Effects of Exogenous H_(2)O_(2) on ROS Metabolism in Nitraria tangutorum Bobr. Leaves Under Salt Stress

Salt stress is a major abiotic stress limiting plant growth and yield. In the present study, the effects of exogenous H_(2)O_(2) on the reactive oxygen species(ROS) metabolism and the antioxidant system in leaves of Nitralia tangutorum Bobr. under salt stress were studied. N. tangutorum seedlings were subjected to 200 mmol·L^(-1) NaCl treatment with or without the exogenous application of H_(2)O_(2) for 7 days. The results showed that NaCl stress significantly increased the relative conductivity, the contents of thiobarbituric acid reactive substances(TBARS) and ROS(H_(2)O_(2) and O_(2)^(·-)), as well as promoted the activities of antioxidant enzymes including superoxide dismutase(SOD), peroxidase(POD), catalase(CAT), and ascorbate peroxidase(APX) in N. tangutorum leaves. In addition, exogenous H_(2)O_(2) decreased the relative conductivity, the contents of TBARS, H_(2)O_(2) and O_(2)^(·-), while further enhanced the activities of antioxidant enzymes. These results indicated that H_(2)O_(2) effectively alleviated the adverse effects of NaCl stress on N. tangutorum through the regulation of ROS metabolism.

Identification of Tibetan and Qiang Edible and Medicinal Herbs Cardamine tangutorum and Cardamine macrophylla

[Objectives]To study the original plants,characters,tissue structure,powder characteristics and thin-layer chromatography(TLC)characteristics of Cardamine tangutorum and Cardamine macrophylla as Tibetan and Qiang edible and medicinal herbs,and to provide the basis for the identification of C.tangutorum and C.macrophylla.[Methods]The identification of C.macrophyll and C.tangutorum was carried out by original plant identification,character identification,microscopic identification and TLC identification.[Results]C.tangutorum and C.macrophylla can be distinguished according to the shape of rhizome and stem,the difference of stem leaves and leaflets,and the difference of flower color;there is no obvious difference between the characteristics of the shape and the powder;the thin layer chromatography shows that in the thin layer chromatography of C.tangutorum and C.macrophylla,spots with the same color are shown on the corresponding positions of the ground part and the reference substance quercetin;the underground part and the position corresponding to the reference substanceβ-sitosterol all show the same color spots.[Conclusions]This study provides a reference for the identification of C.tangutorum and C.macrophylla.

青海高原优势禾草-麦宾草内生真菌最佳培养条件筛选

本研究以青海高原5个生态型麦宾草(Elymus tangutorum)中分离的Epichloë内生真菌为研究对象,观测分离的5株麦宾草内生真菌菌株在不同碳源和氮源培养条件下菌落形态、分生孢子形态与大小、菌落生长速度等特性,进而确定各个菌株的最佳培养条件。结果表明:1)不同生态型麦宾草中分离的Epichloë内生真菌最适碳源和氮源存在差异。其中,1、3号菌株分别以蔗糖和胰蛋白胨为碳源和氮源时,Epichloë内生真菌的菌落生长速度最大,而2、4号菌株的菌落生长速度在以淀粉为碳源和蛋白胨为氮源时最高,5号菌株的最适碳源和氮源分别为麦芽糖和胰蛋白胨。2)不同碳源处理下,2、3、5号菌株菌落形态相似,正面中央白色凸起,具有生长圈,背面颜色由中央向边缘由土黄色过渡为淡黄色;1号菌株在淀粉条件下边缘菌丝呈不规则形状;4号菌株在果糖条件下生长缓慢。不同氮源处理下,5个菌株菌落正面中央白色凸起,背面颜色由中央至边缘逐渐变浅;在钼酸铵条件下,仅观察到白色小圆点;而在不添加任何氮源时,菌落边缘气生菌丝稀疏呈透明色。3)分生孢子形态多为椭圆形、圆形、肾形或两端不对称,在不同碳源和氮源处理下,同一菌株的分生孢子大小和分生孢子梗长度及基宽差异显著(P<0.05)。上述研究表明,不同生态型麦宾草−Epichloë内生真菌生长所需碳、氮源存在差异,筛选麦宾草内生真菌生长最佳培养碳氮源将为利用麦宾草内生真菌共生体进行披碱草属禾草新种质创制提供菌源。

