Effects of Tannic Acid on Active Aluminum Species Distribution in Various Tea Soils

Effects of tannic acid on active aluminum species distribution in different pH tea soils in Yunnan and Jiangxi tea gardens were studied by field observation and laboratory analysis.There were 4 species of active aluminum in the tea soil,i.e.,exchangeable aluminum Al3 +,unimer hydroxyl aluminum Al(OH)2 +Al(OH)+2,acid-soluble aluminum Al(OH)03and humic-acid aluminum Al-HA.Experimental results showed that tannic acid at levels 0 ~0.4 mmol/kg and 0 ~ 2.0 mmol/kg,with the rising of soil pH value,the content of exchangeable aluminum decreased significantly,hydroxyl aluminum,acid-soluble aluminum and humic-acid aluminum were increased gently.When the concentration of tannic acid increased to above 2.0 mmol/kg,the inhibition of higher concentration of tannic acid on content of active aluminum was strengthened with the rising of soil pH value and variation law of 20 ~ 40 cm soil layer was similar to 0 ~ 20cm soil layer.Generally speaking,the content of active aluminum in upper layer of soil were more than subsoil and total content of active aluminum in Yunnan tea soils were higher than Jiangxi tea soils.The correlation analysis showed that the soil pH were positively correlated with hydroxyl aluminum,humic-acid aluminum,pHBC(r = 0.796,0.960,0.852;p < 0.01,0.01,0.01) in 0 ~ 20cm soil layer.pHBC were significant negative correlated with exchangeable aluminum(r =- 904,p < 0.01),hydroxyl aluminum(r =- 645,p < 0.05),and with humic-acid aluminum have a positive correlation,correlation coefficient was0.795(P < 0.05).Meanwhile,the tannic acid addition concentration were 0 ~ 0.4 mmol/kg soils pH were increased significantly,while samples attains a peak pH value afterwards decrease continuously,the relationship between the pH and the concentration of tannic acids accords with the equation:Y pH=- 0.04C DN+3.82(R2= 0.95,P < 0.01).When the concentration of tannic acid up to about 8.0 ~ 12.0 mmol/kg,soil pH keep unchanged.Therefore,we can clearly draw that effects of tannic acid on active aluminum content of the inflection point is not the same in different region of tea garden soil.Low concentration tannic acid can improve the soil content of various forms of aluminum,but with the increase in the amount of tannic acid,the various forms of aluminum content are inhibited.With the soil pH increased,high concentrations tannic acid on the inhibition of the release of active aluminum increased.pH and tannic acid on the total amount of active aluminum garden soil was mutual weakening effect relationship.

Distribution of Forms of Sulphur and Their Relationships with Soil Attributes in Tea Growing Soils under Different Agro-climatic Zones of Northeastern India

Distribution of different forms of sulphur(S)and carbon-nitrogen-sulphur relationships were studied in surface and subsurface soils of some tea growing areas of Northeastern India.The soils were strongly acidic in reaction(pH-4.0 to 5.5),low to very high in organic carbon(4 to 54 g kg^-1),with cation exchange capacity(8.8 to 19.2 cmol(p+)kg^-1)and base saturation(50 to 77%).Organic S mostly contributed to the total-sulphur(62 to 77%)followed by Non sulphate S(28.8 to 37.2%)and sulphate S(0.7 to 1.4%).Except sulphate S,other forms of sulphur showed significant positive correlation among themselves as well as with organic carbon and total nitrogen.The C:N,C:S,N:S and C:N:S ratio varied from 8.2 to 10.0,6.18 to 71.57,0.62 to 7.26 and 100:10.1:1.4 to 100:12.2:16.2 respectively.Wider C:N:S ratios in all the surface and sub-surface soils indicated that the major portion of nitrogen and sulphur in tea growing soils of Northeastern India is locked up in organic combination which might pose as a potential threat towards tea plantation if application of sulphur is continuously ignored.

