Do alternative stable states exist in large shallow Taihu Lake,China?

Regime shifts from submersed macrophyte dominance to phytoplankton dominance have been widely reported in small-to medium-sized shallow lakes.However,alternative stable states in large shallow lakes(surface area>500 km^(2))remain unconfirmed.To understand the alternative stable states and the main influencing factors of submersed macrophytes in large lakes,the ecosystem states from monitoring data from 1959 to 2019 in large shallow Taihu Lake(2338 km^(2)in average depth of 2.12 m)in China were examined.Changes in submersed macrophyte coverage(C_(Mac))and phytoplankton chlorophyll a(Chl a)in the time series and their relationships with environmental factors were analyzed.During the field investigation from August 2018 to May 2019,nutrients and Chl a showed obvious heterogeneity across the lake,being generally higher in the western and northern areas and lower in the southeast area,while C_(Mac)was only observed in the eastern areas,e.g.,East Taihu Lake,Xukou Bay,and Gonghu Bay.During the long-term monitoring from 1959 to 2019 in the Central Region,Meiliang Bay,and East Taihu Lake,Chl a increased significantly in the time series.C_(Mac)varied slightly among different subareas,always at low levels(<10%)in the Central Region and Meiliang Bay but at relatively high levels in East Taihu Lake(10%–90%).Frequency distributions of response variables had no multimodality except for C_(Mac)in East Taihu Lake,with two peaks between 15%and 20%and between 55%and 60%.A dual relationship was found between Chl a and total phosphorus(TP)in the areas with and without macrophytes,while C_(Mac)showed no relationship with TP,and submersed macrophytes did not flourish in the Central Region and Meiliang Bay even when TP was at very low levels(≈10 mg/m3).Taihu Lake had similar algal turbidity(TurbAlg)as small-to mediumsized lakes but generally presented with higher values of nonalgal turbidity(TurbNonAlg),as did their contribution to total turbidity as a percentage.This study suggested that large shallow Taihu Lake may have no alternative stable states,but more evidence is needed for East Taihu Lake,which was dominated by macrophytes,as it remains unknown whether hysteresis occurs between the processes of eutrophication and oligotrophication.Unfavorable conditions caused by wind might be the main reason due to the absence of submersed macrophytes in Taihu Lake.These results demonstrate that stricter nutrient control is needed to maintain a healthy state or to recover from a decayed state for large lakes.

Phosphorus fractionation and bio-availability in Taihu Lake(China) sediments

Typical sediments from Taihu Lake, a meso-to-hypereutrophic lake, were collected and examined on the basis of P-fractionation by sequential extraction scheme. Sedimentary inorganic phosphorus were fractioned into four forms and the rank order according to the mean concentration of P-fractions in Taihu Lake was NaOH-P>BD-P>HCl-P>NH_4Cl-P. The concentrations of BD-P were linearly correlated with the content of active Fe(R2=0.96). Also, the linear relationship between the sum of BD-P and NaOH-P and the sum of active Fe and active Al content was observed within the six sediments investigated(R2=0.96). Moreover, the bio-available phosphorus(BAP) content was estimated by the sum of NH_4Cl-P, BD-P, and NaOH-P, viz. BAP=NH_4Cl-P+NaOH-P+BD-P. In Taihu Lake, the BAP contents are ranging from 0.10 mg/g dw to 1.25 mg/g dw, and average 0.40 mg/g dw for all sediment samples. The relative contributions of BAP to total sedimentary phosphorus(TP) and inorganic sedimentary phosphorus(IP) range from 18.67% to 50.79%(33.61% on average) and from 52.82% to 82.09%(67.81% on average), respectively.

Effects of hydrodynamics processes on phosphorus fluxes from sediment in large,shallow Taihu Lake

