In this work,a novel composite material based on β-cyclodextrin-immobilized sodium alginate aerogel(β-CD/NaAlg) was developed utilizing cross-linker of epichlorohydrin and applied as an adsorbent to remove tetracycl...In this work,a novel composite material based on β-cyclodextrin-immobilized sodium alginate aerogel(β-CD/NaAlg) was developed utilizing cross-linker of epichlorohydrin and applied as an adsorbent to remove tetracycline antibiotics from reclaimed wastewater.A series of characterizations were utilized to confirm the successful synthesis of the adsorbent and this β-CD/NaAlg presented a three-dimensional network at the nanoscale or microscale.Under optimal conditions(pH=4,t=8 h,β-CD:NaAlg=9,adsorbent dosage = 1.5 g·L-1),the maximum removal rate of β-CD/NaAlg to tetracycline was 70%.The adsorption behavior of tetracycline on β-CD/NaAlg conformed to the Freundlich isotherm model(R2=0.9977) and the pseudo-second-order kinetic model(R^(2)=0.9993).Moreover,the adsorbent still removed 55.3% of tetracycline after five cycles.Specially,the adsorbent was integrated with ultrafiltration to adsorb tetracycline antibiotics from simulated reclaimed wastewater,and the removal rate of tetracycline reached 78.9% within 2 h.The existence of Cr(Ⅵ) had a negligible impact on tetracycline removal,while the presence of humic acid exhibited a promoting effect.The possible adsorption mechanisms were also elucidated through X-ray photoelectron spectroscopy and density functional theory analysis.In summary,β-CD/NaAlg represents an environmentally friendly,efficient,and sustainable adsorbent for removing tetracycline antibiotics from reclaimed water.展开更多
Tetracycline and analogues are among the most used antibiotics in the dairy industry. Besides the therapeutic uses, tetracyclines are often incorporated into livestock feed as growth promoters. A considerable amount o...Tetracycline and analogues are among the most used antibiotics in the dairy industry. Besides the therapeutic uses, tetracyclines are often incorporated into livestock feed as growth promoters. A considerable amount of antibiotics is released unaltered through milk from dairy animals. The presence of antibiotic residues in milk and their subsequent consumption can lead to potential health impacts, including cancer, hypersensitivity reactions, and the development of antibiotic resistance. Thus, it is important to monitor residual levels of tetracyclines in milk. The purpose of this study is to develop a quick and simple method for simultaneously extracting five tetracycline analogues from bovine milk. Specifically, five tetracycline analogues: Chlortetracycline (CTC), demeclocycline (DEM), doxycycline (DC), minocycline (MC), and tetracycline (TC) were simultaneously extracted from milk using trifluoroacetic acid. Subsequently, the extracted analogues were separated by reverse-phase high-performance liquid chromatography (RP-HPLC) and detected at 355 nm using UV/Vis. Calibration curves for all five tetracycline analogues show excellent linearity (r2 value > 0.99). Percent recovery for MC, TC, DEM, CTC, and DC were: 31.88%, 96.91%, 151.29, 99.20%, and 85.58% respectively. The developed extraction method has good precision (RSD < 9.9% for 4 of the 5 analogues). The developed method with minimal sample preparation and pretreatment has the potential to serve as an initial screening test.展开更多
AIM: To investigate the resistance of Helicobacter pylori (H. pylori ) to 6 commonly used antibiotics from 2000 to 2009 in Shanghai. METHODS: A total of 293 H. pylori strains were collected from 2000 to 2009 in Shangh...AIM: To investigate the resistance of Helicobacter pylori (H. pylori ) to 6 commonly used antibiotics from 2000 to 2009 in Shanghai. METHODS: A total of 293 H. pylori strains were collected from 2000 to 2009 in Shanghai and tested for their susceptibility to metronidazole, clarithromycin, amoxicillin, furazolidone, levofloxacin and tetracycline using agar dilution. RESULTS: The resistant rates of H. pylori to clarithromycin (8.6%, 9.0% and 20.7%) and levofloxacin (10.3%, 24.0% and 32.5%) increased from 2000 to 2009 in Shanghai. The resistant rate of H. pylori to metronidazole remained stable (40%-50%). Only one strain of H. pylori isolated in 2005 was resistant to tetracycline. All strains were sensitive to amoxicillin and furazolidone.The resistant rate of H. pylori to antibiotics was not related with the sex, age and clinical outcome of patients. CONCLUSION: Resistance of H. pylori to antibiotics plays an important role in making treatment strategies against H. pylori -associated diseases.展开更多
