To mitigate the wastage of seed resources and reduce the usage of pesticides and fertilizers, seed coating agentshave gained popularity. This study employs single-factor and multi-index orthogonal experimental design ...To mitigate the wastage of seed resources and reduce the usage of pesticides and fertilizers, seed coating agentshave gained popularity. This study employs single-factor and multi-index orthogonal experimental design methodsto investigate the seed coating formula and physical properties of Tartary buckwheat. The specific effects ofeach component on Tartary buckwheat seed germination are analyzed. The findings reveal that the seed coatingagent formulated with 1.5% polyvinyl alcohol, 0.15% sodium alginate, 0.2% op-10, 0.1% polyacrylamide, 8% colorant,3% ammonium sulfate, 1% potassium dihydrogen phosphate, and 0.15% carbendazim exhibits the mosteffective coating. It demonstrates optimal physical properties and promotes seed germination efficiently. The suspensionrate of this seed coating agent reaches 91.12%, with a mere 2.13% coating shedding rate and 2.5% coatingseed rot rate. Furthermore, it achieves a germination percentage of 99.17%, which is 20.84% higher than the lowestgroup. The germination potential and index are also significantly higher than the lowest group, with anincrease of 20.84% and 26.56%, respectively. Additionally, the vitality index is 553.08, a 15.75% increase comparedto the lowest group. The application of seed coating agents helps reduce seed resource loss, increase plant numbers,and ultimately enhance agricultural yields. This finding holds practical significance in agriculturalproduction.展开更多
In order to study the changes and mechanism of phenolic compounds during Tartary buckwheat germination,the dynamic changes of phenolic compounds were analyzed,and the activities of enzymes that regulate phenolic compo...In order to study the changes and mechanism of phenolic compounds during Tartary buckwheat germination,the dynamic changes of phenolic compounds were analyzed,and the activities of enzymes that regulate phenolic compound biosynthesis and degradation were monitored.Total phenolics and total flavonoids presented an interesting dynamic trend with the extension of the germination time,and rutin,gallic acid,chlorogenic acid as well as 2,3,4-trihydroxybenzoic acid contents showed an increasing trend during germination period,the contents were up to(2663.4±61.1),(449.12±5.26),(99.953±7.800)and(50.442±1.477)mg/100 g DW,respectively.Phenylalanine ammonia lyase(PAL)and chalcone isomerase(CHI)are dominant enzymes in regulating phenolic compound biosynthesis,which showed an increasing trend.Rutin degrading enzyme(RDEs)is the key enzyme in regulating phenolic compound degradation,which showed a decreasing trend.These results suggested that germination processing increases the phenolic compound contents of Tartary buckwheat,which may be regulated by the activation of PAL,CHI and the inhibition of RDEs.展开更多
基金the Sichuan Science and Technology Program(2023NSFSC0214)China Agriculture Research System(CARS-07-B-1)+1 种基金National Natural Sciences Foundation of China(Nos.3230185031771716).
文摘To mitigate the wastage of seed resources and reduce the usage of pesticides and fertilizers, seed coating agentshave gained popularity. This study employs single-factor and multi-index orthogonal experimental design methodsto investigate the seed coating formula and physical properties of Tartary buckwheat. The specific effects ofeach component on Tartary buckwheat seed germination are analyzed. The findings reveal that the seed coatingagent formulated with 1.5% polyvinyl alcohol, 0.15% sodium alginate, 0.2% op-10, 0.1% polyacrylamide, 8% colorant,3% ammonium sulfate, 1% potassium dihydrogen phosphate, and 0.15% carbendazim exhibits the mosteffective coating. It demonstrates optimal physical properties and promotes seed germination efficiently. The suspensionrate of this seed coating agent reaches 91.12%, with a mere 2.13% coating shedding rate and 2.5% coatingseed rot rate. Furthermore, it achieves a germination percentage of 99.17%, which is 20.84% higher than the lowestgroup. The germination potential and index are also significantly higher than the lowest group, with anincrease of 20.84% and 26.56%, respectively. Additionally, the vitality index is 553.08, a 15.75% increase comparedto the lowest group. The application of seed coating agents helps reduce seed resource loss, increase plant numbers,and ultimately enhance agricultural yields. This finding holds practical significance in agriculturalproduction.
基金This research was funded by Guizhou Science and Technology Program(Qian Ke He Jichu[2019]1071,Qian Ke He Zhicheng[2020]1Y171Qian Ke He Zhicheng[2022]zhongdian007)+3 种基金Introducing Talents Program in Guizhou University(Gui Da Ren Ji He Zi(2017)46)Establishment of Guizhou Modern Agriculture Industry Technical System(Characteristic Coarse Cereals)(Qian Cai Nong[2018]81)Guizhou Key Agricultural Techniques Promotion Program(Qian Cai Nong[2017]106)The authors thanks Chun Mao,for providing the Tartary buckwheat materials.
文摘In order to study the changes and mechanism of phenolic compounds during Tartary buckwheat germination,the dynamic changes of phenolic compounds were analyzed,and the activities of enzymes that regulate phenolic compound biosynthesis and degradation were monitored.Total phenolics and total flavonoids presented an interesting dynamic trend with the extension of the germination time,and rutin,gallic acid,chlorogenic acid as well as 2,3,4-trihydroxybenzoic acid contents showed an increasing trend during germination period,the contents were up to(2663.4±61.1),(449.12±5.26),(99.953±7.800)and(50.442±1.477)mg/100 g DW,respectively.Phenylalanine ammonia lyase(PAL)and chalcone isomerase(CHI)are dominant enzymes in regulating phenolic compound biosynthesis,which showed an increasing trend.Rutin degrading enzyme(RDEs)is the key enzyme in regulating phenolic compound degradation,which showed a decreasing trend.These results suggested that germination processing increases the phenolic compound contents of Tartary buckwheat,which may be regulated by the activation of PAL,CHI and the inhibition of RDEs.
