorectal cancer cell lines. METHODS: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, Hoechst 33258 nuclear staining and Boyden transwell chamber methods were used to determine whether purified sweet potato protein (SPP) from fresh sweet potato roots affected proliferation, migration and invasion, respectively, of human colorectal cancer SW480 cells in vitro . The inhibitory effects of SPP on growth of human colorectal cancer HCT-8 cells intraperitoneally xenografted in nude mice and spontaneous lung metastasis of murine Lewis lung carcinoma 3LL cells subcutaneously transplanted in C57 BL/6 mice were also investigated in vivo . RESULTS: SPP inhibited the proliferation of SW480 cells in a dose-dependent manner, with an IC 50 value of 38.732 μmol/L (r2 = 0.980, P = 0.003) in the MTT assay. Hoechst 33258 nuclear staining further revealed inhibition of cell viability and induction of apoptosis by SPP. The transwell assay disclosed significant reduction in migrated cells/field by 8 μmol/L SPP (8.4 ± 2.6 vs 23.3 ± 5.4, P = 0.031) and invaded cells/field through the ECMatrix by 0.8 μmol/L SPP, compared with the control (25.2 ± 5.2 vs 34.8 ± 6.1, P = 0.038). Both intraperitoneal (ip) and intragastric (ig) administration of SPP led to significant suppression of growth of intraperitoneally inoculated HCT-8 cells in nude mice to 58.0% ± 5.9% (P = 0.037) and 43.5% ± 7.1% (P = 0.004) of the controls, respectively, after 9 d treatment. Bloody ascites additionally disappeared after ip injection of trypsin inhibitor. Notably, ig and ip administration of SPP induced a significant decrease in spontaneous pulmonary metastatic nodule formation in C57 BL/6 mice (21.0 ± 12.3 and 27.3 ± 12.7 nodules/lung vs 42.5 ± 4.5 nodules/lung in controls, respectively, P < 0.05) after 25 d treatment. Moreover, the average weight of primary tumor nodules in the hind leg of mice decreased from 8.2 ± 1.3 g/mice in the control to 6.1 ± 1.4 g/mice in the ip group (P = 0.035). CONCLUSION: SPP exerts significant antiproliferative and antimetastatic effects on human colorectal cancer cell lines, both in vitro and in vivo .展开更多
Some indices concerning the metabolism of substance and energy in sweet potato leaves under water stress were studied. The results showed an obvious increase in soluble protein content. Compared with control, Chl a, C...Some indices concerning the metabolism of substance and energy in sweet potato leaves under water stress were studied. The results showed an obvious increase in soluble protein content. Compared with control, Chl a, Chl b, total Chl contents and the ratio of Chl a to Chl b all decreased to some extent. ATP content increased in some varieties and decreased in others, but the stronger the drought resistance of the variety , the higher the ATP content. The correlation coefficient(r)of the soluble protein content, ratio of Chl a to Chl b and ATP content as a percentage of the drought-resistant sweet potato control variety are 0. 8968, - 0. 8509 and 0. 8200, respectively, P<0. 01. So these indices can be used to evaluate the drought resistance of different sweet potato varieties.展开更多
Despite being a healthy low glycaemic index food, the production and consumption of sweet potatoes are decreasing globally. The global production trends indicated a decline from 130.47 million tonnes (MT) in 2004 to 1...Despite being a healthy low glycaemic index food, the production and consumption of sweet potatoes are decreasing globally. The global production trends indicated a decline from 130.47 million tonnes (MT) in 2004 to 107.64 MT in 2009, while the production in China, having the largest share towards world production of sweet potatoes, witnessed a decrease from 105.84 MT in 2004 to 81.21 MT in 2009. There is an interest in increasing consumption of healthy low glycaeimic index foods, especially in the context of a projected alarming rise in the diabetic population in the developing world by 2025. The objective of this study was to enhance the utilization