High selective molecularly imprinted polymers （MIPs） for tetracycline have been prepared by precipitation polymerization. Effects of monomer and solvent, the ratio of monomer and template and the characterization of...High selective molecularly imprinted polymers （MIPs） for tetracycline have been prepared by precipitation polymerization. Effects of monomer and solvent, the ratio of monomer and template and the characterization of the polymer were investigated by frontal chromatography and selectivity experiment. The results clearly indicated that the polymer, which had the highest molecular recognition abilities for tetracycline antibiotics, had been received.展开更多
We tested the utility of chemical marking techniques in the juvenile black rockfish Sebastes schlegelii. Juveniles （30-40 mm total length） were immersed in a range of tetracycline hydrochloride （TC） solutions at c...We tested the utility of chemical marking techniques in the juvenile black rockfish Sebastes schlegelii. Juveniles （30-40 mm total length） were immersed in a range of tetracycline hydrochloride （TC） solutions at concentrations ranging from 300 to 500 mg/L, and alizarin complexone （ALC） solutions at concentrations ranging from 200 to 400 mg/L in filtered sea water （salinity of 30） for 24 h, respectively. Otoliths （sagittae, asteriscus）, scales, fin rays （dorsal, pectoral, ventral, anal, and caudal fin rays）, and fin spines （dorsal, ventral, and anal fin spines） were sampled and used to detect fluorescent marks after a 60-day growth experiment. With the exception of 300 mg/L TC, acceptable marks were produced in the otoliths and fin spines by all concentrations of TC and ALC. In particular, we observed clearly visible marks in the sagittae, asteriscus, and fin spines under normal light at concentrations of200~00 mg/L, 250-400 mg/L, and 250-400 mg/L ALC, respectively. Scales and fin rays had acceptable marks at much higher concentrations （_〉350 mg/L TC, 〉250 mg/L ALC for scales and _〉350 mg/L TC, 〉300 mg/L ALC for fin rays）. The best mark quality （i.e., acceptable marks were observed in all sampled structures after immersion marking） were obtained following immersion in TC at between 350-500 rag/L, and ALC between 300-400 mg/L. In addition, there was no significant difference in survival and growth of TC and ALC marked fish compared to their controls up to 60 days post-marking （P〉0.05）.展开更多
In recent years,the excessive use of antibiotics has become a serious problem for human health.BiV04 regarded as one of the most promising visible-light-driven photocatalysts was used to degrade the antibiotics.In thi...In recent years,the excessive use of antibiotics has become a serious problem for human health.BiV04 regarded as one of the most promising visible-light-driven photocatalysts was used to degrade the antibiotics.In this paper,we fabricated Bi/BiV04 plasmonic photocatalysts which enhanced the photocatalytic activity of BiV04 for degradation of tetracycline(TC)antibiotic.The Bi/BiV04 photocatalysts were characterized by X-ray diffraction,X-ray photoelectron spectroscopy,scanning electron microscopy,transmission electron microscopy and high-resolution transmission electron microscopy.In addition,the photocatalytic experiment results show that the 0.04-Bi/BiVO4 sample has the best photocatalytic activity for 2 times than the pure BiV04 photocatalyst.The cycle experiments,after four repetitions of the experiments,showed the sample still maintained a high photocatalytic activity.Finally,the photocatalytic reaction mechanism was also studied by free radical capture experiments and electron paramagnetic resonance spectroscopy.展开更多
Oxytetracycline(OTC) and tetracycline(TC) are the most common TC antibiotics used in human and veterinary medicine.Residual amounts of these antibiotics in manure pose a potential threat to public and ecological healt...Oxytetracycline(OTC) and tetracycline(TC) are the most common TC antibiotics used in human and veterinary medicine.Residual amounts of these antibiotics in manure pose a potential threat to public and ecological health as a result of the potential for them to be released to the environment following land application of manure from animals treated with antibiotics.We investigated the degradation of OTC and TC during anaerobic composting.We tested the effects of temperature and antibiotic concentration on degradation rates in a control and in manure spiked with TCs.We examined changes in p H,biological degradation material(BDM),and moisture corresponding with antibiotic degradation of TCs in the swine manure.Results showed that the OTC and TC concentrations decreased by between 68.54% and 95.50% in all nine treatments following 14 days of anaerobic composting,and the highest removal ef ficiencies were observed at an incubation temperature of 55°C and initial concentrations of 10.36 μg·g^(-1),and 5.96 μg·g^(-1) of OTC and TC,respectively,which were degraded by 95.50%,and 90.06%.During composting at 55 °C and at added concentrations of 5 μg·g^(-1),OTC decreased rapidly,and the time required for50% and 90% degradation was 4.1 and 9.8 days,respectively;for TC,these values were 4.4 and 14.0 days,respectively.Removal ef ficiencies for all TCs correlated well with moisture content of the manure.These results show that composting may be a practical and useful means to reduce concentrations of OTC and TC in swine manure prior to its land application.展开更多