The turnover of phosphorus （P） in lake sediments, a major cause of eutrophication and subsequent deterioration of water quality, is in need of deep understanding. In this study, effects of resuspension on P release were studied in cylindrical microcosms with Yshape apparatus. The results indicated that there was a positive correlation between flux of suspended substance across sediment-water interface （Fss） and the wind speed, and an increasing Fss during each wind process followed by a steady state. The maximal Fss under fight, moderate, and strong wind conditions were 299.9±41.1,573.4±61.7, and 2093.8±215.7 g/m^2, respectively. However, flux of P across sediment-water interface （Fp） did not follow a similar pattern as Fss responding to wind intensity, which increased and reached the maximum in initial 120 rain for fight wind, then decreased gradually, with maximal flux of 9.4±1.9 mg/m^2. A rapid increase of Fp at the first 30 rain was observed under moderate wind, with maximal flux of 11.2±0.6 mg/m^2. Surprisingly, strong wind caused less Fp than under light and moderate wind conditions with maximal flux of 3.5±0.9 mg/m^2. Fss in water column declined obviously during the sedimentation process after winds, but Fp varied with wind regime. No obvious difference was detected on Fp after 8 h sedimentation process, compared with the initial value, which means little redundant P left in the water column after winds.

Bioaccumulation of heavy metals in fishes from Taihu Lake,China

The Cr, Zn, Cu, Cd, Pb contents were determined in Cyprinus carpio Linnaeus, Carassius auratus Linnaeus, Hypophthalmichthys molitrix and Aristichthys nobilis, which were caught from Meiliang Bay, Taihu Lake, a large, shallow and eutrophic lake of China. The results showed that： （1） the Cr, Cu, Pb, Cd contents in the edible parts of the four fish species were much lower than Chinese Food Health Criterion （1994）, but the Zn contents were higher than the Criterion; （2） Cd contents were the highest in the liver of fish, Pb contents were almost the same in all organs of fish, Cr contents mainly enriched in the skin and gonads, Zn contents were the highest in the gonad （♀）, and Cu contents were the highest in the liver; （3） the total metal accumulation was the greatest in the liver and the lowest in the muscle. The total metal accumulation was the highest in C. auratus L. This investigation indicated that fish products in Taihu Lake were still safe for human consumption, but the amount consumed should be controlled under the Chinese Food Health Criterion to avoid excessive intake of Zn.

Three-dimensional eutrophication model and application to Taihu Lake,China

Taihu Lake, the largest freshwater shallow lake in eastern China, has suffered from severe eutrophication over the past two decades. This research developed a three-dimensional eutrophication model to investigate the eutrophication dynamics. The model fully coupled the biological processes and hydrodynamics, and also took into account the effects of sediment release and the external loads from the tributaries. After sensitivity analyses, the key parameters were defined and then calibrated by the field observation data. The calibrated model was applied to study the seasonal primary productions and its regional differences. The comparisons between model results and field data in year 2000 indicated that the model is able to simulate the eutrophication dynamics in Taihu Lake with a reasonable accuracy. From the simulation experiments, it was found that the meteorological forcing have significant influences on the temporal variations of the eutrophication dynamics. The wind-induced circulation and sediment distribution play an important role in the spatial distribution of the algae blooms.

Composition analysis of colored dissolved organic matter in Taihu Lake based on three dimension excitation-emission fuorescence matrix and PARAFAC model, and the potential application in water quality monitoring

Taihu Lake is one of the five biggest lakes in China. Surface water samples from 26 sampling sites of Taihu Lake were collected. Furthermore wet chemical analysis （CODCr and BOD5） and measurement of three dimensional excitation-emission matrix （3DEEM） spectra in the laboratory have been conducted. Using parallel factor analysis （PARAFAC） model, three components of colored dissolved organic matter （CDOM） have been identified successfully, based on the analysis of 3DEEM data. The characteristics of the three components also have been described by comparing them to some components of CDOM, identified in earlier researches. Meanwhile, spatial variations of concentration for the three components in Taihu Lake have been analyzed, and the result indicates that the concentration of component 1 depends more on the situation of wastewater pollution and can be used as the indicator of wastewater pollution. The relationship between the concentrations of the three components and results of the wet chemical analysis show that none of the three components can be used as indicators of gross organic matter in water. However, the concentrations of all the three components have obvious linear relationships with the BOD5 value, especially for component 1 （r = 0.72878）. Finally, the potential applications of the composition analysis based on 3DEEM and PARAFAC model in water quality monitoring have been illuminated.