of sweet potato as a low glycaemic food, mainly through its use in the development of high protein pasta. Among three protein sources, whey protein concentrate (WPC), defatted soy flour (DSF), and fish powder (FP), WPC gave high quality pasta with strong starch-protein network formation, as evidenced from scanning electron microscopic studies and low in vitro starch digestibility. Protein nutritional quality was also high for WPC-fortified sweet potato pasta, with very high scores for lysine and leucine as well as high essential amino acid index and calculated protein efficiency ratio. Fractionation of starch showed that the WPC-fortified sweet potato pasta had the lowest rapidly digested starch (RDS) and the highest resistant starch (RS) content, indicating its potential as a low glycaemic food.展开更多
Sweet potato is one of the first natural GMOs, genetically modified 8000 years ago by Agrobacterium rhizogenes as reported recently by Kyndt et al. A section of 10 kbp long DNA (Transferred- DNA or T-DNA) of the Ri (R...Sweet potato is one of the first natural GMOs, genetically modified 8000 years ago by Agrobacterium rhizogenes as reported recently by Kyndt et al. A section of 10 kbp long DNA (Transferred- DNA or T-DNA) of the Ri (Root-inducing) plasmid was transferred to the plant genome by A. rhizo-genes and has been maintained in all 291 hexaploid sweet potato cultivars of the world. The maintenance in the sweet potato genome and expression of two T-DNA genes for tryptophan-2-monooxygenease (iaaM) and for indole-3-acetamide hydrolase (iaaH) are likely to be physiologically significant since these enzymes convert tryptophan to indole-3-acetic acid, a major plant growth hormone auxin. Sweet potato (Ipomoea batatas (L.) Lam) is ranked the third most important root crop after potato and cassava, and the seventh in global food crop production with more than 126 million metric tons. Although sweet potato originated in Central or South America, China currently produces over 86% of world production with 109 million metric tons. In the United States, North Carolina is the leading producer with 38.5% of the 2007 sweet potato production, followed by California, Mississippi, and Louisiana with 23%, 19%, and 15.9%, respectively. Leaf curl virus diseases have been reported in sweet potato throughout the world. One of the causal agents is Sweet potato leaf curl virus (SPLCV) belonging to the genus Begomovirus (family Geminiviridae). Although SPLCV does not cause symptoms on Beauregard, one of the most predominant sweet potato cultivars in the US, it can reduce the yield up to 26%. Serological detection of SPLCV is not currently available due to the difficulties in obtaining purified virions that can be used as antigen for antiserum production. In attempts to obtain the coat protein (CP) of SPLCV for antibody production, primers were designed to amplify the CP gene. This gene was cloned into the expression vector pMAL-c2E as a fusion protein with maltose-binding protein, and transformed into Escherichia coli strain XL1-Blue. After gene induction, a fusion protein of 72 kDa was purified by amylose affinity chromatography. The yield of the purified fusion protein was approximately 200 μg/liter of bacterial culture. Digestion with enterokinase cleaved the fusion protein into a 42.5 kDa maltosebinding protein and a 29.4 kDa protein. The latter protein was identified by mass spectrometry analysis as the coat protein of SPLCV based on the fact that the mass spectrometry elucidated the sequences corresponding to 37% of amino acid positions of the SPLCV coat protein.展开更多
为探究蛋白质和淀粉对魔芋凝胶的影响,研究不同质量分数红薯淀粉(sweet potato starch,SPS)和大豆分离蛋白(soy protein isolate,SPI)对魔芋凝胶的质地、色泽、感官、水分分布、流变学特性和微观结构的影响。单因素试验结果表明,SPS质...为探究蛋白质和淀粉对魔芋凝胶的影响,研究不同质量分数红薯淀粉(sweet potato starch,SPS)和大豆分离蛋白(soy protein isolate,SPI)对魔芋凝胶的质地、色泽、感官、水分分布、流变学特性和微观结构的影响。单因素试验结果表明,SPS质量分数从0%增加到2.5%对魔芋凝胶质构特性有显著影响(P<0.05),硬度和弹性分别上升86.43%和27.69%,凝胶强度和持水性显著上升(P<0.05);添加2%以下的SPI显著提高凝胶的L*值和b*值(P<0.05),硬度和弹性分别上升13.41%和15.38%,凝胶强度和持水性显著上升(P<0.05),但胶黏性降低54.19%。流变学分析结果表明,随着SPS和SPI质量分数增加,凝胶G’和G”值逐渐上升,表明凝胶结构更加牢固。低场核磁共振结果表明,二者的添加显著增加了凝胶体系中结合水含量(P<0.05),自由水和弛豫时间没有显著变化。综合评分实验结果表明,优化配比后的复合凝胶质构特性进一步提高。扫描电子显微镜结果表明,优化配比后的复合凝胶形成更为致密、结构连续的稳定结构。研究表明添加2.5%SPS和1.0%SPI为复合凝胶的最佳配比。总体来说,本研究结果为魔芋凝胶在方便即食食品的应用提供良好的实验参考和理论基础。展开更多