Recently increasing concerns from the scientists and public have been paid for seawater pollution due to tetracycline(TC)overuse in maricultural area.However,there are few methods or instruments that can be used for s...Recently increasing concerns from the scientists and public have been paid for seawater pollution due to tetracycline(TC)overuse in maricultural area.However,there are few methods or instruments that can be used for specific and rapid detection of this antibiotic in seawater.In this study,the colloidal gold immunochromatographic assay(CG-ICA)was used to achieve this goal.A commercialized monoclonal antibody against TC(anti-TC mAb)was selected because of its higher sensitivity(half-maximal inhibitory concentration of 2.38μgL^(-1)).The prepared CG particles(average diameter of 20 nm)were used to label anti-TC mAb at pH 8.0.The conjugate pad was formed by spraying the CG-labeled anti-TC mAb on a glass fibre membrane followed by proper dryness.The test pad was made by immobilizing artificial antigen and anti-mouse mAb in the test line and the control line,respectively,in a nitrocellulose membrane.The test strip,assembled with sample pad,conjugate pad,test pad and absorbent pad,could be used to detect TC during seawater sample flowing through these components in turn.The results could be observed by the naked eye in 10min.The visible limit of detection(vLOD)was 20μgL^(-1) for TC in seawater.The CG-ICA test results were in good agreement with those of liquid chromatography-tandem mass spectrometry(LC-MS/MS).The assay also showed that,oxytetracycline(OTC)and chlortetracycline(CTC),as the structural analogues of TC,did not interfere with TC determination.Furthermore,the TC concentration given by test strip could not be affected by the fluctuation of temperature(10℃–30℃),pH(7–9)and salinity(0–40)of seawater.Therefore,CG-ICA is a suitable tool for rapid,on-site,and semi-quantitative detection of TC in seawater.展开更多
The presence of tetracyclines in soil and surface water is an emerging concern. The present study was undertaken to investigate remediation of tetracylines (tetracycline (TC), oxytetracycline (OTC) and chlortetracycli...The presence of tetracyclines in soil and surface water is an emerging concern. The present study was undertaken to investigate remediation of tetracylines (tetracycline (TC), oxytetracycline (OTC) and chlortetracycline (CTC)) from aqueous solution using vetiver grass, water lettuce, and sunflower and root exudates of water lettuce, sunflower and from soil by tomato, Indian mustard and carrot plant. The data of this study denote that vetiver grass, water lettuce, sunflower remedy tetracyclines from water. The remediation % after 63 days of treatment was 87-61 for TC;88-68 for OTC and 87-68 for CTC. The remediation of tetracyclines at lower concentration of antibiotics in presence of root exudates of water lettuce and sunflower was more than 99% and remediation was faster than water lettuce or sunflower. The remediation of tetracyclines from aqueous solution may be due to oxidation of-OH group(s) of tetracycline through a process that is thought to involve reactive oxygen intermediates and/or role of peroxidase enzyme. The plant crops viz., tomato, Indian mustard and carrot can remedy 41%-72% of amended tetracyclines. The maximum bioaccumulation of TC and CTC was in Indian mustard and OTC was maximally bioaccumulated in tomato.展开更多
A coaxial dielectric barrier discharge (DBD) reactor was used for plasma-catalytic degradation of tetracycline hydrochloride over a series of Mn/γ-Al2O3 catalysts prepared by the incipient wetness impregnation method...A coaxial dielectric barrier discharge (DBD) reactor was used for plasma-catalytic degradation of tetracycline hydrochloride over a series of Mn/γ-Al2O3 catalysts prepared by the incipient wetness impregnation method.The combination of plasma and the Mn/γ-Al2O3 catalysts significantly enhanced the degradation efficiency of tetracycline hydrochloride compared to the plasma process alone,with the 10% Mn/γ-Al2O3 catalyst exhibiting the best tetracycline hydrochloride degradation efficiency.A maximum degradation efficiency of 99.3% can be achieved after 5 min oxidation and a discharge power of 1.3 W,with only 69.7% by a single plasma process.The highest energy yield of the plasma-catalytic process is 91.7 g kWh-1.Probable reaction mechanisms of the plasma-catalytic removal of tetracycline hydrochloride were also proposed.展开更多
