【目的】探明不同产地八角果实挥发性代谢物组分,为八角种质资源评价、优质原料选材提供参考。【方法】以云南(西畴县、广南县、马关县、麻栗坡县)和广西(崇左市、钦州市)八角果实为材料,采用全自动顶空固相微萃取(HS-SPME)和气质联用(G...【目的】探明不同产地八角果实挥发性代谢物组分,为八角种质资源评价、优质原料选材提供参考。【方法】以云南(西畴县、广南县、马关县、麻栗坡县)和广西(崇左市、钦州市)八角果实为材料,采用全自动顶空固相微萃取(HS-SPME)和气质联用(GC-MS)技术,广泛靶向检测各产地八角果实挥发性代谢物,并通过主成分和正交偏最小二乘法分析挥发性代谢物成分。【结果】八角果实挥发性代谢物含量以萜类、酯类和杂环化合物相对较高,分别占果实总挥发性代谢物的31%、14%和11%。不同产地八角果实挥发性代谢物成分存在明显差异,差异代谢物共有212~511个,其中,萜类物质含量最高,其次为杂环化合物和酮类物质。广南县和崇左市八角果实差异代谢物最多,为511个,其中,上调和下调具有代表性的10个差异代谢物中,酯类物质最多,其次是萜类和醇类。KEGG注释表明广南县八角果实单萜生物合成途径(Monoterpenoid biosynthesis)明显上调,倍半萜和三萜生物合成途径(Sesquiterpenoid and triterpenoid biosynthesis)、泛醌和其他萜类醌生物合成途径(Ubiquinone and other terpenoid-quinone biosynthesis)明显下调。【结论】八角果实挥发性代谢物主要为萜类、酯类和杂环化合物。6个不同产地八角果实挥发性代谢物呈现一定差异,差异代谢物以萜类为主,其次为杂环化合物和酮类物质,可作为评价和筛选香料和药用八角种质资源的依据。展开更多
A study was conducted to explore the mechanism that emissions of volatile organic compounds（VOC） from heated needles and twigs（200°C,within 15 min） of Pinus pumila affect fire behaviours using the technology ...A study was conducted to explore the mechanism that emissions of volatile organic compounds（VOC） from heated needles and twigs（200°C,within 15 min） of Pinus pumila affect fire behaviours using the technology of Thermal Desorption-Gas Chromatography-Mass Spectrometry（TD-GC-MS）.The results indicated that the main components of VOC from heated needles and twigs are terpenoids.Most of these terpenoids are monoterpenes.Terpenoids account for 72.93% for the needles and 92.40% for the twigs of the total VOC,and their emission ratios are 61.200 μg·g-1 and 217.060 μg·g-1 respectively.Heated twigs can emit more terpenoids than heated needles because twigs had more volatile oils than needles.In actual fires,these large amounts of terpenoid emissions,especially the monoterpene emissions,have strong effects on fire behaviors that are not only in the initial stage but also in the fast propagation stage of fires.These flammable gases are capable of causing violent combustion and creating crown fires.In addition,if these gases accumulate in an uneven geographical area,there will be a possible for eruptive fires and/or fires flashover to occur.展开更多
采用顶空固相微萃取结合全二维气相色谱-飞行时间质谱(headspace solid phase microextraction and comprehensive two-dimensional gas chromatography/time-of-flight mass spectrometry,HS-SPME-GC×GC-TOFMS)技术研究了典型芝...采用顶空固相微萃取结合全二维气相色谱-飞行时间质谱(headspace solid phase microextraction and comprehensive two-dimensional gas chromatography/time-of-flight mass spectrometry,HS-SPME-GC×GC-TOFMS)技术研究了典型芝麻香型白酒挥发性组分特征。分析了芝麻香型白酒挥发性组分在二维色谱系统中的分离规律,发现了化合物同系物规律性分布特征。采用该技术在景芝芝麻香型白酒中检测到1 029个色谱峰,进一步采用多级鉴定策略确认了可信度较高的挥发性化合物340种。在芝麻香型白酒中鉴定出的挥发性组分主要包括酯类130种,醇类26种,有机酸类15种,醛、酮、缩醛类88种,含氮化合物16种,呋喃类20种,含硫化合物25种,萜烯类14种,其他类6种。其中首次鉴定出挥发性含硫化合物11种和萜烯类化合物12种。多种挥发性含硫化合物香气独特,香气阈值极低可能对芝麻香型白酒香气特征具有重要贡献。芝麻香型白酒中鉴定出的萜烯类化合物除了具有独特的香气特征外,还具有重要的生理活性。展开更多