以‘紫罗兰’紫薯为原料,研究同步提取紫薯花青素以及制备紫薯淀粉、纤维素和紫薯蛋白的工艺及参数。紫薯与酸乙醇(p H 2)混合破碎、过滤、沉淀分离淀粉;将滤渣和分离淀粉后上清液混合,用微波辅助法提取花青素并优化提取条件;花青素提...以‘紫罗兰’紫薯为原料,研究同步提取紫薯花青素以及制备紫薯淀粉、纤维素和紫薯蛋白的工艺及参数。紫薯与酸乙醇(p H 2)混合破碎、过滤、沉淀分离淀粉;将滤渣和分离淀粉后上清液混合,用微波辅助法提取花青素并优化提取条件;花青素提取液沉淀分离紫薯蛋白;提取花青素的滤渣制备紫薯纤维素。结果表明:微波辅助提取紫薯中花青素的最佳工艺条件为微波时间4 min、微波温度52℃、料液比1∶22.40(g/m L)、乙醇体积分数62%(p H 2),在此条件下紫薯花青素的提取率(93.64±0.69)%、粗提物中花青素含量(9.58±0.20)mg/g。制备的紫薯淀粉质量分数(95.77±0.41)%、得率(占总淀粉质量分数)(73.06±1.03)%;滤渣粉中纤维素含量(117.11±2.69) mg/g;制备的紫薯蛋白中蛋白质含量(524.78±24.84) mg/g。该制备方法能够提高紫薯的利用率,降低生产成本。展开更多
采用硫酸铵沉淀法制备紫薯花色苷-蛋白复合物,经二乙氨基乙基纤维素52和Sephadex G-75葡聚糖凝胶纯化得紫薯纯蛋白,利用聚丙烯酰胺凝胶电泳测定紫薯蛋白及紫薯花色苷-蛋白复合物的分子质量,运用紫外、荧光、红外和圆二色谱多种光谱方法...采用硫酸铵沉淀法制备紫薯花色苷-蛋白复合物,经二乙氨基乙基纤维素52和Sephadex G-75葡聚糖凝胶纯化得紫薯纯蛋白,利用聚丙烯酰胺凝胶电泳测定紫薯蛋白及紫薯花色苷-蛋白复合物的分子质量,运用紫外、荧光、红外和圆二色谱多种光谱方法对紫薯蛋白及紫薯花色苷-蛋白复合物进行结构表征。结果表明:紫薯蛋白及花色苷-蛋白复合物的分子质量无明显差异,均存在4种不同的分子质量分布:58、24、18 k D和14 k D。紫薯蛋白紫外最大吸收峰位于200 nm波长处,复合物的紫外光谱略有红移且吸收增强。紫薯蛋白荧光光谱的最大发射波长是340 nm,而复合物的荧光强度几乎完全猝灭。紫薯蛋白的红外光谱图具有酰胺带特征吸收,复合物的红外光谱峰形和吸收强度发生明显变化。圆二色谱图显示紫薯蛋白二级结构以β-折叠(43.6%)和无规卷曲(45.7%)为主,β-转角(10.6%)次之,而复合物以β-折叠(72.2%)为主,无规卷曲(27.8%)含量降低,β-转角消失。展开更多
基金Supported by The Earmarked Fund for China Agriculture Research System, No. CARS-11-B-19"Technique of Processing and Utilization for Plant Proteins" from China-Argentina Science and Technology Cooperation Program, No. 2010DFA32690+1 种基金Grant from the Capital Medical University, No. 2009ZR03 and No. 2012ZR17the Importation and Development of High-Caliber Talents Project of Beijing Municipal Institutions
文摘AIM: To investigate the effects of proteins purified from sweet potato storage roots on human colorectal cancer cell lines. METHODS: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, Hoechst 33258 nuclear staining and Boyden transwell chamber methods were used to determine whether purified sweet potato protein (SPP) from fresh sweet potato roots affected proliferation, migration and invasion, respectively, of human colorectal cancer SW480 cells in vitro . The inhibitory effects of SPP on growth of human colorectal cancer HCT-8 cells intraperitoneally xenografted in nude mice and spontaneous lung metastasis of murine Lewis lung carcinoma 3LL cells subcutaneously transplanted in C57 BL/6 mice were also investigated in vivo . RESULTS: SPP inhibited the proliferation of SW480 cells in a dose-dependent manner, with an IC 50 value of 38.732 μmol/L (r2 = 0.980, P = 0.003) in the MTT assay. Hoechst 33258 nuclear staining further revealed inhibition of cell viability and induction of apoptosis by SPP. The transwell assay disclosed significant reduction in migrated cells/field by 8 μmol/L SPP (8.4 ± 2.6 vs 23.3 ± 5.4, P = 0.031) and invaded cells/field through the ECMatrix by 0.8 μmol/L SPP, compared with the control (25.2 ± 5.2 vs 34.8 ± 6.1, P = 0.038). Both intraperitoneal (ip) and intragastric (ig) administration of SPP led to significant suppression of growth of intraperitoneally inoculated HCT-8 cells in nude mice to 58.0% ± 5.9% (P = 0.037) and 43.5% ± 7.1% (P = 0.004) of the controls, respectively, after 9 d treatment. Bloody ascites additionally disappeared after ip injection of trypsin inhibitor. Notably, ig and ip administration of SPP induced a significant decrease in spontaneous pulmonary metastatic nodule formation in C57 BL/6 mice (21.0 ± 12.3 and 27.3 ± 12.7 nodules/lung vs 42.5 ± 4.5 nodules/lung in controls, respectively, P < 0.05) after 25 d treatment. Moreover, the average weight of primary tumor nodules in the hind leg of mice decreased from 8.2 ± 1.3 g/mice in the control to 6.1 ± 1.4 g/mice in the ip group (P = 0.035). CONCLUSION: SPP exerts significant antiproliferative and antimetastatic effects on human colorectal cancer cell lines, both in vitro and in vivo .
