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Research on the Construction of Intellectual Property System and Optimization of Business Environment in China
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作者 Zhang Xue Lyu Guofu 《科技与法律(中英文)》 2025年第1期125-137,共13页
With the rapid development of globalization and information technology,intellectual property has been one of the key drivers of economic growth,and the construction of intellectual property system has become an import... With the rapid development of globalization and information technology,intellectual property has been one of the key drivers of economic growth,and the construction of intellectual property system has become an important criterion for measuring the quality of business environment.This article is intended to explore the current status of intellectual property system construction in China,the challenges,and its relationship with the business environment,to propose the corresponding countermeasures and suggestions.The study finds that the legal system of intellectual property in China is gradually improving,and judicial and administrative protection are continuously strengthened.However,the challenges still remain such as frequent infringements,rights hard to protect and insufficient international cooperation.These issues not only affect the legitimate rights and interests of innovation entities,but also for the market fairness and the level of the business environment.Therefore,this article proposes that strengthening the perfection of the intellectual property legal system,enhancing intellectual property services and support capabilities,strengthening international cooperation and exchanges,and accelerating the cultivation of composite talents.It aims to provide theoretical references for the construction of intellectual property system and the optimization of the business environment,promote the high-quality development of economy and enhance the global competitiveness of the country. 展开更多
关键词 intellectual property business environment system construction economic development
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A Lonely Journey:An Autoethnography of the Fifteen-Year Identity Construction of a Female ESP Teacher in China
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作者 HAN Song 《Sino-US English Teaching》 2024年第8期343-353,共11页
This autoethnographic study explores from a sociocultural perspective the author’s identity construction journey as a female English-for-specific-purposes(ESP)teacher at a university in China for more than 15 years.I... This autoethnographic study explores from a sociocultural perspective the author’s identity construction journey as a female English-for-specific-purposes(ESP)teacher at a university in China for more than 15 years.It delves into the author’s evolving exercise between teacher agency and language policy within both institutional and national contexts.The author aims to voice for marginalized ESP teachers and create a space for them to better negotiate with English-for-general-purposes(EGP)colleagues,academic staff,and policy makers.This study highlights the significance of autoethnography in teacher identity research,particularly for niche ESP teachers who direct novel enterprises through bottom-up implementation.Furthermore,the author emphasizes humanism behind ESP instruction,which significantly expands the view that the ESP features instrumental value.She also advocates for comprehensive support to sustain ESP teaching innovation,ESP teachers’identity construction,and their professional development.These insights have implications for all accountable parties in the context of educational reform to enable constructive negotiation,for ESP teachers to develop their sense of empowerment,and for inclusive education to become more accessible. 展开更多
关键词 autoethnography teacher agency language policy ESP teacher identity
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Discussions on the Construction of“Double-Qualified”Teachers in Higher Vocational Colleges
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作者 Hong Ye Yuan Zhong Yanping Chen 《Journal of Contemporary Educational Research》 2024年第7期234-239,共6页
Creating a“double-qualified”teacher team is an important content in the construction of a higher vocational college teacher team.This paper probes into the significance of the construction of“double-qualified”teac... Creating a“double-qualified”teacher team is an important content in the construction of a higher vocational college teacher team.This paper probes into the significance of the construction of“double-qualified”teachers in higher vocational colleges,and deeply analyzes the problems existing in the construction of“double-qualified”teachers’training base,the institutionalization of“double-qualified”teacher rotation training,the regularization of cooperation with enterprises,and the rationalization of incentive mechanism.Therefore,it is of great practical significance to strengthen the theoretical research and practical exploration of the construction of“double-qualified”teachers. 展开更多
关键词 “Double-qualified”teachers Team construction Higher vocational colleges
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The Value and Realization Strategy of Teacher Ethics Construction in Colleges and Universities under the Perspective of“Cultivating People by Virtue”
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作者 LI Dan 《Journal of Literature and Art Studies》 2024年第8期741-747,共7页
