OBJECTIVE To observe effects of tetrahydroxystilbene glucoside(TSG)on behavior,content of dopamine and its metabolites in striatum of Parkinson′s disease(PD)model mice induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydrop...OBJECTIVE To observe effects of tetrahydroxystilbene glucoside(TSG)on behavior,content of dopamine and its metabolites in striatum of Parkinson′s disease(PD)model mice induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP)injection.METHODS Mice were randomly divided into control group,model group,TSG low dose(60mg·kg-1)and high dose(120mg·kg-1)groups.The behavior changes of mice were observed by pole test,rotarod test and spontaneous movement test.The tyrosine hydroxylase(TH)positive cells were detected by immunohistochemical method.The content of dopamine(DA)and its metabolites in striatum were determined by HPLC-ECD.RESULTS MPTP model mice showed behavior deficit.The number of TH positive neurons in substantia nigra,the content of dopamine and its metabolites in striatum in model mice decreased significantly compared with control group.TSG ameliorated mice behavior,increased the number of TH positive neurons in the substantia nigra about 18.8%,and elevated the content of dopamine in striatum about 34.5% compared with model mice.CONCLUSION TSG protected dopaminergic neurons against MPTP-induced damage,and may become a candidate drug for prevention and treatment of Parkinson′s disease.展开更多
Objective:Mitochondrial dysfunction is evident in the early stage of Alzheimer's disease(AD).Therefore development of drugs that protect mitochondrial function is a promising strategy for AD.The present work was t...Objective:Mitochondrial dysfunction is evident in the early stage of Alzheimer's disease(AD).Therefore development of drugs that protect mitochondrial function is a promising strategy for AD.The present work was to investigate the effects of 2,3,5,4'-Tetrahydroxystilbene-2-O-β-d-glucosides(TSG) on a mitochondrial dysfunction cell model induced by sodium azide and elucidate the underlying mechanisms.Methods:Mitochondrial membrane potential(MMP) was detected by a fluorescence method.Cellular adenosine triphosphate(ATP) level was measured using a firefly luciferase-based kit.Reactive oxygen species(ROS) was detected using dichlorofluorescin diacetate(DCFH-DA).The expression levels of Bcl-2 and Bax were measured by Western blotting assay.Flow cytometry was utilized to measure apoptosis.Results:Pretreatment of TSG(25-200 μmol/L) for 24 h significantly elevated MMP and ATP content,reduced ROS level and Bax/Bcl-2 ratio,and inhibited apoptosis in SH-SY5 Y cells exposed to sodium azide.Conclusion:These results suggest that TSG protects SH-SY5 Y cells against sodium azide-induced mitochondrial dysfunction and apoptosis.These findings are helpful to understand the protective effect of TSG on mitochondria,which are involved in the early stage of AD.展开更多
Objective：To investigate the effect of 2,3,4’,5-tetrahydroxystilbene-2-0- p-D-glucoside（TSG）,an active component extracted from the root of Polygonum multiflorum,on angiotensin Ⅱ（Ang Ⅱ）-induced proliferation o...Objective：To investigate the effect of 2,3,4’,5-tetrahydroxystilbene-2-0- p-D-glucoside（TSG）,an active component extracted from the root of Polygonum multiflorum,on angiotensin Ⅱ（Ang Ⅱ）-induced proliferation of cultured rat vascular smooth muscle cells（VSMCs） and to identify the potential mechanism.Methods：Cell proliferation and cell cycle were determined by cell counting,5-bromo-2’-deoxyuridine incorporation assay,proliferating cell nuclear antigen protein expression and flow cytometry.Levels of phosphorylated extracellular signal-regulated kinase 1/2（ERK1/2）,mitogenic extracellular kinase 1/2（MEK1/2） and Src in VSMCs were measured by Western blot.The expression of c-fos,c-jun and c-myc mRNA were measured by reverse transcription polymerase chain reaction（RT-PCR）.Intracellular reactive oxygen species（ROS） was measured by fluorescence assay.Results：TSG significantly inhibited Ang Ⅱ-induced VSMCs proliferation and arrested cells in the G1/S checkpoint（P〈0.05 or P〈0.01）.TSG decreased the levels of phosphorylated ERK1/2,MEK1/2 and Src in VSMCs（P〈0.05 or P〈0.01）.TSG also suppressed c-fos,c-jun and c-myc mRNA expression（P〈0.05 or P〈0.01）.In addition,the intracellular ROS was reduced by TSG（P〈0.01）.Conclusions：TSG inhibited Ang Ⅱ-induced VSMCs proliferation.Its antiproliferative effect might be associated with down-regulation of intracellular ROS,followed by the suppression of the Src-MEK1/2-ERK1/2 signal pathway,and hence,blocking cell cycle progression.展开更多