盐碱土壤中唐古特白刺内生真菌分离与鉴定

为探明唐古特白刺(Nitraria tangutorum Bobr.)的内生真菌种属及其组织分布特性,采用传统组织块法从生长于盐碱土壤的白刺中分离内生真菌,运用形态学和真菌ITS序列分析进行种属鉴定,并通过MEGA7.0构建系统发育树。结果显示,从白刺(根、茎、叶)分离得到23株内生真菌,共鉴定出21株,其中链格孢属(Alternaria)13株,毛壳菌属(Chaetomium)1株,篮状菌属(Talaromyces)3株,细基格孢属(Ulocladium)4株,主要分布于唐古特白刺的根部,叶次之,茎则无。表明白刺内生真菌分布具有组织差异,根是其侵染和定殖的主要部位。

乌兰布和沙漠不同演替阶段白刺灌丛沙堆土壤养分和粒径的变化特征

为探究乌兰布和沙漠不同演替阶段下白刺灌丛沙堆土壤理化性质的变化特征。本研究按照白刺灌丛枯死率和盖度划分不同演替阶段,分别为发育阶段(枯死率10%~30%,盖度20%~40%)、稳定阶段(枯死率≤10%,盖度40%~60%)和衰退阶段(枯死率≥50%,盖度≤20%),每个演替阶段选取3个相对独立的白刺灌丛沙堆作为研究对象,研究土壤养分和土壤粒径的变化特征。结果表明:不同坡向白刺灌丛沙堆表层土壤养分含量随着沙堆演替呈下降趋势。背风坡发育阶段有机质、速效氮和速效磷含量分别较衰退阶段高164.58%,144.44%和215.93%,而迎风坡发育阶段有机质、速效氮和速效磷含量分别较衰退阶段高107.95%,200%和45.64%。演替阶段对迎风坡处白刺沙堆灌丛土壤养分含量和粒径组成产生显著影响(P<0.05)。综上所述,不同演替阶段白刺灌丛沙堆表层土壤养分富集现象明显,但不同坡向相同演替白刺灌丛沙堆土壤理化性质存在差异。

On Spatial Variability and Distribution of Soil Nutrients in Different Nitraria tangutorum Bobr.Populations in Tsaidam Basin

In this paper,six important distribution areas of Nitraria tangutorum Bobr.in Tsaidam Basin were selected as research objects,to study spatial variability and distribution of soil nutrients in different N.tangutorum populations and analyze the relationship between soil nutrient contents and geographical location,by measuring soil pH and the contents of organic matter（OM）,total nitrogen（N）,total phosphorus（P）,total potassium（K）,hydrolysis N,available P and available K in soils.Results showed that：（1） soil nutrient contents among different populations showed significant spatial variability,and soil depth had a significant effect on soil nutrients contents,but the variation rules were not obvious.（2）Average pH and average contents of OM,total N,total P,total K,hydrolysis N,available P and available K in soils with different depths（0-15,15-30,30-45 cm） varied in the range of 8.37-9.21,3.34-20.68,0.18-1.21,0.35-0.75,16.12-22.04,5.13-553.28,1.10-52.54 and 103.83-562.28 mg/kg,respectively.（3） The analysis results of correlation between average values of pH and contents of nutrient indexes in soils with different depths（0-15,15-30,30-45 cm） showed that the correlation of these indexes were different.（4）OM and total N contents in soils with different depths（0-15,15-30,30-45 cm） all had a significant positive correlation with latitude and negative correlation with longitude and altitude,and the correlation of available P and available K contents in surface soils（0-15 cm） with latitude,longitude and altitude were significant positive,significant negative and significant negative,respectively;moreover,longitude and latitude also showed a significant impact on soil available K contents with the depth of 30-45 cm.In addition,comprehensive analysis result of nutrient contents showed that N.tangutorum populations in Huaitou Tala Town had the highest fertility,and the fertility levels of N.tangutorum populations in Chaka Town and Wulan Keke Town were relatively lower.

