Objective:To Discuss the correlation between Hypersensitivity C-reactive Protein(Hs-CRP),Total Cholesterol(TC),Triglyceride(TG),negative emotion scale and TCM syndrome scores in“Double Heart Disease”patients with Qi...Objective:To Discuss the correlation between Hypersensitivity C-reactive Protein(Hs-CRP),Total Cholesterol(TC),Triglyceride(TG),negative emotion scale and TCM syndrome scores in“Double Heart Disease”patients with Qi stagnation blood stasis and heart gallbladder heat stagnation.Method:Fifty-two patients in Western Medicine Diagnosis of Double Heart Disease,in TCM syndrome identified as Qi stagnation blood stasis Heart gallbladder heat stagnation syndrome,detects it Hs-CRP,TC,TG levels by ELISA,use TCM Syndromes Scale to evaluate TCM Syndrome,use the Pittsburgh Sleep Quality Index(PSQI)and Self-rating symptom scale(SCL-90)to assess anxiety and sleep levels,analyze the correlation between TCM syndrome scores and Hs-CRP,TC,TG level,PSQI index,SCL-90 index.Result:There was a significant positive correlation between Hs-CRP,TG level and TCM Syndrome scores(P<0.05);TC level was postively correlated with TCM Syndrome scores,but there was no statistical significance(P>0.05);There was a significant positive correlation between PSQI index,SCL-90 index and TCM Syndrome scores(P<0.05).Conclusion:In“Double Heart Disease”patients with Qi stagnation blood stasis and heart gallbladder heat stagnation,there have characteristic syndrome changes in terms of inflammatory factor level,blood lipid level and negative emotion score;Which the above indexes can reflect the severity of TCM syndromes to a certain extent and provide the basis for the effective intervention treatment of TCM.展开更多
Objective:To evaluate the efficacy and safety of a Chinese medicine(CM)Modified Qufeng Runmian Powder(加减祛风润面散,MQFRMP)for the treatment of acne vulgaris with CM syndromes of dampness and blood stasis.Methods:In ...Objective:To evaluate the efficacy and safety of a Chinese medicine(CM)Modified Qufeng Runmian Powder(加减祛风润面散,MQFRMP)for the treatment of acne vulgaris with CM syndromes of dampness and blood stasis.Methods:In this multicenter,randomized,double-blind,placebo-controlled clinical trial,220 acne vulgaris patients with CM syndrome of dampness and blood stasis were included and randomly assigned using a central area group random design to receive either MQFRMP or the placebo,with 110 cases in each group.MQFRMP or a placebo at 145 g/bag were administered once daily for 4 weeks,respectively.The primary index of efficacy was the effective rate according to the acne severity score(ASS).The secondary indices of efficacy included the changes in the dermatology life quality index(DLQI)score,VISIA scores(spots,pores,brown spots,porphyrins and red areas)and skin assessment(skin p H,sebum amount and hydration)according to a SOFT skin multianalyzer.Results:(1)Follow-up:a total of 204 patients completed the follow-up,with 103 in the treatment group and 101 in the control group.(2)Effective rate:the total effective rate of the treatment group was significantly higher than the control group[83.5%(86/103)vs.31.7%(32/101),P<0.01)]with 95%confidence interval of 39.3%–66.4%.(3)DLQI:DLQI scores were significantly decreased the treatment and control groups(both P<0.01),but the treatment group was more obvious than the placebo group(P<0.01).(4)VISIA scores:the scores of spots,brown spots and red areas in the treatment group decreased compared with baseline(P<0.05).In the control group,the scores of brown spots and pores decreased compared with baseline(P<0.05).The improvement was more obvious in the treatment group than in the control group for all items(P<0.05).(5)Skin assessment:the p H and sebum score in the both groups decreased drastically compared with the baseline(all P<0.01),however,the improvement was more obvious in the treatment group than in the control group(P<0.01).The hydration amount in the two groups showed no statistically significant difference compared with the baseline(both P>0.05).(6)Safety:two cases of mild drug allergy were observed in the treatment group.Conclusion:MQFRMP was effective and safe for the treatment of acne vulgaris with syndromes of dampness and blood stasis.(No.ChiCTR1900020479).展开更多
