CO_2浓度倍增与干旱胁迫对油松(Pinus tabulaeformis)相对分枝级水力结构的影响

在密闭式生长箱内经过13个月高CO2浓度培养的5年生油松(Pinus tabulaeformis)为实验对象,采用改良冲洗法研究了CO2浓度倍增(720μmolmol-1)与干旱胁迫交互作用对油松相对分枝级水力结构参数的影响。通过测定油松不同分枝级的水力结构参数分别在720μmolmol-1CO2和380μmolmol-1CO2(大气现有CO2浓度)浓度下随着干旱胁迫的变化,得出不同分枝级的导水率(Kh)、比导率(Ks)和胡伯尔值(Hv)在2个CO2浓度下均随着干旱胁迫的增加而逐渐下降,叶比导率(Lsc)在720μmolmol-1CO2浓度下随着干旱胁迫的增加非线性变化(0级>2级>1级)不同于380μmolmol-1CO2(0级>1级>2级)。同期干旱胁迫条件下,720μmolmol-1CO2浓度下的Kh、Ks、Lsc和Hv均大于380μmolmol-1CO2且差异显著。根据整株苗木的水势将苗木的水分状况分为4个梯度,在正常水分(-0.45^-0.65MPa)、轻度干旱(-1.15^-0.75MPa)和中度干旱(-1.95^-1.35MPa)胁迫时,3个分枝级均在720μmolmol-1CO2条件下的Kh和Ks较380μmolmol-1CO2增加,说明交互作用能提高导水能力,同时加快水分运输效率。在重度干旱(<-2.80MPa)胁迫时Kh比380μmolmol-1CO2增加,而Ks比380μmolmol-1CO2减小,即交互作用提高了水分运输的安全性,却减少了有效性。

Effect of trace elements on growth of Pinus tabulaeformis seedling

The stimulative effect of trace elements on seed germination and seedling growth of Pinus tabulaeformis was tested. The experiments were carried out on seed soak and topdressing with different trace elements and varied concentrations at the nursery of Gardens Research Institute, Harbin, in 2000-2001. The experimental results showed that soaking seed with 1% and 0.2% concentrations of Mn element produced best result for seed germination, and the germination rate was increased by 9%~19% for the seeds treated with 1% concentration and 12%~14% for the seeds treated with 0.2% concentration compared with the control group. The seeds treated with boron element had lowest germination rate. For trace element topdressing, Mn and Mo elements presented good result for seedling growth and the treatment with low concentration was even better. The height or chlorophyll content of the seedlings with spray of low-concentration Mn and Mo element was much higher than that of untreated ones. In the contrast to the treating method of seed soak, topdressing (application of spraying on foliage) had evident effect on seedling growth.

Cytological Mechanism of Cytoplasmic Inheritance in Pinus tabulaeformis: Ⅱ. Transmission of Male and Female Organelles During Fertilization and Proembryo Development

In an earlier report the ultrastructure and nucleoid organelles of male gamete in Pinus tabulaeformis Carr. have been described. Presently, the ultrastructure of the cytoplasm of the egg cell and pollen tube—immediately before fertilization and during cytoplasmic transmission of male gametophyte—has been described for the same species. The fate of parental plastids and mitochondria in the proembryo has also been followed. The mature egg cell contains a large amount of mitochondria, but seems to lack normal plastids. Most plastids have transformed into large inclusions. Apart from the large inclusions, there are abundant small inclusions and other organelles in the egg cell. During fertilization, pollen tube penetrates into the egg cell at the micropylar end and thereafter the contents are released. Plastid and mitochondrion of male origin are lacking near the fusing sperm_egg nuclei. The second sperm nucleus—not involved in karyogamy—remains at a site near the receptive vacuole. This nucleus is surrounded by large amount of male cytoplasm containing mixed organelles from the sperm cell, tube cell, and egg cell. At the free nuclear proembryo stage, organelles of male and female origin are visible in the perinucleus_cytoplasmic zone. Most of the mitochondria have the same morphological features as those in the egg cell. Some of the mitochondria appear to have originated from the sperm and tube cells. Plastids are most likely of male gametophyte origin because they have similar appearance as those of the sperm and tube cell. Large inclusions in the egg cell become vacuole_like. Paternal plastids have been incorporated into the neocytoplasm of the proembryo. In the cellular proembryo, maternal mitochondria are more abundant. Plastids resembling those of the sperm and tube cell are still present. These cytological results clearly show that in P. tabulaeformis, plastids are inherited paternally and mitochondria bipaternally. The cytological mechanism of plastid and mitochondrion inheritance in gymnosperm is discussed.

