Design and Application of“STEM+”6E Teaching Mode in Information Technology Teaching

The concept of“STEM+”integrates art,humanistic literacy,and social values in the traditional STEM education concept,advocates cross-disciplinary integration,and aims to cultivate compound talents equipped to tackle future challenges.In 2022,the Ministry of Education issued the“Compulsory Education Information Technology Curriculum(2022 Edition),”emphasizing the core literacy of information science and technology and the integration of interdisciplinary disciplines,and encouraging the teaching mode suitable for discipline characteristics.The 6E teaching mode is a student-centered teaching strategy characterized by active exploration and cross-disciplinary integration.This article innovatively designed the“STEM+”6E teaching mode,which is applied to junior high school information technology teaching,which can better achieve core literacy teaching goals.

Exploration on 5E Teaching Principle-based Education for the Object-oriented Software Construction Course

Most current object-oriented programming courses offered by domestic colleges and universities generally focus on the object-oriented programming language itself,i.e.,the programming grammar of the language,but ignore the design pattern.However,design patterns are essential to software engineering because they can solve common problems in software design and improve code reuse,readability,extensibility,and reliability.Our Object-oriented Software Construction Course is creative since it aims at cultivating students’object-oriented thinking as well as basic abilities required to construct high-quality,object-oriented software.Specifically,we exploit the 5E teaching principle during the education of this course,and present the whole pipeline in the paper.We also provide one case of the factory pattern to further demonstrate the implementation of the 5E teaching principle in the course.The effect of the 5E teaching principle has also been demonstrated.

Exercise-induced modulation of miR-149-5p and MMP9 in LPS-triggered diabetic myoblast ER stress: licorice glycoside E as a potential therapeutic target

Background:This study explores the relationship between endoplasmic reticulum(ER)stress and diabetes,particularly focusing on the impact of physical exercise on ER stress mechanisms and identifying potential therapeutic drugs and targets for diabetes-related sepsis.The research also incorporates traditional physical therapy perspectives,emphasizing the genomic insights gained from exercise therapy in disease management and prevention.Methods:Gene analysis was conducted on the GSE168796 and GSE94717 datasets to identify ER stress-related genes.Gene interactions and immune cell correlations were mapped using GeneCard and STRING databases.A screening of 2,456 compounds from the TCMSP database was performed to identify potential therapeutic agents,with a focus on their docking potential.Techniques such as luciferase reporter gene assay and RNA interference were used to examine the interactions between microRNA-149-5p and MMP9.Results:The study identified 2,006 differentially expressed genes and 616 miRNAs.Key genes like MMP9,TNF-α,and IL1B were linked to an immunosuppressive state.Licorice glycoside E demonstrated high affinity for MMP9,suggesting its potential effectiveness in treating diabetes.The constructed miRNA network highlighted the regulatory roles of MMP9,IL1B,IFNG,and TNF-α.Experimental evidence confirmed the binding of microRNA-149-5p to MMP9,impacting apoptosis in diabetic cells.Conclusion:The findings highlight the regulatory role of microRNA-149-5p in managing MMP9,a crucial gene in diabetes pathophysiology.Licorice glycoside E emerges as a promising treatment option for diabetes,especially targeting MMP9 affected by ER stress.The study also underscores the significance of physical exercise in modulating ER stress pathways in diabetes management,bridging traditional physical therapy and modern scientific understanding.Our study has limitations.It focuses on the microRNA-149-5p-MMP9 network in sepsis,using cell-based methods without animal or clinical trials.Despite strong in vitro findings,in vivo studies are needed to confirm licorice glycoside E’s therapeutic potential and understand the microRNA-149-5p-MMP9 dynamics in real conditions.

Research and Practice of the P.E. Information Teaching Design System

In this paper, the physical education information teaching design system is researched and developed based on VB6.0 optimization platform. According to the national physical education and health course standards and the fundamental theories of the constructivisrn, the author adopts the modular-design development method to design two functional areas which are the displaying of the teaching design contents and the online information database （nine modules in total） on the basis of the P.E. teaching design flow. Importantly, this system features the compatibility, scalability and practical applicability, and can effectively improve the ability of P.E. teachers to design their teaching, and hence provides a guarantee for the improvement of teaching quality and efficiency.

