This study was conducted to investigate ovule abortion of tetraploid Robinia pseudoacacia and its reasons. It was found that in the ovule development period of tetraploid R. pseudoacacia from 15 d after coronal openin...This study was conducted to investigate ovule abortion of tetraploid Robinia pseudoacacia and its reasons. It was found that in the ovule development period of tetraploid R. pseudoacacia from 15 d after coronal opening, the levels of growth-promoting endogenous hormones in abortive ovules were lower than those in normal ovules, and the level of a growth-inhibiting hormone was always higher than those in normal ovules, indicating that ovule abortion of tetraploid R. pseudoacacia might be related to abnormal endogenous phytohormones in ovules. This study laid a foundation for further study on highly sterile phenomenon of tetraploid R. pseudoacacia seeds.展开更多
Preparation of silage is a common method to preserve green forage. It plays an important role in improving forage utilization, solving the problem of forage provision and meeting the nutritional needs of livestock in ...Preparation of silage is a common method to preserve green forage. It plays an important role in improving forage utilization, solving the problem of forage provision and meeting the nutritional needs of livestock in winter and spring. The effects of various supplements on tetraploid black locust （Robinia pseudoacacia L.） silage were studied by analyzing its color, odor, texture, pH value, the composition and amount of organic acids, the ammonia-N/total-N ratio, crude protein, neutral detergent fibers and acid detergent fibers. Our results show that the silage quality of wilted tetraploid R. pseudoacacia preserved alone is acceptable, while adding brown sugar and Yishengkang to tetraploid R. pseudoacacia foliage improved its quality. The treatments consisting of the tet- raploid R. pseudoacacia mixed with 33% and 50% corn stalk achieved the best silage quality in terms ofpH value （p 〈 0.01）, amount of lactic acid （p 〈 0.01）, the ammonia-N/total-N ratio （p 〈 0.01） and the fermentation quality. These treatments also resulted in a high content of crude protein and lower amounts of acid detergent fibers. The treatment consisting of the tetraploid R. pseudoacacia mixed with 20% corn stalk was also good in the quality of its fermentation.展开更多
Lectins are natural proteins in animals,plants,and microorganisms and can be divided into 12 families.These lectins play important roles in various environmental stresses.Some polyploid plants show tolerance to enviro...Lectins are natural proteins in animals,plants,and microorganisms and can be divided into 12 families.These lectins play important roles in various environmental stresses.Some polyploid plants show tolerance to environmental stresses and to insect pests.However,the mechanism of stress tolerance is unclear.Tetraploid Robinia pseudoacacia(4×)under salt stress showed higher tolerance than diploid R.pseudoacacia(2×).As lectin can improve stress tolerance,it was questioned whether the stress resistance of polyploid plants was related to the lectin protein.In this study,salt resistance of lectin gene TRpL1 was verified by its over-expression in plants.In addition,salt resistance of lectin protein by E.coli strains was detected.The data revealed that the over-expression transgenic plants of TRpL1 showed better salt tolerance than control plants under salt stress,and the TRpL1-expressing strain also grew better in the medium with added NaCl.Therefore,tetraploid plants can resist salt stress through TRpL1 protein regulation.展开更多
文摘This study was conducted to investigate ovule abortion of tetraploid Robinia pseudoacacia and its reasons. It was found that in the ovule development period of tetraploid R. pseudoacacia from 15 d after coronal opening, the levels of growth-promoting endogenous hormones in abortive ovules were lower than those in normal ovules, and the level of a growth-inhibiting hormone was always higher than those in normal ovules, indicating that ovule abortion of tetraploid R. pseudoacacia might be related to abnormal endogenous phytohormones in ovules. This study laid a foundation for further study on highly sterile phenomenon of tetraploid R. pseudoacacia seeds.
基金supported by the State Forestry Administration of China (Nos.2003-5-2 and 2004-04)
文摘Preparation of silage is a common method to preserve green forage. It plays an important role in improving forage utilization, solving the problem of forage provision and meeting the nutritional needs of livestock in winter and spring. The effects of various supplements on tetraploid black locust （Robinia pseudoacacia L.） silage were studied by analyzing its color, odor, texture, pH value, the composition and amount of organic acids, the ammonia-N/total-N ratio, crude protein, neutral detergent fibers and acid detergent fibers. Our results show that the silage quality of wilted tetraploid R. pseudoacacia preserved alone is acceptable, while adding brown sugar and Yishengkang to tetraploid R. pseudoacacia foliage improved its quality. The treatments consisting of the tet- raploid R. pseudoacacia mixed with 33% and 50% corn stalk achieved the best silage quality in terms ofpH value （p 〈 0.01）, amount of lactic acid （p 〈 0.01）, the ammonia-N/total-N ratio （p 〈 0.01） and the fermentation quality. These treatments also resulted in a high content of crude protein and lower amounts of acid detergent fibers. The treatment consisting of the tetraploid R. pseudoacacia mixed with 20% corn stalk was also good in the quality of its fermentation.
基金supported by the National Natural Science Foundation of China(32071728)。
文摘Lectins are natural proteins in animals,plants,and microorganisms and can be divided into 12 families.These lectins play important roles in various environmental stresses.Some polyploid plants show tolerance to environmental stresses and to insect pests.However,the mechanism of stress tolerance is unclear.Tetraploid Robinia pseudoacacia(4×)under salt stress showed higher tolerance than diploid R.pseudoacacia(2×).As lectin can improve stress tolerance,it was questioned whether the stress resistance of polyploid plants was related to the lectin protein.In this study,salt resistance of lectin gene TRpL1 was verified by its over-expression in plants.In addition,salt resistance of lectin protein by E.coli strains was detected.The data revealed that the over-expression transgenic plants of TRpL1 showed better salt tolerance than control plants under salt stress,and the TRpL1-expressing strain also grew better in the medium with added NaCl.Therefore,tetraploid plants can resist salt stress through TRpL1 protein regulation.