Cytological Mechanism of Cytoplasmic Inheritance in Pinus tabulaeformis: Ⅰ. Ultrastructural Aspects and Nucleoids of the Male Gametes

The cytological mechanism of plastid and mitochondrion inheritance in Pinus is an interesting research topic with only a limited number of published articles. The results indicate that the sperms of Pinus tabulaeformis Carr. contain abundant plastids, mitochondria and organelle DNA. These data provide reliable cytological evidence of paternal plastid and mitochondrion inheritance in Pinus . The results are in line with the confirmed general rule of paternal plastid inheritance in Pinaceae. But whether mitochondria in sperm cells can be transmitted into the embryos is an issue needs further developmental studies. Another important finding is that contrary to earlier classification of the male gamete of Pinaceae into the male nuclei type, the results reveal that male gametes in Pinus tabulaeformis are actually cells. However, the sperm cells are only surrounded by plasma membranes without cell walls. The larger leading sperm cell in a pollen tube section is long in shape, with a large amount of cytoplasm; while the second sperm cell is smaller, round in shape and contains less cytoplasm. Whether this feature of the male gamete type could be considered as a representative characteristic of the family is discussed and further conclusions await more experimental evidences from studies on plants from different species.

Optimization of Ultrasound-assisted Extraction Conditions for Polysaccharides from Pinus tabulaeformis Seed Shells

[Objective] This study aimed to investigate the optimal technological condi- tions for ultrasouqd-assisted extraction of polysaccharides from Pinus tabulaeformis seed shells. [Melhod] Polysaccharides were extracted from P. tabulaeformis seed shells by ultrasound-assisted hot water extraction method and detected by phenol- concentrated sulfuric acid method. The effect of extraction temperature, extraction duration, solid-liquid ratio and extraction frequency on the extraction of polysaccha- rides was investigated using single-factor gradient experiment and the extraction conditions for polysaccharides from P. tabulaeformis seed shells were optimized us- ing L9（34） orthogonal design. [Result] The content of polysaccharides extracted twice at 60 ℃ for 25 min with solid-liquid ratio of 1：3.5 reached the maximum. The re- covery rate （n=-5） ranged from 97.71% to 100.67% and RSD was 1.56%, suggesting high stability. [Conclusion] A simple and efficient method for ultrasound-assisted ex- traction of polysaccharides from P. tabulaeformis seed shells was established in this study, which laid solid foundation for the development and utilization of abandoned pine nut shell resources.

Carbon,nitrogen and phosphorus stoichiometry in Pinus tabulaeformis forest ecosystems in warm temperate Shanxi Province,north China

Although carbon(C), nitrogen(N), and phosphorous(P) stoichiometric ratios are considered good indicators of nutrient excess/limitation and thus of ecosystem health, few reports have discussed the trends and the reciprocal effects of C:N:P stoichiometry in plant–litter–soil systems. The present study analyzed C:N:P ratios in four age groups of Chinese pine, Pinus tabulaeformis Carr., forests in Shanxi Province, China: plantation young forests(AY,<20 year-old); plantation middle-aged forests(AM, 21–30 year-old); natural young forests(NY,<30 year-old); and natural middle-aged forests(NM,31–50 year-old). The average C:N:P ratios calculated for tree, shrub, and herbaceous leaves, litter, and soil(0–100 cm) were generally higher in NY followed by NM,AM, and AY. C:N and C:P ratios were higher in litter than in leaves and soils, and reached higher values in the litter and leaves of young forests than in middle-aged forests;however, C:N and C:P ratios were higher in soils of middle-aged forests than in young forests. N:P ratios were higher in leaves than in litter and soils regardless of stand age; the consistent N:P<14 values found in all forests indicated N limitations. With plant leaves, C:P ratios were highest in trees, followed by herbs and shrubs, indicating a higher efficiency in tree leaf formation. C:N ratios decreased with increasing soil depth, whereas there was no trend for C:P and N:P ratios. C:N:P stoichiometry of forest foliage did not exhibit a consistent variation according to stand age. Research on the relationships between N:P, and P, N nutrient limits and the characteristics of vegetation nutrient adaptation need to be continued.

Anatomic Study of Female Sterility of Pinus tabulaeformis Carr.

The anatomic research on the mutant clone of Pinus tabulaeformis Carr. in the seed orchard in Xingcheng, Liaoning Province was carried out. The female cone of the mutant clone looked like normal, but its ovules degenerated in the early stage. This paper tries to find out the reason and time of ovule abortion. It seems that the ovule abortion is probably caused by female sterility because the microspores of this mutant clone were normal. Through the serial observations on the one-year-old macrosporangiates and the ovules of two-year-old female cones of mutant and normal clone, it is found that the reason of ovule abortion in mutant clone is the failure of the mitosis of free nuclei in the female gametophyte, and the time is about in the early April.