Honey is being used as a pure natural and as an ingredient in many foods, pharmaceuticals and cosmetics than ever before, so honey testing has become essential to maintain its healthful characteristics and protect pub...Honey is being used as a pure natural and as an ingredient in many foods, pharmaceuticals and cosmetics than ever before, so honey testing has become essential to maintain its healthful characteristics and protect public health. Tetracyclines （TCs） are used for the treatment or prevention of American and European foul broad in bee colonies which are caused especially by two species of bacteria--Paenibacillus larvae and Melissococcus pluton. The present study aimed to determine the tetracycline residues in different types of local and imported honey. The reversed-phase high performance liquid chromatography （HPLC） with ultraviolet detection （UV） was used. Tetracycline and oxytetracycline were extracted from honey with 0.01 M sodium succinate buffer. Of the examined samples, 31.25% and 12.50% had these oxytetracycline and tetracycline residues, respectively. It was observed that all the samples studied have been reported residues of tetracycline and oxytetracycline more than international standards of maximum residue limit （MRLs）.展开更多
The fluorescence of tetracycline was greatly enhanced by chelation with Eu 3+ in Tris HCl buffer (pH8.5). The addition of cetyltrimethylammonium bromide, a cationic surfactant, further enhanced the fluorescence ...The fluorescence of tetracycline was greatly enhanced by chelation with Eu 3+ in Tris HCl buffer (pH8.5). The addition of cetyltrimethylammonium bromide, a cationic surfactant, further enhanced the fluorescence of tetracycline Eu 3+ chelates.This spectrofluorimetric method for the determination of tetracycline yielded a linear responeses in the range of 10 nmol/L 10 μmol/L. The detection limit was 5 nmol/L (or 2.22 ng/mL).The recovery of tetracycline spiked in serum at levels of l and 0.09 μg/mL was 97.9% and 97.8%, respectively. This method is fast, sensitive and suitable for the determination of tetracycline in biological specimens.展开更多
The Eu-Tetracycline(TC)-TOPO-sodium dodecyl sulfonate(SDS)system was studied,Experiments showed that the maximum fluorescence intensity was obtained in the pH range of 7.5-8.7 and the concentrations of Eu^(a+),TOPO an...The Eu-Tetracycline(TC)-TOPO-sodium dodecyl sulfonate(SDS)system was studied,Experiments showed that the maximum fluorescence intensity was obtained in the pH range of 7.5-8.7 and the concentrations of Eu^(a+),TOPO and SDS are 1.0×10^(-6)mol/L,1.0×10^(-8)mol/L and 1.0×10^(-8)mol/L,respectively,This fluorescence system can be used for the determination of TC in serum and urine,Beer's Law is obeyed in the range of 2.0×10^(-8)mol/L=1.0×10^(-6)mol/L for the concentration of TC. The determination limit is 1.2×10^(-8)mol/L,The composition and the luminescence mechanism were discussed.展开更多
[Objective]The aim is to provide data support for the prevention and control of pollution of exogenous chemical in pig breeding industry.[Methods]Solid-phase extraction（SPE）and tandem mass（LC-MS/MS）were used for d...[Objective]The aim is to provide data support for the prevention and control of pollution of exogenous chemical in pig breeding industry.[Methods]Solid-phase extraction（SPE）and tandem mass（LC-MS/MS）were used for determining four classes（tetracyclines,quinolones,macrolides and sulfonamides）of 10 common veterinary antibiotics in groundwater of an important pig breeding base in the Yangtze River Delta region of China.[Results]Results revealed the total concentration of the ten antibiotics in urban rivers ranged from 20.1 ng /L to 61.2 ng /L.The highest proportion was taken by tetracyclines,account for 95%,the concentration was up to 44.0 ng /L.Quinolones shared the second largest proportion of total concentration,about 29%,the concentration was up to 21.6 ng /L,while concentration of sulfonamides and macrolides were respectively below 2.7 ng /L and 6.3 ng /L.The highest total concentration of the ten antibiotics in rural rivers was up to 467 ng /L,60% of which was shared by tetracyclines,the highest concentration of tetracyclines was 253 ng /L.Sulfonamides share 20% of the total concentration,the highest concentration of it was 165 ng /L.The highest concentration of macrolides and quinolones was 14.6 ng /L and 14.5 ng /L,respectively.[Conclusion] Antibiotics＇ pollution in town rivers is more serious than urban rivers＇ pollution caused by antibiotics.展开更多
基金supported by the National Key Research and Development Program of China(2022YFC3801101)National Natural Science Foundation of China(52170028)+1 种基金the State Key Laboratory of Urban Water Resource and Environment,Harbin Institute of Technology(2023DX11)National Engineering Research Center for Safe Sludge Disposal and Resource Recovery(2021A003).