Distribution of Aluminum and Fluoride in Tea Plant and Soil of Tea Garden in Central and Southwest China

The distribution of Al and F contents and the relationship between Al and F in tea plants and soils of 12 tea gardens in Central and Southwest China were investigated from October 31 to November 14, 2006. The results show that there were differences in pH, CEC, the contents of organic matter (OM), Al and F in the different soils of the tea gardens. The Al content ranged from 1196 to 7976mg/kg for old leaf, 370 to 2681mg/kg for young leaf and 285 to 525mg/kg for stem, whereas the content of F ranged from 221 to 1504mg/kg for old leaf, 49 to 602mg/kg for young leaf and 13.5 to 77.5mg/kg for stem. The concentrations of labile Al varied obviously in the different soils, but the distribution law of labile Al content for the same garden was Alexchangeable≈AlFe-Mn oxide>Alorganic>Alwater-soluble. The contents of different labile F fractions varied slightly in the different soils and the different soil layers, though the exchangeable F content was lowest among the labile F in the soils. The concentrations of Al and F in tea plants increased with increasing amount of water-soluble Al or F, especially the amount of water-soluble fractions in the soil layer of 0-20cm.The correlation between Al content and F content in the tea leaf was more significant than that in the tea stem. Furthermore, the correlation between Al content and F content in whole tea plant was strongly significant (r=0.8763, p<0.01, n=36). There were evident tendency that Al concentration increased with the increase of F concentration in different soil layers. The correlation of water-soluble Al with water-soluble F in all soils was also strongly significant (r=0.7029, p<0.01, n=34). The results may provide a proof that Al and F are jointly taken up by tea plants to some extent in natural tea gardens.

Tea-Grown Soils and Tea Quality in Sichuan and Chongqing, China

Made teas and typical tea-grown soils in Sichuan and Chongqing were collected to investigate soil nutrients, related soil properties and tea quality. The tea-grown soils in Sichuan and Chongqing are distributed mainly in mountainous areas. The high annual precipitation (over 1100 mm), precipitous soil slopes, low cohesion among soil particles and high soil porosity suggested that intensive erosion and leaching might occur in these soils. Moreover, they were very acidic and poor in mineral nutrients such as N, P, K, Ca and Mg except S. The average content of total S was 20.40 g kg-1, much higher than that of organic matter in these soils, revealing that S in the tea-grown soils existed mainly in inorganic forms and very little in organic forms. Water-extractable S accounted for only a small amount of total S, which showed that most parts of sulfur in these soils were insoluble in W8ter. K and S varied greatly in made teas. The concentrations of N and P, however, varied little in these teas even though they differentiated significantly in the tea-grown soils. The high concentration of nitrogen in made teas could result in the high free amino acids and low polyphenol of teas. Significantly positive correlation was established between potassium and polyphenol in made teas. Teas with high ratio of phenol to free amino acids were usually good in taste and appearance.

Preliminary Study on Relationship Between Soil Parent Materials and Tea Quality

Six tea plantations with different soil-forming parent materials, the same tea variety and tea age and similar landforms and management were selected to conduct a systematic study on the relationship between soil properties and tea quality. The results showed that the quality of tea grown on the soils derived from granites, arenaceous shales, argillaceous sandstones, was superior; those on the soils derived from limestones,dolomites, Quaternary red clays, were inferior. Further study showed that sandy soils were beneficial to improving amino acid content of tea, and clayey soils made it decrease; high content of bases might decrease the contents of tea polypenols, caffeine, water extracts, but promote the content of amino acids; available phosphorous was significantly positively correlated with water extracts, but significantly negatively correlated with carbine; slowly available potassium was positively correlated with amino acid content. Soil parent materials should be regarded as an important factor in evaluating the adaptability of tea to soils.

ALUMINUM CONTENT OF TEA LEAVES AND FACTORSAFFECTING THE UPTAKE OF ALUMINUM FROMSOIL INTO TEA LEAVES

Numerous studies indicated that aluminum, the most abundant metallic element within the lithosphere, was consid- ered to be related to some human diseases especially the Alzheimer’s disease. Tea, economically an important beverage in the world, has been found to contain higher concentration of aluminum than many other drinks and foods. Therefore, tea would be a potentially important source of dietary aluminum. In order to understand the sources of aluminum in tea leaves and factors related with aluminum content of tea leaves, an experiment was designed to investigate the relationships of aluminum in tea leaves with leaf age, soil properties and forms of aluminum in soils. The results showed that there were great distinctions in the concentration of aluminum in tea leaves with different leaf age (Alold leaf>Almature leaf> Alyoung leaf). Moreover, soil pH was the major factor control- ling the uptake of aluminum from soil into tea leaves. Fnrthermore, the content of aluminum in tea leaves was better predicated by the soluble aluminum extracted by 0. 02mol/L CaCl2.

