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猪带绦虫(Taenia solium)囊尾蚴发育过程的超微结构观察 被引量:9
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作者 赵艳兵 李庆章 郝艳红 《华中农业大学学报》 CAS CSCD 北大核心 2005年第6期614-617,共4页
系统地研究了猪囊尾蚴发育过程中超微结构的变化。5头仔猪,分别经口服感染3节猪带绦虫孕卵节片,于感染后不同时期剖杀,剥离囊尾蚴。取囊壁和头颈部,作透射电镜观察。观察结果表明,随感染时间的延长,猪囊尾蚴细胞或组织的类型未发生明显... 系统地研究了猪囊尾蚴发育过程中超微结构的变化。5头仔猪,分别经口服感染3节猪带绦虫孕卵节片,于感染后不同时期剖杀,剥离囊尾蚴。取囊壁和头颈部,作透射电镜观察。观察结果表明,随感染时间的延长,猪囊尾蚴细胞或组织的类型未发生明显变化,只在大小或发育程度上有所增加。头节和囊壁均由皮层和实质区构成,但二者的超微结构有差别。头节肌肉较发达,皮层外表面的微毛较短,基质区线粒体较少,实质区糖原颗粒不及囊壁贮存多,并且排泄管也较少。提示二者可能执行不同的功能:囊壁与营养吸收有关,而头节可能与将来的发育有关。 展开更多
关键词 猪囊尾蚴 发育 超微结构
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首次在中国发现圈养环尾狐猴感染肥头绦虫
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作者 刘芸江 杨金朋 +5 位作者 刘俊卿 陈善瑜 刘海峰 周紫峣 钟志军 彭广能 《野生动物学报》 北大核心 2024年第3期648-652,共5页
首次在中国报道1例由长颈囊尾蚴(Cysticercus longicollis)引起的5岁雄性圈养环尾狐猴(Lemur catta)皮下囊尾蚴病,患病动物表现为明显的臀部囊肿,但无其他异常体征。该病例就诊于四川农业大学第二教学动物医院。在就诊期间,采用细针穿... 首次在中国报道1例由长颈囊尾蚴(Cysticercus longicollis)引起的5岁雄性圈养环尾狐猴(Lemur catta)皮下囊尾蚴病,患病动物表现为明显的臀部囊肿,但无其他异常体征。该病例就诊于四川农业大学第二教学动物医院。在就诊期间,采用细针穿刺对囊肿内容物进行形态学检查。结果显示:囊肿内含有大量囊尾蚴,典型囊尾蚴呈椭圆形,具有长而可伸缩的颈部和单个头节,头节上有4个吸盘和16个(单排)小钩。通过对囊尾蚴DNA进行nad1和cox1基因扩增及测序,进一步确定该囊尾蚴为肥头绦虫(Taenia crassiceps)的幼虫形态,即长颈囊尾蚴,研究结果可为该寄生虫在国内的流行病学调查及防控提供参考。 展开更多
关键词 肥头绦虫 环尾狐猴 长颈囊尾蚴
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猪带绦虫(Taenia solium)囊尾蚴发育过程中的周围宿主反应 被引量:1
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作者 刘永杰 郝艳红 李庆章 《上海农业学报》 CSCD 2003年第1期84-86,共3页
首次系统地研究了猪囊尾蚴发育过程中的周围宿主反应。结果显示 ,宿主炎性细胞在囊尾蚴周围出现有先后顺序。初期以嗜中性粒细胞、嗜酸性粒细胞和淋巴细胞为主 ,随后巨噬细胞及上皮样细胞开始出现 ,并在虫体周围呈围墙样增生 ,最后在虫... 首次系统地研究了猪囊尾蚴发育过程中的周围宿主反应。结果显示 ,宿主炎性细胞在囊尾蚴周围出现有先后顺序。初期以嗜中性粒细胞、嗜酸性粒细胞和淋巴细胞为主 ,随后巨噬细胞及上皮样细胞开始出现 ,并在虫体周围呈围墙样增生 ,最后在虫体外围形成结缔组织包囊。从病理组织学结果可以看出 。 展开更多
关键词 绦虫 囊尾蚴 发育过程 周围宿主反应 嗜酸性粒细胞
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国医大师张志远运用竹茹经验
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作者 田迎春 刘桂荣 《山东中医杂志》 2024年第3期290-294,共5页
介绍国医大师张志远教授应用竹茹的经验。张老临床知药而用,遣方用药时常突出竹茹的用量,通过将其与诸药配伍,以更好地发挥竹茹清热泻火、降逆止呕、解郁安神的功效。同时善用经方、创制经验方治疗多种杂病、顽证,如四皓汤治疗“灯笼病... 介绍国医大师张志远教授应用竹茹的经验。张老临床知药而用,遣方用药时常突出竹茹的用量,通过将其与诸药配伍,以更好地发挥竹茹清热泻火、降逆止呕、解郁安神的功效。同时善用经方、创制经验方治疗多种杂病、顽证,如四皓汤治疗“灯笼病”、三竹汤治疗神经衰弱、南阳理胃汤治疗顽固性呕哕,方中突出竹茹的地位,常作为君药发挥作用。附验案3则。 展开更多
关键词 国医大师 张志远 竹茹 四皓汤 三竹汤 南阳理胃汤
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一例牛带绦虫与蛔虫混合感染的诊治回顾并文献复习