白刺(Nitraria tangutorum)灌丛沙堆干沙层土壤水分动态及其对小雨事件的响应

干沙层对抑制蒸发、保护沙漠地区的生态水具有重要作用。以乌兰布和沙漠东缘白刺灌丛沙堆不同部位的干沙层为研究对象,采用烘干称重法对降雨前后灌丛沙堆不同部位干沙层土壤水分进行连续测定,结果表明:(1)从土壤水分空间变异来看,0~10cm干沙层土壤水分变化受降雨影响最为明显,4.3mm降雨能影响到0~5cm干沙层土壤水分,5.4mm和10.8mm降雨能影响到0~15cm干沙层土壤水分;(2)从土壤水分时间动态来看,与降雨前相比,降雨后干沙层土壤水分呈迅速增加-逐渐降低的变化趋势,在降雨后第1d增加最为明显,然后从降雨后第2d开始逐渐下降;(3)位于沙丘不同部位的干沙层土壤水分对降雨的响应存在显著差异,降雨后干沙层厚度变化速率表现为:阳坡中部>阳坡底部>阴坡底部>阴坡中部>坡顶。小降雨对白刺灌丛沙堆干沙层的消失和产生有重要影响,干沙层的形成是水分下渗和地表蒸发共同作用的结果。

麦宾草(Elymus tangutorum)栽培驯化研究初报

对当地野生草种麦宾草进行了驯化栽培的初步研究。结果表明:麦宾草在海拔高度3 800m左右的果洛地区在栽培条件下能完成整个生育期生长,栽培当年高度可达到16.0cm,盖度达到60%,第2年株高可达到110cm,盖度达到96%;栽培当年干草产量为1 266.8kg/hm2,第2年、第3年和第4年的干草产量分别为7 959.4kg/hm2,6 462.4kg/hm2,5 244.1 kg/hm2,种子产量分别为1 000.2kg/hm2,880.6kg/hm2,790.5kg/hm2。该草种是适合三江源区黑土滩退化草地植被恢复与重建的优良草种,也是高寒草甸地区建植高质量的生态型或刈用型黑土滩人工草地的优良草种。

乌兰布和沙漠不同演替阶段白刺灌丛沙堆土壤养分分布与富集特征

[目的]探究乌兰布和沙漠不同演替阶段白刺灌丛沙堆土壤养分垂直分布情况,为该区白刺灌丛稳定发展提供科学依据。[方法]选取不同演替阶段白刺灌丛沙堆作为研究对象。通过前期植被调查,依据白刺灌丛枯死率和盖度将其划分不同演替阶段,分别为发育阶段(枯死率10%~30%,盖度20%~40%)、稳定阶段(枯死率<10%,盖度40%~60%)和衰退阶段(枯死率>50%,盖度<20%),分别选取每个演替阶段3个相对独立的白刺灌丛沙堆,在丘间地、沙堆迎风坡和背风坡进行植被特征调查和土壤养分测定。[结果]随着白刺灌丛演替推进,白刺高度、冠幅长度和宽度呈先升后降趋势,其中,稳定阶段冠幅长度显著高于其他阶段(p<0.05),较发育阶段和衰退阶段分别增加45.50%和50.01%(p<0.05)。背风坡处,发育阶段土壤养分含量随着土层深度增加均呈“降—升—降”趋势,稳定和衰退阶段有机质含量呈下降趋势,40—60 cm达到最小值。迎风坡处,40—60 cm土层土壤有机质和全氮含量随着演替阶段发展在衰退阶段达到最大值,但发育和衰退阶段土壤全氮含量随着土层深度增加在40—60 cm达到最小值,较0—10 cm土层分别低83.33%和80.31%(p<0.05)。不同坡位白刺灌丛沙堆土壤养分均在0—10 cm达到最大值,但总体上白刺灌丛沙堆土壤养分富集作用不明显。[结论]白刺灌丛沙堆土壤养分主要集中在沙堆表层,如何缓解白刺灌丛沙堆活化和土壤养分流失,是白刺灌丛沙堆在干旱环境维持稳定的重要保障。

