Study on Acid Tolerance in Rhizobium meliloti and Its Symbiosis System

Soil pH is a main factor that affects the nodule formation and nitrogen fixation. The largely reduced nitrogen fixation effect caused by acid soil is the ma- jor limitation of alfalfa growth in the south of China. Thus selection of anti-acid nodule for inoculating on alfalfa is of great significance in practical production. Using nine candidate rhizobia （ Rhizobium meliloti L. ） as study objects, their colony diameters and the nodule number of each strain inoculated alfalfa variety were investigated at different pH conditions. The YNCY006 strain presented the strongest resistance to acid, while YNCY007 strain presented the weakest. For nedulation, GT13R alfalfa inoculated with YNCY006 or YNCY008 strains was the best symbiosis system in the acidic soil.

Human-Machine Symbiosis:Philosophical Reflection on Virtual Human

Virtual human is the simulation of human under the synthesis of virtual reality,artificial intelligence,and other technologies.Modern virtual human technology simulates both the external characteristics and the internal emotions and personality of humans.The relationship between virtual human and human is a concrete expression of the modern symbiotic relationship between human and machine.This human-machine symbiosis can either be a fusion of the virtual human and the human or it can cause a split in the human itself.

Symbiosis Analysis on Industrial Ecological System

Industrial ecological system is a sustainable mode of modern industry development. Industrial symbiosis, a sub-field of industrial ecology, engages traditionally separate industries in a collective approach, involving exchange of materials, energy, water, and/or by-products, to enhance competitive ability and environmental performance. To construct a symbiosis analysis method, this article employs a number of parameters embodying information about materials, energy and economics as the main essential parameters in system analysis and introduces symbiosis profit and symbiotic consumption elements as the economic indicators. A modeling and simulation program is designed using the agent-based modeling approach to simulate the evolvement of a hypothetical coal-based industrial system and the change of symbiosis conditions in the process of construction is examined. The simulation program built using the Swarm library, which is a freely available multi-agent simulation package, provides a useful demonstration for the symbiosis analysis method.

Arbuscular mycorrhizal symbiosis and ecosystem processes: Prospects for future research in tropical soils

Arbuscular mycorrhizal fungi (AMF) are more widely distributed and can associate with a wide range of plant species. AMF are keystone organisms that form an interface between soils and plant roots. They are also sensitive to environmental changes. AMF are important microbial symbioses for plants under conditions of P-limitation. The AMF are crucial for the functioning of terrestrial ecosystems as they form symbiotic interactions with plants. Mycorrhizal fungi are known to influence plant diversity patterns in a variety of ecosystems globally. AMF hyphae form an extensive network in the soil. The length is a common parameter used to quantifying fungal hyphae. The mycelial network of AM fungi extends into the soil volume and greatly increases the surface area for the uptake of immobile nutrients. Also, AM symbioses improve plants tolerance to drought and enhance plants’ tolerance of or resistance to root pathogens. Also, the networks of AM hyphae play a crucial role in the formation of stable soil aggregates and in the building up of a macroporous structure of soil that allows penetration of water and air and thereby prevents erosion. The functioning of AMF symbiosis is mediated by direct and indirect effects of biotic and abiotic factors of the surrounding rhizosphere, the community, and the ecosystem. AMF have great potential in the restoration of disturbed land and low fertility soil. However, despite the importance of AMF to terrestrial ecosystems, little is known about the effects of environmental changes on AMF abundance, activity and the impact of these changes on the ecosystem services. Therefore, it is important to gain a clearer understanding of the effects of environmental changes on the AM fungal species to guide conservation and restoration efforts.