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作者 谭臻 杜中瑜 +1 位作者 龙丽梅 乐慧星 《科技与健康》 2024年第6期9-12,共4页
为探讨牛带绦虫与蛔虫驱虫治疗方法,对患者自体逸出孕节及随粪便排出孕节进行病原学检查;对患者排出粪便用直接法和改良加藤厚涂片法检测,通过对粪便虫卵进行检查和肉眼对光观察虫体孕节的子宫分支及墨汁染色进行鉴定。从患者粪便直接... 为探讨牛带绦虫与蛔虫驱虫治疗方法,对患者自体逸出孕节及随粪便排出孕节进行病原学检查;对患者排出粪便用直接法和改良加藤厚涂片法检测,通过对粪便虫卵进行检查和肉眼对光观察虫体孕节的子宫分支及墨汁染色进行鉴定。从患者粪便直接涂片和改良加藤厚涂片发现带绦虫卵及未受精蛔虫卵,判定为带绦虫和蛔虫的混合感染。结合孕节子宫分支情况及流行病学调查,将该带绦虫确定为牛带绦虫感染。采用南瓜子-槟榔治疗带绦虫,一个月后建议患者用阿苯达唑进行蛔虫驱虫治疗,并长期随访。研究发现,患者本次感染带绦虫的原因为食用了未熟透的牛肉,蛔虫感染原因为患者喜食生菜或不良卫生习惯及饮食习惯。因此,人们要注意饮食方式,讲究卫生,避免肠道寄生虫病发生;应加强健康教育,改变人们不良的饮食习惯,相关部门要加大对生肉的监督检疫力度。 展开更多
关键词 牛带绦虫 混合感染 鉴定 治疗
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Investigation Food Diet of Cobitis taenia in Talar River (Mazandaran Province)
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作者 Soheil Bahrebar 《Journal of Food Science and Engineering》 2012年第12期697-704,共8页
Perch sweeper fish with scientific name Cobitis taenia is one of the Cobitidae family. Since this kind of fish lives in clean waters and in the high areas of Talar River, it can be an index on water implution. This su... Perch sweeper fish with scientific name Cobitis taenia is one of the Cobitidae family. Since this kind of fish lives in clean waters and in the high areas of Talar River, it can be an index on water implution. This survey on the diet of this specimen is done on two stations (Kaselian and Tajoon) and shallow and high areas of Talar River in Mazandaran (Southern part of Caspian sea) and the duration of this survey is from October 2008 till September 2009. We should mention that fishing was just done by electro-shocker with voltage 300 V. And this fish after fishing is fixation in 10% formalin solution. So 410 perch sweeper fish were examined and the average of relatively length of gut (RLG) was 17.35 cm. The index of vacated intestine (VI) was calculated 43.35% for all samples. From intense of diet points of view (GSI), the most frequency of full intestines was considered in summer and spring. On the other hand, the smallness of relatively length of gut index (RLG 〈 1) shows that perch sweeper fish's diet manner is inclined to herbivority. In the examination of containing of digestive track, overally 10 kinds of foodstuff were known. Based on amount of this index, 2.67% Chironomidae is known as main bait in all sampling period. 展开更多
关键词 Perch sweeper fish (Cobitis taenia) DIET Mazandaran Talar River.