Sediment distribution pattern mapped from the combination of objective analysis and geostatistics in the large shallow Taihu Lake, China

Investigation was made into sediment depth at 723 irregularly scattered measurement points which cover all the regions in Taihu Lake, China. The combination of successive correction scheme and geostatistical method was used to get all the values of recent sediment thickness at the 69×69 grids in the whole lake. The results showed that there is the significant difference in sediment depth between the eastern area and the western region, and most of the sediments are located in the western shore-line and northern regimes but just a little in the center and eastern parts. The notable exception is the patch between the center and Xishan Island where the maximum sediment depth is more than 4.0 m. This sediment distribution pattern is more than likely related to the current circulation pattern induced by the prevailing wind-forcing in Taihu Lake. The numerical simulation of hydrodynamics can strong support the conclusion. Sediment effects on water quality was also studied and the results showed that the concentrations of TP, TN and SS in the western part are obviously larger than those in the eastern regime, which suggested that more nutrients can be released from thicker sediment areas.

Heavy-metal contents in suspended solids of Meiliang Bay,Taihu Lake and its environmental significances

Surface water was taken from river mouth to the central area of Meiliang Bay, Taihu Lake, a large shallow eutrophic lake in China. Suspended solids were condensed by centrifugation 25 L surface water samples from each selected site. Suspended solids and surface sediments were further freeze-dried and microwave digested before determining the metals by ICP-AES. Among the metals analyzed in suspended solids and sediments, contents of Cr, Cu, Mn, Ni, and Zn in suspended solids were significantly higher than those in sediments while contents of Al, Ba, Be, Ca, Co, Fe, K, Mg, Pb, and V in suspended solids were 10%—30% higher than those in sediments. Sr and Ti contents in suspended solids and sediments were very similar. Na content in suspended solids was lower than that in sediments. Heavy metals were significantly accumulated in suspended solids. From the river mouth to the center of Meiliang Bay, contents of Cr, Cu, Pb, and Zn in suspended solids showed a gradual decreasing trend indicating the river(Zhihugang River) still discharged large quantity of heavy metals to Meiliang Bay. The study suggests that the geochemical behaviors and ecological effects of heavy metals in suspended solids may serve as a good indicator for the pollution of lake.

DYNAMIC VARIATIONS OF WATER QUALITY IN TAIHU LAKE AND MULTIVARIATE ANALYSIS OF ITS INFLUENTIAL FACTORS

Dynamic variation of water quality in Meiliang Bay and part of West Taihu Lake has been analysed based on data from 1991 to 1992. Principal Component Analysis is used to reveal the mutual relationships of various factors. It is shown that there existis an obvious spatial and temporal variation in the main factors of water quality. Annual values of TP, CON, TN, Chl-a and conductivity decrease evidently from inner Meiliang Bay to the outer from north to south. TP and TN fluctuate seasonally with much higher value in winter. This is particularly true for the mouth of Liangxi River. In addition, the Chl-1 has a synchronous variation with water temperature, although being lagged a little, and closely relates to TP and TN. Finally, the results from Principal Component Analysis show that TP, TN, SS (or SD), water temperature and Chl-a are the most influential factors to water qualuty in this area, and both suspensions and algae can contribute to transparency to Taihu Lake.

Impact assessment of climate change and human activities on annual highest water level of Taihu Lake

The annual highest water level of Taihu Lake （Zm） is very significant for flood management in the Taihu Basin. This paper first describes the inter-annual and intra-annual traits of Zm from 1956 to 2000. Then, using the Mann-Kenall （MK） and Spearman （SP） nonparametric tests, the long-term change trends of area precipitation and pan evaporation in the Taihu Basin are determined. Meanwhile, using the Morlet wavelet transformation, the fluctuation patterns and change points of precipitation and pan evaporation are analyzed. Also, human activities in the Taihu Basin are described, including land use change and hydraulic project construction. Finally, the relationship between Zm, the water level of Taihu Lake 30 days prior to the day of Zm （Z0）, and the 30-day total precipitation and pan evaporation prior to the day of Zm （P and E0, respectively） is described based on multi-linear regression equations. The relative influence of climate change and human activities on the change of Zm is quantitatively ascertained. The results demonstrate that： （1） Zm was distinctly higher during the 1980-2000 period than during the 1956-1979 period, and the 30 days prior to the day of Zm are the key phase influencing Zm every year; （2） P increased significantly at a confidence level of 95% during the 1956-2000 period, while the reverse was true for E0; （3） The relationship between Zm, P and E0 distinctly changed after 1980; （4） Climate change and human activities together caused frequent occurrences of high Zm after 1980; （5） Climate change caused a substantially greater Zm difference between the 1956-1979 and 1980-2000 periods than human activities. Climate change, as represented by P and E0, was the dominant factor raising Zm, with a relative influence ratio of 83.6%, while human activities had a smaller influence ratio of 16.4%.

