APOPTOSIS OF EPIDIDYMIS EPITHELIUM AND CONTENT OF EPIDIDYMIS SIALIC ACID FOLLOWING TORSED/DETORSED TESTES IN RAT

To investigate the apoptosis of epididymis epithelium and the change of epididymis sialic acid following torsed/detorsed testes. Methods Twenty four adult male Sprague-Dawley rats were subjected to unilateral 720 testicular torsion with the duration of 2h and 4h, then repaired. The ischemic epididymis were collected for detecting the content of sialic acid by using spectrophotometry and the apoptosis with TUNEL technique.Results There were no statistically significant difference in the apoptosis of epididymis epithelium [ ( 9. 51 +2.78) % vs (6. 34 + 1.98) % I and the content of epididymis sialic acid(23. 3851 + 9. 2199mg/mgprot vs 19. 36616. 3373mg/mgprot ) at 24h between following 2h-torsed/detorsed testes and those of sham group. There were statistically significant difference in the apopotosis of epididymis epithelium[ ( 46. 81 + 3.55 ) % vs ( 6. 34 + 1.98 ) % ] and the content of epididymis sialic acid ( 13. 7249 + 7. 8006mg/ mgprot vs 19. 3661 + 6. 3373mg/ mgprot ) at 24h between following 4h-torsed/detorsed testes and those of sham group (P <0. 05 ). Conclusion The results suggest that the sialic acid-secreting-function of epididymis remain normal at 24h following 2h-torsed/detorsed testes, while the apoptosis index of epididymis epithelium do not increase. The epididymis would be injured at 24h following 4htorsed/detorsed testes, while the apoptosis index increased.

大鼠睾丸扭转复位后附睾唾液酸含量变化及意义

目的 :探讨大鼠睾丸扭转 2h和 4h复位后 2 4h附睾唾液酸含量的变化和意义。 方法 :用 2 4只雄性SD大鼠建立左侧睾丸扭转复位模型 ,分为对照组、扭转 2h组和 4h组 ,每组 8只。 5 甲基苯二酚法检测扭转侧附睾唾液酸的含量。 结果 :睾丸扭转 2h复位后 2 4h扭转侧附睾唾液酸含量 [(2 3.385± 9.2 2 0 )mg/mgprot]改变不明显 ;睾丸扭转 4h复位后 2 4h附睾唾液酸含量 [(13.72 5± 7.80 1)mg/mgprot]下降明显 (P <0 .0 5 )。 结论 :睾丸扭转 2h复位后 2 4h附睾分泌唾液酸功能不受影响 ,扭转 4h复位后 2 4h附睾分泌唾液酸功能下降 ;附睾耐受缺血再灌注损伤的时间可能较长。

大鼠睾丸扭转复位后附睾上皮细胞凋亡及唾液酸变化

目的:探讨大鼠睾丸扭转复位后,附睾上皮细胞凋亡与唾液酸分泌的关系。方法:24只雄性SD大鼠建立左侧睾丸扭转复位模型,分为对照组、扭转2h和4h共3组,每组8只。TUNEL法检测附睾上皮细胞凋亡,5-甲基苯二酚法检测扭转侧附睾唾液酸的含量。结果:睾丸扭转2h复位后24h,扭转侧附睾上皮细胞凋亡指数与对照组相比上升不明显(P>0.05),唾液酸含量改变不明显(P>0.05);扭转4h复位后24h,扭转侧附睾上皮细胞凋亡指数与对照组相比上升极显著(P<0.001),唾液酸含量下降明显(P<0.05)。结论:睾丸扭转2h复位后24h,附睾上皮细胞未发生凋亡,其分泌唾液酸功能不受影响;扭转4h复位后24h,附睾上皮细胞凋亡严重,其分泌唾液酸功能下降。