Wildfire disease (caused by Pseudomonas syringae pv. tabaci) is one of the major diseases of tobacco, which occurs at a frequency of 40％—50％ and sometimes even more than 90％ in tobacco growing areas such as Yunnan...Wildfire disease (caused by Pseudomonas syringae pv. tabaci) is one of the major diseases of tobacco, which occurs at a frequency of 40％—50％ and sometimes even more than 90％ in tobacco growing areas such as Yunnan Province every year. As the disease spots caused by infection will continue to expand obviously while being dried after harvest, the loss in tobacco yield and quality is far greater. It seems展开更多
Using genetic recombinant techniques, the tabtoxin resistant gene (trc gene) was to ligated a pBI121 vector under the CaMV 35S promoter to form the binary vector pBITR. Then the vector was introduced into Agrobacteriu...Using genetic recombinant techniques, the tabtoxin resistant gene (trc gene) was to ligated a pBI121 vector under the CaMV 35S promoter to form the binary vector pBITR. Then the vector was introduced into Agrobacterium tumefaciens by triparental mating. After transforming the tobacco, many transgenic plants were obtained. PCR tests indicated that the trc gene was inserted in the genome DNA of tobacco. Biological activity analysis also demonstrated that the transgenic plant alleviated the symptom caused by the pathogen of tobacco wildfire disease.展开更多
基金Project supported by the National Natural Science Foundation of China.
文摘Wildfire disease (caused by Pseudomonas syringae pv. tabaci) is one of the major diseases of tobacco, which occurs at a frequency of 40％—50％ and sometimes even more than 90％ in tobacco growing areas such as Yunnan Province every year. As the disease spots caused by infection will continue to expand obviously while being dried after harvest, the loss in tobacco yield and quality is far greater. It seems
文摘Using genetic recombinant techniques, the tabtoxin resistant gene (trc gene) was to ligated a pBI121 vector under the CaMV 35S promoter to form the binary vector pBITR. Then the vector was introduced into Agrobacterium tumefaciens by triparental mating. After transforming the tobacco, many transgenic plants were obtained. PCR tests indicated that the trc gene was inserted in the genome DNA of tobacco. Biological activity analysis also demonstrated that the transgenic plant alleviated the symptom caused by the pathogen of tobacco wildfire disease.