文摘Some indices concerning the metabolism of substance and energy in sweet potato leaves under water stress were studied. The results showed an obvious increase in soluble protein content. Compared with control, Chl a, Chl b, total Chl contents and the ratio of Chl a to Chl b all decreased to some extent. ATP content increased in some varieties and decreased in others, but the stronger the drought resistance of the variety , the higher the ATP content. The correlation coefficient(r)of the soluble protein content, ratio of Chl a to Chl b and ATP content as a percentage of the drought-resistant sweet potato control variety are 0. 8968, - 0. 8509 and 0. 8200, respectively, P<0. 01. So these indices can be used to evaluate the drought resistance of different sweet potato varieties.
文摘Despite being a healthy low glycaemic index food, the production and consumption of sweet potatoes are decreasing globally. The global production trends indicated a decline from 130.47 million tonnes (MT) in 2004 to 107.64 MT in 2009, while the production in China, having the largest share towards world production of sweet potatoes, witnessed a decrease from 105.84 MT in 2004 to 81.21 MT in 2009. There is an interest in increasing consumption of healthy low glycaeimic index foods, especially in the context of a projected alarming rise in the diabetic population in the developing world by 2025. The objective of this study was to enhance the utilization of sweet potato as a low glycaemic food, mainly through its use in the development of high protein pasta. Among three protein sources, whey protein concentrate (WPC), defatted soy flour (DSF), and fish powder (FP), WPC gave high quality pasta with strong starch-protein network formation, as evidenced from scanning electron microscopic studies and low in vitro starch digestibility. Protein nutritional quality was also high for WPC-fortified sweet potato pasta, with very high scores for lysine and leucine as well as high essential amino acid index and calculated protein efficiency ratio. Fractionation of starch showed that the WPC-fortified sweet potato pasta had the lowest rapidly digested starch (RDS) and the highest resistant starch (RS) content, indicating its potential as a low glycaemic food.
文摘Sweet potato is one of the first natural GMOs, genetically modified 8000 years ago by Agrobacterium rhizogenes as reported recently by Kyndt et al. A section of 10 kbp long DNA (Transferred- DNA or T-DNA) of the Ri (Root-inducing) plasmid was transferred to the plant genome by A. rhizo-genes and has been maintained in all 291 hexaploid sweet potato cultivars of the world. The maintenance in the sweet potato genome and expression of two T-DNA genes for tryptophan-2-monooxygenease (iaaM) and for indole-3-acetamide hydrolase (iaaH) are likely to be physiologically significant since these enzymes convert tryptophan to indole-3-acetic acid, a major plant growth hormone auxin. Sweet potato (Ipomoea batatas (L.) Lam) is ranked the third most important root crop after potato and cassava, and the seventh in global food crop production with more than 126 million metric tons. Although sweet potato originated in Central or South America, China currently produces over 86% of world production with 109 million metric tons. In the United States, North Carolina is the leading producer with 38.5% of the 2007 sweet potato production, followed by California, Mississippi, and Louisiana with 23%, 19%, and 15.9%, respectively. Leaf curl virus diseases have been reported in sweet potato throughout the world. One of the causal agents is Sweet potato leaf curl virus (SPLCV) belonging to the genus Begomovirus (family Geminiviridae). Although SPLCV does not cause symptoms on Beauregard, one of the most predominant sweet potato cultivars in the US, it can reduce the yield up to 26%. Serological detection of SPLCV is not currently available due to the difficulties in obtaining purified virions that can be used as antigen for antiserum production. In attempts to obtain the coat protein (CP) of SPLCV for antibody production, primers were designed to amplify the CP gene. This gene was cloned into the expression vector pMAL-c2E as a fusion protein with maltose-binding protein, and transformed into Escherichia coli strain XL1-Blue. After gene induction, a fusion protein of 72 kDa was purified by amylose affinity chromatography. The yield of the purified fusion protein was approximately 200 μg/liter of bacterial culture. Digestion with enterokinase cleaved the fusion protein into a 42.5 kDa maltosebinding protein and a 29.4 kDa protein. The latter protein was identified by mass spectrometry analysis as the coat protein of SPLCV based on the fact that the mass spectrometry elucidated the sequences corresponding to 37% of amino acid positions of the SPLCV coat protein.
