[ Objective] To establish the standardized spectrophotometric method to determine boar sperm concentration. [ Method ] The relation- ships between absorbance （A）, transmittance （T） and sperm concentration （C） of...[ Objective] To establish the standardized spectrophotometric method to determine boar sperm concentration. [ Method ] The relation- ships between absorbance （A）, transmittance （T） and sperm concentration （C） of different wavelengths （450, 550, 650 nm） were compared. [ Result] The maximum sperm concentration detected by absorbance presented an upward trend with the increase of the wavelengths, 202 mitliorVml （450 nm）, 224 million/ml （550 nm） and 235 mUlion/ml （650 nm）, respectively, but the stability of repeated measurement was decreased. With the increase of sperm dilution times, the stability of repeated measurement of transmittance was reduced, and when dilution times were more than 10 times （450 nm）, 6 times （550 nm） and 4 times （650 nm）, differences appeared between the observed values of repeated measurement. [ Con- clusion] Wavelength at 450 nm was found to be the most sensitive and reliable, and sperm concentration presented cubic functional regression rela- tionship or power functional regression relationship with absorbance or transmittance, respectively. The regression equation for the standard curve at 450 nm was C400 = 0.48A3 - 0.76A2 ＋ 0.67A - 0.066 （ R = 0.951 ） and C400 = 1.657T -0.108. 8 （ R = 0.940）.展开更多
文摘[ Objective] To establish the standardized spectrophotometric method to determine boar sperm concentration. [ Method ] The relation- ships between absorbance （A）, transmittance （T） and sperm concentration （C） of different wavelengths （450, 550, 650 nm） were compared. [ Result] The maximum sperm concentration detected by absorbance presented an upward trend with the increase of the wavelengths, 202 mitliorVml （450 nm）, 224 million/ml （550 nm） and 235 mUlion/ml （650 nm）, respectively, but the stability of repeated measurement was decreased. With the increase of sperm dilution times, the stability of repeated measurement of transmittance was reduced, and when dilution times were more than 10 times （450 nm）, 6 times （550 nm） and 4 times （650 nm）, differences appeared between the observed values of repeated measurement. [ Con- clusion] Wavelength at 450 nm was found to be the most sensitive and reliable, and sperm concentration presented cubic functional regression rela- tionship or power functional regression relationship with absorbance or transmittance, respectively. The regression equation for the standard curve at 450 nm was C400 = 0.48A3 - 0.76A2 ＋ 0.67A - 0.066 （ R = 0.951 ） and C400 = 1.657T -0.108. 8 （ R = 0.940）.