Content Changes of Several Endogenous Plant Hormones in Female-Sterile Pinus tabulaeformis Carr.

To determine the relationship between female sterility of Pinus tabulaeformis Carr. and plant hormones, content changes of several endogenous plant hormones in a female-sterile clone and a normal clone were analyzed during the key period of female gametophyte abortion in mutant clones. The change of ABA content showed a similar pattern between normal and mutant clones, but the ABA content of the mutant clone was always much higher than that of the normal clone. In the normal clone, the IAA content decreased significantly at the early stage of the period and reached its minimum during the first ten days of April and stabilized thereafter. The endogenous ZR levels increased sharply at the early stages and reached a maximum in the middle of April, then decreased dramatically. The IAA and ZR contents of the mutant clone did not change perceptively through the entire period. We conclude that the high levels of ABA and the deficit of IAA and ZR may be the reasons of female gametophyte abortion in the female-sterile clones of P. tabulaeformis.

Responses of Pinus tabulaeformis tree rings to climatic metrics in Hasi Mountain

A tree-ring width chronology developed from Pinus tabulaeformis tree-rings of Hasi Mountain was employed to analyze responses of tree-ring to different climatic metrics.Correlation analyses between tree-ring chronology and precipitation and temperature suggest that tree growth is both limited by precipitation and temperature,and moisture is the principal limiting factor of tree-ring width.To find a suitable moisture index to be reconstructed,three moisture indices considering precipitation and evaporation even water balance are chosen to be analyzed.The results suggest that the PDSI is an appropriate index as predictor of tree-ring width and can be used to reconstruct in this area.

Characteristics of flow production and sediment production of <i>Pinus tabulaeformis</i>through artificial rainfall simulation

In order to know well the relationship between vegetation and water in North China, especially Beijing, with exceptional water resources, we studied the characteristics of flow production and sediment production under different rainfall intensities by artificial rainfall simulation device. Results showed that increase of rainfall intensity would prolong the whole process of flow production, and vegetation on the slope would delay that process. Within the same duration, total runoff volume of each runoff plot and rainfall intensity had significant linear relationship. When vegetation kept unchanged, runoff velocity increased significantly with the increase of rainfall intensity, and owing to the formation of low permeable layer, the velocity increased fiercely during the early 3 minutes, reached stable at 10 - 15 minute. With the same rain intensity, total sediment yield decreased with rise of vegetation coverage, but increased obviously with rise of rain intensity and effectiveness of controlling sediment about 1 m x 1 m Pinus tabulaeformis stand decreased firstly and then increased, while that about 1.5 m x 1.5 m Pinus tabulaeformis stand kept decreasing. Since the tags with A, B and C for 0.42 mm/min, 0.83 mm/min, 1.29 mm/min, order of sediment concentration of wasteland plot was B > C > A, and 1 m x 1 m Pinus tabulaeformis plot B > A > C. Through this study, some suggestions were expected to be provided for water balance of Beijing area and certain basis for construction of shelter forest.

Climate response and radial growth of Pinus tabulaeformis at different altitudes in Qilian Mountains

In order to test whether the relationship between climate and the radial growth of trees is affected by altitude,altitude variability and time stability of climate-influenced radial growth of a dominant conifer,Chinese pine(Pinus tabulaeformis),in the eastern Qilian Mountains were studied against the background of climate change using dendrochronology.Results show that 1)droughts at the end of the growing season of last year and during the early and middle growing season of the current year were the main limiting factors for the radial growth of Chinese pine at two altitude gradients;this was determined by analyzing the relationship between tree-ring width chronologies and climate factors.2)The sensitivity of the radial growth of trees to climate change gradually decreased and was affected more by drought stress at a lower altitude.3)An unstable divergence response was observed in the radial growth at the two altitudes,in response to controlling climatic factors;this observation was based on the moving correlation analysis of growth/climate relationships,and the aggravation of drought stress caused by increasing temperature was the main reason.4)The growth rate of Chinese pine at the two altitudes increased at first and then decreased,as measured by basal area increment(BAI)modeling.Future temperature rises may have significant effects on mountain forest ecosystems in arid and semi-arid regions.Effective management and protection measures should be taken,according to the response patterns of trees to climate change at different altitude gradients.