Detection and Evaluation of Metals in Tea and Soils of Tea Gardens in Mingshan

[ Objective] The study aimed to detect and assess metal ions in tea and soil of tea gardens in Mingshan County. [ Method] Seven metals （ including Pb, Cr, Cu, Ca, Mn, Mg and Zn） in soil and tea samples of tea gardens in Mingshan were determined by atomic absorption spectrometry, and the pollution situation of the soil and tea by Pb, Cr and Cu were evaluated using the single factor pollution index and Nemerow pollu- tion index, finally the accumulation of heavy metals in different soils were analyzed by the enrichment factor. I-Result] There was little difference for the same heavy metals in various soils, but different heavy metals had very significant difference. The enrichment factors of metals needed by tea growing, such as Ca, Mn, Mg and Zn, were higher than heavy metals like Pb and Cr. In addition, soils and tea of the six tea gardens were not polluted by Pb, Cr and Cu, and both single factor pollution index and Nemerow pollution index were below 0.7. [ Condusion] The research could provide scientific references for preventing tea from being polluted by heavy metals.

Effects of Soil Characters on the Tea Quality in the Tea Region of Qin-Ba Mountain,Hubei Province,PR China

Based on the analysis of physical and chemical properties of soil,nutrient elements and biochemical compositions of tea leaves,the relationship between soil characters and tea quality was studied.The results showed that soil total phosphorus,potassium,available potassium,copper and zinc were the main factors which affected and limited the content of nutrient elements of tea leaves.Furthermore,soil pH,organic matter,total nitrogen,potassium,exchangeable calcium,magnesium,available copper and zinc were the main factors affecting and limiting the content of biochemical compositions in tea leaves.Tea quality could be improved by controlling these soil factors.

Assessment of soil degradation and chemical compositions in Rwandan tea-growing areas

This study has focused on the processes of soil degradation and chemical element concentration in tea-growing regions of Rwanda,Africa.Soil degradation accelerated by erosion is caused not only by topography but also by human activities.This soil degradation involves both the physical loss and reduction in the amount of topsoil associated with nutrient decline.Soil samples were collected from eleven tropical zones in Rwanda and from variable depth within each collecting site.Of these,Samples from three locations in each zone were analyzed in the laboratory,with the result that the pH of all soil samples is shown to be less than 5（pH〈5） with a general average of 4.4.The elements such as iron（Fe）, copper（Cu）,manganese（Mn）,and zinc（Zn） are present in high concentration levels.In contrast calcium （Ca） and sodium（Na） are present at low-level concentrations and carbon（C） was found in minimal concentrations.In addition,elements derived from fertilizers,such as nitrogen（N）,phosphorous（P）,and potassium（K） which is also from minerals such as feldspar,are also present in low-level concentrations. The results indicate that the soil in certain Rwandan tea plantations is acidic and that this level of pH may help explain,in addition to natural factors,the deficiency of some elements such as Ca,Mg,P and N.The use of chemical fertilizers,land use system and the location of fields relative to household plots are also considered to help explain why tea plantation soils are typically degraded.

Determination of Essential &Toxic Metal and Its Transversal Pattern from Soil to Tea Brew

The soil pH of tea garden was acidic (4.6 - 5.3) with organic contents ranging between 0.91% - 1.09%. Available po- tassium concentration was predominantly found in the range of 167 - 208 mg/kg followed by the nitrogen (150 - 189 mg/ha) and phosphorus (42 - 169 mg/kg). The Fe content was the one of the predominant heavy metal in soil (5494 - 5627 mg/kg) followed by Mn (245 - 435 mg/kg), Zn (30 - 111 mg/kg) and Cu (22 - 51 mg/kg). The concentration of toxic heavy metals Pb, Ni, and Cd in tea leaves, made tea and its infusions were too low. The level of most abundant essential heavy metals in tea infusion was Zn ranging from 0.09 - 0.52 mg/kg followed by Mn 0.10 - 0.42 mg/kg. The level of Fe and Cu was ranging from 0.068 - 0.073 mg/kg and 0.007 - 0.04 mg/kg in tea infusion respectively. The levels of all es- sential and the toxic metals were thus obtained were compared well with the other parts of the world.

