The technique of ferrography has been applied to study the wear particles in synovial fluid of human knee joints. As a result some discrete, identifiable kinds of wear particles were found and various wear mechanisms,...The technique of ferrography has been applied to study the wear particles in synovial fluid of human knee joints. As a result some discrete, identifiable kinds of wear particles were found and various wear mechanisms, for example, adhesive wear, fatigue wear,etc. were revealed. Ferrographic techique may provide a method for early differential diagnosis, and prognostication concerning the future course of the disease.展开更多
Objective: Analysis of the effect of nanobacteria on the expression of local biomarkers in patients with rheumatoid arthritis. Methods: Serum samples from 54 patients with rheumatoid arthritis in our hospital were tes...Objective: Analysis of the effect of nanobacteria on the expression of local biomarkers in patients with rheumatoid arthritis. Methods: Serum samples from 54 patients with rheumatoid arthritis in our hospital were tested for nanobacteria, and were divided into three groups based on the concentration of nanobacteria. To compare the differences of main essential elements, serum cytokines, immunochemical indicators and bone metabolic markers in synovial fluid of three groups of patients. Results: Differences in serum calcium levels between the three groups are significant. The differences in IL-1, TNF-α and CRP were significant. The difference of IgM, IgA, anti-CCPP content are significant. CTX-1, RF, ESR content differences are significant. Conclusions: The detection of nanobacteria in joint fluid of patients with rheumatoid arthritis has certain effects on the expression of serum cytokines and immune indexes. The higher the concentration of nanobacteria, the higher the expression of inflammatory and immunological indicators in serum.展开更多
Purpose:There were 10%e30%of patients with adult-onset septic arthritis(SA)exhibiting sterile synovial fluid(SF),and the uncertainty in the determining diagnosis of these patients posed a challenge in management.The p...Purpose:There were 10%e30%of patients with adult-onset septic arthritis(SA)exhibiting sterile synovial fluid(SF),and the uncertainty in the determining diagnosis of these patients posed a challenge in management.The purpose of this study was to investigate the differences between confirmed(Newman A)and suspected(Newman B&C)SA in adults.Methods:This was a descriptive study with a cross-sectional study design conducted at a tertiary referral centre from July 2016 to February 2019.Patients aged over 18 years presented to the emergency department with clinical features suggestive of SA and were scheduled to undergo arthrotomy and joint lavage by the treating surgeon were included in the study.Patients with prosthetic joint infections and open joint injuries were excluded.Patients’demographic data,clinical features and laboratory parameters were collected.The clinical and laboratory profile(blood and SF)of the adult patients presenting with features suggestive of SA based on Newman criteria was statistically analyzed by SPSS version 20 software and Microsoft Excel.The categorical variables were expressed as proportions while the continuous variables were expressed as mean(SD)or median(IQR)depending upon the normality of distribution.The difference between the two groups for categorical variables was assessed using the Chisquare test and the difference for continuous variables was assessed using the unpaired t-test and the Mann-Whitney test depending upon normality.A p value<0.05 was considered significant.Results:Thirty-six patients were divided into confirmed(n¼19)or suspected(n¼17)SA for assessment based on SF culture.The median(IQR)age of the patients was 50 years(37e60 years).There was no significant difference in demographic,clinical and laboratory parameters between the concerned groups.Eight patients presented with fever.Among the confirmed SA cases,8 were negative for C-reactive protein and 6 had synovial