Analysis of the Volatile Constituents of PinusTabulaeformis Carr. Pine Cones

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Study on the production effect and prevention index of the forthputting of sonsau cocuus sinensis chen on the Pinus tabulaeformis

The growth of Pinus tabulaeformis is influenced by the sonsau cocuus sinensis chen. When the insect density is more than 300-350 head/hundred beam conifer, sonsau cocuus sinensis chen occurred significantly influence the Pinus tabulaeformis＇ s growth. when the insect density reached 250-300 head/100 beam coniferous, sonsau cocuus sinensis chen significantly influence the accumulation of Pinus tabulaeformis volume. To determine the sonsau cocuus sinensis chen 2nd instar nymph theory control index was 220.5 / hundred beam needles has a guiding significance to the production practice.

Spatial patterns of Pinus tabulaeformis and Pinus massoniana forests in Qinling-Daba Mountains and the boundary of subtropical and warm temperate zones

The Qinling Mountains is not only the geographical boundary between North and South China,but also the boundary between subtropical and warm temperate zones.It plays an important role in the geo-ecological pattern of China.However,there is controversy about the specific location of this geographical boundary in academic community due to the complexity,transition and heterogeneity of the transitional zone,as well as the differences in the delimitation indicators and research purposes.To further reveal the characteristics of the North-South transitional zone and clarify the specific location of the geo-ecological boundary between North and South China,combined with SRTM topographic data,temperature and precipitation data,Pinus massoniana forest and Pinus tabulaeformis forest,which represent subtropical coniferous forest in South China and temperate coniferous forest in North China respectively,were chosen to analyze their spatial distributions in the Qinling-Daba Mountains and the climatic conditions at their boundary with the climatic indexes of annual precipitation,the coldest month(January) average temperature,the warmest month(July) average temperature and the annual average temperature.The results show that:(1) Pinus massoniana and Pinus tabulaeformis forests and the climate indicators of their boundary can be used as one of the vegetation-climate indexes for the delimitation of subtropical and warm temperate zones.The boundary between the subtropical coniferous forest(Pinus massoniana forest) and temperate coniferous forest(Pinus tabulaeformis forest) is located along the south slope of Funiu Mountain to the north edge of Hanzhong Basin(the south slope of Qinling Mountains) at an altitude of 1000–1200 m,where the climatic indictors are stable:the annual precipitation is about 750–1000 mm,the annual average temperature is about 12–14℃,the coldest monthly average temperature is 0–4℃,and the warmest monthly average temperature is about 22–26℃.(2) It can be more scientifically to delimitate the boundary of subtropical and warm temperate zones in China by comprehensively considering the vegetation-climate indicators.Additionally,the boundary between subtropical and warm temperate zones in Qinling-Daba Mountains should be a transitional zone consisting of the boundaries of coniferous forests,broad-leaved forests and shrubs between subtropical and warm temperate zones.The results provide a scientific basis for the selection of delimitation index of subtropical and warm temperate zones.

Effects of environmental humidity on the survival and development of pine caterpillars, Dendrolimus tabulaeformis （Lepidoptera： Lasiocampidae）

The pine caterpillar, Dendrolimus tabulaeformis is one of the most important pests on Pinus tabulaeformis and other pine species in North China. In the present study, effects of relative humidity （RH） on the development and survival of pine caterpillars and soil moisture （SM） on their diapausing larvae were investigated. Low RH （20%） deferred the development of eggs and larvae, reduced egg hatching and larval surviving compared to 40%, 60% and 80% RH. Both low （20%） and high （100%） RH reduced egg hatching, but only 20% RH deferred the development of larvae, prolonged developmental duration and reduced the body mass and body length of larvae. The SM influenced the survival of diapausing larvae significantly. The dry treatment significantly reduced the supercooling points （SCPs）, whereas increased the mortality and reduced body mass from 56.9 to 36.5 mg and body water content from 78% to 63% after 2 weeks＇ exposure. Therefore, higher RH is more favorable for the development of early instars and survival of diapausing larvae of the pine caterpillars.

Changes in Germination and Physiological Characteristics of Seeds of Robinia pesudoacacia and Pinus tabulaeformis during Artificial Ageing

In order to study seed quality changes of main afforestation species under high temperature and high relative humidity, the deterioration mechanism of seeds of Robinia pesudoacacia and Pinus tabulaeformis from arid and semiarid areas of Northern China was elucidated in this study. The seeds were artificially aged for 2 and 6 d at the temperature of 45 oC and the relative humidity (RH) of 50%, 75% and 100% , respectively. The results showed that the germinability decreased and the cell membrane deteriorated with the artificial ageing. The decrease in germinability was well correlated with the decreased activities of dehydrogenase and α-amylase. After 6-day accelerated ageing, the seed germination and the activities of dehydrogenase and α-amylase decreased to a great extent for both species of seeds. The results suggest that seed deterioration during accelerated ageing is closely related to the increase of relative conductivity and the decrease of dehydrogenase and α-amylase activities.