“Cultivating people by virtue”has become the foundation of China’s education,and is the top priority of college education.“Only the learned can become teachers,and only those who behave properly can become role mo... “Cultivating people by virtue”has become the foundation of China’s education,and is the top priority of college education.“Only the learned can become teachers,and only those who behave properly can become role models”.The construction of teachers’ethics in colleges and universities is the premise of realizing the fundamental task of moral education,the need of teachers,the key to realizing the great rejuvenation of the Chinese nation,and it is also the way to correct the bad atmosphere in colleges and universities.In the construction of teachers’ethics,teachers should understand their roles and improve their professional ethics,schools should improve the guarantee system of teachers’ethics construction,meanwhile,improve the status of teachers and create a social environment of respecting teachers and valuing teachers. 展开更多
关键词 cultivating people by virtue teacher ethics colleges and universities
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Exploring the Long-Term Mechanism of the Construction of College Teachers’Ethics in the New Era Under the Concept of“Sanquan Education”
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作者 Jing Zuo Xiaoyue Liu 《Journal of Contemporary Educational Research》 2024年第1期140-146,共7页
This paper takes the long-term mechanism exploration of the construction of teachers’ethics in the new era under the concept of“Sanquan education,”aiming to explore how to establish a long-term mechanism of the con... This paper takes the long-term mechanism exploration of the construction of teachers’ethics in the new era under the concept of“Sanquan education,”aiming to explore how to establish a long-term mechanism of the construction to meet the needs of college education development under the background of the new era.A series of targeted measures and suggestions are put forward,including improving the system and mechanism,strengthening the education and training of teachers’ethics,and establishing the evaluation and assessment mechanism.The implementation of these measures can promote the formation of the long-term mechanism of the construction of teachers’ethics in colleges and universities,improve teachers’ethics and behavior quality,and provide strong support for the development of education in colleges and universities. 展开更多
关键词 Sanquan education ETHICS College education Long-term mechanism teacher quality
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Current Situation and Countermeasures of the Construction of “Double-Qualified” Teachers in Higher Vocational Colleges
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作者 Hong Ye Yuan Zhong Yanping Chen 《Journal of Contemporary Educational Research》 2024年第8期215-219,共5页
The construction of“double-qualified”teachers is the basic premise for the high-quality development of higher vocational education,and it is also the key force for higher vocational colleges to cultivate high-qualit... The construction of“double-qualified”teachers is the basic premise for the high-quality development of higher vocational education,and it is also the key force for higher vocational colleges to cultivate high-quality skilled talents.This paper probes into the positioning of the construction of“double-qualified”teachers in higher vocational colleges,deeply analyzes the current situation of the construction of“double-qualified”teachers in higher vocational colleges,and provides the countermeasures of the construction of“double-qualified”teachers. 展开更多
关键词 Higher vocational colleges “Double-qualified”teachers Current situation COUNTERMEASURE
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Construction of Ideological and Political Education in English Teacher Education Courses in Local Normal Universities:Objectives,Connotations,and Implementation Paths
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作者 Juanjuan Hong Jing Jiang 《Journal of Contemporary Educational Research》 2024年第9期47-52,共6页
The integration of ideological and political education into university curricula represents a new trend in holistic,comprehensive,and all-around education.The concept of“ideological and political education in English... The integration of ideological and political education into university curricula represents a new trend in holistic,comprehensive,and all-around education.The concept of“ideological and political education in English teacher education courses”embodies an interdisciplinary approach to curriculum reform.Grounded in the principle of fostering moral integrity,it aims to create high-quality courses by seamlessly integrating the transmission of knowledge and skills with the guidance of ideological values.This study explores the status quo,significance,and implementation pathways for integrating ideological and political education into English teacher education courses,providing valuable insights for future curricular development. 展开更多
关键词 Ideological and political education English teacher education courses Implementation paths
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Properties and Characteristics of Regolith-Based Materials for Extraterrestrial Construction 被引量:2
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作者 Cheng Zhou Yuyue Gao +4 位作者 Yan Zhou Wei She Yusheng Shi Lieyun Ding Changwen Miao 《Engineering》 SCIE EI CAS CSCD 2024年第6期159-181,共23页
The construction of extraterrestrial bases has become a new goal in the active exploration of deep space.Among the construction techniques,in situ resource-based construction is one of the most promising because of it... The construction of extraterrestrial bases has become a new goal in the active exploration of deep space.Among the construction techniques,in situ resource-based construction is one of the most promising because of its good sustainability and acceptable economic cost,triggering the development of various types of extraterrestrial construction materials.A comprehensive survey and comparison of materials from the perspective of performance was conducted to provide suggestions for material selection and optimization.Thirteen types of typical construction materials are discussed in terms of their reliability and applicability in extreme extraterrestrial environment.Mechanical,thermal and optical,and radiation-shielding properties are considered.The influencing factors and optimization methods for these properties are analyzed.From the perspective of material properties,the existing challenges lie in the comprehensive,long-term,and real characterization of regolith-based construction materials.Correspondingly,the suggested future directions include the application of high-throughput characterization methods,accelerated durability tests,and conducting extraterrestrial experiments. 展开更多