Survey on Fertility of Tea Garden Soil in Meizhou Region

Survey on fertility of tea garden soil in Meizhou region indicates that tea garden soil has strong acidity; organic matter content remains at medium level; there is a severe lack of available content of nitrogen(N),phosphorus(P) and potassium(K); available calcium(Ca) and magnesium(Mg) content is also insufficient; available sulfur(S) is abundant. In the management of tea cultivation,it is recommended to supplement organic fertilizer,balance the application of NPK fertilizer,and adequately alleviate shortage of Ca and Mg element,to guarantee high and stable yield of tea.

Spatial Analysis of Pollution-free Land for Tea Plantation and Comprehensive Evaluation of Soil Suitability

To survey the tea resource advantages in Guizhou's mountainous areas and complete rational layout of pollution-free tea planting areas, we take the case of Guiding County in Guizhou Province to establish the comprehensive evaluation indicator system of pollution-free land for tea plantation and comprehensive evaluation model of soil suitability of land for tea plantation according to the Environmental Conditions Standard for Pollution-free Food-Tea Producing Areas by the Ministry of Agriculture (NY 5020-2001), using land use data, remote sensing data, soil data, temperature, precipitation, light, DEM and other critical basic data, combined with the spatial analysis statistical method. The results show that the area of land suitable for planting tea is 1265 km2, accounting for 77.57% of the entire study area; the area of land with soil quality, climatic conditions and terrain conditions to meet the planting standard of pollution-free tea is 1232.51 km2, accounting for 75.57% of the total land area; the area of land most suitable for the planting of pollution-free tea is 128.71 km2, accounting for 7.89% of the total land area. The above studies can provide scientific basis and decision support for the implementation of tea planting industry standards in the specific areas, and provide reference for the comprehensive assessment of tea planting in other areas.

施用不同调理剂对酸化茶园土壤化学性质及茶叶品质的影响

针对茶园土壤酸化问题,利用不同配方调理剂对其进行土壤改良。采用田间微区试验的方法,以种植英红九号年限分别为2年(幼龄)和8年(成龄)茶园为研究对象,设置4个不同处理:不施调理剂(CK)、调理剂Ⅰ(生物炭,T1)、调理剂Ⅱ(生物炭+石灰,T2)及调理剂Ⅲ(生物炭+碱渣钙镁肥,T3),施用量为20 t·hm^(-2)。通过2年定位试验,研究3种调理剂对茶园土壤的主要化学性质及茶叶品质的影响。土壤分析表明,与对照组相比,2年的试验周期中,2种茶园的土壤pH均呈现先升后降的趋势,试验4个月后施用调理剂Ⅰ、Ⅱ、Ⅲ的幼龄茶园土壤pH值分别升高了0.11、0.20和0.22个单位,成龄茶园pH值分别升高了0.15、0.16和0.38个单位,但经过16个月后pH值又会逐渐下降。幼龄茶园土壤有效磷和速效钾含量均有显著升高,而成龄茶园只有速效钾含量显著升高。茶叶品质分析表明,与CK相比,试验4个月后幼龄茶园调理剂处理组茶叶的水浸出物含量分别显著增加6.66%、6.30%和6.80%。施用调理剂可以改善茶园酸性土壤,增加土壤肥力和茶叶内含物质含量。

Effects of Different Farming Methods and Their Effects on Soil Physicochemical Properties of Guilin Maojian Tea Gardens

In order to further optimize the cultivation and agronomic technology of Guilin Maojian tea gardens, the effects, efficiencies and costs of different farming methods and their effects on the physiochemical properties of tea garden soil were studied through the modification of the test sites with the non-farming land as the control. The results showed that human farming, mini-tiller farming and crawler tractor farming could improve the physical and chemical properties of soil. After farming, the soil had good water retention but low moisture content compared to the control group, while the soil bulk density and hardness value were significantly lower than those of the control, and the porosity of soil was significantly higher than that of contrast. With the passage of time, soil bulk density and hardness value gradually increased after farming, while the porosity of soil decreased gradually. There were great differences in the effects, efficiencies and costs of different farming methods. Crawler tractor had the best and most stable farming effect, and the operation efficiency was 10 times that of human farming while the cost was only 0.39 times of human farming. Therefore, it was feasible to adopt mini-tiller or crawler tractor to carry out mechanical farming of Guilin Maojian tea garden, which provided theoretical references for the soil property improvement using mechanical farming and was favorable for promoting the popularization of farming machines and the acceleration of mechanization of tea gardens. But for tea plantations that are intended to be mechanized, apart from the mechanical and technical personnel to be configured in place, the site conditions, planting modes and mechanical way reservation of tea garden should be planned accordingly. It is recommended to select flat or gentle slope for reclamation, and preference is given to non-sexual tea tree varieties with big line spacing over 180 cm long. Moreover, the main road construction should be more than 3.0 m, and trunk road 2.0 m or above. And isolation ditch and drain should be set between the tea garden and the surrounding mountain forests and farmland.