目的建立能同时测定冠心安口服液中蒙花苷、延胡索乙素和2,3,5,4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷含量的HPLC分析方法。方法Capcell Pak UG C_(18)色谱柱分离;蒙花苷、延胡索乙素和2,3,5,4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷检测...目的建立能同时测定冠心安口服液中蒙花苷、延胡索乙素和2,3,5,4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷含量的HPLC分析方法。方法Capcell Pak UG C_(18)色谱柱分离;蒙花苷、延胡索乙素和2,3,5,4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷检测波长分别设定为334、281、320 nm;柱温为室温;进样体积为10μL;以乙腈为流动相A,1 mL·L^(−1)磷酸溶液(三乙胺调节pH至6.0)为流动相B,梯度洗脱。结果蒙花苷、延胡索乙素和2,3,5,4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷的质量浓度分别在0.0245~2.4500、0.0457~4.5700、0.0474~4.7400μg·mL^(−1)范围内线性良好;平均回收率分别为100.8%、99.9%、101.4%;精密度和重复性RSD值(n=6)均在5.0%以内;供试品溶液在24 h内稳定。结论此方法具有前处理简单、分析时间短和检测结果准确等优点,适用于冠心安口服液制剂的质量控制。展开更多
目的对比研究顺式和反式二苯乙烯苷的降血脂作用。方法分别考察顺式、反式二苯乙烯苷对正常小鼠、慢性高血脂小鼠和急性高血脂小鼠血清中总胆固醇(total cholesterol,TC)、甘油三酯(triglyceride,TG)、高密度脂蛋白胆固醇(high density ...目的对比研究顺式和反式二苯乙烯苷的降血脂作用。方法分别考察顺式、反式二苯乙烯苷对正常小鼠、慢性高血脂小鼠和急性高血脂小鼠血清中总胆固醇(total cholesterol,TC)、甘油三酯(triglyceride,TG)、高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)和低密度脂蛋白胆固醇(low density lipoprotein cholesterol,LDL-C)含量的影响。结果顺式二苯乙烯苷能显著性降低高血脂小鼠血清中TC、TG及LDL-C的水平,也能显著性降低正常血脂小鼠血清中LDL-C的含量,高剂量顺式二苯乙烯苷还能显著性升高正常血脂小鼠和高血脂小鼠血清中HDL-C的水平。顺式二苯乙烯苷对正常小鼠和急性高血脂小鼠的降血脂作用与反式基本相同,但对慢性高血脂小鼠血浆中TC和TG的降低作用明显强于反式二苯乙烯苷。结论顺式二苯乙烯苷具有比反式二苯乙烯苷更好的降血脂作用。展开更多
基金The project supported by National Natural Science Foundation of China(81273498)the Capital Health Research and Development Foundation(2011-1001-04)
文摘OBJECTIVE To observe effects of tetrahydroxystilbene glucoside(TSG)on behavior,content of dopamine and its metabolites in striatum of Parkinson′s disease(PD)model mice induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP)injection.METHODS Mice were randomly divided into control group,model group,TSG low dose(60mg·kg-1)and high dose(120mg·kg-1)groups.The behavior changes of mice were observed by pole test,rotarod test and spontaneous movement test.The tyrosine hydroxylase(TH)positive cells were detected by immunohistochemical method.The content of dopamine(DA)and its metabolites in striatum were determined by HPLC-ECD.RESULTS MPTP model mice showed behavior deficit.The number of TH positive neurons in substantia nigra,the content of dopamine and its metabolites in striatum in model mice decreased significantly compared with control group.TSG ameliorated mice behavior,increased the number of TH positive neurons in the substantia nigra about 18.8%,and elevated the content of dopamine in striatum about 34.5% compared with model mice.CONCLUSION TSG protected dopaminergic neurons against MPTP-induced damage,and may become a candidate drug for prevention and treatment of Parkinson′s disease.
基金supported by the National Natural Science Foundation of China (Nos.81273498, 81341087)National Science and Technology Major Project of China (No.2015ZX09101-016)。
文摘Objective:Mitochondrial dysfunction is evident in the early stage of Alzheimer's disease(AD).Therefore development of drugs that protect mitochondrial function is a promising strategy for AD.The present work was to investigate the effects of 2,3,5,4'-Tetrahydroxystilbene-2-O-β-d-glucosides(TSG) on a mitochondrial dysfunction cell model induced by sodium azide and elucidate the underlying mechanisms.Methods:Mitochondrial membrane potential(MMP) was detected by a fluorescence method.Cellular adenosine triphosphate(ATP) level was measured using a firefly luciferase-based kit.Reactive oxygen species(ROS) was detected using dichlorofluorescin diacetate(DCFH-DA).The expression levels of Bcl-2 and Bax were measured by Western blotting assay.Flow cytometry was utilized to measure apoptosis.Results:Pretreatment of TSG(25-200 μmol/L) for 24 h significantly elevated MMP and ATP content,reduced ROS level and Bax/Bcl-2 ratio,and inhibited apoptosis in SH-SY5 Y cells exposed to sodium azide.Conclusion:These results suggest that TSG protects SH-SY5 Y cells against sodium azide-induced mitochondrial dysfunction and apoptosis.These findings are helpful to understand the protective effect of TSG on mitochondria,which are involved in the early stage of AD.