Research on the Industrial Symbiosis Supporting System of Eco-industrial Park

The establishment and development of the industrial symbiosis of eco-industrial park are affected by several factors.Based on the formed industrial symbiosis supporting system chart,this article analyzes the microcosmic supporting system and macroscopic supporting system.In the microcosmic supporting system,it elaborates five aspects including key enterprises,service intermediaries in the park,symbiotic enterprises,competitors and the public in detail.Then it describes the macroscopic supporting system from four aspects of governmental participation,technological innovation,educational promotion and cultural support.Finally,combining with the current construction status of the eco-industrial park in China,it proposes the countermeasures and suggestions to build the supporting system for the eco-industrial park and provides theoretical support for the faster and better construction of the eco-industrial park in China.

Research on Geographical Environment and Culture Symbiosis of Chishui River Basin

This paper took Chishui River Basin as the research object,and pointed out that its culture,which reflected local life and production and was featured with regional characteristics as well as abundant cultural connotation,was constituted by native natural and cultural geography,folk customs and historical feature.The relation between Chishui salt path and salt business culture under the typical closed and half-closed geographical environment was analyzed to indicate that the realignment of Chishui River had brought prosperity of Chishui River,booming of commercial towns,and development of ancient architectures and guild hall culture.The relation between rich natural resources and production and life culture of Chishui River was explained to show that Danxia landform nurtured stone culture,Chishui River resources cultivated fishing culture,special hydrogeological environment fostered liquor culture represented by Maotai,and Bamboo culture accumulated for hundreds of years.Finally,it introduced military culture and the spirit of the Long March forming based on the special location of Chishui River.It emphasized that geological environment was the important basis for human's survival and played an immeasurable role in the cultural development of a region.

Identification of Rhizobia Isolated from Nodules of Mexican Commercial Soybean Varieties

Rhizobia, crucial for nitrogen fixation in leguminous plants, play a vital role in soybean cultivation. This study, conducted in Mexico, a major soybean importer, aimed to identify bacteria from nodules of five soybean varieties in high-production regions. Multilocus sequence analysis (MLSA) was employed for enhanced species resolution. The study identified six Bradyrhizobium species: Bradyrhizobium japonicum USDA 110, Bradyrhizobium japonicum USDA 6, Bradyrhizobium elkanii USDA 76, Bradyrhizobium neotropicale, Bradyrhizobium lablabi, and Bradyrhizobium icense. Bradyrhizobium japonicum USDA 110 predominated in the soils, displaying symbiotic preference for the Huasteca 400 variety. However, phylogenetic analysis didn't reveal a clear association between strains, soil, and soybean variety. This research sheds light on the diversity of rhizobia in Mexican soybean cultivation, contributing to the understanding of symbiotic relationships in soybean production systems.

Symbiosis of Marshes and Permafrost in Da and Xiao Hinggan Mountains in Northeastern China

Recently, the degradation of permafrost and marsh environments in the Da and Xiao Hinggan Mountains has become a great concern as more human activities and pronounced climate warming were observed during the past 30 years and projected for the near future. The distr/bution patterns and development mechanisms of the permafrost and marshes have been examined both in theories and in field observations, in order to better understand the symbiosis of permafrost and marshes. The permafrost and marshes in the Da and Xiao Hinggan Mountains display discernible zonations in latitude and elevation. The marsh vegetation canopy, litter and peat soil have good thermal insulation properties for the underlying permafrost, resulting in a thermal offset of 3 ℃ to 4℃ and subsequently suppressing soil temperature. In addition, the much higher thermal conductivity of frozen and ice-rich peat in the active layer is conducive to the development or in favor of the protection of permafrost due to the semi-conductor properties of the soils overlying the permafrost. On the other hand, because permafrost is almost impervious, the osmosis of water in marsh soils can be effectively reduced, timely providing water supplies for helophytes growth or germination in spring. In the Da and Xiao Hinggan Mountains, the permafrost degradation has been accelerating due to the marked climate warming, ever increasing human activities, and the resultant eco-environmental changes. Since the permafrost and marsh environments are symbiotic and interdependent, they need to be managed or protected in a well-coordinated and integrated way.

