Influence of sodium fluoride on alkaline phosphatase activity and bone gla protein synthesis in human yellow ligament cells in vitro

Objective To investigate the influence of sodium fluoride(NaF)on alkaline phosphatase(ALP)activity and bone gla protein(BGP)synthesis in yellow ligament cells from different surgical simples in vitro.Methods The human ligament cells

Emerging tropane alkaloids:Global development and potential health threats

Tropane alkaloids(TAs)are a kind of plant secondary metabolite that mainly originate from Solanaceae.They have potent anticholinergic activity,and are well-known anticholinergic drugs,but have also been reported as plant toxins.Many studies have been conducted on TAs,but no scientometric research has been performed.This study aimed to expound the knowledge network and development of the feld of TAs and predict the emerging development topics based on bibliometrics.In particular,this research combined VOSviewer and CiteSpace for visualization and covered 1,298 related scientifc publications(1953–2022).The research on TAs is rapidly developing,with the participation of 86 countries,1,129 research institutions,and 4,087 researchers.China,the University of Geneva,and P.Christen are the most productive country,institution,and researcher,respectively.Currently,the main research topics on TAs include biosynthesis,in vitro synthesis,chemical synthesis,and determination methods.Since 2018,the monitoring of TAs in food related to human health has shown citation burst characteristics,which is considered an emerging development trend in the future.The fndings of this study will enable researchers to quickly tap into the knowledge background/structure of TAs from massive data to provide a reference for further research.

Synthesis and structure-activity relationships study ofα-aminophosphonate derivatives containing a quinoline moiety

Two series of a-aminophosphonate derivatives containing a quinoline moiety have been designed and synthesized by introducing bioactive quinoline scaffold to a-aminophosphonate. The in vitro cytotoxic activities of target compounds were first investigated against two human cancer cell lines including Eca109 and Huh7 by MTT assay. Results revealed that most of target compounds exhibited moderate to high antitumor activities against the tested cancer cell lines and some demonstrated more potent inhibitory activities compared with Sunitinib. Among them, compounds 4b2 and 4b4 containing methylsubstituted aniline group were found to be more active than Sunitinib against both of two cancer cell lines, with IC50 in the range of 2.26 mmol/L–7.46 mmol/L.

Methods to reduce variability in E. Coli-based cell-free protein expression experiments

Cell-free protein synthesis(CFPS)is an established biotechnology tool that has shown great utility in many applications such as prototyping proteins,building genetic circuits,designing biosensors,and expressing cytotoxic proteins.Although CFPS has been widely deployed,the many,varied methods presented in the literature can be challenging for new users to adopt.From our experience and others who newly enter the field,one of the most frustrating aspects of applying CFPS as a laboratory can be the large levels of variability that are present within experimental replicates.Herein we provide a retrospective summary of CFPS methods that reduce variability significantly.These methods include optimized extract preparation,fully solubilizing the master mix components,and careful mixing of the reaction.These have reduced our coefficient of variation from 97.3%to 1.2%.Moreover,these methods allow complete novices(e.g.semester rotation undergraduate students)to provide data that is comparable to experienced users,thus allowing broader participation in this exciting research area.