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王济华运用角药治疗类风湿关节炎经验 被引量:1
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作者 王荷珺 王子华 杜明瑞 《河南中医》 2024年第3期351-355,共5页
王济华教授认为,类风湿关节炎早期多因外感风、寒、湿三邪,三者之间相互促进、互相转化,风寒表邪入里日久或郁而化热;病程日久多虚实夹杂,肝肾亏虚、气血不足,同时兼有寒湿痹阻、痰瘀互结。故王教授治疗痹证早期以祛邪散寒为主,兼顾清... 王济华教授认为,类风湿关节炎早期多因外感风、寒、湿三邪,三者之间相互促进、互相转化,风寒表邪入里日久或郁而化热;病程日久多虚实夹杂,肝肾亏虚、气血不足,同时兼有寒湿痹阻、痰瘀互结。故王教授治疗痹证早期以祛邪散寒为主,兼顾清热利湿、化痰散结,中晚期则偏于滋养气血、补益肝肾。王济华教授运用角药治疗痹证颇有心得,组方精妙、配伍严谨,常用角药川芎-延胡索-白芍行气活血、柔筋止痛;忍冬藤-络石藤-青风藤清热通络、祛风除湿;半夏-陈皮-竹茹燥湿化痰、理气和中;白术-薏苡仁-茯苓益气健脾、利湿消肿;桑寄生-续断-杜仲滋补肝肾、强筋壮骨;酸枣仁-合欢皮-首乌藤宁心安神、解郁除烦。王教授临证时,追求阴阳调和平衡、气机升降有度,临床用药有药简力专、增效减负之功。 展开更多
关键词 类风湿关节炎 角药 川芎-延胡索-白芍 忍冬藤-络石藤-青风藤 半夏-陈皮-竹茹 白术-薏苡仁-茯苓 桑寄生-续断-杜仲 酸枣仁-合欢皮-首乌藤 王济华
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41例肠道寄生带绦虫taenia微生态学初步观察
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作者 蔡之宣 于小明 蔡云 《中国微生态学杂志》 CAS CSCD 1995年第5期55-58,共4页
1986年作者在西双版纳傣族自治洲,选择41例肠道内寄生带绦虫者,驱虫进行微生态学观察。初步结果表明该地区带绦虫病有如下特点:(1)定植率高,占26%,当地有生食牛、猪肉的习惯;(2)存在牛、猪带绦虫混合感染。41例... 1986年作者在西双版纳傣族自治洲,选择41例肠道内寄生带绦虫者,驱虫进行微生态学观察。初步结果表明该地区带绦虫病有如下特点:(1)定植率高,占26%,当地有生食牛、猪肉的习惯;(2)存在牛、猪带绦虫混合感染。41例中驱出带绦虫者37例,其中18例寄生牛带绦虫,15例寄生猪带绦虫,4例牛、猪带绦虫混合感染;(3)在感染有猪带绦虫19例(包括混合感染的4例)中,9例为多条寄生,占47%(9/19),3条以上者7例,占多条定植的7/9。发现1例一次排出猪带绦虫16条,实为罕见,而且这16条虫体大小,平均体长达2.7m;(4)19例猪带绦虫感染者均不伴囊尾蚴寄生及囊虫病的临床表现史,这与已往文献报告有所不同。 展开更多
关键词 肠道寄生虫 带绦虫 微生态学
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Molecular differentiation of cryptic stage of Echinococcus granulosus and Taenia species from faecal and environmental samples
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作者 Diganta Pan Sumanta De +3 位作者 Asit Kumar Bera Subhashis Bandyopadhyay Subrata Kumar Das Debasis Bhattacharya 《Asian Pacific Journal of Tropical Medicine》 SCIE CAS 2010年第4期253-256,共4页
Objective:To differentiate cryptic stage of Echinococcus granulosus(E.granulosus) and Taenia by PCR-RFLP and sequence information of amplicon.Methods:DNA were isolated from metacestodes stage of Taenia and E.granulosu... Objective:To differentiate cryptic stage of Echinococcus granulosus(E.granulosus) and Taenia by PCR-RFLP and sequence information of amplicon.Methods:DNA were isolated from metacestodes stage of Taenia and E.granulosus using DNA isolation kit(Q-BIOgene kit,USA), the amplified and purified DNA product was then cloned and sent for sequencing.The generating sequence information was used for amplicons identification.Results:Out of 112 faecal and environmental samples,16 exhibited positive result.The product size of amplicon positive for E.granulosus was 310 bp;whereas,for Taenia spp.sizes varied from 379 to 388 bp.Restriction pr of ile of actinⅡwith Csp61 also differed Taenia spp.and E.granulosus.Conclusions:The result of the study indicated that,the primers were useful to differentiate cryptic stage of the two genera which is yet to be reported earlier. 展开更多
关键词 taenia solium ECHINOCOCCUS granulosus Environmental SAMPLES PCR-RFLP
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Evaluation of activity of triclabendazole against Taenia solium metacestode in naturally infected pigs
