Two new flavone glycosides, isoetin-7-O-β-D-glucopyranosyl-2′-O-α-L-arabinopyranoside （1） and isoetin-7-O-β-D-glucopyranosyl-2′-O-α-D-glucopyranoside （2）, a new lignan, mongolicumin A （3）, and a new guaian...Two new flavone glycosides, isoetin-7-O-β-D-glucopyranosyl-2′-O-α-L-arabinopyranoside （1） and isoetin-7-O-β-D-glucopyranosyl-2′-O-α-D-glucopyranoside （2）, a new lignan, mongolicumin A （3）, and a new guaianolide, mongolicumin B （4） were isolated from the aerial part of Taraxacum mongolicum. Their structures were elucidated mainly by spectral analyses.展开更多
Plants belonging to the genus Taraxacum are widespread all over the world,which contain rubber-producing and non-rubberproducing species.However,the genomic basis underlying natural rubber(NR)biosynthesis still needs ...Plants belonging to the genus Taraxacum are widespread all over the world,which contain rubber-producing and non-rubberproducing species.However,the genomic basis underlying natural rubber(NR)biosynthesis still needs more investigation.Here,we presented high-quality genome assemblies of rubber-producing T.kok-saghyz TK1151 and non-rubber-producing T.mongolicum TM5.Comparative analyses uncovered a large number of genetic variations,including inversions,translocations,presence/absence variations,as well as considerable protein divergences between the two species.Two gene duplication events were found in these two Taraxacum species,including one common ancestral whole-genome triplication and one subsequent round of gene amplification.In genomes of both TK1151 and TM5,we identified the genes encoding for each step in the NR biosynthesis pathway and found that the SRPP and CPT gene families have experienced a more obvious expansion in TK1151 compared to TM5.This study will have large-ranging implications for the mechanism of NR biosynthesis and genetic improvement of NR-producing crops.展开更多
研究旨在运用高效液相色谱法(HPLC)建立饲用植物蒲公英水提物的特征图谱,并用聚类分析和主成分分析对蒲公英水提物进行质量评价。采用Sunfire C18色谱柱,以乙腈-0.1%磷酸水为流动相,流速为0.8 m L/min,进样量10μL,柱温35℃,检测波长329...研究旨在运用高效液相色谱法(HPLC)建立饲用植物蒲公英水提物的特征图谱,并用聚类分析和主成分分析对蒲公英水提物进行质量评价。采用Sunfire C18色谱柱,以乙腈-0.1%磷酸水为流动相,流速为0.8 m L/min,进样量10μL,柱温35℃,检测波长329 nm,将22批蒲公英水提物色图谱导入中药色谱指纹图谱相似度评价系统软件(2012年版)中,选取分离度、峰型较好的8个峰为特征峰,得到蒲公英水提物特征图谱,并进行相似度评价、聚类分析和主成分分析。结果表明:试验建立的蒲公英水提物HPLC特征图谱共确定了8个特征峰,指认了单咖啡酰酒石酸、绿原酸、咖啡酸和菊苣酸4个色谱峰,相似度结果为0.869~0.999,说明22批蒲公英水提物质量相对稳定;当欧氏距离为4时,聚类分析将22批不同产地的蒲公英分成3类,S6一类,S4一类,其余批次为一类;主成分分析中单咖啡酰酒石酸和菊苣酸具有最大的贡献度。通过相似度评价、聚类分析和主成分分析与特征图谱相结合,可为蒲公英水提物质量评价提供参考。展开更多
文摘Two new flavone glycosides, isoetin-7-O-β-D-glucopyranosyl-2′-O-α-L-arabinopyranoside （1） and isoetin-7-O-β-D-glucopyranosyl-2′-O-α-D-glucopyranoside （2）, a new lignan, mongolicumin A （3）, and a new guaianolide, mongolicumin B （4） were isolated from the aerial part of Taraxacum mongolicum. Their structures were elucidated mainly by spectral analyses.
基金supported by the Strategic Priority Research Program of Chinese Academy of Sciences(XDA24030504 and XDA24040305)the National Natural Science Foundation of China(32000144)the Key Research Program of the Chinese Academy of Sciences(ZDRW-ZS-2018-2)。
文摘Plants belonging to the genus Taraxacum are widespread all over the world,which contain rubber-producing and non-rubberproducing species.However,the genomic basis underlying natural rubber(NR)biosynthesis still needs more investigation.Here,we presented high-quality genome assemblies of rubber-producing T.kok-saghyz TK1151 and non-rubber-producing T.mongolicum TM5.Comparative analyses uncovered a large number of genetic variations,including inversions,translocations,presence/absence variations,as well as considerable protein divergences between the two species.Two gene duplication events were found in these two Taraxacum species,including one common ancestral whole-genome triplication and one subsequent round of gene amplification.In genomes of both TK1151 and TM5,we identified the genes encoding for each step in the NR biosynthesis pathway and found that the SRPP and CPT gene families have experienced a more obvious expansion in TK1151 compared to TM5.This study will have large-ranging implications for the mechanism of NR biosynthesis and genetic improvement of NR-producing crops.
文摘研究旨在运用高效液相色谱法(HPLC)建立饲用植物蒲公英水提物的特征图谱,并用聚类分析和主成分分析对蒲公英水提物进行质量评价。采用Sunfire C18色谱柱,以乙腈-0.1%磷酸水为流动相,流速为0.8 m L/min,进样量10μL,柱温35℃,检测波长329 nm,将22批蒲公英水提物色图谱导入中药色谱指纹图谱相似度评价系统软件(2012年版)中,选取分离度、峰型较好的8个峰为特征峰,得到蒲公英水提物特征图谱,并进行相似度评价、聚类分析和主成分分析。结果表明:试验建立的蒲公英水提物HPLC特征图谱共确定了8个特征峰,指认了单咖啡酰酒石酸、绿原酸、咖啡酸和菊苣酸4个色谱峰,相似度结果为0.869~0.999,说明22批蒲公英水提物质量相对稳定;当欧氏距离为4时,聚类分析将22批不同产地的蒲公英分成3类,S6一类,S4一类,其余批次为一类;主成分分析中单咖啡酰酒石酸和菊苣酸具有最大的贡献度。通过相似度评价、聚类分析和主成分分析与特征图谱相结合,可为蒲公英水提物质量评价提供参考。