Objective： To investigate the levels of cytokines related to T-helper （Th） 17 cells in serum and signal transducers in the psoriatic lesions of patients with psoriasis vulgaris of blood-heat syndrome （BHS） and bl...Objective： To investigate the levels of cytokines related to T-helper （Th） 17 cells in serum and signal transducers in the psoriatic lesions of patients with psoriasis vulgaris of blood-heat syndrome （BHS） and blood-stasis syndrome （BSS）. Methods： Sixty patients with psoriasis vulgaris were divided into the BHS and BSS groups according to the syndrome differentiation of Chinese medicine （CM）. Ten healthy subjects were considered as the control group. Cytokine levels of interleukin （IL）-17, IL-23 and IL-6 in serum were determined by enzyme-linked immunosorbent assay. Expression levels of signal transducer and activator of transcription 3 （STAT3）, p38-mitogen-activated protein kinase （MAPK） and STAT6 in the psoriatic lesions were determined using immunohistochemistry （IHC）, Western blot, and real-time quantitative reverse transcription polymerase chain reaction, respectively. Results： Production of IL-17, IL-23 and IL-6 in the BHS group and BSS group were significantly increased compared with those in the control group （P〈0.05）. Levels of IL-17 and IL-23 in the BHS group were higher than those in the BSS group （P〈0.05）. Compared with the control group, IHC positive expressions and protein expressions of STAT3 and p38-MAPK, and the STAT3 mRNA expressions in the BHS and BSS groups were significantly higher （P〈0.05 or P〈0.01）. The protein expression of STAT3 in the BHS group was significantly higher than that in the BSS group （P〈0.05）. Conclusions： Cytokines in serum and signal transducers in the psoriatic lesions alter with various CM syndromes of psoriasis. The results provide scientific basis for the treatment based on syndrome differentiation of CM in treating psoriasis vulgaris.展开更多
目的:从氨基酸代谢角度探讨乙型肝炎病毒相关慢加急性肝衰竭(HBV-ACLF)湿热瘀黄证和气虚瘀黄证的生物学基础。方法:采用靶向氨基酸代谢组学分析HBV-ACLF湿热瘀黄证组(20例)与气虚瘀黄证组(20例)患者的血清氨基酸代谢谱,寻找两种证型的...目的:从氨基酸代谢角度探讨乙型肝炎病毒相关慢加急性肝衰竭(HBV-ACLF)湿热瘀黄证和气虚瘀黄证的生物学基础。方法:采用靶向氨基酸代谢组学分析HBV-ACLF湿热瘀黄证组(20例)与气虚瘀黄证组(20例)患者的血清氨基酸代谢谱,寻找两种证型的氨基酸代谢差异特征,分析不同证型中氨基酸代谢与炎症细胞因子表达的相关性。结果:湿热瘀黄证患者体内异亮氨酸表达水平显著高于气虚瘀黄证[(10.36±4.47)μg/ml vs(7.76±4.08)μg/ml,P=0.016],但色氨酸则表达相反[(10.26±4.95)μg/ml vs(14.51±7.32)μg/ml,P=0.014];湿热瘀黄证患者白细胞介素(IL)-6显著高于气虚瘀黄证[10.15(3.88,24.42)pg/ml vs 7.73(3.93,18.83)pg/ml,P=0.017],而IL-2、IL-10显著低于气虚瘀黄证[4.40(2.88,8.55)pg/ml vs 6.60(2.80,15.50)pg/ml,P=0.033;7.19(4.52,15.04)pg/ml vs 8.21(2.19,14.64)pg/ml,P=0.015];多种氨基酸代谢与两种证型的炎症细胞因子表达密切相关,尤其是色氨酸代谢与支链氨基酸代谢;湿热瘀黄证中氨基酸代谢主要与促炎细胞因子IL-2、IL-6相关,按照相关系数大小依次为天冬氨酸、谷氨酸、异亮氨酸、亮氨酸、蛋氨酸、酪氨酸、丝氨酸、赖氨酸、谷氨酰胺、色氨酸、苯丙氨酸;气虚瘀黄证中氨基酸代谢与肿瘤坏死因子α(TNF-α)、IL-2、IL-6、IL-10相关,按照相关系数大小依次为天冬氨酸、甘氨酸、丙氨酸、苏氨酸、色氨酸、丝氨酸。异亮氨酸及色氨酸代谢不仅在两种证型间差异有统计学意义,且差异性关联炎症细胞因子的表达。结论:HBV-ACLF湿热瘀黄证和气虚瘀黄证患者在氨基酸代谢及炎症反应中均具有异质性,且两者之间相互关联。色氨酸以及支链氨基酸代谢可能对HBV-ACLF的免疫炎症反应具有重要调节作用,与湿热瘀黄证和气虚瘀黄证的生物学基础密切相关。展开更多
基金Beijing University of Traditional Chinese Medicine Fundamental Research Fund Project(No.2019-JYB-JS-174)Beijing Municipal Science and Technology Commission"Capital Characteristic Clinical Application Research"Project(No.Z161100000516136)。