关键词 Extraterrestrial construction Characterization Mechanical property Thermal property Optical property Radiation-shielding
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A comprehensive review of lunar lava tube base construction and field research on a potential Earth test site 被引量:1
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作者 Yujie Feng Peng-Zhi Pan +3 位作者 Xuhai Tang Zhaofeng Wang Yuxin Li Altaf Hussain 《International Journal of Mining Science and Technology》 SCIE EI CAS CSCD 2024年第9期1201-1216,共16页
The Moon,as the closest celestial body to the Earth,plays a pivotal role in the progression of deep space exploration,and the establishment of research outposts on its surface represents a crucial step in this mission... The Moon,as the closest celestial body to the Earth,plays a pivotal role in the progression of deep space exploration,and the establishment of research outposts on its surface represents a crucial step in this mission.Lunar lava tubes are special underground caves formed by volcanic eruptions and are considered as ideal natural shelters and scientific laboratories for lunar base construction.This paper begins with an in-depth overview of the geological origins,exploration history,and distribution locations of lunar lava tubes.Subsequently,it delves into the presentation of four distinctive advantages and typical concepts for constructing bases within lava tubes,summarizing the ground-based attempts made thus far in lunar lava tube base construction.Field studies conducted on a lava tube in Hainan revealed rock compositions similar to those found during the Apollo missions and clear lava tube structures,making it a promising analog site.Lastly,the challenges and opportunities encountered in the field of geotechnical engineering regarding the establishment of lunar lava tube bases are discussed,encompassing cave exploration technologies,in-situ testing methods,geomechanical properties under lunar extreme environments,base design and structural stability assessment,excavation and reinforcement techniques,and simulated Earth-based lava tube base. 展开更多
关键词 Lunar lava tube Lunar base construction Extraterrestrial cave exploration Earth analog site
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Subsurface multi-physical characterization of mountain excavation and city construction in loess plateau with a fiber-optic sensing system 被引量:1
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作者 Jie Liu Bin Shi +3 位作者 Kai Gu Meng-Ya Sun Jun-Cheng Yao He-Ming Han 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2024年第8期2935-2946,共12页
Mountain excavation and city construction(MECC)projects being launched in the Loess Plateau in China involve the creation of large-scale artificial land.Understanding the subsurface evolution characteristics of the ar... Mountain excavation and city construction(MECC)projects being launched in the Loess Plateau in China involve the creation of large-scale artificial land.Understanding the subsurface evolution characteristics of the artificial land is essential,yet challenging.Here,we use an improved fiber-optic monitoring system for its subsurface multi-physical characterization.The system enables us to gather spatiotemporal distribution of various parameters,including strata deformation,temperature,and moisture.Yan’an New District was selected as a case study to conduct refined in-situ monitoring through a 77 m-deep borehole and a 30 m-long trench.Findings reveal that the ground settlement involves both the deformation of the filling loess and the underlying intact loess.Notably,the filling loess exhibits a stronger creep capability compared to underlying intact loess.The deformation along the profile is unevenly distributed,with a positive correlation with soil moisture.Water accumulation has been observed at the interface between the filling loess and the underlying intact loess,leading to a significant deformation.Moreover,the temperature and moisture in the filling loess have reached a new equilibrium state,with their depths influenced by atmospheric conditions measuring at 31 m and 26 m,respectively.The refined investigation allows us to identify critical layers that matter the sustainable development of newly created urban areas,and provide improved insights into the evolution mechanisms of land creation. 展开更多
关键词 Mountain excavation and city construction Fiber-optic monitoring Multi-physical characterization Compacted loess
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Research Progress and Prospects of Total Factor Productivity in the Construction Industry 被引量:1
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作者 Chen WANG Shijie YU +2 位作者 Chenhao LIANG Lidan CAI Xiaohong XIONG 《Asian Agricultural Research》 2024年第7期39-41,共3页