Arbuscular mycorrhizal symbiosis facilitates apricot seedling(Prunus sibirica L.)growth and photosynthesis in northwest China

Arbuscular mycorrhizal(AM)fungi can successfully enhance photosynthesis(P_(n))and plants growth in agricultural or grassland ecosystems.However,how the symbionts affect species restoration in sunlight-intensive areas remains largely unexplored.Therefore,this study’s objective was to assess the effect of AM fungi on apricot seedling physiology,within a specific time period,in northwest China.In 2010,an experimental field was established in Shaanxi Province,northwest China.The experimental treatments included two AM fungi inoculation levels(0 or 100 g of AM fungal inoculum per seedling),three shade levels(1900,1100,and 550µmol m^(−2) s^(−1)),and three ages(1,3,and 5 years)of transplantation.We examined growth,Pn,and morphological indicators of apricot(Prunus sibirica L.)seedling performances in 2011,2013,and 2015.The colonization rate in mycorrhizal seedlings with similar amounts of shade is higher than the corresponding controls.The mycorrhizal seedling biomass is significantly higher than the corresponding non-mycorrhizal seedling biomass.Generally,P_(n),stomatal conductance(G_(s)),transpiration rate(T_(r)),and water use efficiency are also significantly higher in the mycorrhizal seedlings.Moreover,mycorrhizal seedlings with light shade(LS)have the highest Pn.WUE is increased in non-mycorrhizal seedlings because of the reduction in T_(r),while T_(r) is increased in mycorrhizal seedlings with shade.There is a significant increase in the N,P,and K fractions detected in roots compared with shoots.This means that LS had apparent benefits for mycorrhizal seedlings.Our results also indicate that AM fungi,combined with LS,exert a positive effect on apricot behavior.

Effect of urban symbiosis development in China on GHG emissions reduction

This paper analyzes current urban symbiosis development and application in China, and then conducts a statistical analysis of the emissions reduction of CO2 and CH4 in relation to recovery of iron and steel scraps, waste paper, and waste plastics from 2011 to 2014 using the greenhouse gas（GHG） emission inventory calculation method provided by the IPCC. Results indicate that the cumulative recovery of renewable resources during China＇s main urban symbiosis development in 2011-2014 was 803.275 Mt, and the amount of iron and steel scraps, waste paper, and waste plastic recovery was the largest, respectively accounting for 62.2%, 18.0%, and 8.2% of total recovery in 2014. In addition, the cumulative emissions reduction of GHGs in relation to recovery of iron and steel scraps, waste paper, and waste plastics in 2011-2014 was27.962 Mt CO2-eq, 954.695 Mt CO2-eq, and 22.502 Mt CO2-eq, respectively, thereby totaling 1005.159 Mt CO2-eq. Results show a remarkable GHG emissions reduction during 2011-2014.

Research status of soybean symbiosis nitrogen fixation

Soybean(Glycine max)is one of the most important economic legume crops with largest planting area,and is also an important oil crop,as well as food and feed material.Soybean-rhizobia symbiosis plays important roles in plant cultivation and fertilizer application.However,there are many problems in agricultural application of soybean symbiotic nitrogen fixation.In this review,we summarized three restriction factors(host specificity,low nitrogen fixation efficiency and abiotic stress)and discussed research progresses of these factors.Clarification of host specific mechanism will help to select and apply rhizobia inoculants.Both maintaining high nitrogenase activity and delaying nodule senescence can improve the efficiency of symbiotic nitrogen fixation.Abiotic stress-tolerant rhizobia can improve the abiotic stress tolerance of soybean.Breeding stress tolerant genotypes of soybean and rhizobia,obtaining correlated genes are the common strategies to improve soybean symbiotic nitrogen fixation under extreme conditions.Regulatory mechanisms of these restriction factors are still poorly understood and needs further clarification.