Analysis on the Optimization of the P.E. Course Teaching Plan in Colleges and Universities

In the new period, the physical education in colleges and universities is of greater and greater significance for the enhancement of students＇ learning efficiency. In this paper, the current situation of the current physical education is analyzed, and also related suggestions on the optimization of the P.E. course teaching plan are proposed.

Introduction of Internet P.E News Reporter

Consistent with previous research, results showed men get more paragraphs and photography coverage. Interestingly, women's factual information is always associated with relevant men report, including P.E relevant with personal information.

Development Characteristics of Private P.E Economy

The regional policy in the innovation development of private P.E economy plays better role in management according to law,guidance and escort;increase the macro quality of main body of private P.E entrepreneurs and the overall staff quality,develop P.E vocational P.E to provide P.E talents for private P.E economy.

Research and Development of Multimedia Teaching Management System Based on SIP ＆ P2P

Multimedia teaching managemem system based on SIP ＆ P2P is proposed on account of the existing deficiency on video teaching of distance education. The designed P2P-SIP system applies SIP protocol to realize the underlying DHT, which guarantees zero-configuration, robustness and adaptability in P2P system. And maximum improvement in performance of the networks for multimedia system can be implemented by rapid networking and information sharing technology of the underlying P2P networks as well as fast call and location technology of SIP system. Experiments indicates that multimedia teaching management system based on SIP ＆ P2P could meet the requirement of system design on data latency and calling location.

清肾颗粒对大鼠NRK-52E细胞转分化模型miR-23b和PINK1/Parkin通路的影响

目的 探讨TGF-β1诱导大鼠NRK-52E细胞转分化模型中miR-23b对PINK1/Parkin通路的靶向调节机制,并阐明清肾颗粒含药血清对NRK-52E细胞转分化的干预机理。方法 采用超高效液相色谱(UPLC)指纹图谱法对清肾颗粒进行全指纹图谱分析。构建TGF-β1诱导大鼠NRK-52E细胞转分化模型,转染siRNA后分为模拟物空载对照组、miR-23b-5p模拟物组、抑制剂空载对照组、miR-23b-5p抑制剂组,观察miR-23b-5p对PINK1表达量的影响。再将NRK-52E细胞分组为正常组、TGF-β1组、清肾颗粒组、miR-23b-mimic-NC组、miR-23b-mimic组、miR-23b-mimic+清肾颗粒组,Western blot法检测NRK-52E细胞中Pink1、Parkin、LC3Ⅱ、Beclin-1、P62、α-SMA蛋白表达,RT-qPCR法检测NRK-52E细胞中miR-23b-5p、Pink1、Parkin、Beclin-1、α-SMA mRNA的表达,双荧光素酶报告基因实验检测miR-23b-5p与PINK1的靶向关系。结果 UPLC指纹图谱法鉴定出清肾颗粒中11个活性成分。miR-23b-5p过表达后,PINK1 mRNA表达量也显著增加(P<0.05);而miR-23b-5p表达沉默后,PINK1 mRNA表达量也显著减少(P<0.05)。双荧光素酶报告显示,Rno-miR-23b-5p能显著下调Rno-PINK1-WT荧光素酶活性(P<0.05),但未能下调突变Rno-PINK1-mut荧光素酶活性(P>0.05)。清肾颗粒含药血清干预实验发现,TGF-β1组的miR-23b-5p、Pink1、Parkin、Beclin-1、LC3Ⅱ表达及LC3Ⅱ/Ⅰ比值均明显低于正常组,P62和α-SMA表达明显高于正常组(P<0.05)。清肾颗粒组和miR-23b-mimic组的miR-23b-5p、Pink1、Parkin、Beclin-1、LC3Ⅱ表达及LC3Ⅱ/Ⅰ比值均明显高于TGF-β1组,P62和α-SMA表达明显低于TGF-β1组(P<0.05)。miR-23b-mimic+清肾颗粒组的表现更优于miR-23b-mimic组(P<0.05)。结论 清肾颗粒能够上调NRK-52E细胞内miR-23b-5p表达,并通过增强PINK1/Parkin通路介导的线粒体自噬活性,抑制NRK-52E细胞转分化进程。