白刺叶提取物缓解溃疡性结肠炎及抗氧化作用评估

研究唐古特白刺叶对3%葡聚糖硫酸钠(DSS)诱导的溃疡性结肠炎小鼠的缓解作用及可能的作用机制.采用DSS诱导小鼠溃疡性结肠炎,用唐古特白刺叶提取物给予治疗,通过HE染色法、疾病活动指数(DAI)、结肠组织病理学评分、酶联免疫吸附测定法(ELISA)检测血清中炎性因子IL-6、IL-1β、TNF-α的水平,并采用HPLC-ESI-HRMS/MS对白刺叶提取物进行化学成分指认和鉴定,在化学成分的基础上,基于HepG-2细胞对活性氧(ROS)、谷胱甘肽转移酶(GSH)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)等抗氧化酶水平进行检测.与正常组比较,模型组结肠显著缩短,DAI评分显著增加,血清中IL-6、IL-1β、TNF-α水平(P<0.01)升高,在HepG-2细胞中,模型组活性氧(ROS)水平升高,GSH、SOD、CAT降低(P<0.05);与模型组比较,白刺叶提取物组小鼠DAI评分和血清中IL-6、IL-1β、TNF-α水平降低(P<0.01),溃疡性结肠炎症状减轻,且在HepG-2细胞中,白刺叶提取物组ROS水平降低,GSH、SOD、CAT水平升高(P<0.05).唐古特白刺叶对DSS诱导的溃疡性结肠炎具有治疗作用,其作用机制可能与降低炎症因子和活性氧水平,同时升高抗氧化酶密切相关.

民勤荒漠绿洲过渡带天然白刺群落多样性及其对降雨的响应

为了解降水对白刺灌丛生物多样性的影响,在民勤青土湖绿洲-荒漠过渡带流动沙丘和半固定沙丘上设立固定样地并进行生物多样性调查,结合年降水量,利用统计学原理和方法开展白刺灌丛群落对降雨的响应研究.结果表明:在流动沙丘和半固定沙丘中,白刺群落组成对降水的响应明显;在白刺群落中,1年生草本对降雨响应强烈,多年生草本和灌木半灌木对降雨响应不显著;群落中植物种数、物种植株数量对降雨响应强烈;白刺冠幅、当年生长量、盖度与降水量均成正相关关系.

不同白刺品种和不同播种季节对苗木生长的影响

为探明阿拉善地区白刺播种育苗最佳用种及播种季节,以大果白刺、小果白刺种子为材料进行冬季、春季分期播种育苗对比试验。结果表明:大果白刺春播苗出苗率较高,且苗高、地径、根数、地上生物量和地下生物量显著高于小果白刺春播苗,其各项生长指标比小果白刺分别高出36.22%、39.90%、80.89%、91.31%和121.59%;大果白刺冬播苗苗高显著高于春播苗。阿拉善地区在播种育苗时采用大果白刺进行冬季播种有利于提高出苗率和生长量,能够保证种苗质量。

Synergistic combination of arbuscular mycorrhizal fungi and plant growth-promoting rhizobacteria modulates morpho-physiological characteristics and soil structure in Nitraria tangutorum bobr.Under saline soil conditions

