The acute toxicity of 2,2′,4,4′-tetrabromodiphenyl ether on Chlorella vulgaris, Dqohnia magna and Barchydanio rerio was analyzed using semi-static water contacting acute toxicity method. The results showed that the ...The acute toxicity of 2,2′,4,4′-tetrabromodiphenyl ether on Chlorella vulgaris, Dqohnia magna and Barchydanio rerio was analyzed using semi-static water contacting acute toxicity method. The results showed that the 96 h-EC50 of 2,2′,4,4′-tetrabromodiphenyl ether(BDE-47) on C. vulgaris was 3.97 μg/L, the 48 h-LC50 and 96 h-LC50 of BDE-47 on D. magna were 1.09 and 0.84 mg/L respectively, and the 96 h-LC50 of BDE-47 on B. rerio was higher than 56.2 mg/L. According to the grading standard of toxicity evaluation, BDE-47 had extremely high toxicity to C. vulgaris, high toxicity to D. magna, and low to medium toxicity to B. rerio. The toxicity of BDE-47 to C. vulgaris, D. magna and B. rerio followed the order of C. vulgaris>D. magna>B. rerio. For the water solubility of BDE-47 is extremely low(15 μg/L), it could not cause the acute poisoning death of D. magna and B. rerio.展开更多
Polybrominated diphenyl ethers （PBDEs） are ubiquitous global pollutants, which are known to have immune, development, reproduction, and endocrine toxicity in aquatic organisms, including bivalves. 2,2＇,4,4＇-Tetrab...Polybrominated diphenyl ethers （PBDEs） are ubiquitous global pollutants, which are known to have immune, development, reproduction, and endocrine toxicity in aquatic organisms, including bivalves. 2,2＇,4,4＇-Tetrabromodiphenyl ether （BDE-47） is the predominant PBDE congener detected in environmental samples and the tissues of organisms. However, the mechanism of its toxicity remains unclear. In this study, high-throughput sequencing was performed using the clam Mactra veneriformis, a good model for toxicological research, to clarify the transcriptomic response to BDE-47 and the mechanism responsible for the toxicity of BDE-47. The clams were exposed to 5 pg/L BDE-47 for 3 days and the digestive glands were sampled for high-throughput sequencing analysis. We obtained 127 648, 154 225, and 124 985 unigenes by de novo assembly of the control group reads （CG）, BDE-47 group reads （BDEG）, and control and BDE-47 reads （CG ＆ BDEG）, respectively. We annotated 32 176 unigenes from the CG ＆ BDEG reads using the NR database. We categorized 24 401 unigenes into 25 functional COG clusters and 21 749 unigenes were assigned to 259 KEGG pathways. Moreover, 17 625 differentially expressed genes （DEGs） were detected, with 10 028 upregulated DEGs and 7 597 downregulated DEGs. Functional enrichment analysis showed that the DEGs were involved with detoxification, antioxidant defense, immune response, apoptosis, and other functions. The mRNA expression levels of 26 DEGs were verified by quantitative real-time PCR, which demonstrated the high agreement between the two methods. These results provide a good basis for future research using the M. veneriformis model into the mechanism of PBDEs toxicity and molecular biomarkers for BDE-47 pollution. The regulation and interaction of the DEGs would be studied in the future for clarifying the mechanism of PBDEs toxicity.展开更多
基金Supported by Central Public-interest Scientific Institution Basal Research Fund,Chinese Academy of Fishery Science(2017HY-ZD0208)China Agricultural Research System-Freshwater Fish(CARS-46)
文摘The acute toxicity of 2,2′,4,4′-tetrabromodiphenyl ether on Chlorella vulgaris, Dqohnia magna and Barchydanio rerio was analyzed using semi-static water contacting acute toxicity method. The results showed that the 96 h-EC50 of 2,2′,4,4′-tetrabromodiphenyl ether(BDE-47) on C. vulgaris was 3.97 μg/L, the 48 h-LC50 and 96 h-LC50 of BDE-47 on D. magna were 1.09 and 0.84 mg/L respectively, and the 96 h-LC50 of BDE-47 on B. rerio was higher than 56.2 mg/L. According to the grading standard of toxicity evaluation, BDE-47 had extremely high toxicity to C. vulgaris, high toxicity to D. magna, and low to medium toxicity to B. rerio. The toxicity of BDE-47 to C. vulgaris, D. magna and B. rerio followed the order of C. vulgaris>D. magna>B. rerio. For the water solubility of BDE-47 is extremely low(15 μg/L), it could not cause the acute poisoning death of D. magna and B. rerio.
基金Supported by the National Natural Science Foundation of China(No.41206120)the Development Plan of Science and Technology in Yantai(No.2011062)+1 种基金the National Special Research Fund for Non-Profit Marine Sector(No.201205023)the Ludong University Research Funding(No.210-32040301)
文摘Polybrominated diphenyl ethers （PBDEs） are ubiquitous global pollutants, which are known to have immune, development, reproduction, and endocrine toxicity in aquatic organisms, including bivalves. 2,2＇,4,4＇-Tetrabromodiphenyl ether （BDE-47） is the predominant PBDE congener detected in environmental samples and the tissues of organisms. However, the mechanism of its toxicity remains unclear. In this study, high-throughput sequencing was performed using the clam Mactra veneriformis, a good model for toxicological research, to clarify the transcriptomic response to BDE-47 and the mechanism responsible for the toxicity of BDE-47. The clams were exposed to 5 pg/L BDE-47 for 3 days and the digestive glands were sampled for high-throughput sequencing analysis. We obtained 127 648, 154 225, and 124 985 unigenes by de novo assembly of the control group reads （CG）, BDE-47 group reads （BDEG）, and control and BDE-47 reads （CG ＆ BDEG）, respectively. We annotated 32 176 unigenes from the CG ＆ BDEG reads using the NR database. We categorized 24 401 unigenes into 25 functional COG clusters and 21 749 unigenes were assigned to 259 KEGG pathways. Moreover, 17 625 differentially expressed genes （DEGs） were detected, with 10 028 upregulated DEGs and 7 597 downregulated DEGs. Functional enrichment analysis showed that the DEGs were involved with detoxification, antioxidant defense, immune response, apoptosis, and other functions. The mRNA expression levels of 26 DEGs were verified by quantitative real-time PCR, which demonstrated the high agreement between the two methods. These results provide a good basis for future research using the M. veneriformis model into the mechanism of PBDEs toxicity and molecular biomarkers for BDE-47 pollution. The regulation and interaction of the DEGs would be studied in the future for clarifying the mechanism of PBDEs toxicity.