文摘【目的】探明不同产地八角果实挥发性代谢物组分,为八角种质资源评价、优质原料选材提供参考。【方法】以云南(西畴县、广南县、马关县、麻栗坡县)和广西(崇左市、钦州市)八角果实为材料,采用全自动顶空固相微萃取(HS-SPME)和气质联用(GC-MS)技术,广泛靶向检测各产地八角果实挥发性代谢物,并通过主成分和正交偏最小二乘法分析挥发性代谢物成分。【结果】八角果实挥发性代谢物含量以萜类、酯类和杂环化合物相对较高,分别占果实总挥发性代谢物的31%、14%和11%。不同产地八角果实挥发性代谢物成分存在明显差异,差异代谢物共有212~511个,其中,萜类物质含量最高,其次为杂环化合物和酮类物质。广南县和崇左市八角果实差异代谢物最多,为511个,其中,上调和下调具有代表性的10个差异代谢物中,酯类物质最多,其次是萜类和醇类。KEGG注释表明广南县八角果实单萜生物合成途径(Monoterpenoid biosynthesis)明显上调,倍半萜和三萜生物合成途径(Sesquiterpenoid and triterpenoid biosynthesis)、泛醌和其他萜类醌生物合成途径(Ubiquinone and other terpenoid-quinone biosynthesis)明显下调。【结论】八角果实挥发性代谢物主要为萜类、酯类和杂环化合物。6个不同产地八角果实挥发性代谢物呈现一定差异,差异代谢物以萜类为主,其次为杂环化合物和酮类物质,可作为评价和筛选香料和药用八角种质资源的依据。
基金supported by National Natural Science Foundation of China (30872037, 31070587)Open Project Program of State Key Laboratory of Fire Science, University of Science and Technology of China (HZ2008-KF08)
文摘A study was conducted to explore the mechanism that emissions of volatile organic compounds（VOC） from heated needles and twigs（200°C,within 15 min） of Pinus pumila affect fire behaviours using the technology of Thermal Desorption-Gas Chromatography-Mass Spectrometry（TD-GC-MS）.The results indicated that the main components of VOC from heated needles and twigs are terpenoids.Most of these terpenoids are monoterpenes.Terpenoids account for 72.93% for the needles and 92.40% for the twigs of the total VOC,and their emission ratios are 61.200 μg·g-1 and 217.060 μg·g-1 respectively.Heated twigs can emit more terpenoids than heated needles because twigs had more volatile oils than needles.In actual fires,these large amounts of terpenoid emissions,especially the monoterpene emissions,have strong effects on fire behaviors that are not only in the initial stage but also in the fast propagation stage of fires.These flammable gases are capable of causing violent combustion and creating crown fires.In addition,if these gases accumulate in an uneven geographical area,there will be a possible for eruptive fires and/or fires flashover to occur.
文摘采用顶空固相微萃取结合全二维气相色谱-飞行时间质谱(headspace solid phase microextraction and comprehensive two-dimensional gas chromatography/time-of-flight mass spectrometry,HS-SPME-GC×GC-TOFMS)技术研究了典型芝麻香型白酒挥发性组分特征。分析了芝麻香型白酒挥发性组分在二维色谱系统中的分离规律,发现了化合物同系物规律性分布特征。采用该技术在景芝芝麻香型白酒中检测到1 029个色谱峰,进一步采用多级鉴定策略确认了可信度较高的挥发性化合物340种。在芝麻香型白酒中鉴定出的挥发性组分主要包括酯类130种,醇类26种,有机酸类15种,醛、酮、缩醛类88种,含氮化合物16种,呋喃类20种,含硫化合物25种,萜烯类14种,其他类6种。其中首次鉴定出挥发性含硫化合物11种和萜烯类化合物12种。多种挥发性含硫化合物香气独特,香气阈值极低可能对芝麻香型白酒香气特征具有重要贡献。芝麻香型白酒中鉴定出的萜烯类化合物除了具有独特的香气特征外,还具有重要的生理活性。