Nitraria tangutorum Bobr.,a typical xero-halophyte,can be used for vegetation restoration and reconstruction in arid and semiarid regions affected by salinity.However,global climate change and unreasonable human activity have exacerbated salinization in arid and semi-arid regions,which in turn has led to the growth inhibition of halophytes,including N.tangutorum.Arbuscular mycorrhizal fungi(AMF)and plant growth-promoting rhizobacteria(PGPR)have the potential to improve the salt tolerance of plants and their adaptation to saline soil environments.In this study,the effects of single and combined inoculations of AMF(Glomus mosseae)and PGPR(Bacillus amyloliquefaciens FZB42)on N.tangutorum were evaluated in severe saline soil conditions.The results indicate that AMF and PGPR alone may not adapt well to the real soil environment,and cannot ensure the effect of either growth promotion or salt-tolerance induction on N.tangutorum seedlings.However,the combination of AMF and PGPR significantly promoted mycorrhizal colonization,increased biomass accumulation,improved morphological development,enhanced photosynthetic performance,stomatal adjustment ability,and the exchange of water and gas.Co-inoculation also significantly counteracted the adverse effect of salinity on the soil structure of N.tangutorum seedlings.It is concluded that the effectiveness of microbial inoculation on the salt tolerance of N.tangutorum seedlings depends on the functional compatibility between plants and microorganisms as well as the specific combinations of AMF and PGPR.

Optimization of Ultrasonic-enzymatic Extraction of Total Flavonoids from Cardamine tangutorum O.E.Schulz by Response Surface Methodology and Content Determination

[Objectives]This study aimed to optimize the extraction process of total flavonoids in Cardamine tangutorum O.E.Schulz.[Methods]On the basis of single-factor experiments,the ultrasonic-enzymatic extraction process of total flavonoids in C.tangutorum O.E.Schulz was optimized by Box-Behnken design from aspects of ethanol volume fraction,extraction time,extraction temperature and enzyme addition,with yield of total flavonoids as the evaluation index.[Results]The optimal extraction process of total flavonoids in C.tangutorum O.E.Schulz was as follows:ethanol volume fraction of 70%,ultrasonic extraction time of 43 min,ultrasonic extraction temperature of 37℃and cellulose addition of 0.07 mg.Under the optimal conditions,the yield of total flavonoids from C.tangutorum O.E.Schulz reached 75.90 mg/g.[Conclusions]The optimized extraction process was reasonable and feasible,and it could provide a reference for the extraction of total flavonoids in C.tangutorum O.E.Schulz.This study provided a foundation for the quality control of food/medicinal materials and the development and research of big health products of C.tangutorum O.E.Schulz.

西藏麦宾草(Elymus tangutorum)种子特征研究

种子特征与幼苗的生存、发育和植株未来的竞争密切相关。本研究对分布在林芝地区八一镇野生草种西藏麦宾草(Elymus tangutorum)种子为试验对象,通过观测麦宾草主要形态特征,并对其种子特征及其萌发特性进行了分析。结果表明:西藏麦宾草的主杆长度、穗长、小穗数量的平均值分别为1354.65 mm、165.70 mm和51.85个,麦宾草种子扁平,圆形或半圆形,种子芒长介于1.0 mm^11.0 mm之间,平均值为8.0 mm,种子长度介于7.0 mm^14.0 mm之间,平均值为9.6 mm。通过对3个不同培养皿麦宾草种子千粒重、含水率及发芽率进行研究,发现西藏麦宾草种子千粒重(4.705 g、4.776 g、4.542 g)、含水率(11.65%,12.34%,10.49%)和发芽率(73.70%,78.89%,59.63%)的平均值分别为4.67g、11.49%、70.74%,根(12.33 mm/d,12.35 mm/d,10.16 mm/d)和苗(17.67 mm/d,16.79 mm/d,13.33 mm/d)的生长速率平均值分别为11.61 mm/d、15.93 mm/d。且均表现为2号培养皿>1号培养皿>3号培养皿。总之,麦宾草种子千粒重越大,相应的含水率、发芽率也相对较高且生命力旺盛,萌发之后根和苗的生长速率也相对较快,发育过程中生长势呈现良好状态。