The full length <i>PtSRP</i>(<i>Pisolithus tinctorius</i>symbiosis related protein) fungal mRNA encodes a potential marker of ectomycorrhiza formation

The Pisolithus tinctorius symbiosis related protein expressed sequence tag (EST PtSRP) was previously identified in the first hours of the interaction between the fungus Pisolithus tinctorius and sweet chestnut Castanea sativa, and partially characterized as a fungal marker gene of ectomycorrhizal symbiosis formation. We used the 5’ rapid amplification of cDNA ends (RACE) to obtain the PtSRP mRNA 5’ region, and together with our previously reported 3’ mRNA region, the full mRNA sequence was assembled by use of bioinformatics tools and deposited to GenBank (Accession: GU733439). The full-length mRNA sequence (636 bp) revealed the locations of the 5’ and 3’ untranslated regions (UTRs) and contained the Kozak sequence (ccc aag ATG A) in the 5’ UTR. The in silico translated PtSRP open reading frame (ORF) codes for a 127 amino acid protein and contained four putative post-translational modification sites (two N-glycosylation and two phosphorylation). The protein secondary structure is postulated to be composed of one N-terminal hydrophobic transmembrane alpha helix and at least six hydrophilic beta-strands spread across the protein. Sub-cellular localization prediction suggests that the protein is involved in cellular secretory pathway, supported by the presence of a cleavage site motif close to the membrane anchor. The data presented herein indicate the role of PtSRP as a fungal membrane secreted protein involved in early stages of ectomycorrhizal formation, with application as a possible marker for nascent ectomy-corrhiza fungal development.

Symbiosis between fungi and the hybrid Cymbidium and its mycorrhizal microstructures

Tissue culture seedlings of the hybrid Cymbidium were inoculated with six different fungal strains, isolated from the roots of different wild terrestrial orchids. About three months later, the average increment of fresh weight of seedlings inoculated with strains CF1, CF3 and CF12 were respectively 130.26%, 345.65% and 153.34% while that of the control was only 88.40%. The differences between the three treatments and the control were statistically significant （α = 0.05）, highlighting the treatment with strain CF3 （α = 0.01）. In addition, the three strains were obtained by re-isolating. Pelotons, regarded as typical structures of orchid mycorrhiza, were also found in the inoculating roots under a microscope. It seems that the strains of CF1, CF3, and CF12 are associated with the hybrid Cymbidium and supplied the orchid with nutrition. It can be confirmed that the three strains are beneficial for the seedlings of this hybrid.

Symbiosis of language testing and language teaching:The journey of the 37^(th) Language Testing Research Colloquium( LTRC 2015) in Toronto,Canada

The 37th Language Testing Research Colloquium(LTRC 2015①)was held at Eaton Chelsea Hotel in Toronto Canada during March 16-20,2015.The first two days of March 16-17 were preconference workshop days with March 18-20 as the three main conference days.More than 300 participants from 27 countries and regions joined the conference.The top numbers of the

Fusion and Symbiosis: Innovation Practice of Grassland Ecological Culture in the Horizon of World Ecological Civilization

In the era of globalization,ecological crisis occurs frequently.As the foundation of the grassland nation,endogenetic effect of grassland ecological culture to the sustainable development of grassland is more and more important.The symbiosis theory came from the natural ecology,which includes the philosophical implication of sharing weal and woe with the universal life,and emphasizes the harmony,unity and symbiosis of all ecological elements.Nowadays if want to approach the challenge of grassland ecological culture,it should use the symbiosis and fusion theory to nourish innovation of grassland ecological culture,and construct concentric circle culture system based on organic unity of grassland nation,grassland ecological culture and world ecological civilization.It must bring the grassland ecological culture into the world ecological civilization,so both two should interact each other.It also should create an internal security mechanism by using the co-treatment thinking to strengthen the grassland ecological culture.All of these should basically find a way about organic fusion and sustainable development of grassland nation and global natural ecology.