white blood cell count<50,000.Staphylococcus species were isolated in 8 cases.The most common risk factors for SA were chronic kidney disease(25.0%),diabetes mellitus(25.0%),pharmacologic immunosuppression(16.7%),recent joint surgery(11.1%)and distant site infection(11.1%).Conclusion:SA is an orthopaedic emergency that needs prompt and aggressive treatment to prevent catastrophic complications.Confirmed and suspected cases of SA exhibit similar demography,clinical features and laboratory parameters at presentation which may mislead the treating surgeon.Management should be based on sound clinical judgment in the event of failure to culture microorganisms.展开更多
Unlike bone marrow(BM)mesenchymal stem cells(MSCs),whose in vivo identity has been actively explored in recent years,the biology of MSCs in the synovium remains poorly understood.Synovial MSCs may be of great importan...Unlike bone marrow(BM)mesenchymal stem cells(MSCs),whose in vivo identity has been actively explored in recent years,the biology of MSCs in the synovium remains poorly understood.Synovial MSCs may be of great importance to rheumatology and orthopedics because of the direct proximity and accessibility of the synovium to cartilage,ligament,and meniscus.Their excellent chondrogenic capabilities and suggested transit through the synovial fluid,giving unhindered access to the joint surface,further support a pivotal role for synovial MSCs in homeostatic joint repair.This review highlights several unresolved issues pertaining to synovial MSC isolation,topography,and their relationship with pericytes,synovial fibroblasts,and synovial fluid MSCs.Critically reviewing published data on synovial MSCs,we also draw from our experience of exploring the in vivo biology of MSCs in the BM to highlight key differences.Extending our knowledge of synovial MSCs in vivo could lead to novel therapeutic strategies for arthritic diseases.展开更多
目的研究Foxp3+调节性T细胞(regulatory T cell,Treg)在类风湿关节炎患者外周血和关节液的含量及其与临床的相关性。方法采集91例RA患者和20例健康人外周血,常规关节穿刺术抽取RA患者关节液,采用流式细胞术检测Foxp3+Treg细胞百分含量,...目的研究Foxp3+调节性T细胞(regulatory T cell,Treg)在类风湿关节炎患者外周血和关节液的含量及其与临床的相关性。方法采集91例RA患者和20例健康人外周血,常规关节穿刺术抽取RA患者关节液,采用流式细胞术检测Foxp3+Treg细胞百分含量,并研究其与RA患者病情活动程度、血沉(ESR)、C反应蛋白(CRP)、类风湿因子(RF)的关系。结果 RA组患者外周血Foxp3+Treg细胞的百分含量低于健康对照组(P<0.05),而与DAS28评分分组、ESR、CRP、RF无相关性(P>0.05);RA组患者关节液Foxp3+Treg细胞的百分含量高于自身外周血(P<0.01),且与DAS28评分分组、ESR、CRP有负相关性(P均<0.01);RA组患者糖皮质激素治疗3个月后外周血和关节液中Foxp3+Treg细胞百分率较治疗前高(P均<0.05)。结论Foxp3+Treg细胞在RA发病机制上起重要作用。外周血Foxp3+Treg细胞降低导致其负调控作用的减弱,促进了疾病的发生和发展,聚集于关节液中Foxp3+T细胞,不能抑制疾病的发生,但在控制炎症程度发挥了一定作用。监测患者外周血Foxp3+Treg细胞水平可作为糖皮质激素治疗的疗效判断。展开更多
文摘The technique of ferrography has been applied to study the wear particles in synovial fluid of human knee joints. As a result some discrete, identifiable kinds of wear particles were found and various wear mechanisms, for example, adhesive wear, fatigue wear,etc. were revealed. Ferrographic techique may provide a method for early differential diagnosis, and prognostication concerning the future course of the disease.
文摘Objective: Analysis of the effect of nanobacteria on the expression of local biomarkers in patients with rheumatoid arthritis. Methods: Serum samples from 54 patients with rheumatoid arthritis in our hospital were tested for nanobacteria, and were divided into three groups based on the concentration of nanobacteria. To compare the differences of main essential elements, serum cytokines, immunochemical indicators and bone metabolic markers in synovial fluid of three groups of patients. Results: Differences in serum calcium levels between the three groups are significant. The differences in IL-1, TNF-α and CRP were significant. The difference of IgM, IgA, anti-CCPP content are significant. CTX-1, RF, ESR content differences are significant. Conclusions: The detection of nanobacteria in joint fluid of patients with rheumatoid arthritis has certain effects on the expression of serum cytokines and immune indexes. The higher the concentration of nanobacteria, the higher the expression of inflammatory and immunological indicators in serum.
