Vitis amurensis is a valuable resource for wine production. Ripening of the grape berry is the key phase which determines the com- position of wine. To better understand the gene expression that manifest in V. amurens...Vitis amurensis is a valuable resource for wine production. Ripening of the grape berry is the key phase which determines the com- position of wine. To better understand the gene expression that manifest in V. amurensis berry skins during the ripening, cDNA library of V. amurensis berry skins was constructed. A total of 935 high quality ex- pressed sequence tags （ESTs） were obtained from the library. These ESTs represent 636 unigenes, including 108 contigs and 528 singletons. The EST analysis was performed and genes were assigned to functional categories according to their primary BLAST match. Of these 25.35% were involved with metabolism, 6.27% with cell rescue and defense, 6.84% energy, 11.68% protein synthesis, 18.8% protein activity regula- tion, 11.11% cell structure, 7.98% transport, 6.27% transcription and the remaining 5.7% were signal transduction. The generated ESTs were characterized by the gene ontology analysis and were categorized ac- cording to its cellular component, molecular function and biological process. In the cDNA library, some genes are relevant to the biosynthesis of anthocyanins, while some genes are related to grape berry maturation.展开更多
目的构建亚洲带绦虫成虫全长cDNA质粒文库,为获取亚洲绦虫成虫的基因信息,建立基因表达谱,并为筛选疫苗基因和诊断抗原基因奠定基础。方法提取亚洲带绦虫成虫mRNA,构建pBluescript II SK全长cDNA质粒文库,测定扩增文库的滴度;用载体克...目的构建亚洲带绦虫成虫全长cDNA质粒文库,为获取亚洲绦虫成虫的基因信息,建立基因表达谱,并为筛选疫苗基因和诊断抗原基因奠定基础。方法提取亚洲带绦虫成虫mRNA,构建pBluescript II SK全长cDNA质粒文库,测定扩增文库的滴度;用载体克隆位点两端的引物进行PCR扩增,以检测所构建文库的质量。随机挑选质粒文库转化的阳性重组克隆,进行较大规模的5′端测序,归并unigene。结果测定文库的滴度为1011pfu/μl,插入片段的大小主要在1000bp以上。共测1495个克隆,获得有效EST序列1126个,归并为643条unigene。结论已成功获得一高质量的亚洲带绦虫成虫全长cDNA表达文库,并获得了较丰富的成虫表达基因数据。展开更多
基金supported by the China Agriculture Research System (CARS-30)
文摘Vitis amurensis is a valuable resource for wine production. Ripening of the grape berry is the key phase which determines the com- position of wine. To better understand the gene expression that manifest in V. amurensis berry skins during the ripening, cDNA library of V. amurensis berry skins was constructed. A total of 935 high quality ex- pressed sequence tags （ESTs） were obtained from the library. These ESTs represent 636 unigenes, including 108 contigs and 528 singletons. The EST analysis was performed and genes were assigned to functional categories according to their primary BLAST match. Of these 25.35% were involved with metabolism, 6.27% with cell rescue and defense, 6.84% energy, 11.68% protein synthesis, 18.8% protein activity regula- tion, 11.11% cell structure, 7.98% transport, 6.27% transcription and the remaining 5.7% were signal transduction. The generated ESTs were characterized by the gene ontology analysis and were categorized ac- cording to its cellular component, molecular function and biological process. In the cDNA library, some genes are relevant to the biosynthesis of anthocyanins, while some genes are related to grape berry maturation.
文摘目的构建亚洲带绦虫成虫全长cDNA质粒文库,为获取亚洲绦虫成虫的基因信息,建立基因表达谱,并为筛选疫苗基因和诊断抗原基因奠定基础。方法提取亚洲带绦虫成虫mRNA,构建pBluescript II SK全长cDNA质粒文库,测定扩增文库的滴度;用载体克隆位点两端的引物进行PCR扩增,以检测所构建文库的质量。随机挑选质粒文库转化的阳性重组克隆,进行较大规模的5′端测序,归并unigene。结果测定文库的滴度为1011pfu/μl,插入片段的大小主要在1000bp以上。共测1495个克隆,获得有效EST序列1126个,归并为643条unigene。结论已成功获得一高质量的亚洲带绦虫成虫全长cDNA表达文库,并获得了较丰富的成虫表达基因数据。