The high-quality development of the construction industry fundamentally stems from the significant improvement of total factor productivity.Therefore,it is of crucial significance for promoting the development of the ... The high-quality development of the construction industry fundamentally stems from the significant improvement of total factor productivity.Therefore,it is of crucial significance for promoting the development of the construction industry to a higher level by scientifically and accurately measuring the total factor productivity of the construction industry and deeply analyzing the influencing factors behind it.Based on a comprehensive consideration of research methods and influencing factors,this paper systematically reviews the existing relevant literature on total factor productivity in the construction industry,aiming to reveal the current research development trend in this field and point out potential problems.This effort aims to provide a solid theoretical foundation and valuable reference for further in-depth research,and jointly promote the continuous progress and development of total factor productivity research in the construction industry. 展开更多
关键词 construction industry Total factor productivity Research progress Influence factor Theoretical basis
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Intelligent Sensing and Control of Road Construction Robot Scenes Based on Road Construction
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作者 Zhongping Chen Weigong Zhang 《Structural Durability & Health Monitoring》 EI 2024年第2期111-124,共14页
Automatic control technology is the basis of road robot improvement,according to the characteristics of construction equipment and functions,the research will be input type perception from positioning acquisition,real... Automatic control technology is the basis of road robot improvement,according to the characteristics of construction equipment and functions,the research will be input type perception from positioning acquisition,real-world monitoring,the process will use RTK-GNSS positional perception technology,by projecting the left side of the earth from Gauss-Krueger projection method,and then carry out the Cartesian conversion based on the characteristics of drawing;steering control system is the core of the electric drive unmanned module,on the basis of the analysis of the composition of the steering system of unmanned engineering vehicles,the steering system key components such as direction,torque sensor,drive motor and other models are established,the joint simulation model of unmanned engineering vehicles is established,the steering controller is designed using the PID method,the simulation results show that the control method can meet the construction path demand for automatic steering.The path planning will first formulate the construction area with preset values and realize the steering angle correction during driving by PID algorithm,and never realize the construction-based path planning,and the results show that the method can control the straight path within the error of 10 cm and the curve error within 20 cm.With the collaboration of various modules,the automatic construction simulation results of this robot show that the design path and control method is effective. 展开更多
关键词 Scene perception remote control technology cartesian coordinate system construction robot highway construction
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Construction Activity Analysis of Workers Based on Human Posture Estimation Information
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作者 Xuhong Zhou Shuai Li +2 位作者 Jiepeng Liu Zhou Wu Yohchia Frank Chen 《Engineering》 SCIE EI CAS CSCD 2024年第2期225-236,共12页
Identifying workers’construction activities or behaviors can enable managers to better monitor labor efficiency and construction progress.However,current activity analysis methods for construction workers rely solely... Identifying workers’construction activities or behaviors can enable managers to better monitor labor efficiency and construction progress.However,current activity analysis methods for construction workers rely solely on manual observations and recordings,which consumes considerable time and has high labor costs.Researchers have focused on monitoring on-site construction activities of workers.However,when multiple workers are working together,current research cannot accu rately and automatically identify the construction activity.This research proposes a deep learning framework for the automated analysis of the construction activities of multiple workers.In this framework,multiple deep neural network models are designed and used to complete worker key point extraction,worker tracking,and worker construction activity analysis.The designed framework was tested at an actual construction site,and activity recognition for multiple workers was performed,indicating the feasibility of the framework for the automated monitoring of work efficiency. 展开更多
关键词 Pose estimation Activity analysis Object tracking construction workers Automatic systems
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Promoting grain production through high-standard farmland construction:Evidence in China
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作者 Shuai Hao Guogang Wang +4 位作者 Yantao Yang Sicheng Zhao Shengnan Huang Liping Liu Huanhuan Zhang 《Journal of Integrative Agriculture》 SCIE CSCD 2024年第1期324-335,共12页
Food security is a strategic priority for a country’s economic development.In China,high-standard farmland construction(HSFC)is an important initiative to stabilize grain production and increase grain production capa... Food security is a strategic priority for a country’s economic development.In China,high-standard farmland construction(HSFC)is an important initiative to stabilize grain production and increase grain production capacity.Based on panel data from 31 sample provinces,autonomous regions,and municipalities in China from 2005–2017,this study explored the impact of HSFC on grain yield using the difference-in-differences(DID)method.The results showed that HSFC significantly increased total grain production,which is robust to various checks.HSFC increased grain yield through three potential mechanisms.First,it could increase the grain replanting index.Second,it could effectively reduce yield loss due to droughts and floods.Last,HSFC could strengthen the cultivated land by renovating the low-and medium-yielding fields.Heterogeneity analysis found that the HSFC farmland showed a significant increase in grain yield only in the main grain-producing areas and balanced areas.In addition,HSFC significantly increased the yields of rice,wheat,and maize while leading to a reduction in soybean yields.The findings suggest the government should continue to promote HSFC,improve construction standards,and strictly control the“non-agriculturalization”and“non-coordination”of farmland to increase grain production further.At the same time,market mechanisms should be used to incentivize soybean farming,improve returns and stabilize soybean yields. 展开更多