Determination and Multidimensional Analysis of 29 Inorganic Elements in Cardamine tangutorum O.E.Schulz of Different Origins

[Objectives]This study aimed to determine the contents of inorganic elements in Cardamine tangutorum O.E.Schulz samples from different origins to provide a scientific reference for the quality control,safety evaluation and clinical medicinal use of C.tangutorum O.E.Schulz.[Methods]The contents of Al,As,B,Ba,Ca,Cd,Co,Cr,Cu,Fe,Hg,K,Mg,Mn,Mo,Na,Ni,P,Pb,Pd,Rb,Se,Si,Sn,Sr,Ti,Tl,V and Zn in the C.tangutorum O.E.Schulz samples were determined simultaneously by wet digestion and inductively coupled plasma optical emission spectroscopy(ICP-OES),and the determination results were analyzed by principal component analysis.[Results]A total of 28 inorganic elements were detected in the samples of C.tangutorum O.E.Schulz from different origins.The contents of heavy metals did not exceed the limits.Among the elements,the determined values of Hg in all samples were below the detection limit,so it was not detected.The contents of Ca,Al,P and Fe were relatively high,and they were essential nutrients in the human body.There were significant differences among different elements.Samples from different origins show similar patterns in the distribution of main elements.Correlation analysis shows that 15 pairs of inorganic elements out of 24 elements had extremely significant positive correlations,and 26 pairs of elements had significant positive correlations.The principal component analysis determined Al,As,Ba,Ca,Cd,Fe,Mn,Ni,P,Pb,Pd,Se,V and Zn were the characteristic inorganic elements in the samples of C.tangutorum O.E.Schulz.[Conclusions]The contents of toxic heavy metals in C.tangutorum O.E.Schulz samples from different origins met the edible requirements.The method was simple,rapid and accurate,and could be used to analyze and determine the inorganic elements in the samples of C.tangutorum O.E.Schulz,providing a theoretical basis for the better development and utilization of C.tangutorum O.E.Schulz.

青海柴达木盆地唐古特白刺果实酿酒潜力分析

为综合开发唐古特白刺果实生物资源,以青海省5个天然分布的唐古特白刺居群为研究对象,比较分析其果实中的基本理化指标(还原糖、总酸、pH)、酚类物质(单宁、总酚、花色苷)、香气物质等含量,为进一步开发利用唐古特白刺果实提供数据支撑。结果表明:1)果实还原糖含量达154.67~291 g·L^(-1),适合进行酒精发酵类饮品加工;2)果实总酚含量达10.18~13.41 mg·g^(-1),花色苷含量达1.31~1.59 mg·g^(-1),适合酿造干红类果酒;3)果实中共有52种挥发性物质,总量最高达5776.801μg·L^(-1),芳香浓郁,可酿造具有特征芳香的干型果酒。因此,唐古特白刺果实在干型果酒酿造方面具有较大的开发潜力。

草地白刺粗角叶甲成虫触角感器的超微形态学

白刺粗角叶甲(Diorhabda rybakowi)是严重危害我国西北荒漠草地防风固沙先锋植物唐古特白刺(Nitraria tangutorum)和泡泡刺(N.sphaerocarpa)的主要害虫。利用扫描电子显微镜技术对该虫的触角超微结构进行研究,并比较雌雄种间的差异。结果表明,白刺粗角叶甲雌、雄成虫触角均为丝状,鞭节分为9个亚节,且雄虫触角(4174.5μm)显著长于雌虫(3780.5μm)(P=0.006)。雌、雄虫触角表面均分布有大量表皮孔和鱼鳞状感觉附肢。雌、雄虫共鉴定到6种感器,分为刺形感器、毛形感器、锥形感器、腔锥形感器、耳形感器和Böhm氏鬃毛;其中,腔锥形感器仅在雄虫触角梗节少量分布。这为研究白刺粗角叶甲感知寄主挥发物或性信息素的嗅觉分子机制进而为其综合防控奠定了基础。

