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Tetramycin抗性基因克隆及生物活性的测定 被引量:2
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作者 陈飞 吴红艳 +3 位作者 桓明辉 关艳丽 郭玲玲 于广峰 《微生物学杂志》 CAS CSCD 2012年第1期17-22,共6页
为研究Tetramycin生物合成基因簇,提高产量,根据已知链霉菌抗性基因的保守核苷酸序列设计简并引物,以链霉菌11371基因组DNA为模板进行克隆Tetramycin抗性基因,并将其转化GS115中表达,以阳性克隆为指示菌,测定Tetramycin抗性基因生物活... 为研究Tetramycin生物合成基因簇,提高产量,根据已知链霉菌抗性基因的保守核苷酸序列设计简并引物,以链霉菌11371基因组DNA为模板进行克隆Tetramycin抗性基因,并将其转化GS115中表达,以阳性克隆为指示菌,测定Tetramycin抗性基因生物活性。结果显示克隆的Tetramycin抗性基因1-2-1和2-1-1测定生物活性时并没有表现出对Tetramycin拮抗作用的提高,但为进一步研究Tetramycin生物合成基因簇、提高Tetramycin产量提供理论数据。 展开更多
关键词 链霉菌 tetramycin 抗性基因
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Rice Resistance against Blast Induced by Tetramycin 被引量:1
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作者 钟丽娟 赵新海 +2 位作者 张庆华 徐冲 朱会林 《Plant Diseases and Pests》 CAS 2010年第3期6-8,27,共4页
[ Objective] The research aimed to discuss the tetramycin-inducad resistance and its mechanism in order to provide basis for better application in the production. [ Methedl Taking rice blast as the control object, the... [ Objective] The research aimed to discuss the tetramycin-inducad resistance and its mechanism in order to provide basis for better application in the production. [ Methedl Taking rice blast as the control object, the impacts of tetramycin on the activity of PAL, POD, PPO and SOD in rice leaves were studied. [Result] The activity of PAL, POD and PPO in rice leaves were significantly increased after spraying tetramycin. The activity of enzyme in the treatment of inoculation after spraying terramycin for 24 h increased most obviously. The activity of SOD had no significant difference among different treatments. [ Condesion] Terramycin not only has strong antimicrobial effect, but also might enhance disease resistance in order to prevent pathogen infection through inducing the activity of rice defense enzyme. 展开更多
关键词 tetramycin Rice blast PaL POD PPO SOD
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Effects of Integrated Technology of Medicine and Fertilizer on Bacterial Wilt of Tobacco
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作者 ZHOU Lian-chuan HE Wen-wei +5 位作者 QIN Xi-yun XU Tian-yang ZHANG Chuan-shu WANG Jing LU Fu-zhi ZHANG Li-xia 《Agricultural Science & Technology》 CAS 2019年第3期47-50,共4页
In order to validate the control effects of integrated technology of medicine and fertilizer on tobacco bacterial wilt,field experiments were conducted by using youmeili and tetracycline as the test agents.The results... In order to validate the control effects of integrated technology of medicine and fertilizer on tobacco bacterial wilt,field experiments were conducted by using youmeili and tetracycline as the test agents.The results obtained were listed as follows:(1)The incidence rate of bacterial wilt ascended more quickly after July 10 th,the incidence of CK reached 78.30%on July 25 th.(2)The control effect of“youmeili 3 g+tetramycin 200 times liquid”reached 69.47%,significantly higher than other treatments.(3)The output value of“Youmeil 3 g+tetramycin 200 times liquid”served as 1635.66 yuan/667m^2,with the highest economic character in all treatments.In a word,integrated technology of medicine and fertilizer was a feasible approach to control tobacco bacterial wilt. 展开更多
关键词 Bacterial wilt Control effect Youmeil+tetramycin Integrated technology of medicine and fertilizer
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