The Function of Roots of Tea Plant (<i>Camellia sinensis</i>) Cultured by a Novel Form of Hydroponics and Soil Acidification

A novel form of hydroponic culture was employed to explore the physiological function of roots of a tea plant (Camellia sinensis). The pH of the nutrient solution with an actively growing tea plant decreased during cultivation. Furthermore, no oxalic acid, tartaric acid, malic acid or citric acid, all possible factors in acidification, was detected in the nutrient solution of a growing plant. A proton pump inhibitor suppressed the acidification of the solution. Soil acidification might have been accelerated with a proton released from ammoniacal nitrogen preferentially for the growth, suggesting the specific mechanism of tea plant as a functional food.

有机种植方式对茶园土壤酸碱度的影响

土壤过度酸化是影响茶产业持续健康发展的主要因素之一,为了解有机管理方式对茶园土壤酸碱度的长期影响,以常规茶园为对照,研究了我国18个省份84个产茶县有机管理1~21 a对茶园土壤pH的影响。结果表明,有机茶园比常规茶园土壤pH平均高0.36,达极显著水平;随着有机管理年份的增加,茶园土壤pH总体为先显著上升、后显著下降并维持相对平稳,有机管理1~5、6~10、11~15、16~21 a的pH比常规茶园分别高0.48、0.23、0.28和0.30,均达显著或极显著水平;有机管理方式还有利于土壤pH向最适合茶树生长发育的方向发展,pH 4.5~5.5的茶园占比,常规茶园为41.9%,有机管理1~5、6~10、11~15、16~21 a的茶园分别为53.1%、48.9%、58.7%和66.7%,总体随有机管理年限的增加而增加。可见,有机管理方式能防止茶园土壤严重酸化,使土壤pH向着最适合茶树生长发育的方向发展。

不同海拔大叶种有机茶土壤与叶片碳氮磷生态化学计量特征

本试验对普洱市祖祥高山有机茶园有限公司的不同海拔梯度有机茶根际土壤和叶片碳(C)、氮(N)、磷(P)含量及其化学计量比进行了研究,探究了海拔高度对大叶种有机茶树根际土和叶片碳(C)、氮(N)、磷(P)生态化学计量特征的影响,分析了该试验地大叶种茶树生长的限制性因素,为大叶种有机茶园的合理养分管理提供数据支持。结果表明,不同海拔大叶种有机茶叶片的全碳、全氮、全磷含量及叶片C:N、C:P、N:P随海拔的升高有着不同的变化趋势:海拔越高,叶片的C:N和C:P越低,根据对植物营养元素的限制因素叶片N:P的分析可知,有机茶叶片的N:P在四个海拔梯度上都低于14,叶片全碳、全氮、全磷含量及叶片C:N、N:P在不同海拔间无显著差异,但叶片C:P在海拔间差异显著。不同海拔大叶种有机茶根际土壤全碳、全氮、全磷含量及土壤C:N、C:P、N:P随着海拔的升高有不同的变化趋势,海拔越高,土壤的全碳、全氮、全磷含量越高,在不同海拔间差异显著,土壤C:N、C:P、N:P在不同海拔间差异不显著。叶片的C:N、C:P、N:P与土壤的C:N、C:P、N:P多呈显著正相关关系。总之,大叶种有机茶的生长受氮的限制,所以在有机茶园经营管理过程中,要注意养分投入的平衡。

Impact of Seasons on Soil Physico-Chemical Properties and Tea Yields Based on Enriched Sheep Manure Used in Kericho, Kenya

