[ Objective] To investigate the blocking effects of spleen vaccine on vertical transmission of classical swine fever virus （CSFV） in sows. [ Method] Sows infected by CSFV were selected from three large-scale pig far...[ Objective] To investigate the blocking effects of spleen vaccine on vertical transmission of classical swine fever virus （CSFV） in sows. [ Method] Sows infected by CSFV were selected from three large-scale pig farms and they were randomly divided into group Ⅰ, group Ⅱ and control group. The sows in the group Ⅰ were vaccinated with CSF spleen vaccine at a 1.5 times normal dose per pig; those in the group Ⅱ were vaccinated with CSF spleen vaccine at a 2.0 times normal dose per pig; and those in the control group were vaccinated with cell vaccine at a 4.0 times normal- dose per pig. The CSF antigens of piglets were detected by enzyme-linked immunosorbent assay （ELISA）. [ Result] The antigen positive rate of piglets in the experimental group （18.5%） was significantly lower than that in the control group （48.1% ）. No significant difference was found be- tween the group Ⅰ and the group Ⅱ. [ Condmion] CSF spleen vaccine has good blocking effects on vertical transmission of CSFV in sows.展开更多
报道了从猪脾制备可提取核抗原(ENA)方法,从获得的纯净细胞核进一步提取抗原活性强的 ENA。ENA 组份分析表明主要含:总蛋白(TP)、DNA、RNA、组蛋白(HP)及非组蛋白(NHP),其与 DNA 的比值分别为:TP/DNA,2.91;RNA/DNA,011;HP/DNA,2.39;NHP/...报道了从猪脾制备可提取核抗原(ENA)方法,从获得的纯净细胞核进一步提取抗原活性强的 ENA。ENA 组份分析表明主要含:总蛋白(TP)、DNA、RNA、组蛋白(HP)及非组蛋白(NHP),其与 DNA 的比值分别为:TP/DNA,2.91;RNA/DNA,011;HP/DNA,2.39;NHP/DNA,0.68。ENA 经 SDS-PAGE 分析可见16条蛋白质带,分子量大都小于45KD。紫外吸收光谱在257nm 有最大吸收峰.ELISA 检测36例正常人抗 ENA 抗体(1/50稀释)均为阴性,SLE 患者抗 ENA 抗体滴度≥400。展开更多
基金supported by the Scientific Research Project Foundation of Jiangxi Provincial Education Department(GJJ08372)
文摘[ Objective] To investigate the blocking effects of spleen vaccine on vertical transmission of classical swine fever virus （CSFV） in sows. [ Method] Sows infected by CSFV were selected from three large-scale pig farms and they were randomly divided into group Ⅰ, group Ⅱ and control group. The sows in the group Ⅰ were vaccinated with CSF spleen vaccine at a 1.5 times normal dose per pig; those in the group Ⅱ were vaccinated with CSF spleen vaccine at a 2.0 times normal dose per pig; and those in the control group were vaccinated with cell vaccine at a 4.0 times normal- dose per pig. The CSF antigens of piglets were detected by enzyme-linked immunosorbent assay （ELISA）. [ Result] The antigen positive rate of piglets in the experimental group （18.5%） was significantly lower than that in the control group （48.1% ）. No significant difference was found be- tween the group Ⅰ and the group Ⅱ. [ Condmion] CSF spleen vaccine has good blocking effects on vertical transmission of CSFV in sows.
文摘报道了从猪脾制备可提取核抗原(ENA)方法,从获得的纯净细胞核进一步提取抗原活性强的 ENA。ENA 组份分析表明主要含:总蛋白(TP)、DNA、RNA、组蛋白(HP)及非组蛋白(NHP),其与 DNA 的比值分别为:TP/DNA,2.91;RNA/DNA,011;HP/DNA,2.39;NHP/DNA,0.68。ENA 经 SDS-PAGE 分析可见16条蛋白质带,分子量大都小于45KD。紫外吸收光谱在257nm 有最大吸收峰.ELISA 检测36例正常人抗 ENA 抗体(1/50稀释)均为阴性,SLE 患者抗 ENA 抗体滴度≥400。