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作者 Ana Vargas-Calla Luis A.Gomez-Puerta +5 位作者 Juan Calcina Omar Gonzales-Viera Cesar Gavidia Maria T.Lopez-Urbina Hector H.Garcia Armando E.Gonzalez 《Asian Pacific Journal of Tropical Medicine》 SCIE CAS 2016年第1期20-23,共4页
Objective:To assess the efficacy of triclabendazole(TCBZ) in porcine cysticercosis.Methods:Eighteen naturally infected cysticercotic pigs were divided into 3 groups of 6 individuals each.The first group was treated or... Objective:To assess the efficacy of triclabendazole(TCBZ) in porcine cysticercosis.Methods:Eighteen naturally infected cysticercotic pigs were divided into 3 groups of 6 individuals each.The first group was treated orally with TCBZ at a single dose of 30 mg/kg of body weight,the second group was treated orally with oxfendazole at a single dose of 30 mg/kg of body weight and the third group received a placebo(control group).All animals were kept under the same management conditions.The pigs were euthanized 17 wk post-treatment and the number of surviving cysts in muscles was assessed and compared between groups.Results:All pigs treated with oxfendazole had only degenerated cysts in their carcasses.In contrast,TCBZ had very little effect against the parasitic cysts.Cysts from pigs in the TCBZ group looked apparently normal after treatment.However,histological evaluation showed a mild to moderate degree of inflammation.Conclusions:TCBZ is not an efficacious drug against Taenia solium cysticercosis in swine using a single dose. 展开更多
关键词 taenia solium CYSTICERCOSIS TRICLABENDAZOLE OXFENDAZOLE Treatment
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Oxfendazole as successful treatment of Taenia hydatigena metacestodes in naturally infected pigs
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作者 Luis Antonio Gomez-Puerta Armando Emiliano Gonzalez +4 位作者 Cesar Gavidia Viterbo Ayvar Hector Hugo Garcia Maria Teresa Lopez-Urbina for the Cysticercosis Working Group in Peru 《Asian Pacific Journal of Tropical Biomedicine》 SCIE CAS 2015年第11期971-973,共3页
The efficacy of oxfendazole(OFZ) on Taenia hydatigena metacestodes, also called Cysticercus tenuicollis(C. tenuicollis), was studied in 648 raising pigs. This study was performed in Tumbes Department in Peru, an endem... The efficacy of oxfendazole(OFZ) on Taenia hydatigena metacestodes, also called Cysticercus tenuicollis(C. tenuicollis), was studied in 648 raising pigs. This study was performed in Tumbes Department in Peru, an endemic area for cysticercosis. Pigs were randomized in two groups; untreated group(n = 142) did not receive any treatment and treated group(n = 506) received OFZ treatment at a single dose of 30 mg/kg body weight.Six months after treatment, the pigs were necropsied. The prevalence of infection by C. tenuicollis among the pigs was 27.5%(39/142) and 2.0%(10/506) in untreated and treated groups, respectively. Untreated group was infested only with viable cysts, whereas treated group had no viable cysts. All the cysts found in treated group presented degeneration, with a thick membrane, and they contained milky fluid and fibrous tissue. A single dose of OFZ was effective against C. tenuicollis, thus providing an alternative drug for controlling this parasite in pigs. 展开更多
