In this study,the oil recovery from pumpkin seed with or without the presence of pigment was evaluated during the aqueous enzymatic extraction process(AEEP).Additionally,the interaction of pumpkin seed coat pigment-pr...In this study,the oil recovery from pumpkin seed with or without the presence of pigment was evaluated during the aqueous enzymatic extraction process(AEEP).Additionally,the interaction of pumpkin seed coat pigment-protochlorophyll(Pchl)with interfacial proteins(globulin-O/W,globulin-W,and albumin-W)during the aqueous extraction process was investigated.When Pchl was removed from pumpkin seeds,the yield of free oil was significantly increased by 50.71%after the first centrifugation.And the total oil recovery increased from 68.59%to 89.91%using enzymatic demulsification.Theβ-sheet percentage of globulin-O/W decreased by 5.6%with the presence of Pchl based on the circular dichroism spectra analysis.The particle size of globulin-W and albumin-W significantly increased in contrast to globulin-O/W.In addition,the increase of surface hydrophobicity and surface sulfhydryl group showed a conformational change of three protein extracts.Fluorescence spectra analyses showed that Pchl quenched the intrinsic fluorescence of globulin-W,albumin-W,and globulin-O/W in a static mode.Thermodynamic analyses indicated that the main force between Pchl and proteins was hydrophobic interactions.The hydrolysis efficiency of Papain was reduced during demulsification in AEEP with the presence of Pchl.Therefore,the removal of pumpkin seed coat pigments facilitated the aqueous extraction of seed oil by getting rid of the interaction of Pchl with its interfacial protein.展开更多
An aqueous enzymatic extraction method was used to extract oil from oil-tea camellia seed(Camellia oleifera).The factors influencing the yield rate such as enzyme kind,addition methods,concentration of enzyme,enzymati...An aqueous enzymatic extraction method was used to extract oil from oil-tea camellia seed(Camellia oleifera).The factors influencing the yield rate such as enzyme kind,addition methods,concentration of enzyme,enzymatic hydrolysis time and temperature,and ration of solid to liquid were investigated.The results are as following: The acid protease and cellulase have higher oil yield rate than the other treatments,but there is no significant difference between acid protease and cellulase(P<0.05).By directly adding acid protease to seed slurry of oil-tea camellia seed,more oil yield rate can be gotten than those treatments which added acid protease to ground slurry or filtered ground slurry.By single factor experiment,the optimum conditions of enzymolysis are: the concentration of acid protease,0.1 %(w/w);the enzymolysis temperature and time,55 ℃ and 3 h,respectively;and the ratio of solid to liquid,1∶10.展开更多
该文采用碱法和酶法两步加工碎米,制备得到纯度较高的大米蛋白。通过研究米粉粒度、pH值、温度、水料比和时间对提取率的影响,确定碱法制备大米蛋白的较佳工艺条件为:米粉粒度80目,pH 11.0,温度50℃,水料比8,时间120 m in。采用α-淀粉...该文采用碱法和酶法两步加工碎米,制备得到纯度较高的大米蛋白。通过研究米粉粒度、pH值、温度、水料比和时间对提取率的影响,确定碱法制备大米蛋白的较佳工艺条件为:米粉粒度80目,pH 11.0,温度50℃,水料比8,时间120 m in。采用α-淀粉酶对大米蛋白进行纯化,酶解条件为:加酶量60 U/g,pH 6.0,温度50℃,时间30 m in。在上述工艺条件下制备大米蛋白,提取率为73.22%,纯度为88.75%。展开更多
基金This research was financially supported by National Key R&D Program Project(2021YFD2100301)Key R&D Program Project of Xinjiang Autonomous Region(2022A02009).
文摘In this study,the oil recovery from pumpkin seed with or without the presence of pigment was evaluated during the aqueous enzymatic extraction process(AEEP).Additionally,the interaction of pumpkin seed coat pigment-protochlorophyll(Pchl)with interfacial proteins(globulin-O/W,globulin-W,and albumin-W)during the aqueous extraction process was investigated.When Pchl was removed from pumpkin seeds,the yield of free oil was significantly increased by 50.71%after the first centrifugation.And the total oil recovery increased from 68.59%to 89.91%using enzymatic demulsification.Theβ-sheet percentage of globulin-O/W decreased by 5.6%with the presence of Pchl based on the circular dichroism spectra analysis.The particle size of globulin-W and albumin-W significantly increased in contrast to globulin-O/W.In addition,the increase of surface hydrophobicity and surface sulfhydryl group showed a conformational change of three protein extracts.Fluorescence spectra analyses showed that Pchl quenched the intrinsic fluorescence of globulin-W,albumin-W,and globulin-O/W in a static mode.Thermodynamic analyses indicated that the main force between Pchl and proteins was hydrophobic interactions.The hydrolysis efficiency of Papain was reduced during demulsification in AEEP with the presence of Pchl.Therefore,the removal of pumpkin seed coat pigments facilitated the aqueous extraction of seed oil by getting rid of the interaction of Pchl with its interfacial protein.
文摘An aqueous enzymatic extraction method was used to extract oil from oil-tea camellia seed(Camellia oleifera).The factors influencing the yield rate such as enzyme kind,addition methods,concentration of enzyme,enzymatic hydrolysis time and temperature,and ration of solid to liquid were investigated.The results are as following: The acid protease and cellulase have higher oil yield rate than the other treatments,but there is no significant difference between acid protease and cellulase(P<0.05).By directly adding acid protease to seed slurry of oil-tea camellia seed,more oil yield rate can be gotten than those treatments which added acid protease to ground slurry or filtered ground slurry.By single factor experiment,the optimum conditions of enzymolysis are: the concentration of acid protease,0.1 %(w/w);the enzymolysis temperature and time,55 ℃ and 3 h,respectively;and the ratio of solid to liquid,1∶10.
文摘该文采用碱法和酶法两步加工碎米,制备得到纯度较高的大米蛋白。通过研究米粉粒度、pH值、温度、水料比和时间对提取率的影响,确定碱法制备大米蛋白的较佳工艺条件为:米粉粒度80目,pH 11.0,温度50℃,水料比8,时间120 m in。采用α-淀粉酶对大米蛋白进行纯化,酶解条件为:加酶量60 U/g,pH 6.0,温度50℃,时间30 m in。在上述工艺条件下制备大米蛋白,提取率为73.22%,纯度为88.75%。