关键词 high-standard farmland farmland construction food security food production area DIFFERENCE-IN-DIFFERENCES
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Application of Modern Construction Art in Landscape Architecture Planning and Design
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作者 JIANG Yuanyuan 《Journal of Landscape Research》 2024年第3期43-47,共5页
The continuous progress of urbanization has driven the continuous development and innovation of landscape planning and design.Focused on the important design method of modern construction art,this study analyzed its c... The continuous progress of urbanization has driven the continuous development and innovation of landscape planning and design.Focused on the important design method of modern construction art,this study analyzed its concepts and characteristics,and made deep exploration to its application in landscape planning and design.The results indicated that modern construction art had a significant impact on landscape spatial planning and layout,spatial design forms,and spatial ornaments.The use of modern construction art concepts could make landscape design more scientific,artistic,and humane,creating higher quality leisure and entertainment venues for audiences. 展开更多
关键词 Landscape architecture design Modern construction art construction Art
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Friction Characteristics Between Marine Clay and Construction Materials
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作者 KOU Hailei HUANG Jiaming CHENG Yang 《Journal of Ocean University of China》 CAS CSCD 2024年第2期427-437,共11页
Structure-soil interface friction characteristics is of importance to investigate the interaction between engineering structures and soils,especially for offshore structures.The interface friction behavior between mar... Structure-soil interface friction characteristics is of importance to investigate the interaction between engineering structures and soils,especially for offshore structures.The interface friction behavior between marine clay and structural materials with different roughness was studied in this paper by using 3D optical scanning tests,a modified direct shear device and numerical simulation.Relationships between the surface roughness of structures,water content and interface friction angle were presented by model tests.The increase of water contents decreased the interface friction angles.For interfaces with different roughness,the interface friction angles will be smaller than that of the soil when the water content exceeds a certain value.The roughness of the interface and the water content of the soil are mutually coupled to influence the coefficient of friction(COF).This paper proposed a Finite Element Method(FEM)to simulate the interface direct shear tests of structures with different roughness.The surface models with different roughness are established based on the structure data obtained by 3D scanning.The Coupled Eulerian-Lagrangian(CEL)approach was employed to analyse soils sheared by irregular surfaces.The interface behavior for interfaces with different roughness under cyclic shear stresses was analyzed by FEM. 展开更多
关键词 marine clay construction material interface friction behavior surface roughness Finite Element Method
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Research progress on the adaptability of lunar regolith simulant-based composites and lunar base construction methods
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作者 Bo Liu Peng Sun +2 位作者 Wei Yao Tao Li Wei Xu 《International Journal of Mining Science and Technology》 SCIE EI CAS CSCD 2024年第10期1341-1363,共23页
The development and utilization of lunar resources are entering a critical stage.Immediate focus is needed on key technologies for in-situ resource utilization(ISRU)and lunar base construction.This paper comparatively... The development and utilization of lunar resources are entering a critical stage.Immediate focus is needed on key technologies for in-situ resource utilization(ISRU)and lunar base construction.This paper comparatively analyzes the basic characteristics of lunar regolith samples returned from Chang'e-5(CE-5),Apollo,and Luna missions,focusing on their physical,mechanical,mineral,chemical,and morphological parameters.Given the limited availability of lunar regolith,more than 50 lunar regolith simulants are summarized.The differences between lunar regolith and simulants concerning these parameters are discussed.To facilitate the construction of lunar bases,this article summarizes the advancements in research on construction materials derived from lunar regolith simulants.Based on statistical results,lunar regolith simulant-based composites are classified into 5 types by their strengthening and toughening mechanisms,and a comprehensive analysis of molding methods,preparation conditions,and mechanical properties is conducted.Furthermore,the potential lunar base construction forms are reviewed,and the adaptability of lunar regolith simulant-based composites and lunar base construction methods are proposed.The key demands of lunar bases constructed with lunar regolith-based composites are discussed,including energy demand,in-situ buildability,service performance,and structural availability.This progress contributes to providing essential material and methodological support for future lunar construction. 展开更多