文摘In this work,a novel composite material based on β-cyclodextrin-immobilized sodium alginate aerogel(β-CD/NaAlg) was developed utilizing cross-linker of epichlorohydrin and applied as an adsorbent to remove tetracycline antibiotics from reclaimed wastewater.A series of characterizations were utilized to confirm the successful synthesis of the adsorbent and this β-CD/NaAlg presented a three-dimensional network at the nanoscale or microscale.Under optimal conditions(pH=4,t=8 h,β-CD:NaAlg=9,adsorbent dosage = 1.5 g·L-1),the maximum removal rate of β-CD/NaAlg to tetracycline was 70%.The adsorption behavior of tetracycline on β-CD/NaAlg conformed to the Freundlich isotherm model(R2=0.9977) and the pseudo-second-order kinetic model(R^(2)=0.9993).Moreover,the adsorbent still removed 55.3% of tetracycline after five cycles.Specially,the adsorbent was integrated with ultrafiltration to adsorb tetracycline antibiotics from simulated reclaimed wastewater,and the removal rate of tetracycline reached 78.9% within 2 h.The existence of Cr(Ⅵ) had a negligible impact on tetracycline removal,while the presence of humic acid exhibited a promoting effect.The possible adsorption mechanisms were also elucidated through X-ray photoelectron spectroscopy and density functional theory analysis.In summary,β-CD/NaAlg represents an environmentally friendly,efficient,and sustainable adsorbent for removing tetracycline antibiotics from reclaimed water.
文摘Tetracycline and analogues are among the most used antibiotics in the dairy industry. Besides the therapeutic uses, tetracyclines are often incorporated into livestock feed as growth promoters. A considerable amount of antibiotics is released unaltered through milk from dairy animals. The presence of antibiotic residues in milk and their subsequent consumption can lead to potential health impacts, including cancer, hypersensitivity reactions, and the development of antibiotic resistance. Thus, it is important to monitor residual levels of tetracyclines in milk. The purpose of this study is to develop a quick and simple method for simultaneously extracting five tetracycline analogues from bovine milk. Specifically, five tetracycline analogues: Chlortetracycline (CTC), demeclocycline (DEM), doxycycline (DC), minocycline (MC), and tetracycline (TC) were simultaneously extracted from milk using trifluoroacetic acid. Subsequently, the extracted analogues were separated by reverse-phase high-performance liquid chromatography (RP-HPLC) and detected at 355 nm using UV/Vis. Calibration curves for all five tetracycline analogues show excellent linearity (r2 value > 0.99). Percent recovery for MC, TC, DEM, CTC, and DC were: 31.88%, 96.91%, 151.29, 99.20%, and 85.58% respectively. The developed extraction method has good precision (RSD < 9.9% for 4 of the 5 analogues). The developed method with minimal sample preparation and pretreatment has the potential to serve as an initial screening test.