文摘为探究蛋白质和淀粉对魔芋凝胶的影响,研究不同质量分数红薯淀粉(sweet potato starch,SPS)和大豆分离蛋白(soy protein isolate,SPI)对魔芋凝胶的质地、色泽、感官、水分分布、流变学特性和微观结构的影响。单因素试验结果表明,SPS质量分数从0%增加到2.5%对魔芋凝胶质构特性有显著影响(P<0.05),硬度和弹性分别上升86.43%和27.69%,凝胶强度和持水性显著上升(P<0.05);添加2%以下的SPI显著提高凝胶的L*值和b*值(P<0.05),硬度和弹性分别上升13.41%和15.38%,凝胶强度和持水性显著上升(P<0.05),但胶黏性降低54.19%。流变学分析结果表明,随着SPS和SPI质量分数增加,凝胶G’和G”值逐渐上升,表明凝胶结构更加牢固。低场核磁共振结果表明,二者的添加显著增加了凝胶体系中结合水含量(P<0.05),自由水和弛豫时间没有显著变化。综合评分实验结果表明,优化配比后的复合凝胶质构特性进一步提高。扫描电子显微镜结果表明,优化配比后的复合凝胶形成更为致密、结构连续的稳定结构。研究表明添加2.5%SPS和1.0%SPI为复合凝胶的最佳配比。总体来说,本研究结果为魔芋凝胶在方便即食食品的应用提供良好的实验参考和理论基础。
文摘以‘紫罗兰’紫薯为原料,研究同步提取紫薯花青素以及制备紫薯淀粉、纤维素和紫薯蛋白的工艺及参数。紫薯与酸乙醇(p H 2)混合破碎、过滤、沉淀分离淀粉;将滤渣和分离淀粉后上清液混合,用微波辅助法提取花青素并优化提取条件;花青素提取液沉淀分离紫薯蛋白;提取花青素的滤渣制备紫薯纤维素。结果表明:微波辅助提取紫薯中花青素的最佳工艺条件为微波时间4 min、微波温度52℃、料液比1∶22.40(g/m L)、乙醇体积分数62%(p H 2),在此条件下紫薯花青素的提取率(93.64±0.69)%、粗提物中花青素含量(9.58±0.20)mg/g。制备的紫薯淀粉质量分数(95.77±0.41)%、得率(占总淀粉质量分数)(73.06±1.03)%;滤渣粉中纤维素含量(117.11±2.69) mg/g;制备的紫薯蛋白中蛋白质含量(524.78±24.84) mg/g。该制备方法能够提高紫薯的利用率,降低生产成本。
文摘采用硫酸铵沉淀法制备紫薯花色苷-蛋白复合物,经二乙氨基乙基纤维素52和Sephadex G-75葡聚糖凝胶纯化得紫薯纯蛋白,利用聚丙烯酰胺凝胶电泳测定紫薯蛋白及紫薯花色苷-蛋白复合物的分子质量,运用紫外、荧光、红外和圆二色谱多种光谱方法对紫薯蛋白及紫薯花色苷-蛋白复合物进行结构表征。结果表明:紫薯蛋白及花色苷-蛋白复合物的分子质量无明显差异,均存在4种不同的分子质量分布:58、24、18 k D和14 k D。紫薯蛋白紫外最大吸收峰位于200 nm波长处,复合物的紫外光谱略有红移且吸收增强。紫薯蛋白荧光光谱的最大发射波长是340 nm,而复合物的荧光强度几乎完全猝灭。紫薯蛋白的红外光谱图具有酰胺带特征吸收,复合物的红外光谱峰形和吸收强度发生明显变化。圆二色谱图显示紫薯蛋白二级结构以β-折叠(43.6%)和无规卷曲(45.7%)为主,β-转角(10.6%)次之,而复合物以β-折叠(72.2%)为主,无规卷曲(27.8%)含量降低,β-转角消失。