Study on the Anti-Inflammatory and Analgesic Mechanisms of Breast Lump Resolution Detergent in a Rat Model of Breast Hyperplasia

Objective: This study aimed to investigate the anti-inflammatory and analgesic mechanisms of a breast lump resolution detergent in a rat model of breast hyperplasia. Methods: A rat model of breast hyperplasia was established using injections of estradiol benzoate combined with progesterone. The effects of the breast lump resolution detergent on nipple height and diameter in the rat model were observed, along with its impact on serum levels of estradiol (E2), prolactin (PRL), and progesterone (P). Additionally, the study examined the morphological changes in breast tissue. The impact of the breast nodule detergent on blood rheology parameters was also observed. Furthermore, the anti-inflammatory effect of the breast nodule detergent was assessed using the cotton ball granuloma experiment, and the analgesic effect was observed using the writhing test. Results: The breast lump resolution detergent reduced nipple height and diameter in the rat model, decreased serum levels of E2, PRL, and P, and alleviated pathological changes in breast tissue. It also lowered hemorheological parameters including whole blood high, medium, and low shear viscosity, plasma viscosity, red blood cell hematocrit, red blood cell aggregation index, red blood cell deformability index, red blood cell electrophoresis time, and erythrocyte sedimentation rate in the acute “blood stasis” rat model. The detergent reduced the weight of cotton ball granulomas in mice and decreased the number of writhing episodes caused by acetic acid. Conclusion: The breast lump resolution detergent demonstrates favorable therapeutic effects in treating breast hyperplasia, promoting blood circulation and removing blood stasis, exerting anti-inflammatory properties, and providing analgesic effects. The downregulation of serum E2 and PRL levels and the upregulation of P levels may be critical mechanisms underlying its efficacy.

Linc01419调控miR-34a-5p/E2F3轴促进膀胱癌细胞增殖与侵袭

目的:探讨长链非编码RNA Linc01419对膀胱癌细胞增殖和侵袭的影响及作用机制。方法:通过UALCAN软件(https://ualcan.path.uab.edu/)分析TCGA数据库中Linc01419在膀胱癌组织与正常膀胱组织中的表达差异;采用实时荧光定量聚合酶链反应(real-time fluorescence quantitative polymerase chain reaction,RT-qPCR)检测Linc01419在不同膀胱癌细胞系、22例经病理证实为膀胱癌的手术患者的肿瘤组织及癌旁正常膀胱组织中的表达水平;应用细胞增殖/毒性检测(cell counting kit-8,CCK-8)实验和Transwell小室侵袭实验检测敲低Linc01419表达对膀胱癌细胞增殖与侵袭的影响;采用双荧光素酶报告基因检测法分析Linc01419与miR-34a-5p及miR-34a-5p与E2F3之间的靶向调控关系。结果:UALCAN数据库分析显示相较正常膀胱组织,Linc01419在膀胱癌组织中显著高表达(P<0.001);RT-qPCR分析结果显示相较癌旁正常膀胱组织,Linc01419在22例膀胱癌组织中表达明显上调(P<0.001),与UALCAN数据库分析结果一致;Linc01419在4株膀胱癌细胞中的表达明显高于正常膀胱上皮细胞(P<0.01);敲低Linc01419表达,可显著抑制膀胱癌细胞的增殖、侵袭及N-cadherin、PCNA蛋白的表达,而显著促进E-cadherin的蛋白表达(P<0.05);miR-34a-5p过表达对膀胱癌细胞具有类似的抑制作用;双荧光素酶报告基因实验、RIP及Pull-down实验证实Linc01419可靶向结合miR-34a-5p,而后者进一步介导了对E2F3的靶向调控;抑制miR-34a-5p表达,可显著削弱Linc01419沉默对膀胱癌细胞生物学行为及E-cadherin、N-cadherin、PCNA、E2F3表达的影响。结论:Linc01419在膀胱癌中异常高表达,其通过调控miR-34a-5p/E2F3轴促进膀胱癌细胞增殖和侵袭,很可能是膀胱癌发生、发展过程中的一个重要环节。