文摘Objective:To Discuss the correlation between Hypersensitivity C-reactive Protein(Hs-CRP),Total Cholesterol(TC),Triglyceride(TG),negative emotion scale and TCM syndrome scores in“Double Heart Disease”patients with Qi stagnation blood stasis and heart gallbladder heat stagnation.Method:Fifty-two patients in Western Medicine Diagnosis of Double Heart Disease,in TCM syndrome identified as Qi stagnation blood stasis Heart gallbladder heat stagnation syndrome,detects it Hs-CRP,TC,TG levels by ELISA,use TCM Syndromes Scale to evaluate TCM Syndrome,use the Pittsburgh Sleep Quality Index(PSQI)and Self-rating symptom scale(SCL-90)to assess anxiety and sleep levels,analyze the correlation between TCM syndrome scores and Hs-CRP,TC,TG level,PSQI index,SCL-90 index.Result:There was a significant positive correlation between Hs-CRP,TG level and TCM Syndrome scores(P<0.05);TC level was postively correlated with TCM Syndrome scores,but there was no statistical significance(P>0.05);There was a significant positive correlation between PSQI index,SCL-90 index and TCM Syndrome scores(P<0.05).Conclusion:In“Double Heart Disease”patients with Qi stagnation blood stasis and heart gallbladder heat stagnation,there have characteristic syndrome changes in terms of inflammatory factor level,blood lipid level and negative emotion score;Which the above indexes can reflect the severity of TCM syndromes to a certain extent and provide the basis for the effective intervention treatment of TCM.
文摘Objective:To evaluate the efficacy and safety of a Chinese medicine(CM)Modified Qufeng Runmian Powder(加减祛风润面散,MQFRMP)for the treatment of acne vulgaris with CM syndromes of dampness and blood stasis.Methods:In this multicenter,randomized,double-blind,placebo-controlled clinical trial,220 acne vulgaris patients with CM syndrome of dampness and blood stasis were included and randomly assigned using a central area group random design to receive either MQFRMP or the placebo,with 110 cases in each group.MQFRMP or a placebo at 145 g/bag were administered once daily for 4 weeks,respectively.The primary index of efficacy was the effective rate according to the acne severity score(ASS).The secondary indices of efficacy included the changes in the dermatology life quality index(DLQI)score,VISIA scores(spots,pores,brown spots,porphyrins and red areas)and skin assessment(skin p H,sebum amount and hydration)according to a SOFT skin multianalyzer.Results:(1)Follow-up:a total of 204 patients completed the follow-up,with 103 in the treatment group and 101 in the control group.(2)Effective rate:the total effective rate of the treatment group was significantly higher than the control group[83.5%(86/103)vs.31.7%(32/101),P<0.01)]with 95%confidence interval of 39.3%–66.4%.(3)DLQI:DLQI scores were significantly decreased the treatment and control groups(both P<0.01),but the treatment group was more obvious than the placebo group(P<0.01).(4)VISIA scores:the scores of spots,brown spots and red areas in the treatment group decreased compared with baseline(P<0.05).In the control group,the scores of brown spots and pores decreased compared with baseline(P<0.05).The improvement was more obvious in the treatment group than in the control group for all items(P<0.05).(5)Skin assessment:the p H and sebum score in the both groups decreased drastically compared with the baseline(all P<0.01),however,the improvement was more obvious in the treatment group than in the control group(P<0.01).The hydration amount in the two groups showed no statistically significant difference compared with the baseline(both P>0.05).(6)Safety:two cases of mild drug allergy were observed in the treatment group.Conclusion:MQFRMP was effective and safe for the treatment of acne vulgaris with syndromes of dampness and blood stasis.(No.ChiCTR1900020479).