Discussion on Pipe Model through Hydraulic Architecture of Pinus tabulaeformis Seedling

In the paper, the hydraulic architecture parameters of Pinus tabulaeformis seedlings (4 years old)were measured by improved flushing method under normal water condition in the green house and the basictheory of hydraulic architecture is used to discuss the rationality of the pipe model. The results of theexperiment and simulation show that the differences of hydraulic conductivity, specific conductivity and leafspecific conductivity is great in different stems and branches of Pinus tabulaeformis seedlings. The hydraulicconductivity of non-constriction area is higher than that of constriction area. The devotion of functionalxylem of stem to unit leaf growth is not a constant, namely, the Huber value is diverse. Even though the pipemodel has been accepted in some areas, its precondition is not perfect, and it is helpless in correctlyunderstanding the essence of water transport in seedlings from the prospective of water physiology.

基于树轮宽度重建的河南石人山地区1850年以来季节最低温度及20世纪增温（英文）

气候变化影响农业生产和相应的管理活动。中原地区位于我国中部,地域广阔,是我国重要的粮食生产基地。了解这一地区的历史气候变化极为重要。目前我们对这一地区过去几百年气候变化历史的了解主要来自于历史文献记载,缺乏自然地质记录的证据。本研究从河南石人山地区获取了一组油松树轮样本,在树轮宽度和现代气象观测记录统计相关分析的基础上,建立转换方程,重建了石人山地区1850—2011年上年10月至当年6月的平均最低温度。重建对观测温度(1958—2011年)的解释方差达到48.8%。重建中较暖时段有1892—1906年和1995—2011年;较冷期主要发生在1875—1890年、1910—1932年和1967—1980年。石人山地区自20世纪10年代以来持续增温,20世纪80年代以后增温更为显著(重建的10个高温年中9个都出现在1998年之后),表明石人山地区的温度重建结果能够捕捉20世纪以来的全球变暖信号。树轮宽度和温度的正相关关系也表明如果石人山地区未来持续变暖,将为油松的生长创造有利条件。本文重建中的冷暖时段与研究区附近其他地区温度序列中的冷暖时段较为一致,表明我国中部温度变化在较大区域范围内较为同步。空间场分析进一步证实了上述分析。此外,重建温度与全球海温(SST)的空间场分析表明石人山地区温度变化与西太平洋海温、印度洋和北大西洋海温变化密切相关。

Transformation and assessment of Chinese pine pure plantations for soil and water conservation in western Liaoning Province,P. R. China

Western Liaoning Province is characterized by huge areas of lowly-efficient Chinese pine (Pinus tabulaeformis Carr.) pure plantations. How to transform these plantations has become an increasingly significant management problem. In this study, the authors summarized the approaches, which are based on close-to-nature silvicultural system, to transform the pure pine plantations. Native broadleaved trees were planted in three methods: 1) after strip clearcutting, 2) after patch clearcutting; 3) on the open forestland and the forest edge. The transformation targets and the selection of tree species were expatiated in this paper. The key techniques and their application conditions for each method were analyzed and discussed. Through investigation and contrastive analysis, the assessment was made to the stands transformed by strip method. Results showed that the mixed stands at 16 years after transformation had an obvious layered structure and the species richness of understorey vegetation increased by 23.5%–52.9%. Soil enzyme activities of urease, phosphatase and sucrase increased by 6%–142%, 46%–99% and 31%–200%, respetively. Moreover, the transformed stands could effectively control the occurrence of pine caterpillars in plantations. Consequently the transformations enhanced the function of soil and water conservation. Keywords Pinus tabulaeformis - Monoculture - Transformation - Principles and methods - Assessment CLC number S791.254 - S727.22 Document code A Foundation item: This research was supported by grants from the Chinese Academy of Sciences (KZCX3-SW-418), National Natural Science Foundation of China (30100144), and National Key Technologies R & D Program of China (96-007-01-06).Biography: ZENG De-hui (1965-), male, Ph.D. professor in the Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, P. R. ChinaResponsible editor: Zhu Hong

4种油松针叶DNA提取方法的比较

采用SDS法、简易提取法、高盐沉淀法和改良的CTAB法从油松(Pinus tabulaeformis)针叶提取基因组DNA,通过琼脂糖凝胶电泳、紫外分光光度法和PCR检测提取的DNA质量。结果表明,改良的CTAB法提取的油松针叶基因组DNA浓度和纯度高,适合用于ISSR-PCR分析,优于其他3种提取方法。