基金Supported by National Natural Science Foundation of China,No. 30770112Shanghai Leading Academic Discipline Project, No. Y0205
文摘AIM: To investigate the resistance of Helicobacter pylori (H. pylori ) to 6 commonly used antibiotics from 2000 to 2009 in Shanghai. METHODS: A total of 293 H. pylori strains were collected from 2000 to 2009 in Shanghai and tested for their susceptibility to metronidazole, clarithromycin, amoxicillin, furazolidone, levofloxacin and tetracycline using agar dilution. RESULTS: The resistant rates of H. pylori to clarithromycin (8.6%, 9.0% and 20.7%) and levofloxacin (10.3%, 24.0% and 32.5%) increased from 2000 to 2009 in Shanghai. The resistant rate of H. pylori to metronidazole remained stable (40%-50%). Only one strain of H. pylori isolated in 2005 was resistant to tetracycline. All strains were sensitive to amoxicillin and furazolidone.The resistant rate of H. pylori to antibiotics was not related with the sex, age and clinical outcome of patients. CONCLUSION: Resistance of H. pylori to antibiotics plays an important role in making treatment strategies against H. pylori -associated diseases.
文摘High selective molecularly imprinted polymers （MIPs） for tetracycline have been prepared by precipitation polymerization. Effects of monomer and solvent, the ratio of monomer and template and the characterization of the polymer were investigated by frontal chromatography and selectivity experiment. The results clearly indicated that the polymer, which had the highest molecular recognition abilities for tetracycline antibiotics, had been received.
基金Supported by the Special Fund for Agro-Scientific Research in the Public Interest(No.201003068)the National Natural Science Foundation of China(Nos.31172447,41176117)
文摘We tested the utility of chemical marking techniques in the juvenile black rockfish Sebastes schlegelii. Juveniles （30-40 mm total length） were immersed in a range of tetracycline hydrochloride （TC） solutions at concentrations ranging from 300 to 500 mg/L, and alizarin complexone （ALC） solutions at concentrations ranging from 200 to 400 mg/L in filtered sea water （salinity of 30） for 24 h, respectively. Otoliths （sagittae, asteriscus）, scales, fin rays （dorsal, pectoral, ventral, anal, and caudal fin rays）, and fin spines （dorsal, ventral, and anal fin spines） were sampled and used to detect fluorescent marks after a 60-day growth experiment. With the exception of 300 mg/L TC, acceptable marks were produced in the otoliths and fin spines by all concentrations of TC and ALC. In particular, we observed clearly visible marks in the sagittae, asteriscus, and fin spines under normal light at concentrations of200~00 mg/L, 250-400 mg/L, and 250-400 mg/L ALC, respectively. Scales and fin rays had acceptable marks at much higher concentrations （_〉350 mg/L TC, 〉250 mg/L ALC for scales and _〉350 mg/L TC, 〉300 mg/L ALC for fin rays）. The best mark quality （i.e., acceptable marks were observed in all sampled structures after immersion marking） were obtained following immersion in TC at between 350-500 rag/L, and ALC between 300-400 mg/L. In addition, there was no significant difference in survival and growth of TC and ALC marked fish compared to their controls up to 60 days post-marking （P〉0.05）.
基金Supported by the National Natural Science Foundation of China(21522603,21503142,21671083)Six Talent Peaks Project in Jiangsu Province(XCL-025)+1 种基金the China Postdoctoral Science Foundation(2017M611720)the Excellent Youth Foundation of Jiangsu Scientific Committee(BK20170526).
文摘In recent years,the excessive use of antibiotics has become a serious problem for human health.BiV04 regarded as one of the most promising visible-light-driven photocatalysts was used to degrade the antibiotics.In this paper,we fabricated Bi/BiV04 plasmonic photocatalysts which enhanced the photocatalytic activity of BiV04 for degradation of tetracycline(TC)antibiotic.The Bi/BiV04 photocatalysts were characterized by X-ray diffraction,X-ray photoelectron spectroscopy,scanning electron microscopy,transmission electron microscopy and high-resolution transmission electron microscopy.In addition,the photocatalytic experiment results show that the 0.04-Bi/BiVO4 sample has the best photocatalytic activity for 2 times than the pure BiV04 photocatalyst.The cycle experiments,after four repetitions of the experiments,showed the sample still maintained a high photocatalytic activity.Finally,the photocatalytic reaction mechanism was also studied by free radical capture experiments and electron paramagnetic resonance spectroscopy.
