Kalimeris integrifolia Turcz. is a root sucker form perennial herb of Compositae. In die late period of growing season, fresh fleshy radicels grow up above the soil surface to develop root-borne buds, which in turn gr...Kalimeris integrifolia Turcz. is a root sucker form perennial herb of Compositae. In die late period of growing season, fresh fleshy radicels grow up above the soil surface to develop root-borne buds, which in turn grow into ramets and taproot. On the basis of observation on numerous samples, methods to classify the age of ramets and buds for the root sucker herbaceous plant population and to estimate the ages of taproots were established according to their morphology. The age of ramets was classified based on the regeneration times of taproot buds. Ramets developed from new root sucker buds were classified as 1 a. After 1 a ramets died, the ramets reproduced from the taproots were 2 a, and so on. New buds from a root sucker were 0 a buds, other age classes of buds were consistent with that of ramets, i.e. buds produced from taproots of I a ramets were 1 a buds, and so on. In the hay clipping meadows of the Songnen Plains, for K. integrifolia population, there are three age classes of ramets, representing a steady age structure. The maximal age of taproots of ramets is 4 a. Among all age classes, the population productivity of 2 a ramets was the highest, and that of I a ramets was the lowest. The ramet productivity increased with ramet age. During the early period of growing season, the productivity of 3 a ramets was the highest, but after the middle of growing season, I a ramets showed the greatest relative growth rate. The population size and age structure in the next year could be accurately forecasted by the number of fresh roots with budlet and present population in late autumn. The potential population in the bud banks was more than two times of the present population, which may provide sufficient substitution in the future.展开更多
目的:建立白芍药材主根与根茎的高效液相色谱(HPLC)指纹图谱,并比较两者的异同。方法:色谱柱为Phenomenex C18,流动相为乙腈-0.1%磷酸溶液(梯度洗脱),流速为1.0 m L/min,检测波长为230 nm,柱温为30℃,进样量为10μL。以芍药苷为参照,测...目的:建立白芍药材主根与根茎的高效液相色谱(HPLC)指纹图谱,并比较两者的异同。方法:色谱柱为Phenomenex C18,流动相为乙腈-0.1%磷酸溶液(梯度洗脱),流速为1.0 m L/min,检测波长为230 nm,柱温为30℃,进样量为10μL。以芍药苷为参照,测定白芍药材主根与根茎部分的HPLC图谱,采用《中药色谱指纹图谱相似度评价系统》(2012版)进行共有峰指认和相似度评价。结果:白芍药材主根与根茎的HPLC图谱有9个共有峰;白芍药材主根与根茎之间相似度>0.9。结论:该研究所建指纹图谱可为白芍药材的鉴别和质量评价提供参考,白芍药材主根与根茎有效成分一致,差异性较小。展开更多
文摘Kalimeris integrifolia Turcz. is a root sucker form perennial herb of Compositae. In die late period of growing season, fresh fleshy radicels grow up above the soil surface to develop root-borne buds, which in turn grow into ramets and taproot. On the basis of observation on numerous samples, methods to classify the age of ramets and buds for the root sucker herbaceous plant population and to estimate the ages of taproots were established according to their morphology. The age of ramets was classified based on the regeneration times of taproot buds. Ramets developed from new root sucker buds were classified as 1 a. After 1 a ramets died, the ramets reproduced from the taproots were 2 a, and so on. New buds from a root sucker were 0 a buds, other age classes of buds were consistent with that of ramets, i.e. buds produced from taproots of I a ramets were 1 a buds, and so on. In the hay clipping meadows of the Songnen Plains, for K. integrifolia population, there are three age classes of ramets, representing a steady age structure. The maximal age of taproots of ramets is 4 a. Among all age classes, the population productivity of 2 a ramets was the highest, and that of I a ramets was the lowest. The ramet productivity increased with ramet age. During the early period of growing season, the productivity of 3 a ramets was the highest, but after the middle of growing season, I a ramets showed the greatest relative growth rate. The population size and age structure in the next year could be accurately forecasted by the number of fresh roots with budlet and present population in late autumn. The potential population in the bud banks was more than two times of the present population, which may provide sufficient substitution in the future.
文摘目的:建立白芍药材主根与根茎的高效液相色谱(HPLC)指纹图谱,并比较两者的异同。方法:色谱柱为Phenomenex C18,流动相为乙腈-0.1%磷酸溶液(梯度洗脱),流速为1.0 m L/min,检测波长为230 nm,柱温为30℃,进样量为10μL。以芍药苷为参照,测定白芍药材主根与根茎部分的HPLC图谱,采用《中药色谱指纹图谱相似度评价系统》(2012版)进行共有峰指认和相似度评价。结果:白芍药材主根与根茎的HPLC图谱有9个共有峰;白芍药材主根与根茎之间相似度>0.9。结论:该研究所建指纹图谱可为白芍药材的鉴别和质量评价提供参考,白芍药材主根与根茎有效成分一致,差异性较小。