Tea (Camellia sinensis (L) O. Kuntze) is a perennial crop grown for beverage consumption. Its production is the leading foreign exchange earner in Kenya contributing about 26% foreign exchange and 4% GDP. Nitrogen (N), Phosphorus (P) and potassium (K) are the recommended nutrients for tea production. However, their continuous use has been reported to have negative effect on soil physico-chemical properties and health. Sheep manure has been recommended in tea production for its soil pH buffering effect and increased tea yields. However, effects of sheep manure enriched with inorganic fertilizer on tea soils and yield have not been conclusively investigated. This study was established at KALRO—Tea Research Institute Kericho in Timbilil estate to determine the impact of seasonal variation on soil pysico-chemical properties and yields on enriched sheep manure used. Randomized complete block design (RCBD) experimental design was adopted with 14 treatments replicated thrice. The treatments were: control with no fertilizer use, NPK at 180 Kgs N/ha/yr, sheep manure only and two enriched sheep manure ratios of 4:1 and 8:1 which translates to a mixture of (4:1) 4 parts of sheep manure and 1 part of D.A.P inorganic fertilizer. Same applied to 8:1 ratio, respectively at rates of 60, 120, 180 and 240 Kgs N/ha once per year. Data were subjected to ANOVA in GenStat statistical package and means separated by Duncan’s multiple range tests at P ≤ 0.05 significance level. Results obtained showed that soil physico-chemical properties and tea yields were influenced significantly (P ≤ 0.05) by treatments applied. Soil available water and organic matter were higher on enriched sheep manure 4:1 at 240 rate used while soil porosity and bulk density were not significantly affected. Total N and P, increased with enriched sheep manure ratio of 4:1 at 240 rate while K+, Ca2+, Mg2+, Mn and pH increased with sole application of sheep manure at rate 180 and enriched sheep manure 8:1 at 180 rate. Tea yields increased with enriched sheep manure 4:1 ratios at the rates of 240. The findings recommend enriched sheep manure 4:1 ratio at 240 rates to be adopted by tea farmers within Kericho region. Further studies involving different geographical locations are proposed.

间作药用植物下陕南幼龄茶园小气候和土壤环境分析

为研究幼龄茶园中间作药用植物对茶园小气候和土壤状况的影响,以单作茶园为对照,间作元胡、紫云英和菘蓝3种药用植物为处理,测定茶园温湿度、土壤养分含量和土壤酶活性,并进行相关性分析。结果表明:间作药用植物可有效降低茶园空气温度,增加茶园空气湿度,其中菘蓝间作效果最佳;间作茶园土壤元素含量在不同发育时期均有所提高,土壤有效氮在成熟采收期含量高,有效磷在不同发育期含量都高,有效钾在发育中期含量高,且均高于单作茶园,其中菘蓝间作茶园土壤元素含量最高;在发育中期,菘蓝间作茶园的土壤脲酶和蔗糖酶活性显著高于其他间作类型。相关性分析表明土壤脲酶、土壤养分含量呈正相关,土壤蔗糖酶、土壤有效钾呈正相关,过氧化氢酶与土壤pH和有效钾呈正相关。茶园间作药用植物能明显改善幼龄茶园土壤和小气候环境,保障幼龄茶树生长,尽快达到采摘标准,使茶农及时获得收益。

接种蚯蚓对有机茶园土壤结构及有机碳库的影响

为探讨接种蚯蚓对有机茶园土壤团聚体、孔隙分布及有机碳库的影响,设置375条/m^(2),250条/m^(2),125条/m^(2)三种蚯蚓投放密度,将威廉腔环蚓接种于信阳有机茶园土壤中。结果表明:接种蚯蚓能显著提升>2mm大团聚体的比例而降低<0.25mm粒径的微团聚体的比例;提高水稳性小团聚体(0.25mm≤d≤2mm)比例,但对水稳性大团聚体d>2mm的影响不显著;高密度蚯蚓处理平均重量直径(MWD),平均几何直径(NWD)以及分型维数(D),团聚体破坏率(PAD)显著高于其他密度处理及对照(CK)。接种蚯蚓使得结构性孔隙(土壤颗粒间和团聚体间孔隙)所占比重增加,其中以团聚体间孔隙增加最为显著,其次为颗粒间孔隙,对由粘粒及矿物层间形成的毛管孔隙分布无显著影响;随着接种密度的增加,颗粒间孔隙度变化不显著而团聚体间孔隙度的相对值和绝对值均显著增加。添加蚯蚓能显著增加土壤活性有机库总量。接种密度的增加对活性有机碳库总量影响不大,但对土壤呼吸速率提升较多,故从温室气体排放角度考虑低接种密度更合适。可见,有机茶园中少量接种蚯蚓即能达到改良土壤结构的效果,并能缓解由于采茶造成的土壤压实问题。