Symbiosis of Mining Heritage and Community: A Case Study of Zhongfu Mining Heritage in Jiaozuo

This study selected three representative mining heritage sites of Zhongfu Company in Jiaozuo as the main research objects,introduced the "symbiosis" theory,explored the new ideas of mining heritage protection in the perspective of city,and put forward the community symbiosis strategy that closely combines the mining heritage protection and utilization with its community construction,urban environment and urban space,so as to realize the activation of function,integration of space and urban repair.The transformation design on this basis reflects three targeted symbiotic ways.It also provides implementation strategies on community level and residents’ life in the construction and transformation of mining cities,which is of universal and practical significance.

The Study of Alfalfa-Sinorhizobium meliloti Symbiosis Productivity under Different Water Conditions and the Influence of the Legume Seeds Lectin

The paper studies the effect of incubation of rhizobia with soybean seeds lectin on formation and functioning of alfalafa-Sinorhizobium meliloti symbiosis under the optimal water supply and drought conditions. It was shown that the addition of lectin to the inoculation compositions intensifies physiological processes in alfalfa plants： increases nitrogen fixing activity of symbiosis, nodule number on roots, as well as chlorophyll and carotenoid content in leaves, enhances plant growth during budding-fruiting period and reduces the negative effect of drought on alfalfa productivity.

Symbiosis of <i>Salmonella</i>and <i>Escherichia coli</i>by MY Phenomenon

Background: The symbiotic relationship between E. coli and Salmonella was demonstrated using hydrogen sulfide production named MY phenomenon, which is a feature of Salmonella. Methods: In order to confirm the hydrogen sulfide production of Salmonella by iron sulfide formation, deoxycholate-hydrogen sulfate-lactose agar medium having both a sulfur source and an iron source was used. The case where Salmonella was cultured alone was compared with the case co-cultured with E. coli. In the case of culture with Salmonella alone, the MY phenomenon has not occurred. When Salmonella was co-cultured with E. coli, Salmonella cultured near E. coli existing place showed a black color named MY phenomenon. When E. coli was cultured alone, it turned red due to the organic acid produced. However, when E. coli was cultured in the part where Salmonella. is inoculated on the surface of the medium, the MY phenomenon appeared there. Furthermore, hydrogen sulfide production was more active in Salmonella co-existing with E. coli. Conclusion: The study shows an important relation that E. coli is a promoting factor for Salmonella culture by NY phenomenon. The relation suggests that bacterial symbiosis relation exist in bacterial flora.

The Eclipsed Symbiosis of Aesthetics and Morality in The Benefactor

This essay expounds the indiscernible symbiosis that hitches formalist aesthetics to moral consciousness via an analysis of the fictionality of metafiction,the uncertainty of dream narrative,and Sontag’s sarcasm of anti-intellectual behaviors imbedded in her novel The Benefactor.The fictive nature of the novel is well manifested via the pervasive disclosure of self referential narration made by Hippolyte.The glaring discrepancies between the dreamlike life experiences expatiated on by Hippolyte in the main body of the novel and the circumstances laid plain en masse in his journals,letter,and manuscript which come out at the close of the novel are a strikingly acute revelation to remind a reader of the novel of the uncertainty of Hippolyte’s narration.The concluding section of the essay is an analysis of Sontag’s satirical version of anti-intellectualism:She arranges for the two abnormal characters—one is fond of daydreaming,and the other is temperamentally“camp”—to revert to the normal way of life and become intellectually pedestrian.The conclusion of the novel obscurely implies that the realities of the world have rendered it difficult for such a moral disburdenment as is set forth in Sontag’s aesthetic conceptualization to come into being.

Effect of Pesticides on Growth of Rhizobia and Their Host Plants During Symbiosis

Effect of various pesticides (insecticides, fungicides and herbicides) has been studied on growth and efficiency of symbiotic properties of 3 fast growing Rhizobium sp. under green house conditions. The results revealed adverse effects on plant growth and nitrogen fixing capacity as measured by dry weight and total nitrogen content of plants infected with pesticide treated Rhizobium. Of the pesticides tested, herbicides were found to be more effective on the above parameters than the insecticides and fungicides.