文摘Purpose:There were 10%e30%of patients with adult-onset septic arthritis(SA)exhibiting sterile synovial fluid(SF),and the uncertainty in the determining diagnosis of these patients posed a challenge in management.The purpose of this study was to investigate the differences between confirmed(Newman A)and suspected(Newman B&C)SA in adults.Methods:This was a descriptive study with a cross-sectional study design conducted at a tertiary referral centre from July 2016 to February 2019.Patients aged over 18 years presented to the emergency department with clinical features suggestive of SA and were scheduled to undergo arthrotomy and joint lavage by the treating surgeon were included in the study.Patients with prosthetic joint infections and open joint injuries were excluded.Patients’demographic data,clinical features and laboratory parameters were collected.The clinical and laboratory profile(blood and SF)of the adult patients presenting with features suggestive of SA based on Newman criteria was statistically analyzed by SPSS version 20 software and Microsoft Excel.The categorical variables were expressed as proportions while the continuous variables were expressed as mean(SD)or median(IQR)depending upon the normality of distribution.The difference between the two groups for categorical variables was assessed using the Chisquare test and the difference for continuous variables was assessed using the unpaired t-test and the Mann-Whitney test depending upon normality.A p value<0.05 was considered significant.Results:Thirty-six patients were divided into confirmed(n¼19)or suspected(n¼17)SA for assessment based on SF culture.The median(IQR)age of the patients was 50 years(37e60 years).There was no significant difference in demographic,clinical and laboratory parameters between the concerned groups.Eight patients presented with fever.Among the confirmed SA cases,8 were negative for C-reactive protein and 6 had synovial white blood cell count<50,000.Staphylococcus species were isolated in 8 cases.The most common risk factors for SA were chronic kidney disease(25.0%),diabetes mellitus(25.0%),pharmacologic immunosuppression(16.7%),recent joint surgery(11.1%)and distant site infection(11.1%).Conclusion:SA is an orthopaedic emergency that needs prompt and aggressive treatment to prevent catastrophic complications.Confirmed and suspected cases of SA exhibit similar demography,clinical features and laboratory parameters at presentation which may mislead the treating surgeon.Management should be based on sound clinical judgment in the event of failure to culture microorganisms.
文摘Unlike bone marrow(BM)mesenchymal stem cells(MSCs),whose in vivo identity has been actively explored in recent years,the biology of MSCs in the synovium remains poorly understood.Synovial MSCs may be of great importance to rheumatology and orthopedics because of the direct proximity and accessibility of the synovium to cartilage,ligament,and meniscus.Their excellent chondrogenic capabilities and suggested transit through the synovial fluid,giving unhindered access to the joint surface,further support a pivotal role for synovial MSCs in homeostatic joint repair.This review highlights several unresolved issues pertaining to synovial MSC isolation,topography,and their relationship with pericytes,synovial fibroblasts,and synovial fluid MSCs.Critically reviewing published data on synovial MSCs,we also draw from our experience of exploring the in vivo biology of MSCs in the BM to highlight key differences.Extending our knowledge of synovial MSCs in vivo could lead to novel therapeutic strategies for arthritic diseases.
文摘目的研究Foxp3+调节性T细胞(regulatory T cell,Treg)在类风湿关节炎患者外周血和关节液的含量及其与临床的相关性。方法采集91例RA患者和20例健康人外周血,常规关节穿刺术抽取RA患者关节液,采用流式细胞术检测Foxp3+Treg细胞百分含量,并研究其与RA患者病情活动程度、血沉(ESR)、C反应蛋白(CRP)、类风湿因子(RF)的关系。结果 RA组患者外周血Foxp3+Treg细胞的百分含量低于健康对照组(P<0.05),而与DAS28评分分组、ESR、CRP、RF无相关性(P>0.05);RA组患者关节液Foxp3+Treg细胞的百分含量高于自身外周血(P<0.01),且与DAS28评分分组、ESR、CRP有负相关性(P均<0.01);RA组患者糖皮质激素治疗3个月后外周血和关节液中Foxp3+Treg细胞百分率较治疗前高(P均<0.05)。结论Foxp3+Treg细胞在RA发病机制上起重要作用。外周血Foxp3+Treg细胞降低导致其负调控作用的减弱,促进了疾病的发生和发展,聚集于关节液中Foxp3+T细胞,不能抑制疾病的发生,但在控制炎症程度发挥了一定作用。监测患者外周血Foxp3+Treg细胞水平可作为糖皮质激素治疗的疗效判断。
