[Objective] The research aimed at optimizing protein removal method and condition of polysaccharide extracts from Phellinus Linteus and comparing the effects of two methods on protein removal.[Method] Free proteins in...[Objective] The research aimed at optimizing protein removal method and condition of polysaccharide extracts from Phellinus Linteus and comparing the effects of two methods on protein removal.[Method] Free proteins in polysaccharide from Phellinus Linteus were removed using Sevag method and TCA method.[Result] The TCA method was better than Sevag method,and the optimum protein removal condition was treated with 5% TCA for 30 min and for three times,under that condition,the protein removal rate attained 82% while the polysaccharide loss rate was only 10.8%.[Conclusion] The TCA method was a better way to remove proteins of polysaccharide from Phellinus Linteus.展开更多
AIM: To assess the adhesion- and abscess-reducing capacities of various concentrations of polysaccharides derived from fungus, Phellinus gilvus (PG) or Phellinus linteus (PL) in a rat peritonitis model.METHODS: In 96 ...AIM: To assess the adhesion- and abscess-reducing capacities of various concentrations of polysaccharides derived from fungus, Phellinus gilvus (PG) or Phellinus linteus (PL) in a rat peritonitis model.METHODS: In 96 SD rats, experimental peritonitis was induced using the cecal ligation and puncture model (CLP).Rats were randomly assigned to 8 groups; Ringer's lactate solution (RL group), hyaluronic acid (HA group), 0.025%,0.25%, and 0.5% polysaccharides from PG (PG0.025, 0.25,and 0.5 groups), and PL (PL0.025, 0.25, and 0.5 groups).Adhesions and abscesses were noted at 7 d after CLP.RT-PCR assay was performed to assess the cecal tissue.RESULTS: Adhesion formation was significantly reduced in PG0.25, 0.5, PL0.25, 0.5, and HA groups (2.5±0.7,2.4±0.7, 3.8±1.0, 3.6±0.8, and 2.7±1.1, P＜0.05). The incidence of abscesses was significantly reduced in all treated groups compared to RL group (58%, P＜0.05). The urokinase-type plasminogen activator (uPA) gene expression was greatly up-regulated by increasing the concentration of polysaccharides. The urokinase-type plasminogen activator receptor (uPAR) and tumor necrosis factor (TNF)-α mRNA were highly expressed in PG0.25, 0.5, PL0.25, and 0.5groups.CONCLUSION: We concluded that 0.5% polysaccharide derived from PG and PL was the optimal concentration in preventing adhesion and abscess formation and may act by modulating activity of uPA and TNF-α in a rat peritonitis model.展开更多
对桑黄固体和液体发酵培养条件及菌丝多糖的提取工艺进行优化。结果表明:桑黄液体发酵培养基的最佳碳源是玉米粉和黄豆粉,最佳氮源是酵母浸出汁和黄豆粉,最佳碳氮比25∶1,温度28℃,装液量80 m L,转速110 r/min,发酵培养时间9天。以巨菌...对桑黄固体和液体发酵培养条件及菌丝多糖的提取工艺进行优化。结果表明:桑黄液体发酵培养基的最佳碳源是玉米粉和黄豆粉,最佳氮源是酵母浸出汁和黄豆粉,最佳碳氮比25∶1,温度28℃,装液量80 m L,转速110 r/min,发酵培养时间9天。以巨菌草为主要基料进行固体发酵培养,桑黄菌丝长势良好。巨菌草发酵培养的桑黄菌丝多糖的最佳提取工艺为温度90℃,料液比1∶40,提取时间1.5 h,以3倍体积的乙醇醇沉多糖的条件下多糖得率最高,达到12.06%,高于其他栽培方式的桑黄菌丝体多糖含量。展开更多
基金Supported by Youth Fund Project in Sichuan Province(2008ZQ026-072)Support Science and Technology Project of Sichuan Province(2008FZ0157)~~
文摘[Objective] The research aimed at optimizing protein removal method and condition of polysaccharide extracts from Phellinus Linteus and comparing the effects of two methods on protein removal.[Method] Free proteins in polysaccharide from Phellinus Linteus were removed using Sevag method and TCA method.[Result] The TCA method was better than Sevag method,and the optimum protein removal condition was treated with 5% TCA for 30 min and for three times,under that condition,the protein removal rate attained 82% while the polysaccharide loss rate was only 10.8%.[Conclusion] The TCA method was a better way to remove proteins of polysaccharide from Phellinus Linteus.
基金Supported by Grant R08-2003-000-10120-0 from the Basic Research Program of the Korea Science and Engineering Foundation.
文摘AIM: To assess the adhesion- and abscess-reducing capacities of various concentrations of polysaccharides derived from fungus, Phellinus gilvus (PG) or Phellinus linteus (PL) in a rat peritonitis model.METHODS: In 96 SD rats, experimental peritonitis was induced using the cecal ligation and puncture model (CLP).Rats were randomly assigned to 8 groups; Ringer's lactate solution (RL group), hyaluronic acid (HA group), 0.025%,0.25%, and 0.5% polysaccharides from PG (PG0.025, 0.25,and 0.5 groups), and PL (PL0.025, 0.25, and 0.5 groups).Adhesions and abscesses were noted at 7 d after CLP.RT-PCR assay was performed to assess the cecal tissue.RESULTS: Adhesion formation was significantly reduced in PG0.25, 0.5, PL0.25, 0.5, and HA groups (2.5±0.7,2.4±0.7, 3.8±1.0, 3.6±0.8, and 2.7±1.1, P＜0.05). The incidence of abscesses was significantly reduced in all treated groups compared to RL group (58%, P＜0.05). The urokinase-type plasminogen activator (uPA) gene expression was greatly up-regulated by increasing the concentration of polysaccharides. The urokinase-type plasminogen activator receptor (uPAR) and tumor necrosis factor (TNF)-α mRNA were highly expressed in PG0.25, 0.5, PL0.25, and 0.5groups.CONCLUSION: We concluded that 0.5% polysaccharide derived from PG and PL was the optimal concentration in preventing adhesion and abscess formation and may act by modulating activity of uPA and TNF-α in a rat peritonitis model.
文摘对桑黄固体和液体发酵培养条件及菌丝多糖的提取工艺进行优化。结果表明:桑黄液体发酵培养基的最佳碳源是玉米粉和黄豆粉,最佳氮源是酵母浸出汁和黄豆粉,最佳碳氮比25∶1,温度28℃,装液量80 m L,转速110 r/min,发酵培养时间9天。以巨菌草为主要基料进行固体发酵培养,桑黄菌丝长势良好。巨菌草发酵培养的桑黄菌丝多糖的最佳提取工艺为温度90℃,料液比1∶40,提取时间1.5 h,以3倍体积的乙醇醇沉多糖的条件下多糖得率最高,达到12.06%,高于其他栽培方式的桑黄菌丝体多糖含量。