Phragmites australis and Typha orientalis in removal of pollutant in Taihu Lake, China

Two plant populations of Phragmites australis and Typha orientalis grown in gravel and sediment substrate were studied to assess their capabilities for purifying polluted water in Taihu Lake, China. The substrate displayed most significant effects on the suspended matter （P 〈 0.01）, with the reduction of 76%-87% and 52%--63% for P. australis, and 83%-86% and 45%-62% for T. orientalis in gravel substrate and sediment substrate, respectively. Both species and substrates significantly decreased the N and P concentrations of water body （P 〈 0.01）. P. australis showed higher total N and P concentrations in tissues than T. orientalis and had a greater potential to remove nutrients from the lake. Phosphate was easily to concentrate in the belowground tissues, while nitrate concentration was higher in leaf and stalk. Therefore, harvesting the aboveground tissues could take most of nitrate out of the sediment. The saturate photosynthetic rate （Asat） of P. australis was higher than that of T. orientalis when grown in sediment substrate. But instance water-use- efficiency （WUEi） （A/E） and intrinsic water use efficiency （A/gs） showed the maximum values of two species grown in river water. With significant difference in gs, however, intercellular CO2 concentration （Ci） had no obvious difference in two species which indicated that high Asat value of P. australis might result from the increased carboxylation capacity of the mesophyll, because of the central role of N in photosynthetic enzymes. Our findings suggest that the plants could absorb most of nitrogen in polluted water, while gravel displayed a high capacity for absorbing the suspended matters and phosphate salts. Therefore, biological and physiological pathways for pollutant removal should be integrated.

Impact of Taihu Lake on City Ozone in the Yangtze River Delta

The lake-breeze at Taihu Lake generates a different specific heat capacity between the water body and the surrounding land. Taihu Lake has a significant impact on the atmospheric conditions and the air quality in the Yangtze River Delta. This phenomenon is referred to as the Taihu Lake effect. In this study, two simulations were conducted to determine the impact of the Taihu Lake effect in the reference experiment （R-E） and sensitivity experiments （NO_TH）. The control simulations demonstrated that the meteorological field and the spatial distribution of ozone （03） concentrations over Taihu lake obviously changed once the land-use type of water body was substituted by cropland. The surface temperature of Taihu Lake was reduced under the impact of Taihu Lake, and a huge temperature difference caused a strong lake-breeze effect. The results also showed that the difference in the average concentrations of 03 between the R-E and NO_TH experiments reached 12 ppbv in most areas of Taihu Lake, all day, on 20 May 2014. During daytime （0800-1600 LST, LST=UTC＋8）, the influence of the Taihu Lake effect on 03 in the Suzhou region was not significant. However, the influence of the Taihu Lake effect on 03 in the Suzhou region was obvious during nighttime （1800-2400 LST）. The larger changes in the physical and chemical processes were horizontal and vertical advections under the influence of the Taihu Lake effect in Taihu Lake.

Divergence of carbon dioxide fluxes in different trophic areas of Taihu Lake,China

Carbon dioxide partial pressures(pCO 2) and CO 2 fluxes on air water interface in different trophic level areas of Taihu Lake were calculated and corrected using alkalinity, pH, ionic strength, active coefficient, water temperature and wind speed on the basis of the data sets of monthly sampling in 1998 The mean values of pCO 2 in the hypertrophic, eutrophic, and mesotrophic areas are 1807 8±1025 8(mean±standard deviation) μatm, 416 3±207 8 μatm, and 448 5±194 0 μatm,respectively. A maximum and minimum pCO\-2 values were found in the hypertrophic(4053 7 μatm) and the eutrophic(3 2 μatm) areas. There was about one magnitude order of difference in mean CO\-2 fluxes between the hypertrophic area(27 3±17 4 mmol/(m\+2·d)) and the eutrophic(1 99±4 50 mmol/(m\+2·d)) and mesotrophic (2 22±4 31 mmol/(m\+2·d)) areas. But there was no significant difference between eutrophic and mesotrophic areas in pCO 2 and the flux of CO 2 In respect to CO 2 equilibrium, input of the rivers will obviously influence inorganic carbon distribution in the riverine estuary. An exponential relationship between the pCO 2 values and chlorophyll a concentrations was obtained( r =0 8356, n =60) in eutrophic bay. Results suggested that lake ecosystems, also may be considered as unique aggregation, which can contain and be patient of different components that have their relative independence so long as its size enough to large. A productive lake, though it has positive fluxes of CO 2 to atmosphere during the most of time, is a huge and permanent sink of carbon in terrestrial ecosystems through receiving a great quantity of carbon materials via rivers, precipitation, and biological production.

