Exploring the mechanism of action of herb pair Pinellia Ternata-Magnolia Officinalis in the treatment of liver cancer based on network pharmacology and molecular docking

Background:Explore the anti-tumor mechanism of herb pair Pinellia ternate-Magnolia officinalis(BX-HP)in liver cancer through network pharmacology using molecular docking methods.Method:The active ingredients and corresponding targets of the herb pair Pinellia ternate-Magnolia officinalis were obtained from the HERB database.The relevant targets for liver cancer were obtained from GeneCards,DisGeNET,TTD,and Drugbank databases.Obtain common targets between herb pair Pinellia ternate-Magnolia officinalis and liver cancer through the Bioinformatics platform,establish a PPI network diagram using STRING software,and perform GO functional enrichment and KEGG pathway enrichment analysis on the DAVID platform.AutoDockTools 1.5.7 software and molecular dynamics simulation analysis are used to evaluate the binding of components to target proteins.HERB database,SwissTargetPrediction database,SwissADME database,UniProt database,GeneCards database,TTD database,DRUGBANK database,DisGeNET database,String,DAVID.Bioinformatics platform,PDB database,PubChem and TCMSP database.Result:A total of 22 active ingredients with a Probability>0.1 targets in Magnolia officinalis were screened,26 active ingredients with a Probability>0.1 targets in Pinellia ternata,ten vital active ingredients,corresponding to 979 and 803 targets with a Probability>0.1 targets,2536 liver cancer-related targets,and 279 targets in the herb pair Pinellia ternata-Magnolia officinalis.The GO functional enrichment analysis resulted in 1297 entries,namely 971 biological process entries,118 cell localization entries,and 208 molecular function entries.Three signaling pathways were annotated through the KEGG pathway.Based on molecular docking,ten vital active ingredients and five target proteins were validated to exhibit an excellent binding affinity.The above data indicates that combining the herb pair Pinellia ternata-Magnolia officinalis may treat liver cancer through specific targets and signaling pathways.Conclusion:Herb pair Pinellia ternata-Magnolia officinalis has a synergistic effect on treating liver cancer through multicomponent,multitarget,and multi-pathway approaches.This study provides a sufficient theoretical basis for subsequent research.

厚朴生姜半夏甘草人参汤加味联合艾灸治疗功能性消化不良的临床效果观察

目的观察厚朴生姜半夏甘草人参汤加味联合艾灸治疗功能性消化不良的有效性。方法将100例功能性消化不良患者随机分为对照组和观察组,每组50例。对照组予以艾灸治疗,观察组予以厚朴生姜半夏甘草人参汤加减联合艾灸治疗,比较2组临床治疗效果与治疗前后的临床症状评分变化。结果观察组治愈率、总有效率为78. 00%、98. 00%,高于对照组的34. 00%、52. 00%(P均<0. 01);治疗后,2组腕胁胀痛、嗳气、泛酸、胃烧灼感评分较治疗前均降低,且观察组各项症状评分均低于对照组(P <0. 01)。结论厚朴生姜半夏甘草人参汤加减联合艾灸能够有效提高功能性消化不良患者的治愈率,具有较高的临床推广价值。

厚朴生姜半夏甘草人参汤不同配伍对脾虚气滞型功能性消化不良的影响

目的:探讨厚朴生姜半夏甘草人参汤不同配伍对脾虚气滞型功能性消化不良的影响;方法:将40只KM小鼠随机分为5组,重用厚朴组、重用人参组、完全空白组、模型空白组、多潘立酮组,每组各8只。采用中药制造脾虚气滞功能性消化不良模型,造模成功后重用厚朴组、重用人参组给予厚朴生姜半夏甘草人参汤灌胃,模型空白组给予等量无菌水灌胃,完全空白组不给予药物灌胃,多潘立酮组给予多潘立酮灌胃。干预3d后检测小鼠血胃动素,计算小鼠胃残留率。结果:灌胃3周后,模型空白组、重用厚朴组、重用人参组、多潘立酮组体质量及进食量出现明显下降,与完全空白组比较,差异均有统计学意义(P<0.05)。小鼠胃残留率、血清胃动素方面,完全空白组与模型空白组比较,差异均有统计学意义(P<0.05);模型空白组与重用人参组比较,差异无统计学意义(P>0.05);重用厚朴组与重用人参组、多潘立酮组比较,差异均有统计学意义(P<0.05,P<0.01)。结论:对于脾虚气滞型功能性消化不良小鼠,重用厚朴疗效优于重用人参。