基于ADDIE模型的沉浸式电子商务教学实践探索

我国电子商务行业方兴未艾,其专业人才的需求旺盛,因而许多高职院校都开设有电子商务专业。但与此同时,高职电子商务专业的毕业生往往就业率偏低且专业不对口的问题一直存在。不少民营企业来校园招聘电子商务人才往往找不到匹配工作岗位的人才。本文分析了造成这样问题的原因,并提出了通过ADDIE模型进行沉浸式教学设计的应对措施。

线上线下双模式教育的应用——以机械电子工程专业必修课《热力学与传热学基础》为例

后疫情时代,为适应教学要求,逐渐衍生出线上线下双模式的教学方式。本文以机械电子工程专业的必修课《热力学与传热学基础》为例介绍了线上线下双模式教育在教学中的应用。首先介绍了《热力学与传热学基础》这门专业必修课的主要授课内容、学习目标和考核标准,其次以理想气体基本热力学过程的p-v图和T-s图为例,说明了双模式教育的应用为教学带来的好处。

基于PBL模式的电子商务专业教学改革探究——以移动电子商务课程为例

为适应新时代行业发展需求,促进电子商务专业学生的跨学科知识整合,提高实践和创新能力,在移动电子商务课程中运用PBL教学模式,通过项目任务为学生提供跨学科学习机会,确立学习目标,培养解决问题的意识。教学过程中运用灵活的教学策略,注重建立学生在学习中的主体地位,以跨学科问题研讨促进建立多学科视角,有效地组织课堂引导学生交流与反思。教学实践结果表明,PBL教学模式能够提高学生学习主动性,对多学科知识整合和互动交流方面具有良好的促进作用。提出在PBL教学中应注重提升学生自主发现问题的能力,引导学生参与科研活动,引入多主体评价机制等改进策略。

修正磁化模型的多组分铁磁性颗粒运动研究

铁磁性颗粒因具有铁磁性被广泛应用于化工环保、生化工程和能源等各个领域,磁场具有的穿透性质,对于采用铁磁性颗粒的系统,可通过改变磁场控制系统内颗粒的运动状态.文章基于传统的磁化模型,采用相对参考系转换方法,提出了适用范围更广的修正P-E磁化模型,可以计算铁磁性颗粒在任意方向磁场作用下所受磁化力.通过有限体积法(FVM)与离散单元法(DEM)耦合进行数值模拟,验证了修正P-E磁化模型的精确性,并模拟多组分颗粒在磁场中的运动,对比了铁磁性颗粒与惰性颗粒在不同配比及不同磁场条件下的运动特性,对颗粒分布、颗粒速度矢量和颗粒总能量变化3个方面进行分析.结果表明:在多组分颗粒系统中,铁磁性颗粒依旧保持成链特性,但成链速度与长度降低;随着铁磁性颗粒占比提高,铁磁性颗粒初始能量增大,聚链数量与成链长度将有所增加,约束惰性颗粒能力增强;此外,施加水平与竖直方向磁场时,多组分颗粒系统达到稳定速度最快,可以通过增大铁磁性颗粒占比有效提升稳定速度,使系统更快趋于稳定;而施加含有倾角的磁场时,随着铁磁性颗粒占比升高,铁磁性颗粒达到稳定状态需要的时间逐渐降低,较难通过改变铁磁性颗粒占比缩短稳定所需时间.

雨课堂融合OBE理念在高职电子商务类课程混合式教学的实践优化策略

文章以服务乡村振兴为背景,聚焦高职电子商务类课程混合式教学改革,结合OBE教育理念重构课程内容,提出基于雨课堂教学平台实施项目化教学、七步教学法,并构建多元评价体系。全过程融入思政元素实现“思创融合”,推进岗课赛证综合育人,并针对院校、教师、学生提出优化实践效果的具体策略。