Maintaining healthy rivers and lakes through water diversion from Yangtze River to Taihu Lake in Taihu Basin

On the basis of the Taihu water resources assessment, an analysis of the importance and rationality of the water diversion from the Yangtz,e River to Taihu Lake in solving the water problem and establishing a harmonious eco-environment in the Taihu Basin is performed. The water quantity and water quality conjunctive dispatching ＇decisi＂ofi-makifig support system, which ensures flood control, water supply and eco-aimed dispatching, is built by combining the water diversion with flood control dispatching and strengthening water resources monitoring and forecasting. With the practice and effect assessment, measures such as setting the -integrated basin management format, further developing water diversion and improving the hydraulic engineering projects system and water monitoring system are proposed in order to maintain healthy rivers and guarantee the development of the economy and society in the Taihu Basin.

Effects of temperature and nutrients on phytoplankton biomass during bloom seasons in Taihu Lake

Long-term variations of phytoplankton chlorophyll-a （Chl-a）, nutrients, and suspended solids （SS） in Taihu Lake, a large shallow freshwater lake in China, during algal bloom seasons from May to August were analyzed using the monthly investigated data from 1999 to 2007. The effective accumulated water temperature （EAWT） in months from March to June was calculated with daily monitoring data from the Taihu Laboratory for Lake Ecosystem Research （TLLER）. The concentrations of Chl-a and nutrients significantly decreased from Meiliang Bay to Central Lake. Annual averages of the total nitrogen （TN）, total phosphorus （TP）, and Chl-a concentrations, and EAWT generally increased in the nine years. In Meiliang Bay, the concentration of Chl-a was significantly correlated with EAWT, ammonia nitrogen （NH4＋N）, TN, the soluble reactive phosphorus （SRP）, TP, and SS. In Central Lake, however, the concentration of Chl-a was only correlated with EAWT, TP, and SS. Multiple stepwise linear regression revealed that EAWT, dissolved total phosphorus （DTP）, and TP explained 99.2% of the variation of Chl-a in Meiliang Bay, and that EAWT, NH4＋-N, and TP explained 98.7% of the variation of Chl-a in Central Lake. Thus EAWT is an important factor influencing the annual change of phytoplankton biomass. Extreme climate change, such as extremely hot springs or cold springs, could cause very different bloom intensities in different years. It is also suggested that both nutrients and EAWT played important roles in the growth of phytoplankton in Taihu Lake. The climate factors and nutrients dually controlled the risk of harmful algal blooms in Taihu Lake. Cutting down phosphorus and nitrogen loadings from catchments should be a fundamental strategy to reduce the risk of blooms in Taihu Lake.

Analysis of black water aggregation in Taihu Lake

Black water aggregation （BWA） in Taihu Lake is a disaster for the lake environment. It is a phenomenon resulting from water environmental deterioration and eutrophication caused by accumulation of pollutants in the lake, according to research on the water quality, pollutants of BWA, and occurrence mechanisms of BWA. Dead algae are the material base of BWA, the polluted sediment is an important factor for the formation of BWA, and hydrological and meteorological conditions such as sun light, air temperature, wind speed, and water flow are the other factors that may lead to the formation of BWA. Thioether substances such as dimethyl trisulfide are the representative pollutants of BWA. Parameters such as chlorophyll-a, DO, pH, and water temperature are sensitive indicators of BWA. Measures such as algae collection, ecological dredging, pollution control, and water diversion from the Yangtze River to the lake, are effective, and strengthening aeration is an emergency measure to control BWA.

