No or little information on the use fresh(wet)housefly maggots(Musca domestica)in African catfish(Clarias gariepinus)fry feeding.Therefore,this study was conducted to investigate the effect of feeding on fresh(wet)hou...No or little information on the use fresh(wet)housefly maggots(Musca domestica)in African catfish(Clarias gariepinus)fry feeding.Therefore,this study was conducted to investigate the effect of feeding on fresh(wet)housefly maggots with or without artificial diet on water quality,growth performance,survival percentage and feed utilization of African catfish fry under laboratory conditions.Housefly maggots produced from a mixture of poultry droppings and foods wastes,it was used to replace artificial feed at 0,50 and 100% levels.Catfish were fed artificial diet alone(Feed 1),fresh(wet)housefly maggots alone(Feed 2),and 50% fresh housefly maggots with 50% artificial diet(Feed 3)were prepared and tested on triplicate groups of African catfish fry(initial weight of 0.25±0.02 g)for 60 days.Results showed that final weight(g/fish)was significantly(P≤0.05)higher in fish fed on feed 3(6.03±0.08),followed by fish fed feed 2(4.62±0.27),followed by fish fed feed 1(3.15±0.68).Specific growth rate(%/day)was also significantly higher in fish fed on feed 3(5.31±0.10),followed by fish fed feed 2(4.86±0.03),followed by fish fed feed 1(4.18±0.24).The same trend was observed with total weight gain,percentage weight gain,daily growth rate and relative growth rate.Feed intake and protein intake were significantly(P≤0.05)higher in fish fed on feed 3 and fish fed on feed 2,followed by fish fed feed 1.While,feed conversion ratio(FCR)and protein efficiency ratio were not significantly(P>0.05),but the improvement in FCR recorded in catfish fry fed feed 3 and feed 2 under the experimental conditions.Survival percentage was within the range 55-75%,with insignificant differences(P>0.05)among treatments.The water quality parameters such as temperature,pH,dissolved oxygen,total ammonia,nitrite and nitrate were not significantly(P>0.05)between the treatments and were tolerable for Catfish culture.Accordingly,use of the 50% fresh(wet)housefly maggots with 50% artificial diet in African catfish fry feeding had positive effect on growth performance and reduce of the feed cost.展开更多
家蚕Bombyx mori不同品种对人工饲料的摄食性存在很大差异。为探讨食性差异的分子机理,本文基于对人工饲料摄食性不同的蚕品种(系)SAGE(serial analysis of gene expression)文库差异表达基因的分析,发掘了1条家蚕假定表皮蛋白(cuticula...家蚕Bombyx mori不同品种对人工饲料的摄食性存在很大差异。为探讨食性差异的分子机理,本文基于对人工饲料摄食性不同的蚕品种(系)SAGE(serial analysis of gene expression)文库差异表达基因的分析,发掘了1条家蚕假定表皮蛋白(cuticular protein hypothetical)基因BmCph2。采用半定量RT-PCR和实时荧光定量PCR方法,对BmCph2在不同摄食性蚕品种(系)不同发育时期的表达特征进行了研究。结果表明:BmCph2基因在家蚕幼虫眠期和起蚕期高表达,在胚胎期和幼虫将眠期几乎检测不到表达;在幼虫头部与全蚕的表达特征相似,而在中肠中表达活性很低,推测该基因表达可能与家蚕新表皮的形成有关。BmCph2在对人工饲料摄食性不同的蚕品种(系)中的表达存在较大差异,在摄食性好的高食性品种中表达量显著低于摄食性差的低食性品种;饲料和忌避剂的气味刺激及取食刺激对不同品种(系)该基因的表达有不同的影响,高食性蚕对诱导刺激比较敏感,而低食性蚕受影响较小,尤其是菁松A和菁松B的低食性品系几乎不受影响。本研究结果说明,BmCph2基因除可能参与表皮形成的同时,还与家蚕的食性有密切关系,但其具体机理有待于进一步研究。展开更多
文摘No or little information on the use fresh(wet)housefly maggots(Musca domestica)in African catfish(Clarias gariepinus)fry feeding.Therefore,this study was conducted to investigate the effect of feeding on fresh(wet)housefly maggots with or without artificial diet on water quality,growth performance,survival percentage and feed utilization of African catfish fry under laboratory conditions.Housefly maggots produced from a mixture of poultry droppings and foods wastes,it was used to replace artificial feed at 0,50 and 100% levels.Catfish were fed artificial diet alone(Feed 1),fresh(wet)housefly maggots alone(Feed 2),and 50% fresh housefly maggots with 50% artificial diet(Feed 3)were prepared and tested on triplicate groups of African catfish fry(initial weight of 0.25±0.02 g)for 60 days.Results showed that final weight(g/fish)was significantly(P≤0.05)higher in fish fed on feed 3(6.03±0.08),followed by fish fed feed 2(4.62±0.27),followed by fish fed feed 1(3.15±0.68).Specific growth rate(%/day)was also significantly higher in fish fed on feed 3(5.31±0.10),followed by fish fed feed 2(4.86±0.03),followed by fish fed feed 1(4.18±0.24).The same trend was observed with total weight gain,percentage weight gain,daily growth rate and relative growth rate.Feed intake and protein intake were significantly(P≤0.05)higher in fish fed on feed 3 and fish fed on feed 2,followed by fish fed feed 1.While,feed conversion ratio(FCR)and protein efficiency ratio were not significantly(P>0.05),but the improvement in FCR recorded in catfish fry fed feed 3 and feed 2 under the experimental conditions.Survival percentage was within the range 55-75%,with insignificant differences(P>0.05)among treatments.The water quality parameters such as temperature,pH,dissolved oxygen,total ammonia,nitrite and nitrate were not significantly(P>0.05)between the treatments and were tolerable for Catfish culture.Accordingly,use of the 50% fresh(wet)housefly maggots with 50% artificial diet in African catfish fry feeding had positive effect on growth performance and reduce of the feed cost.
文摘家蚕Bombyx mori不同品种对人工饲料的摄食性存在很大差异。为探讨食性差异的分子机理,本文基于对人工饲料摄食性不同的蚕品种(系)SAGE(serial analysis of gene expression)文库差异表达基因的分析,发掘了1条家蚕假定表皮蛋白(cuticular protein hypothetical)基因BmCph2。采用半定量RT-PCR和实时荧光定量PCR方法,对BmCph2在不同摄食性蚕品种(系)不同发育时期的表达特征进行了研究。结果表明:BmCph2基因在家蚕幼虫眠期和起蚕期高表达,在胚胎期和幼虫将眠期几乎检测不到表达;在幼虫头部与全蚕的表达特征相似,而在中肠中表达活性很低,推测该基因表达可能与家蚕新表皮的形成有关。BmCph2在对人工饲料摄食性不同的蚕品种(系)中的表达存在较大差异,在摄食性好的高食性品种中表达量显著低于摄食性差的低食性品种;饲料和忌避剂的气味刺激及取食刺激对不同品种(系)该基因的表达有不同的影响,高食性蚕对诱导刺激比较敏感,而低食性蚕受影响较小,尤其是菁松A和菁松B的低食性品系几乎不受影响。本研究结果说明,BmCph2基因除可能参与表皮形成的同时,还与家蚕的食性有密切关系,但其具体机理有待于进一步研究。