关键词 taenia hydatigena CYSTICERCUS tenuicollis Oxfendaz
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Taeniasis:A possible cause of ileal bleeding
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作者 Alessia Settesoldi Alessandro Tozzi Ottaviano Tarantino 《World Journal of Clinical Cases》 SCIE 2017年第12期432-436,共5页
Taenia spp.are flatworms of the class Cestoda,whose definitive hosts are humans and primates.Human infestation(taeniasis) results from the ingestion of raw meat contaminated with encysted larval tapeworms and is consi... Taenia spp.are flatworms of the class Cestoda,whose definitive hosts are humans and primates.Human infestation(taeniasis) results from the ingestion of raw meat contaminated with encysted larval tapeworms and is considered relatively harmless and mostly asymptomatic.Anemia is not recognized as a possible sign of taeniasis and taeniasis-induced hemorrhage is not described in medical books.Its therapy is based on anthelmintics such praziquantel,niclosamide or albendazole.Here we describe a case of acute ileal bleeding in an Italian man affected with both Taenia spp.infestation resistant to albendazole and Helicobacter pylori-associated duodenal ulcers. 展开更多
关键词 taenia spp. INTESTINAL BLEEDING Irondeficiency ANEMIA Endoscopy
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Unusual colonoscopy finding:Taenia saginata proglottid
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作者 Nayan M Patel Eric L Tatar 《World Journal of Gastroenterology》 SCIE CAS CSCD 2007年第41期5540-5541,共2页
Infection with tapeworms is a major problem in many parts of the world. Patients may be asymptomatic or have a significant morbidity depending on the species. Infection with Taenia species is sometimes found by expuls... Infection with tapeworms is a major problem in many parts of the world. Patients may be asymptomatic or have a significant morbidity depending on the species. Infection with Taenia species is sometimes found by expulsion of eggs or proglottids in stool. Species specific diagnosis of Taenia is difficult,but possible. We present a case of Taenia saginata incidentally discovered,and risk factors for transmission,diagnosis,symptoms,and treatment. 展开更多
关键词 taenia saginata COLONOSCOPY TAPEWORM
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青藏高原田鼠源多刺带绦虫囊尾蚴的鉴定及遗传进化分析 被引量:1
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作者 张学勇 简莹娜 +8 位作者 朵红 郭志宏 马霄 张青 赵存哲 郭帅 马万里 李志 付永 《中国预防兽医学报》 CAS CSCD 北大核心 2023年第11期1193-1198,共6页