Identification and Determination of Microcystins in Source Water and Waterbloom Sample From Meiliang Bay,Taihu Lake,China

Objective To identify and determine the congener and level of microcystins in the source water of Taihu Lake. Methods Improved method of SPE combined with HPLC was employed to detect the concentration and varieties of microcystins in source water and bloom samples collected from Meiliang Bay, Taihu Lake. Results The contents of two predominant microcystin components, MC-RR, and MC-LR, were relatively high in samples during warm months and correlated with the phase of algae growth. The maximum concentrations of MC-RR and MC-LR in water sample reached 3.09±0.53μg/L and 2.39±0.41μg/L during the period of water bloom in September 2004, respectively. Even without waterbloom, the concentration of MC-LR in source water sample was still higher than the guideline value. Conclusion The status of microcystin pollution in this region is serious and measures to monitor and control the growth of cyanobacteria are urgently needed.

Responses of soil nutrient to driving mechanism in Taihu Lake basin in last 20 years

The objectives of this study is to understand the changing law of soil nutrient quality in Taihu Lake basin, Yangtze Delta in last 20 years, and reveal the relationship between soil nutrient changes and human driving forces in regional level over long period of time. Experiment on long-term collecting samples was conducted to study changes of soil organic matter, total N, available N, available P and available K in Dongting town, Wuxi city during the last 20 years. The paper analyses the co-relationship of the fluctuation of soil nutrient and state policy, cultivating practice, agricultural input, and per hectare agricultural net income. The results showed that at first the content of soil organic matter increased, then decreased, and increased again. The content of total N and available N steadily increased. The content of available K and available P steadily decreased. The authors found that the influence of state policy and cultivating practice on soil nutrient quality index (SNQI) is obvious, agricultural input and SNQI are positively correlated, and per hectare agricultural net income and SNQI are negatively correlated.

A model for the retrieval of suspended sediment concentrations in Taihu Lake from TM images

A study was conducted in the Taihu Lake with the aim of deriving a model for the retrieval of suspended sediment （SS） concentrations from Landsat TM images and in situ sampled data. The correlation between suspended sediment concentrations of lake and the reflectance obtained from the TM images is significant. By TM images and in situ sampled data in summer and winter, we obtained a comparative uniform model for the retrieval of suspended sediment concentrations in the Taihu Lake, that is lnSS = a＊（R3/R1） ＋ b, where lnSS is the natural logarithm of the suspended sediment concentration, Rl and R3 are the reflectance coincident with the 1st band and the 3rd band in TM images, a and b are the regression coefficients. Furthermore, we analysed the errors particularly to make sure the model is valid. The model is accurate to within 0.33（RMSE）, suggesting that this model may be applicable to predict suspended sediment in the Taihu Lake from TM image throughout the year.

General Thinkingon the Management of Blue Algae Outbreak in the Taihu Lake,the Chaohu Lake and the Dianchi Lake

Among the Chinese lakes with the problem of eutrophication, the Taihu Lake, the Chaohu Lake and the Dianchi Lake have the worst cases of blue algae outbreak, which happen every year. Other lakes also have problems of blue algae outbreak at various degrees. However, some lakes don＇t have such problems. Practices have shown that through comprehensive management, the problem can be basically eliminated or significantly alleviated. ＂Water bloom＂ and blue algae outbreak have different connotation. The major factors affecting blue algae outbreak are sources of pollutants and ecological environment. Experiences are summed up and a new thinking on its management is developed： resolving the problem of blue algae is fundamental to lake management and development goals should be clearly set forth so as to eventually build a healthy aquatic ecosys- tem. The problem of blue algae can＇t be fully tackled by solely relying on the management of eutrophication but only by combining efforts of reducing the amount of blue algae and the management of eutrophication. The number of reduced blue algae should be larger than that of naturally prolifera- ted algae so as to alleviate and eliminate the problem of blue algae outbreak. Various engineering and technical measures and relevant protective procedures should be carried out in a scientific and proper manner. The total amount of nitrogen and phosphorous entering the lake either from point-source or non-point source pollution should be substantially reduced. Controlling the source of pollution and intercepting pollutants are the bas- ic measures. Wastewater treatment plant is the largest point source pollution in the future and adequate plants should be built with improved emission standards. Meanwhile, other measures including fishing out blue algae, diverting water, dredging, ecological restoration and expanding reed zone, should be implemented to alleviate the problem of eutrophication and eventually eliminate the problem of blue algae outbreak.