基金Supported by the Innovative Team Foundation of Zhejiang Province(2013TD12)the International Science and Technology Cooperation Program of China(2014DFE90040)the Significant Science and Technology Project of Zhejiang Province(2015C33006)
文摘Oxytetracycline(OTC) and tetracycline(TC) are the most common TC antibiotics used in human and veterinary medicine.Residual amounts of these antibiotics in manure pose a potential threat to public and ecological health as a result of the potential for them to be released to the environment following land application of manure from animals treated with antibiotics.We investigated the degradation of OTC and TC during anaerobic composting.We tested the effects of temperature and antibiotic concentration on degradation rates in a control and in manure spiked with TCs.We examined changes in p H,biological degradation material(BDM),and moisture corresponding with antibiotic degradation of TCs in the swine manure.Results showed that the OTC and TC concentrations decreased by between 68.54% and 95.50% in all nine treatments following 14 days of anaerobic composting,and the highest removal ef ficiencies were observed at an incubation temperature of 55°C and initial concentrations of 10.36 μg·g^(-1),and 5.96 μg·g^(-1) of OTC and TC,respectively,which were degraded by 95.50%,and 90.06%.During composting at 55 °C and at added concentrations of 5 μg·g^(-1),OTC decreased rapidly,and the time required for50% and 90% degradation was 4.1 and 9.8 days,respectively;for TC,these values were 4.4 and 14.0 days,respectively.Removal ef ficiencies for all TCs correlated well with moisture content of the manure.These results show that composting may be a practical and useful means to reduce concentrations of OTC and TC in swine manure prior to its land application.
基金The authors acknowledge the financial support from the National Natural Science Foundation of China(No.42077335).
文摘Recently increasing concerns from the scientists and public have been paid for seawater pollution due to tetracycline(TC)overuse in maricultural area.However,there are few methods or instruments that can be used for specific and rapid detection of this antibiotic in seawater.In this study,the colloidal gold immunochromatographic assay(CG-ICA)was used to achieve this goal.A commercialized monoclonal antibody against TC(anti-TC mAb)was selected because of its higher sensitivity(half-maximal inhibitory concentration of 2.38μgL^(-1)).The prepared CG particles(average diameter of 20 nm)were used to label anti-TC mAb at pH 8.0.The conjugate pad was formed by spraying the CG-labeled anti-TC mAb on a glass fibre membrane followed by proper dryness.The test pad was made by immobilizing artificial antigen and anti-mouse mAb in the test line and the control line,respectively,in a nitrocellulose membrane.The test strip,assembled with sample pad,conjugate pad,test pad and absorbent pad,could be used to detect TC during seawater sample flowing through these components in turn.The results could be observed by the naked eye in 10min.The visible limit of detection(vLOD)was 20μgL^(-1) for TC in seawater.The CG-ICA test results were in good agreement with those of liquid chromatography-tandem mass spectrometry(LC-MS/MS).The assay also showed that,oxytetracycline(OTC)and chlortetracycline(CTC),as the structural analogues of TC,did not interfere with TC determination.Furthermore,the TC concentration given by test strip could not be affected by the fluctuation of temperature(10℃–30℃),pH(7–9)and salinity(0–40)of seawater.Therefore,CG-ICA is a suitable tool for rapid,on-site,and semi-quantitative detection of TC in seawater.
文摘The presence of tetracyclines in soil and surface water is an emerging concern. The present study was undertaken to investigate remediation of tetracylines (tetracycline (TC), oxytetracycline (OTC) and chlortetracycline (CTC)) from aqueous solution using vetiver grass, water lettuce, and sunflower and root exudates of water lettuce, sunflower and from soil by tomato, Indian mustard and carrot plant. The data of this study denote that vetiver grass, water lettuce, sunflower remedy tetracyclines from water. The remediation % after 63 days of treatment was 87-61 for TC;88-68 for OTC and 87-68 for CTC. The remediation of tetracyclines at lower concentration of antibiotics in presence of root exudates of water lettuce and sunflower was more than 99% and remediation was faster than water lettuce or sunflower. The remediation of tetracyclines from aqueous solution may be due to oxidation of-OH group(s) of tetracycline through a process that is thought to involve reactive oxygen intermediates and/or role of peroxidase enzyme. The plant crops viz., tomato, Indian mustard and carrot can remedy 41%-72% of amended tetracyclines. The maximum bioaccumulation of TC and CTC was in Indian mustard and OTC was maximally bioaccumulated in tomato.