p16/Ki-67双染检测、HPVE6/E7mRNA检测在宫颈病变诊断中的价值研究

目的:探讨p16/Ki-67双染检测、HPVE6/E7mRNA检测在宫颈病变诊断中的价值。方法:选择2021年4月至2023年2月我院收治的605例高危型HPV阳性患者为研究对象,所有患者入院后均进行阴道镜检查与病理活检,取患者细胞学样本分别进行p16/Ki-67双染检测、HPVE6/E7mRNA检测。比较两种方法单独检查与联合检测对宫颈病变阳性检出率的差异;比较三种检查方法对宫颈病变诊断效能的差异。结果:联合检测检出CIN1~3阳性率均高于p16/Ki-67双标记、HPVE6/E7mRNA检测(P<0.05);三种检测方式的浸润癌阳性检出率比较无明显差异(P>0.05)。Kappa检验分析结果显示,p16/Ki-67双标记与HPVE6/E7mRNA检查结果与病理诊断结果具有较好的一致性(Kappa值=0.719,P<0.05);联合检测结果与病理诊断结果具有高度的一致性(Kappa值=0.894,P<0.05)。联合检测对宫颈病变的诊断灵敏度、特异性与准确率高于p16/Ki-67双标记与HPVE6/E7mRNA检查(P<0.05)。结论:p16Ki-67双染与HPVE6/E7mRNA联合检测可准确检出宫颈病变程度,对宫颈病变疾病类型的鉴别可提供充足可靠的数据支持,联合检查的诊断效能较好,对患者后续相关治疗开展有积极指导意义。

基于学习通平台的线上线下教学模式探索与实践

针对当前传统教学忽略学生主体地位、教学方式单一、忽视个体差异等问题,文章梳理了混合式教学模式的研究进展,针对《电子商务数据分析》课程,提出从课程目标、教学模式、教学内容、实验设计和评价方式等方面开展线上线下结合的混合式教学模式,通过“数据分析模型”教学内容对混合式教学模式进行了深入分析。文章旨在为线上线下混合式教学课程提供参考和借鉴。

miR-150-5p靶向ZEB1调控EMT对子宫内膜癌细胞恶性生物学行为的影响

目的探讨微小RNA-150-5p(miR-150-5p)是否可靶向锌指E盒结合蛋白1(ZEB1)调控子宫内膜癌(EC)细胞上皮间质转化(EMT)进而影响癌细胞的恶性生物学行为。方法RT-qPCR技术检测正常子宫内膜和EC组织中、子宫内膜上皮细胞系hEEC及EC细胞系Ishikawa和HEC-1-A中miR-150-5p相对表达量。过表达miR-150-5p,MTT法、菌落形成实验、伤口愈合实验和Transwell实验分别评估Ishikawa细胞活力、克隆形成、迁移及侵袭能力;双荧光素酶报告基因实验验证miR-150-5p与ZEB1的靶向关系;RT-qPCR检测miR-150-5p及ZEB1 mRNA相对表达量;Western blot技术检测ZEB1、E-钙黏蛋白(E-cadherin)、N-钙黏蛋白(N-cadherin)、波形蛋白(Vimentin)及基质金属蛋白酶9(MMP-9)蛋白表达量。结果与正常子宫内膜组织比较,EC组织中miR-150-5p相对表达量降低(P<0.05);与hEEC细胞比较,HEC-1-A细胞和Ishikawa细胞中miR-150-5p相对表达量降低(P<0.05),Ishikawa细胞中最低(P<0.05)。与空白组比较,miR-150-5p mimics组细胞490 nm处吸光度值、细胞菌落数、迁移数和侵袭数、ZEB1 mRNA和蛋白相对表达量及N-cadherin、Vimentin和MMP-9蛋白相对表达量显著降低,miR-150-5p相对表达量、E-cadherin蛋白相对表达量显著升高(P<0.05)。经生物信息学分析,ZEB1被预测为miR-150-5p的潜在靶基因。结论miR-150-5p可靶向ZEB1抑制癌细胞的恶性生物学行为,其作用机制可能与调控EC细胞EMT进展有关。

S-FHMC710型煤泥重介质旋流器在吕家坨选煤厂的应用

吕家坨矿选煤厂为了解决粗煤泥分选效果差的问题,采用S-FHMC710型超级煤泥重介质旋流器分选粗煤泥,并通过工业性试验对超级煤泥重介质旋流器进行工艺性能进行检测。试验结果表明:大于0.5 mm粒级可能偏差0.050 kg/L,数量效率99.64%;0.50~0.25 mm粒级可能偏差0.060 kg/L,数量效率98.76%;0.25~0.10 mm粒级可能偏差0.087 kg/L,数量效率96.99%,有效分选下限达到0.10 mm。该超级煤泥重介质旋流器应用效果良好,对于提高精煤质量、产率和选煤厂经济效益具有显著的效果。