为鉴定我国青藏高原分离的田鼠源绦虫囊尾蚴的种类及其遗传进化特征,本研究通过对该囊尾蚴进行形态学观察,采用绦虫cox1基因通用引物,利用PCR扩增并测序,初步鉴定该囊尾蚴。形态学观察结果显示,该囊尾蚴为小型虫体,虫体背部和腹部稍扁平... 为鉴定我国青藏高原分离的田鼠源绦虫囊尾蚴的种类及其遗传进化特征,本研究通过对该囊尾蚴进行形态学观察,采用绦虫cox1基因通用引物,利用PCR扩增并测序,初步鉴定该囊尾蚴。形态学观察结果显示,该囊尾蚴为小型虫体,虫体背部和腹部稍扁平,长度约为200.0 mm,虫体横截面直径约为2.5 mm;虫体分为3部分:前端部分直径通常略小,中间部分表面为皱褶区域,约占整个虫体的一半,后端部分细长,相对光滑,有平行的横向凹陷;PCR结果显示,在约440 bp处出现与预期目的片段大小相符的条带,测序结果显示,该基因序列与多刺带绦虫(T.polyacantha)cox1基因序列(EU544581)的同源性高达97.0%。初步表明该囊尾蚴属于多刺带绦虫囊尾蚴。采用多刺带绦虫线粒体cox1、nad1和12S rRNA特异性基因引物经PCR进一步鉴定并测序。将3个基因测序结果与GenBank登录的多刺带绦虫的相应基因序列比对,分析它们之间的同源性,并构建这3个基因的进化树,分析它们之间的遗传进化关系。PCR结果显示,分别扩增到约390 bp(cox1)、480 bp(nad1)和240 bp(12S rRNA)的目的条带。同源性分析结果显示,3个基因序列与GenBank登录的多刺带绦虫相应基因序列的同源性最高分别达99.4%、98.7%和100.0%。进化树结果显示,该虫株的3个基因均与不同宿主的多刺带绦虫处于同一分支。本研究在国内首次鉴定到青藏高原田鼠源多刺带绦虫囊尾蚴,并分析其线粒体基因的同源性及遗传演化关系,为多刺带绦虫的分类、鉴定和种群遗传进化分析及分子流行病学调查等奠定了研究基础。 展开更多
关键词 多刺带绦虫 囊尾蚴 田鼠 鉴定 遗传进化
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Preparation of Taenia solium Stained Specimens and Morphological Observation
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作者 Yang Yicheng Zhang Guoquan +4 位作者 Zhang Yuanlai Liu Lijun Zhang Liang Tian Qihui Zhang Jinlong 《Animal Husbandry and Feed Science》 CAS 2017年第4期195-196,203,共3页
[ Objective ] In order to observe the morphological structure of Taenia solium and improve the dyeing method of Taenia solium. [ Method ] Taenia soli- um was stained by hematoxylin-eosin (HE) staining method and obs... [ Objective ] In order to observe the morphological structure of Taenia solium and improve the dyeing method of Taenia solium. [ Method ] Taenia soli- um was stained by hematoxylin-eosin (HE) staining method and observed under microscope. Taenia solium specimens with clear structure and bright color were made. [ Results] Male reproductive system and excretory system of Taenia solium segment could be observed under the optical microscope, structures of uterus and eggs in Taenia solium gravid proglottid could also be observed. [ Conclusion] Aiming at providing a reference for the identification of Taenia solium and the diagnosis of the tapeworm disease. 展开更多
关键词 taenia solium Hematoxylin staining MORPHOLOGY
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<i>Taenia solium</i>Cysticercosis in Sub-Saharan Africa: Perspectives for a Better Control
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作者 Beda John Mwang’onde 《Advances in Infectious Diseases》 2019年第2期105-121,共17页
For decades, Taenia solium cysticercosis (TSC) has remained an important constraint for public health and economic well being of the predisposed communities. Despite the fact that the disease is easily preventable, hu... For decades, Taenia solium cysticercosis (TSC) has remained an important constraint for public health and economic well being of the predisposed communities. Despite the fact that the disease is easily preventable, humans remain foci for transmission. They harbour the mature form of the parasite (Taenia solium) which may be curable at this stage and also easily prevented from contaminating pig feeds and environment with eggs of the parasite to break the cycle. Researchers, knowledgeable communities and international organisations such as the World Health Organisation work hard to make the