文摘A coaxial dielectric barrier discharge (DBD) reactor was used for plasma-catalytic degradation of tetracycline hydrochloride over a series of Mn/γ-Al2O3 catalysts prepared by the incipient wetness impregnation method.The combination of plasma and the Mn/γ-Al2O3 catalysts significantly enhanced the degradation efficiency of tetracycline hydrochloride compared to the plasma process alone,with the 10% Mn/γ-Al2O3 catalyst exhibiting the best tetracycline hydrochloride degradation efficiency.A maximum degradation efficiency of 99.3% can be achieved after 5 min oxidation and a discharge power of 1.3 W,with only 69.7% by a single plasma process.The highest energy yield of the plasma-catalytic process is 91.7 g kWh-1.Probable reaction mechanisms of the plasma-catalytic removal of tetracycline hydrochloride were also proposed.
文摘Honey is being used as a pure natural and as an ingredient in many foods, pharmaceuticals and cosmetics than ever before, so honey testing has become essential to maintain its healthful characteristics and protect public health. Tetracyclines （TCs） are used for the treatment or prevention of American and European foul broad in bee colonies which are caused especially by two species of bacteria--Paenibacillus larvae and Melissococcus pluton. The present study aimed to determine the tetracycline residues in different types of local and imported honey. The reversed-phase high performance liquid chromatography （HPLC） with ultraviolet detection （UV） was used. Tetracycline and oxytetracycline were extracted from honey with 0.01 M sodium succinate buffer. Of the examined samples, 31.25% and 12.50% had these oxytetracycline and tetracycline residues, respectively. It was observed that all the samples studied have been reported residues of tetracycline and oxytetracycline more than international standards of maximum residue limit （MRLs）.
文摘The fluorescence of tetracycline was greatly enhanced by chelation with Eu 3+ in Tris HCl buffer (pH8.5). The addition of cetyltrimethylammonium bromide, a cationic surfactant, further enhanced the fluorescence of tetracycline Eu 3+ chelates.This spectrofluorimetric method for the determination of tetracycline yielded a linear responeses in the range of 10 nmol/L 10 μmol/L. The detection limit was 5 nmol/L (or 2.22 ng/mL).The recovery of tetracycline spiked in serum at levels of l and 0.09 μg/mL was 97.9% and 97.8%, respectively. This method is fast, sensitive and suitable for the determination of tetracycline in biological specimens.
基金Project Supported by the National Natural Science Fundation of China.
文摘The Eu-Tetracycline(TC)-TOPO-sodium dodecyl sulfonate(SDS)system was studied,Experiments showed that the maximum fluorescence intensity was obtained in the pH range of 7.5-8.7 and the concentrations of Eu^(a+),TOPO and SDS are 1.0×10^(-6)mol/L,1.0×10^(-8)mol/L and 1.0×10^(-8)mol/L,respectively,This fluorescence system can be used for the determination of TC in serum and urine,Beer's Law is obeyed in the range of 2.0×10^(-8)mol/L=1.0×10^(-6)mol/L for the concentration of TC. The determination limit is 1.2×10^(-8)mol/L,The composition and the luminescence mechanism were discussed.
文摘[Objective]The aim is to provide data support for the prevention and control of pollution of exogenous chemical in pig breeding industry.[Methods]Solid-phase extraction（SPE）and tandem mass（LC-MS/MS）were used for determining four classes（tetracyclines,quinolones,macrolides and sulfonamides）of 10 common veterinary antibiotics in groundwater of an important pig breeding base in the Yangtze River Delta region of China.[Results]Results revealed the total concentration of the ten antibiotics in urban rivers ranged from 20.1 ng /L to 61.2 ng /L.The highest proportion was taken by tetracyclines,account for 95%,the concentration was up to 44.0 ng /L.Quinolones shared the second largest proportion of total concentration,about 29%,the concentration was up to 21.6 ng /L,while concentration of sulfonamides and macrolides were respectively below 2.7 ng /L and 6.3 ng /L.The highest total concentration of the ten antibiotics in rural rivers was up to 467 ng /L,60% of which was shared by tetracyclines,the highest concentration of tetracyclines was 253 ng /L.Sulfonamides share 20% of the total concentration,the highest concentration of it was 165 ng /L.The highest concentration of macrolides and quinolones was 14.6 ng /L and 14.5 ng /L,respectively.[Conclusion] Antibiotics＇ pollution in town rivers is more serious than urban rivers＇ pollution caused by antibiotics.