基金Supported by National Natural Science Foundation of China(No.81302971,81273764)Innovative Research Team in University of Shanghai Municipal Education Commission,China(PhaseⅡ)
文摘Objective： To investigate the levels of cytokines related to T-helper （Th） 17 cells in serum and signal transducers in the psoriatic lesions of patients with psoriasis vulgaris of blood-heat syndrome （BHS） and blood-stasis syndrome （BSS）. Methods： Sixty patients with psoriasis vulgaris were divided into the BHS and BSS groups according to the syndrome differentiation of Chinese medicine （CM）. Ten healthy subjects were considered as the control group. Cytokine levels of interleukin （IL）-17, IL-23 and IL-6 in serum were determined by enzyme-linked immunosorbent assay. Expression levels of signal transducer and activator of transcription 3 （STAT3）, p38-mitogen-activated protein kinase （MAPK） and STAT6 in the psoriatic lesions were determined using immunohistochemistry （IHC）, Western blot, and real-time quantitative reverse transcription polymerase chain reaction, respectively. Results： Production of IL-17, IL-23 and IL-6 in the BHS group and BSS group were significantly increased compared with those in the control group （P〈0.05）. Levels of IL-17 and IL-23 in the BHS group were higher than those in the BSS group （P〈0.05）. Compared with the control group, IHC positive expressions and protein expressions of STAT3 and p38-MAPK, and the STAT3 mRNA expressions in the BHS and BSS groups were significantly higher （P〈0.05 or P〈0.01）. The protein expression of STAT3 in the BHS group was significantly higher than that in the BSS group （P〈0.05）. Conclusions： Cytokines in serum and signal transducers in the psoriatic lesions alter with various CM syndromes of psoriasis. The results provide scientific basis for the treatment based on syndrome differentiation of CM in treating psoriasis vulgaris.
文摘目的:从氨基酸代谢角度探讨乙型肝炎病毒相关慢加急性肝衰竭(HBV-ACLF)湿热瘀黄证和气虚瘀黄证的生物学基础。方法:采用靶向氨基酸代谢组学分析HBV-ACLF湿热瘀黄证组(20例)与气虚瘀黄证组(20例)患者的血清氨基酸代谢谱,寻找两种证型的氨基酸代谢差异特征,分析不同证型中氨基酸代谢与炎症细胞因子表达的相关性。结果:湿热瘀黄证患者体内异亮氨酸表达水平显著高于气虚瘀黄证[(10.36±4.47)μg/ml vs(7.76±4.08)μg/ml,P=0.016],但色氨酸则表达相反[(10.26±4.95)μg/ml vs(14.51±7.32)μg/ml,P=0.014];湿热瘀黄证患者白细胞介素(IL)-6显著高于气虚瘀黄证[10.15(3.88,24.42)pg/ml vs 7.73(3.93,18.83)pg/ml,P=0.017],而IL-2、IL-10显著低于气虚瘀黄证[4.40(2.88,8.55)pg/ml vs 6.60(2.80,15.50)pg/ml,P=0.033;7.19(4.52,15.04)pg/ml vs 8.21(2.19,14.64)pg/ml,P=0.015];多种氨基酸代谢与两种证型的炎症细胞因子表达密切相关,尤其是色氨酸代谢与支链氨基酸代谢;湿热瘀黄证中氨基酸代谢主要与促炎细胞因子IL-2、IL-6相关,按照相关系数大小依次为天冬氨酸、谷氨酸、异亮氨酸、亮氨酸、蛋氨酸、酪氨酸、丝氨酸、赖氨酸、谷氨酰胺、色氨酸、苯丙氨酸;气虚瘀黄证中氨基酸代谢与肿瘤坏死因子α(TNF-α)、IL-2、IL-6、IL-10相关,按照相关系数大小依次为天冬氨酸、甘氨酸、丙氨酸、苏氨酸、色氨酸、丝氨酸。异亮氨酸及色氨酸代谢不仅在两种证型间差异有统计学意义,且差异性关联炎症细胞因子的表达。结论:HBV-ACLF湿热瘀黄证和气虚瘀黄证患者在氨基酸代谢及炎症反应中均具有异质性,且两者之间相互关联。色氨酸以及支链氨基酸代谢可能对HBV-ACLF的免疫炎症反应具有重要调节作用,与湿热瘀黄证和气虚瘀黄证的生物学基础密切相关。