disease a history. Nevertheless, persistence of the disease in endemic regions such as sub Saharan Africa is kept on increasing. While TSC in human may serve as a silent killer as it can go unnoticed for years, the immanent social cultural settings and lifestyles of the communities in endemic areas form the main concern on the epidemiology of the disease. Unless lifestyles change in endemic regions, front line research might rarely find its way into applications in an area of huge societal and economic impact, the TSC epidemiology. Sincerely, transmission of TSC is favoured by poor sanitation and hygiene which is motivated by lifestyle and poverty. What is perhaps missing is the knowledge on “why is the communities involved ease indiscriminately while almost every member of the community in endemic areas knows that indiscriminate defecation may harm their health? What social drivers are behind this motive (bush defecation)? How best can the disease be controlled and monitored? This review recommends for developing and implementing an interdisciplinary OneHealth community-based control mechanism and monitoring (surveillance) programme as standpoints towards eliminating TSC in sub-Saharan Africa and the rest endemic regions. 展开更多
关键词 taenia solium CYSTICERCOSIS EPIDEMIOLOGY Sub Saharan AFRICA
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猪带绦虫丝氨酸蛋白酶抑制分子Ts-serpin-2对人THP-1细胞炎性细胞因子的表达影响 被引量:1
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作者 毕研丽 刘仲藜 +3 位作者 郭爱疆 张少华 王帅 才学鹏 《中国动物传染病学报》 CAS 北大核心 2023年第1期16-25,共10页
为了深入研究猪带绦虫丝氨酸蛋白酶抑制分子(Ts-serpin-2)对THP-1细胞炎性细胞因子分泌的调节作用,本实验通过RTPCR从猪带绦虫成虫扩增获得Ts-serpin-2(TsM_000807300)的编码序列CDS,利用毕赤酵母表达Ts-serpin-2重组蛋白。利用发色底... 为了深入研究猪带绦虫丝氨酸蛋白酶抑制分子(Ts-serpin-2)对THP-1细胞炎性细胞因子分泌的调节作用,本实验通过RTPCR从猪带绦虫成虫扩增获得Ts-serpin-2(TsM_000807300)的编码序列CDS,利用毕赤酵母表达Ts-serpin-2重组蛋白。利用发色底物特异性反应检测Ts-serpin-2蛋白对猪胰蛋白酶、胰弹性蛋白酶、牛α-糜蛋白酶、猪胃蛋白酶、木瓜酶、凝血酶和组织蛋白酶G的抑制效果。重组蛋白Ts-serpin-2处理THP-1细胞24 h,应用qRT-PCR和ELISA方法检测各炎性细胞因子差异表达水平。结果显示,真核酵母表达的重组蛋白Ts-serpin-2分子量约为48 kDa,具有蛋白酶抑制活性,对牛α-糜蛋白酶、猪胰弹性蛋白酶及猪胰蛋白酶的活性均有明显的抑制作用。重组蛋白Ts-serpin-2可抑制LPS活化的THP-1细胞促炎性细胞因子IL-1β、IL-6、IL-12α、IFN-γ和iNOS2 mRNA的转录水平,促进IL-4刺激的THP-1细胞抗炎性细胞因子IL-10的表达。ELISA结果显示,Ts-serpin-2能够抑制巨噬细胞分泌致炎因子TNF-α、IL-6和IFN-γ,刺激IL-10的分泌,与qRT-PCR检测结果基本一致。上述研究结果表明猪带绦虫Ts-serpin-2可通过调节宿主巨噬细胞分泌的炎性因子影响虫体入侵过程中宿主的免疫应答。 展开更多
关键词 猪带绦虫 丝氨酸蛋白酶抑制分子 真核表达 THP-1细胞 炎性细胞因子
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“人体寄生虫学”课程思政教学改革方法探究 被引量:2
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作者 李菲 《教育教学论坛》 2023年第14期157-160,共4页
根据国家针对高等学校教育的指示,结合人体寄生虫学的学科特点,对“人体寄生虫学”课程思政改革进行了探究和分析,并以《猪带绦虫》章节的微课为例,详细介绍了“人体寄生虫学”课程思政教学改革的具体思路和方法。通过深入挖掘和提炼“... 根据国家针对高等学校教育的指示,结合人体寄生虫学的学科特点,对“人体寄生虫学”课程思政改革进行了探究和分析,并以《猪带绦虫》章节的微课为例,详细介绍了“人体寄生虫学”课程思政教学改革的具体思路和方法。通过深入挖掘和提炼“人体寄生虫学”课程中蕴含的思政元素,将其融入专业教育过程,使专业知识传授与价值引领和能力培养相结合,对帮助医学生更好地理解和掌握人体寄生虫学专业知识、培养学生的专业素养和综合能力等都具有重要意义。 展开更多
关键词 人体寄生虫学 猪带绦虫 课程思政 教学改革
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Expect the Unexpected: Bowel Obstruction Caused by <i>Taenia saginata</i>: A Case Report of Unusual Complications