基金Supported by the Natural Science Foundation of Jiangsu Province(No.BK2009162)the Natural Science Foundation of the Jiangsu Higher Education Institutions of China(No.09KJB310010)the Priority Academic Program Development of Jiangsu Higher Education Institutions
文摘Objective：To investigate the effect of 2,3,4’,5-tetrahydroxystilbene-2-0- p-D-glucoside（TSG）,an active component extracted from the root of Polygonum multiflorum,on angiotensin Ⅱ（Ang Ⅱ）-induced proliferation of cultured rat vascular smooth muscle cells（VSMCs） and to identify the potential mechanism.Methods：Cell proliferation and cell cycle were determined by cell counting,5-bromo-2’-deoxyuridine incorporation assay,proliferating cell nuclear antigen protein expression and flow cytometry.Levels of phosphorylated extracellular signal-regulated kinase 1/2（ERK1/2）,mitogenic extracellular kinase 1/2（MEK1/2） and Src in VSMCs were measured by Western blot.The expression of c-fos,c-jun and c-myc mRNA were measured by reverse transcription polymerase chain reaction（RT-PCR）.Intracellular reactive oxygen species（ROS） was measured by fluorescence assay.Results：TSG significantly inhibited Ang Ⅱ-induced VSMCs proliferation and arrested cells in the G1/S checkpoint（P〈0.05 or P〈0.01）.TSG decreased the levels of phosphorylated ERK1/2,MEK1/2 and Src in VSMCs（P〈0.05 or P〈0.01）.TSG also suppressed c-fos,c-jun and c-myc mRNA expression（P〈0.05 or P〈0.01）.In addition,the intracellular ROS was reduced by TSG（P〈0.01）.Conclusions：TSG inhibited Ang Ⅱ-induced VSMCs proliferation.Its antiproliferative effect might be associated with down-regulation of intracellular ROS,followed by the suppression of the Src-MEK1/2-ERK1/2 signal pathway,and hence,blocking cell cycle progression.
文摘目的建立能同时测定冠心安口服液中蒙花苷、延胡索乙素和2,3,5,4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷含量的HPLC分析方法。方法Capcell Pak UG C_(18)色谱柱分离;蒙花苷、延胡索乙素和2,3,5,4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷检测波长分别设定为334、281、320 nm;柱温为室温;进样体积为10μL;以乙腈为流动相A,1 mL·L^(−1)磷酸溶液(三乙胺调节pH至6.0)为流动相B,梯度洗脱。结果蒙花苷、延胡索乙素和2,3,5,4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷的质量浓度分别在0.0245~2.4500、0.0457~4.5700、0.0474~4.7400μg·mL^(−1)范围内线性良好;平均回收率分别为100.8%、99.9%、101.4%;精密度和重复性RSD值(n=6)均在5.0%以内;供试品溶液在24 h内稳定。结论此方法具有前处理简单、分析时间短和检测结果准确等优点,适用于冠心安口服液制剂的质量控制。
文摘目的对比研究顺式和反式二苯乙烯苷的降血脂作用。方法分别考察顺式、反式二苯乙烯苷对正常小鼠、慢性高血脂小鼠和急性高血脂小鼠血清中总胆固醇(total cholesterol,TC)、甘油三酯(triglyceride,TG)、高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)和低密度脂蛋白胆固醇(low density lipoprotein cholesterol,LDL-C)含量的影响。结果顺式二苯乙烯苷能显著性降低高血脂小鼠血清中TC、TG及LDL-C的水平,也能显著性降低正常血脂小鼠血清中LDL-C的含量,高剂量顺式二苯乙烯苷还能显著性升高正常血脂小鼠和高血脂小鼠血清中HDL-C的水平。顺式二苯乙烯苷对正常小鼠和急性高血脂小鼠的降血脂作用与反式基本相同,但对慢性高血脂小鼠血浆中TC和TG的降低作用明显强于反式二苯乙烯苷。结论顺式二苯乙烯苷具有比反式二苯乙烯苷更好的降血脂作用。