关键词 In-situ resource utilization Lunar regolith Lunar base construction Lunar regolith simulant-based composites
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Work-Related Accidents on a Hospital Construction Site in Benin
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作者 Adjobimey Mênonli Mikponhoué Rose +5 位作者 Dégbey Cyriaque Hountohotègbé Esdras Guédou Alexandre Lawani Affousath Ayélo Paul Hinson Antoine 《Occupational Diseases and Environmental Medicine》 2024年第2期142-159,共18页
Introduction: Work-related accidents are frequent and serious in the construction sector. The aim of the study was to determine the frequency and factors associated with occupational accidents on the construction site... Introduction: Work-related accidents are frequent and serious in the construction sector. The aim of the study was to determine the frequency and factors associated with occupational accidents on the construction site of a referral hospital in Benin. Methods: A cross-sectional study was carried out. The sample size was calculated using the Schwartz form adjusted for the number of workers on site and was 129 workers. Random sampling was used. The dependent variable was work-related accidents. The other variables were socio-demographic and occupational characteristics. Data were collected through a questionnaire survey. Medians and proportions were calculated. An association was sought using Chi-square and Fisher tests with a threshold of p Results: A total of 132 workers were included. Their median age was 30 years with an ITQ of [27 - 38];men were the most represented 126 (95.45%) with a level of education higher than or equal to high school in 101 (76.52%) and in the majority with a permanent status 85 (64.39%). Seniority of more than 5 years was observed in 92 (69.7%). Workers working more than 8 hours of overtime per week numbered 57 (43.18%). Exposure to vibrating objects was 49 (37.12%). In terms of psychosocial constraints, 82.58% had high psychological demands;79.53% low decision-making latitude;50.76% low social support. The frequency of work-related accidents was 6.82%, and the only associated factor was the type of worker (p = 0.016). On the other hand, there were 10.2% accidents among workers handling vibrating objects versus 4.98% among those not using them. With regard to psychosocial constraints, the following frequencies were recorded respectively: 6.42% among those with high psychological demand versus 8.7% among those with low psychological demand;7.62% among those with low decision-making latitude versus 3.7% among those with high decision-making latitude;8.96% among those with low social support versus 4.62% among those with high support. Conclusion: Work-related accidents on construction sites must be avoided by all possible means including the management of psychosocial constraints. 展开更多
关键词 Occupational Injury Building and Civil Engineering construction ENVIRONMENT
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“Instruction-Category” Approach of Test Suite Construction for Oven Embedded System
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作者 Mengqing Tanli Jiyi Xiao Ying Zhang 《Journal of Software Engineering and Applications》 2024年第9期713-730,共18页
Being different from testing for popular GUI software, the “instruction-category” approach is proposed for testing embedded system. This approach is constructed by three steps including refining items, drawing instr... Being different from testing for popular GUI software, the “instruction-category” approach is proposed for testing embedded system. This approach is constructed by three steps including refining items, drawing instruction-brief and instruction-category, and constructing test suite. Consequently, this approach is adopted to test oven embedded system, and detail process is deeply discussed. As a result, the factual result indicates that the “instruction-category” approach can be effectively applied in embedded system testing as a black-box method for conformity testing. 展开更多
关键词 “Instruction-Category” Approach Test Suite construction Embedded System
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Cloning of BjNAC102 Promoter and Construction of Expression Vector in Brassica juncea
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作者 XIAO Xin-bo YUAN Yu-hui +5 位作者 YOU Liang HUANG Xin CHEN Ming-zhe MA Yin-hua LIU Xian-jun CHEN Hu 《Agricultural Science & Technology》 CAS 2024年第3期14-20,共7页
Transcription factor NAC102 plays an important role in the abiotic stress responses of plants.In this study,the promoter sequence of 3000 bp located in the upstream of the BjNAC102 gene was cloned from Brassica juncea... Transcription factor NAC102 plays an important role in the abiotic stress responses of plants.In this study,the promoter sequence of 3000 bp located in the upstream of the BjNAC102 gene was cloned from Brassica juncea‘Sichuan Yellow Seed’by using the homologous cloning method.The expression vector of the GUS gene driven by the BjNAC102 promoter was constructed by seamless cloning technology.The results showed that the sequence of the promoter of the BjNAC102 gene contained many cis-acting elements involved in light responsiveness,gibberellinresponsive element,and auxin-responsive element.It was speculated that BjNAC102 played an important role in the abiotic stress response in Brassica juncea.The expression vector of the promoter of the BjNAC102 gene was constructed,which layed a foundation for further studies of the expression pattern of the BjNAC102 gene in Brassica juncea. 展开更多
关键词 Brassica juncea PROMOTER BjNAC102 Plant expression vector construction
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