In the middle of the 7th Century,having sMar-yig as a blueprint and referring to the ancient Indian Pada,Thonmi Sambhota worked out Tibetan script,and confirmed the two major calligraphies:Urgyen(regular)and Urme(runn...In the middle of the 7th Century,having sMar-yig as a blueprint and referring to the ancient Indian Pada,Thonmi Sambhota worked out Tibetan script,and confirmed the two major calligraphies:Urgyen(regular)and Urme(running hand).According to the historical literature,eight different calligraphies have turned up in the展开更多
[Objective] The paper aimed to construct a new T vector based on plasmid pUC19 digested with Xcm Ⅰ. [Method] Two complementary oligonucleotide chains containing two Xcm Ⅰ sites were synthesized. After denaturation a...[Objective] The paper aimed to construct a new T vector based on plasmid pUC19 digested with Xcm Ⅰ. [Method] Two complementary oligonucleotide chains containing two Xcm Ⅰ sites were synthesized. After denaturation and renaturation,the adaptor was cloned into plasmid pUC19 between the Hind Ⅲ and BamH Ⅰ sites. The new plasmid,pUC19-HB-T vector,was digested with Xcm Ⅰ to derive a T-vector with 3′ end overhanging a T base. [Result] The constructed pUC19-HB-T vector was efficient in cloning PCR products,with an efficiency of 95% at least. [Conclusion] A new Xcm Ⅰ-based pUC19-HB-T vector was constructed,which could be applied to cloning of PCR products and other microbiology operations.展开更多
文摘In the middle of the 7th Century,having sMar-yig as a blueprint and referring to the ancient Indian Pada,Thonmi Sambhota worked out Tibetan script,and confirmed the two major calligraphies:Urgyen(regular)and Urme(running hand).According to the historical literature,eight different calligraphies have turned up in the
文摘[Objective] The paper aimed to construct a new T vector based on plasmid pUC19 digested with Xcm Ⅰ. [Method] Two complementary oligonucleotide chains containing two Xcm Ⅰ sites were synthesized. After denaturation and renaturation,the adaptor was cloned into plasmid pUC19 between the Hind Ⅲ and BamH Ⅰ sites. The new plasmid,pUC19-HB-T vector,was digested with Xcm Ⅰ to derive a T-vector with 3′ end overhanging a T base. [Result] The constructed pUC19-HB-T vector was efficient in cloning PCR products,with an efficiency of 95% at least. [Conclusion] A new Xcm Ⅰ-based pUC19-HB-T vector was constructed,which could be applied to cloning of PCR products and other microbiology operations.