干旱荒漠区白刺灌丛沙堆土壤温度及水分养分变化特征

以民勤沙区不同变化阶段白刺灌丛沙堆为研究对象,测定白刺灌丛沙堆高度、长短轴、迎风坡与背风坡植被株高、盖度、枯枝和结实等形态特征,分析不同变化阶段土壤温度及水分养分变化特征。结果表明,1)土层0~10 cm处的土壤温度随着时间推移地面温度呈现出先逐渐升高而后缓慢下降的趋势,日变化规律为发育阶段>雏形阶段>稳定阶段>衰退阶段。2)在不同发育阶段土壤含水率在40~60 cm土层中最高,0~5 cm最低,随着土层深度增加水分含量呈现逐渐升高的趋势。3)不同变化阶段土层pH在5~10 cm最高,不同坡向pH均为迎风坡大于背风坡;衰退阶段背风坡中电导率最高,均大于其他发育阶段的坡向。4)土壤TN、速效磷平均含量发育阶段最高、有机质衰退阶段最高、稳定阶段均最低;土壤TN为雏形阶段、发育阶段和衰退阶段迎风坡中大于背风坡中,稳定阶段则相反;土壤有机质为雏形阶段和发育阶段背风坡中大于迎风坡中,稳定阶段和衰退阶段则相反;土壤速效磷为不同发育阶段迎风坡中大于背风坡中,土壤温度在0~10 cm处日动态呈现出先逐渐升高而后缓慢下降的趋势;土壤含水率在40~60 cm土层中最高,0~5 cm最低;pH在5~10 cm最高;电导率在衰退阶段背风坡中最高;土壤TN、有机质和速效磷在发育阶段表面养分含量达到最高,且显著高于其他几个阶段。研究结果为民勤荒漠区白刺群落植被恢复与保护提供科学理论依据。

干旱胁迫和不同株高基径条件下的白刺液流速率特征差异

[目的]揭示内蒙古荒漠环境中不同株高基径的白刺液流差异,阐明不同形态白刺对干旱环境的适应情况,为干旱区荒漠绿洲植被恢复的白刺种植提供参考。[方法]在乌兰布和沙漠选择高株高组(HS)和粗基径组(BD)的典型白刺,并模拟防止水分横向流失的隔水处理条件(CG),采用野外定点观测的方法监测和分析白刺生长季液流速率变化情况及其与环境因子的关系。[结果](1)白刺液流速率在月尺度上的大小顺序为8月>7月>6月>9月>5月,隔水组(WS)白刺液流速率较正常组(NW)变化幅度大,液流速率中值分别为1.021 g/h,0.387 g/h;基径大植株矮组(LS)白刺液流速率较基径小植株高组(SH)变化幅度大,液流速率中值分别为0.472 g/h,0.261 g/h。(2)在土壤水分长期匮缺的条件下,液流速率与气温(Ta)、风速(WVEL)呈显著正相关,与光合有效辐射(PAR)呈显著负相关,与空气湿度(RH)相关性不明显,环境因子对液流速率影响程度依次为:PAR>Ta>VPD>WVEL>RH;30—150 cm深度土层土壤含水率对白刺液流速率均呈显著的正相关关系。(3)白刺液流速率与环境因子的回归方程表明,LS组较SH组液流速率受气象因子的影响更大,WS组白刺液流速率仅受30—50 cm土层土壤含水率的影响。[结论]大基径矮植株白刺较小基径高植株的白刺更能适应当地干旱的气候条件,粗矮形态的白刺更适应在缺水环境下生存。