Objective: To investigate the effects of perorally administered juice on tetrachloromethane(CCl4)-induced hepatotoxicity model in rats. Methods: Male Wistar rats were tubeadministrated silymarin, Ecballium juice at 0....Objective: To investigate the effects of perorally administered juice on tetrachloromethane(CCl4)-induced hepatotoxicity model in rats. Methods: Male Wistar rats were tubeadministrated silymarin, Ecballium juice at 0.2 mL/kg and 0.7 mL/kg daily for 3 consequent days, ie. 3.28 μg and 11.48 μg of cucurbitacin B per kg of body weight respectively. On the third day, liver damage was induced by intraperitoneal application of CCl4. On the fourth day, abdominal cavity was macroscopically examined and liver samples were taken for histopathological and immunochemical evaluation. HPLC was used to determine the content of the active substance cucurbitacin B. Results: The experiment revealed that 0.7 ml/kg juice concentration expressed the highest pro-apoptotic activity, but with prevailing negative effects. Compared with the lower concentration, there was an observable vasodilatation with consequent interstitial hemorrhages and a larger scope of inflammatory damage, which suppressed the hepatoprotective effect. In the 0.2 mL/kg concentration, there was a smaller pro-apoptotic activity but other parameters had better results, and the liver parenchyma damage was reversible. Conclusions: No reactions confirming the potentially allergic effect on laboratory rats were observed; its hepatoprotective and anti-inflammatory effect was confirmed on a model of acute liver damage.展开更多
We have reported the solid and liquid phase diagrams for tetrachloromethane+cyclopentanone, +4-butyrolactone, and +1-methyl-2-pyrrolidinone.The solid compounds are formed in all the three systems. The second component...We have reported the solid and liquid phase diagrams for tetrachloromethane+cyclopentanone, +4-butyrolactone, and +1-methyl-2-pyrrolidinone.The solid compounds are formed in all the three systems. The second component in the three tetrachloromethane mixtures have cyclic structure. Ref. [2] pointed out that the cyclic structure of the second component is very important during the compound formation in展开更多
文摘Objective: To investigate the effects of perorally administered juice on tetrachloromethane(CCl4)-induced hepatotoxicity model in rats. Methods: Male Wistar rats were tubeadministrated silymarin, Ecballium juice at 0.2 mL/kg and 0.7 mL/kg daily for 3 consequent days, ie. 3.28 μg and 11.48 μg of cucurbitacin B per kg of body weight respectively. On the third day, liver damage was induced by intraperitoneal application of CCl4. On the fourth day, abdominal cavity was macroscopically examined and liver samples were taken for histopathological and immunochemical evaluation. HPLC was used to determine the content of the active substance cucurbitacin B. Results: The experiment revealed that 0.7 ml/kg juice concentration expressed the highest pro-apoptotic activity, but with prevailing negative effects. Compared with the lower concentration, there was an observable vasodilatation with consequent interstitial hemorrhages and a larger scope of inflammatory damage, which suppressed the hepatoprotective effect. In the 0.2 mL/kg concentration, there was a smaller pro-apoptotic activity but other parameters had better results, and the liver parenchyma damage was reversible. Conclusions: No reactions confirming the potentially allergic effect on laboratory rats were observed; its hepatoprotective and anti-inflammatory effect was confirmed on a model of acute liver damage.
基金Project supported by the National Natural Science Foundation of China
文摘We have reported the solid and liquid phase diagrams for tetrachloromethane+cyclopentanone, +4-butyrolactone, and +1-methyl-2-pyrrolidinone.The solid compounds are formed in all the three systems. The second component in the three tetrachloromethane mixtures have cyclic structure. Ref. [2] pointed out that the cyclic structure of the second component is very important during the compound formation in