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作者 Muhaned Alhassan Ayman Ibraheim Barghash Hatem Al-Saadi 《Case Reports in Clinical Medicine》 2021年第7期191-196,共6页
<strong>Background:</strong> Small bowel obstruction (SBO) is a common gastrointestinal condition often warranting acute surgical intervention. In human, tapeworm species can cause a parasitic infection by... <strong>Background:</strong> Small bowel obstruction (SBO) is a common gastrointestinal condition often warranting acute surgical intervention. In human, tapeworm species can cause a parasitic infection by ingestion of raw or under cooked beef (<em>T. saginata</em>) or pork (<em>T. solium</em> and <em>T. asiatica</em>). Taeniasis usually presents with vague symptoms or mild abdominal pain and discomfort. The intestinal complications are not commonly mentioned in the literatures.<strong> Case presentation:</strong> We present 65-year-old Egyptian woman who lives in Oman, who is known to have type two diabetes mellitus and hypertension. She presented to Emergency department with abdominal pain and constipation for a period of three days. On examination, she was dehydrated with tachycardia (pulse rate: 110/minute) blood pressure of 100/60 mmHg and patient was afebrile. Abdominal examination revealed, mildly distended abdomen and tense, no guarding or rigidity no palpable mass, exaggerated bowel sound and rectum is empty on per rectal examination. Emergency laparotomy was performed with intra-operative findings of large foreign body impacted in the distal jejunum about 8 × 3 cm, cylinder in shape completely obliterating the lumen, distal loops was collapsed. <strong>Conclusion: </strong>Although Taeniasis is a very rare infection, index of suspicion such as a potential in the differential diagnosis as cause of an acute abdomen with unusual surgical complications should be considered. The diagnosis of these rare circumstances is usually made intraoperatively, and surgery is recommended only for the treatment of complications. 展开更多
关键词 TAPEWORM CYSTICERCUS taenia saginata Intestinal Obstruction Proglottids
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不同类型绦虫感染的临床表现特征比较分析 被引量:1
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作者 李小丽 栗绍刚 吴赵永 《传染病信息》 2023年第2期119-123,153,共6页
目的总结并分析带绦虫与裂头绦虫感染的临床表现及实验室指标变化特征。方法对在北京友谊医院2016年1月—2022年7月就诊的28例肠道绦虫病患者资料进行总结,其中带绦虫病组12例,裂头绦虫病组16例,并分析比较2组患者的临床特点及实验室指... 目的总结并分析带绦虫与裂头绦虫感染的临床表现及实验室指标变化特征。方法对在北京友谊医院2016年1月—2022年7月就诊的28例肠道绦虫病患者资料进行总结,其中带绦虫病组12例,裂头绦虫病组16例,并分析比较2组患者的临床特点及实验室指标差异。结果2组患者平均年龄分别为(29.33±15.42)岁和(28.88±11.57)岁。裂头绦虫病组的无症状者比例(10/16)大于带绦虫病组(4/12),2组中均有4例患者表现为腹痛、腹胀症状。带绦虫病组中,2例患者表现为肛周瘙痒,1例患者表现为体质量下降,但裂头绦虫病组中,患者均无上述2种症状。带绦虫病组中2例(2/12)患者粪便中检测到带绦虫卵,而裂头绦虫病组则有7例(7/16)患者检测到虫卵。28例患者的粪便中均检测到节片。带绦虫病组的北京本地病例为7例(7/12),裂头绦虫病组为10例(10/16)。除2例患者外,其他患者均有食用生或半生可疑牛肉或鱼肉的流行病学史。结论2种绦虫感染的患者临床症状均轻微,多无症状,但仍有发生严重并发症及2种不同种类寄生虫共感染的风险,流行病学史的询问可提高该疾病的诊断率。 展开更多
关键词 带绦虫病 裂头绦虫病 猪带绦虫 牛带绦虫 阔节裂头绦虫 临床特点 流行病学 食源性寄生虫病
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